Citation:

jeb.172643

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Abstract:

Salinity change in coastal zone is a common phenomena which can put several coastal soft bottom organisms under stressed condition. These stress could be mediated through their mitochondrial functions. In this data set, we present the effect of acclimation to three different salinity regime (normal: 15 psu, low: 5 psu and fluctuating: daily salinity cycle between 5 and 15 psu) on the mitochondrial performance (MO2) of soft shell clam, Mya arenaria. Clams were acclimated to normal, low and fluctuating salinity; the mitochondrial respiration of these clams was then measured under two different osmolarity (450 and 150 mOsm, representing 15 and 5 psu, respectively). MO2 was measured sequentially, starting with the addition of pyruvate and malate, succinate, ADP, oligomycin, CCCP, rotenon, antimycin A, TMPD and ascorbate, and finally KCN.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Comment
1	Regime	Regime		Haider, Fouzia	acclimated salinity regime
2	Salinity, minimum	Sal min		Haider, Fouzia	acclimated, in PSU
3	Salinity, maximum	Sal max		Haider, Fouzia	acclimated, in PSU
4	Temperature, water	Temp	°C	Haider, Fouzia	acclimated
5	Temperature, water	Temp	°C	Haider, Fouzia	assay
6	Osmotic concentration	Osmolarity	mOsmol/l	Haider, Fouzia	assay
7	Sample ID	Sample ID		Haider, Fouzia
8	Ratio	Ratio		Haider, Fouzia	respiratory control ratio
9	Proteins	Protein	mg/ml	Haider, Fouzia	mito suspension
10	Proteins	Protein	mg/ml	Haider, Fouzia	assay
11	Mitochondria, oxygen consumption	Mitochondria O2 con	µmol/g/min	Haider, Fouzia	adding Pyruvate+Malate
12	Mitochondria, oxygen consumption	Mitochondria O2 con	µmol/g/min	Haider, Fouzia	adding Succinate
13	Mitochondria, oxygen consumption	Mitochondria O2 con	µmol/g/min	Haider, Fouzia	adding ADP
14	Mitochondria, oxygen consumption	Mitochondria O2 con	µmol/g/min	Haider, Fouzia	adding Oligomycin
15	Mitochondria, oxygen consumption	Mitochondria O2 con	µmol/g/min	Haider, Fouzia	adding CCCP
16	Mitochondria, oxygen consumption	Mitochondria O2 con	µmol/g/min	Haider, Fouzia	adding Rotenon
17	Mitochondria, oxygen consumption	Mitochondria O2 con	µmol/g/min	Haider, Fouzia	adding Antimycin A
18	Mitochondria, oxygen consumption	Mitochondria O2 con	µmol/g/min	Haider, Fouzia	adding TMPD+Ascorbate
19	Mitochondria, oxygen consumption	Mitochondria O2 con	µmol/g/min	Haider, Fouzia	adding KCN

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Size:

798 data points

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