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Yang, Bingye; Pu, F; Li, lingling; You, W; Ke, C; Feng, Danqing (2017): Functional analysis of a tyrosinase gene involved in early larval shell biogenesis in Crassostrea angulata and its response to ocean acidification [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.874743, Supplement to: Yang, B et al. (2017): Functional analysis of a tyrosinase gene involved in early larval shell biogenesis in Crassostrea angulata and its response to ocean acidification. Comparative Biochemistry and Physiology Part B: Biochemistry & Molecular Biology, 206, 8-15, https://doi.org/10.1016/j.cbpb.2017.01.006

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Abstract:
The formation of the primary shell is a vital process in marine bivalves. Ocean acidification largely influences shell formation. It has been reported that enzymes involved in phenol oxidation, such as tyrosinase and phenoloxidases, participate in the formation of the periostracum. In the present study, we cloned a tyrosinase gene from Crassostrea angulata named Ca-tyrA1, and its potential function in early larval shell biogenesis was investigated. The Ca-tyrA1 gene has a full-length cDNA of 2430 bp in size, with an open reading frame of 1896 bp in size, which encodes a 631-amino acid protein that includes a 24-amino acid putative signal peptide. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis revealed that Ca-tyrA1 transcription mainly occurs at the trochophore stage, and the Ca-tyrA1 mRNA levels in the 3000 ppm treatment group were significantly upregulated in the early D-veliger larvae. WMISH and electron scanning microscopy analyses showed that the expression of Ca-tyrA1 occurs at the gastrula stage, thereby sustaining the early D-veliger larvae, and the shape of its signal is saddle-like, similar to that observed under an electron scanning microscope. Furthermore, the RNA interference has shown that the treatment group has a higher deformity rate than that of the control, thereby indicating that Ca-tyrA1 participates in the biogenesis of the primary shell. In conclusion, and our results indicate that Ca-tyrA1 plays a vital role in the formation of the larval shell and participates in the response to larval shell damages in Crassostrea angulata that were induced by ocean acidification.
Keyword(s):
Animalia; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea angulata; Gene expression (incl. proteomics); Laboratory experiment; Mollusca; North Pacific; Pelagos; Single species; Temperate; Zooplankton
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2017-04-20.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1TypeTypeFeng, Danqingstudy
2SpeciesSpeciesFeng, Danqing
3Registration number of speciesReg spec noFeng, Danqing
4Uniform resource locator/link to referenceURL refFeng, DanqingWoRMS Aphia ID
5StageStageFeng, Danqing
6TreatmentTreatFeng, Danqing
7Gene expressionGene expressionFeng, Danqingrelative expression level of Ca-tyr3
8Gene expression, standard deviationGene expression std dev±Feng, Danqingrelative expression level of Ca-tyr3
9SalinitySalFeng, Danqing
10Temperature, waterTemp°CFeng, Danqing
11Alkalinity, totalATµmol/kgFeng, Danqing
12Carbon, inorganic, dissolvedDICµmol/kgFeng, Danqing
13Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
14pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
15Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
16Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
17Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
18Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
19Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
20Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
21Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
252 data points

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