Citation:

jeb.172643

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Abstract:

Salinity change in coastal zone is a common phenomena which can put several coastal soft bottom organisms under stressed condition. These stress could be mediated through their mitochondrial functions. In this data set, we present the effect of acclimation to three different salinity regime (normal: 15 psu, low: 5 psu and fluctuating: daily salinity cycle between 5 and 15 psu) on the mitochondrial performance (MO2) of soft shell clam, Mya arenaria. Clams were acclimated to normal, low and fluctuating salinity; the mitochondrial respiration of these clams was then measured under two different osmolarity (450 and 150 mOsm, representing 15 and 5 psu, respectively). MO2 was measured sequentially, starting with the addition of pyruvate and malate, succinate, ADP, oligomycin, CCCP, rotenon, antimycin A, TMPD and ascorbate, and finally KCN.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Comment
1	Regime	Regime		Haider, Fouzia	acclimated salinity regime
2	Salinity, minimum	Sal min		Haider, Fouzia	acclimated, in PSU
3	Salinity, maximum	Sal max		Haider, Fouzia	acclimated, in PSU
4	Temperature, water	Temp	°C	Haider, Fouzia	acclimated
5	Temperature, water	Temp	°C	Haider, Fouzia	assay
6	Osmotic concentration	Osmolarity	mOsmol/l	Haider, Fouzia	assay
7	Sample ID	Sample ID		Haider, Fouzia
8	Ratio	Ratio		Haider, Fouzia	respiratory control ratio
9	Proteins	Protein	mg/ml	Haider, Fouzia	mito suspension
10	Proteins	Protein	mg/ml	Haider, Fouzia	assay
11	Mitochondria, oxygen consumption	Mitochondria O2 con	µmol/g/min	Haider, Fouzia	adding Pyruvate+Malate
12	Mitochondria, oxygen consumption	Mitochondria O2 con	µmol/g/min	Haider, Fouzia	adding Succinate
13	Mitochondria, oxygen consumption	Mitochondria O2 con	µmol/g/min	Haider, Fouzia	adding ADP
14	Mitochondria, oxygen consumption	Mitochondria O2 con	µmol/g/min	Haider, Fouzia	adding Oligomycin
15	Mitochondria, oxygen consumption	Mitochondria O2 con	µmol/g/min	Haider, Fouzia	adding CCCP
16	Mitochondria, oxygen consumption	Mitochondria O2 con	µmol/g/min	Haider, Fouzia	adding Rotenon
17	Mitochondria, oxygen consumption	Mitochondria O2 con	µmol/g/min	Haider, Fouzia	adding Antimycin A
18	Mitochondria, oxygen consumption	Mitochondria O2 con	µmol/g/min	Haider, Fouzia	adding TMPD+Ascorbate
19	Mitochondria, oxygen consumption	Mitochondria O2 con	µmol/g/min	Haider, Fouzia	adding KCN

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Size:

798 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Regime (acclimated salinity regime)	2 Sal min (acclimated, in PSU)	3 Sal max (acclimated, in PSU)	4 Temp [°C] (acclimated)	5 Temp [°C] (assay)	6 Osmolarity [mOsmol/l] (assay)	7 Sample ID	8 Ratio (respiratory control ratio)	9 Protein [mg/ml] (mito suspension)	10 Protein [mg/ml] (assay)	11 Mitochondria O2 con [µmol/g/min] (adding Pyruvate+Malate)	12 Mitochondria O2 con [µmol/g/min] (adding Succinate)	13 Mitochondria O2 con [µmol/g/min] (adding ADP)	14 Mitochondria O2 con [µmol/g/min] (adding Oligomycin)	15 Mitochondria O2 con [µmol/g/min] (adding CCCP)	16 Mitochondria O2 con [µmol/g/min] (adding Rotenon)	17 Mitochondria O2 con [µmol/g/min] (adding Antimycin A)	18 Mitochondria O2 con [µmol/g/min] (adding TMPD+Ascorbate)	19 Mitochondria O2 con [µmol/g/min] (adding KCN)
Fluctuating	5	15	15	15	150	FS-3	3.52	1.830	0.166	5.557	10.584	37.251	18.410	48.190	38.363	5.708	69.499	11.432
Fluctuating	5	15	15	15	450	FS-3	4.50	1.830	0.166	4.244	8.620	38.787	18.559	53.754	45.873	4.731	69.215	16.404
Fluctuating	5	15	15	15	150	FS-4	2.73	6.500	0.591	1.793	2.576	7.036	3.779	8.756	6.697	1.389	15.289	3.164
Fluctuating	5	15	15	15	450	FS-4	4.75	6.500	0.591	1.308	2.466	11.724	5.229	13.539	11.552	1.475	19.413	3.336
Fluctuating	5	15	15	15	150	FS-5	2.60	4.650	0.423	2.482	4.550	11.848	6.274	14.079	10.472	2.358	19.322	4.101
Fluctuating	5	15	15	15	450	FS-5	3.71	4.650	0.423	1.586	3.755	13.918	6.901	17.549	15.101	2.153	24.121	5.608
Fluctuating	5	15	15	15	150	FS-6	2.89	2.460	0.224	3.197	6.352	18.365	10.618	22.634	19.160	4.041	30.007	5.423
Fluctuating	5	15	15	15	450	FS-6	4.07	2.460	0.224	2.651	5.768	23.468	11.161	30.891	25.985	3.890	36.922	7.039
Fluctuating	5	15	15	15	150	FS-7	2.93	3.540	0.322	2.096	3.715	10.879	5.510	11.381	9.474	2.078	18.014	4.900
Fluctuating	5	15	15	15	450	FS-7	5.01	3.540	0.322	1.357	2.448	12.270	6.279	14.147	12.682	2.700	19.071	5.697
Fluctuating	5	15	15	15	150	FS-8	0.91	4.620	0.420	2.604	1.973	1.789	1.764	2.206	1.985	1.227	7.760	3.834
Fluctuating	5	15	15	15	450	FS-8	1.33	4.620	0.420	1.312	2.270	3.009	2.539	5.128	5.293	2.001	17.374	4.317
Normal	15	15	15	15	150	NS-2	2.07	1.124	0.102	12.633	22.239	46.022	28.262	57.228	44.478	11.570	84.359	16.249
Normal	15	15	15	15	450	NS-2	4.03	1.124	0.102	7.828	15.824	63.790	30.569	91.270	66.386	7.521	105.220	18.474
Normal	15	15	15	15	150	NS-9	1.91	8.570	0.779	1.080	1.985	3.801	2.260	4.750	2.842	0.900	9.298	2.729
Normal	15	15	15	15	450	NS-9	3.44	8.570	0.779	1.058	1.905	6.549	3.191	10.379	7.848	0.818	11.880	2.547
Normal	15	15	15	15	150	NS-10	1.88	7.740	0.704	2.327	3.090	5.812	3.945	6.657	5.282	1.566	10.876	1.587
Normal	15	15	15	15	450	NS-10	3.49	7.740	0.704	1.361	3.070	10.725	5.212	12.847	10.236	1.316	16.394	1.903
Normal	15	15	15	15	150	NS-11	2.82	4.810	0.437	2.498	6.458	18.209	11.740	20.707	18.690	2.822	24.058	3.987
Normal	15	15	15	15	450	NS-11	3.50	4.810	0.437	2.275	4.955	17.354	9.775	24.616	23.000	2.121	25.048	4.893
Normal	15	15	15	15	150	NS-12	1.28	6.030	0.548	1.094	2.955	3.784	3.746	3.851	3.496	1.049	6.089	17.353
Normal	15	15	15	15	450	NS-12	3.65	6.030	0.548	2.190	4.342	15.838	9.115	20.293	15.780	1.794	26.608	3.700
Normal	15	15	15	15	150	NS-13	1.90	6.640	0.604	3.143	4.752	9.041	6.052	13.419	10.180	1.714	16.471	3.988
Normal	15	15	15	15	450	NS-13	4.04	6.640	0.604	1.861	3.726	15.067	9.803	20.788	19.575	2.139	22.655	4.888
Normal	15	15	15	15	150	NS-14	2.29	8.590	0.781	2.201	3.742	8.578	5.631	10.347	12.358	1.302	13.315	2.766
Normal	15	15	15	15	450	NS-14	3.98	8.590	0.781	1.604	3.248	12.918	7.060	17.275	13.675	1.290	19.985	3.165
Low	5	5	15	15	150	LS-2	1.05	10.060	0.915	0.696	0.764	0.799	0.842	1.332	0.889	0.418	2.562	4.606
Low	5	5	15	15	450	LS-2	0.97	10.060	0.915	0.606	0.735	0.716	0.776	1.284	0.849	0.536	3.221	6.353
Low	5	5	15	15	150	LS-3	0.92	0.950	0.086	7.477	8.924	8.177	9.146	13.525	9.032	5.181	29.590	22.188
Low	5	5	15	15	450	LS-3	1.01	0.950	0.086	5.137	6.443	6.528	7.064	11.253	7.361	4.624	27.404	26.041
Low	5	5	15	15	150	LS-4	2.62	5.160	0.469	2.732	6.764	17.705	10.308	21.430	14.800	3.434	24.831	4.422
Low	5	5	15	15	450	LS-4	2.93	5.160	0.469	3.139	5.729	16.797	9.984	20.717	13.720	2.612	26.795	5.565
Low	5	5	15	15	150	LS-5	2.59	8.990	0.817	0.900	2.107	5.446	3.055	6.517	5.199	0.866	8.282	2.297
Low	5	5	15	15	450	LS-5	2.67	8.990	0.817	0.837	1.456	3.888	2.484	5.098	3.645	0.737	10.808	3.138
Low	5	5	15	15	150	LS-6	3.98	19.620	1.784	0.638	1.281	5.098	2.715	7.032	5.281	0.426	7.178	1.572
Low	5	5	15	15	450	LS-6	4.83	19.620	1.784	0.503	1.001	4.833	2.327	7.376	5.341	0.464	8.443	2.111
Low	5	5	15	15	150	LS-7	5.96	6.835	0.621	1.713	3.990	23.793	10.496	31.672	24.891	1.641	34.254	5.030
Low	5	5	15	15	450	LS-7	3.75	6.835	0.621	2.277	5.178	19.440	10.546	27.476	20.284	1.977	28.710	4.916
Low	5	5	15	15	150	LS-8	3.16	12.390	1.126	0.983	2.904	9.164	5.658	13.116	10.609	1.390	13.543	2.862
Low	5	5	15	15	450	LS-8	4.29	12.390	1.126	1.031	2.859	12.276	7.060	17.360	13.973	1.179	17.442	2.529
Low	5	5	15	15	150	LS-9	2.80	10.468	0.952	1.549	3.633	10.164	6.911	14.049	12.567	1.780	14.332	2.843
Low	5	5	15	15	450	LS-9	4.41	10.468	0.952	1.331	3.456	15.253	8.039	19.959	16.733	1.394	20.831	3.813