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Ramesh, Kirti; Hu, Marian Y; Thomsen, Jörn; Bleich, Markus; Melzner, Frank (2017): Seawater carbonate chemistry and calcifying fluid carbonate chemistry, shell length of Mussel larvae [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.891437

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Abstract:
Understanding mollusk calcification sensitivity to ocean acidification (OA) requires a better knowledge of calcification mechanisms. Especially in rapidly calcifying larval stages, mechanisms of shell formation are largely unexplored—yet these are the most vulnerable life stages. Here we find rapid generation of crystalline shell material in mussel larvae. We find no evidence for intracellular CaCO3 formation, indicating that mineral formation could be constrained to the calcifying space beneath the shell. Using microelectrodes we show that larvae can increase pH and [CO3]2−beneath the growing shell, leading to a ~1.5-fold elevation in calcium carbonate saturation state (Omega arag). Larvae exposed to OA exhibit a drop in pH, [CO3]2− and Omega arag at the site of calcification, which correlates with decreased shell growth, and, eventually, shell dissolution. Our findings help explain why bivalve larvae can form shells under moderate acidification scenarios and provide a direct link between ocean carbonate chemistry and larval calcification rate.
Keyword(s):
Acid-base regulation; Animalia; Baltic Sea; Baltic Sea; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Mollusca; Mytilus edulis; Single species; Temperate
Related to:
Ramesh, Kirti; Hu, Marian Y; Thomsen, Jörn; Bleich, Markus; Melzner, Frank (2017): Mussel larvae modify calcifying fluid carbonate chemistry to promote calcification. Nature Communications, 8(1), https://doi.org/10.1038/s41467-017-01806-8
Original version:
Ramesh, Kirti; Hu, Marian Y; Thomsen, Jörn; Bleich, Markus; Melzner, Frank (2017): Carbonate chemistry at the site of mineralization in mussel larvae. PANGAEA, https://doi.org/10.1594/PANGAEA.881869
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb
Project(s):
Ocean Acidification International Coordination Centre (OA-ICC)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2018-05-23.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1TypeTypeRamesh, Kirtistudy
2SpeciesSpeciesRamesh, Kirti
3Registration number of speciesReg spec noRamesh, Kirti
4Uniform resource locator/link to referenceURL refRamesh, KirtiWoRMS Aphia ID
5ExperimentExpRamesh, Kirti
6Time in hoursTimehRamesh, Kirtipost fertilization
7ReplicateReplRamesh, Kirti
8Calcium per individualCa2+µmol/#Ramesh, Kirti
9Carbonate ion[CO3]2-µmol/kgRamesh, Kirtiseawater
10Carbonate ion[CO3]2-µmol/kgRamesh, Kirticalcification space
11deltadeltaRamesh, KirtiDelta [CO32-]
12pHpHRamesh, Kirtiseawater
13pHpHRamesh, Kirticalcification space
14deltadeltaRamesh, KirtiDelta pH (CS-SW)
15CalciumCa2+mmol/kgRamesh, Kirtiseawater
16CalciumCa2+mmol/kgRamesh, Kirticalcification space
17deltadeltaRamesh, KirtiDelta Calcium [Ca2+]
18FluorescenceFluorescencearbitrary unitsRamesh, Kirtishell
19Shell lengthShell lmmRamesh, Kirti
20Shell length, standard deviationShell l std dev±Ramesh, Kirti
21SalinitySalRamesh, Kirti
22Temperature, waterTemp°CRamesh, Kirti
23Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmRamesh, Kirti
24Carbon, inorganic, dissolvedDICµmol/kgRamesh, Kirti
25pHpHRamesh, KirtiNBS scale
26Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmRamesh, Kirti
27Alkalinity, totalATµmol/kgRamesh, Kirti
28Carbonate ion[CO3]2-µmol/kgRamesh, Kirti
29Aragonite saturation stateOmega ArgRamesh, Kirti
30Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
31pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
32Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
33Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
34Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
35Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
36Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
37Alkalinity, totalATµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
38Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
39Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
13036 data points

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