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Kottmeier, Dorothee; Rokitta, Sebastian D; Rost, Björn (2017): H+-driven increase in CO2 uptake and decrease in HCO3- uptake explain coccolithophores' acclimation responses to ocean acidification. PANGAEA, https://doi.org/10.1594/PANGAEA.873376, Supplement to: Kottmeier, D et al. (2016): H+-driven increase in CO2 uptake and decrease in HCO3- uptake explain coccolithophores' acclimation responses to ocean acidification. Limnology and Oceanography, 61(6), 2045-2057, https://doi.org/10.1002/lno.10352

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Abstract:
Recent ocean acidification (OA) studies revealed that seawater [H+] rather than [CO2] or [ inline image] regulate short-term responses in carbon fluxes of Emiliania huxleyi. Here, we investigated whether acclimation to altered carbonate chemistry modulates this regulation pattern and how the carbon supply for calcification is affected by carbonate chemistry. We acclimated E. huxleyi to present-day (ambient [CO2], [ inline image], and pH) and OA conditions (high [CO2], ambient [ inline image], low pH). To differentiate between the CO2 and pH/H+ effects, we also acclimated cells to carbonation (high [CO2] and [ inline image], ambient pH) and acidification (ambient [CO2], low [ inline image], and pH). Under these conditions, growth, production of particulate inorganic and organic carbon, as well as carbon and oxygen fluxes were measured. Under carbonation, photosynthesis and calcification were stimulated due to additional inline image uptake, whereas growth was unaffected. Such stimulatory effects are not apparent after short-term carbonation, indicating that cells adjusted their carbon acquisition during acclimation. Being driven by [ inline image], these regulations can, however, not explain typical OA effects. Under acidification and OA, photosynthesis stayed constant, whereas calcification and growth decreased. Similar to the short-term responses toward high [H+], CO2 uptake significantly increased, but inline image uptake decreased. This antagonistic regulation in CO2 and inline image uptake can explain why photosynthesis, being able to use CO2 and inline image, often benefits from OA, whereas calcification, being mostly dependent on inline image, often decreases. We identified H+ as prime driver of coccolithophores' acclimation responses toward OA. Acidified conditions seem to put metabolic burdens on the cells that result in decreased growth.
Keyword(s):
Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloise; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2017-03-10.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1TypeTypeKottmeier, Dorotheestudy
2SpeciesSpeciesKottmeier, Dorothee
3Registration number of speciesReg spec noKottmeier, Dorothee
4Uniform resource locator/link to referenceURL refKottmeier, DorotheeWoRMS Aphia ID
5TreatmentTreatKottmeier, Dorothee
6Growth rateµ1/dayKottmeier, Dorothee
7Growth rate, standard deviationµ std dev±Kottmeier, Dorothee
8Carbon, organic, particulate, per cellPOCpg/#Kottmeier, Dorothee
9Carbon, organic, particulate, standard deviationPOC std dev±Kottmeier, Dorothee
10Production of particulate organic carbon per cellPOC prodpg/#/dayKottmeier, Dorothee
11Particulate organic carbon, production, standard deviationPOC prod std dev±Kottmeier, Dorothee
12Carbon, inorganic, particulate, per cellPIC/cellpg/#Kottmeier, Dorothee
13Carbon, inorganic, particulate, per volume, standard deviationPIC std dev±Kottmeier, Dorothee
14Particulate inorganic carbon production per cellPIC prodpg/#/dayKottmeier, Dorothee
15Particulate inorganic carbon, production, standard deviationPIC prod std dev±Kottmeier, Dorothee
16Particulate inorganic carbon/particulate organic carbon ratioPIC/POCKottmeier, Dorothee
17Particulate inorganic carbon/particulate organic carbon ratio, standard deviationPIC/POC ratio std dev±Kottmeier, Dorothee
18Particulate inorganic carbon/particulate organic carbon ratioPIC/POCKottmeier, Dorothee
19Particulate inorganic carbon/particulate organic carbon ratio, standard deviationPIC/POC ratio std dev±Kottmeier, Dorothee
20Chlorophyll a per cellChl apg/#Kottmeier, Dorothee
21Chlorophyll a, standard deviationChl a std dev±Kottmeier, Dorothee
22Chlorophyll a/particulate organic carbon ratioChl a/POCKottmeier, Dorothee
23Chlorophyll a/particulate organic carbon ratio, standard deviationChl a/POC std dev±Kottmeier, Dorothee
24Particulate organic nitrogen per cellPON cellpg/#Kottmeier, Dorothee
25Particulate organic nitrogen per cell, standard deviationPON cell std dev±Kottmeier, Dorothee
26Carbon, organic, particulate/Nitrogen, organic, particulate ratioPOC/PONKottmeier, Dorothee
27Carbon, organic, particulate/Nitrogen, organic, particulate ratio, standard deviationPOC/PON std dev±Kottmeier, Dorothee
28Net photosynthesis rate, oxygen, per chlorophyll aPN O2µmol/mg/hKottmeier, Dorothee
29Net photosynthesis rate, standard deviationPN std dev±Kottmeier, Dorothee
30Growth rateµ1/dayKottmeier, Dorotheeduring cellular flux measurements
31Growth rate, standard deviationµ std dev±Kottmeier, Dorotheeduring cellular flux measurements
32Carbon dioxide uptake rate in chlorophyllCO2 upt rate/Chlµmol/mg/hKottmeier, Dorotheephotosynthetic
33Carbon dioxide uptake in chlorophyll, standard deviationCO2 upt/Chl std dev±Kottmeier, Dorotheephotosynthetic
34Bicarbonate uptake rate in chlorophyllHCO3 upt rate/Chlµmol/mg/hKottmeier, Dorotheephotosynthetic
35Bicarbonate uptake in chlorophyll, standard deviationHCO3 upt/Chl std dev±Kottmeier, Dorotheephotosynthetic
36Carbon dioxide usage fractionfCO2Kottmeier, Dorotheeratio of photosynthetic CO2 uptake to overall photosynthetic Ci uptake
37Carbon dioxide usage fraction, standard deviationfCO2 std dev±Kottmeier, Dorotheeratio of photosynthetic CO2 uptake to overall photosynthetic Ci uptake
38Calcification rate of calcium carbonateCalc rate CaCO3µmol/g/hKottmeier, Dorothee
39Calcification rate, standard deviationCalc rate std dev±Kottmeier, Dorothee
40Carbon dioxide uptake rate in chlorophyllCO2 upt rate/Chlµmol/mg/hKottmeier, Dorotheefor calcification
41Carbon dioxide uptake in chlorophyll, standard deviationCO2 upt/Chl std dev±Kottmeier, Dorotheefor calcification
42Bicarbonate uptake rate in chlorophyllHCO3 upt rate/Chlµmol/mg/hKottmeier, Dorotheefor calcification
43Bicarbonate uptake in chlorophyll, standard deviationHCO3 upt/Chl std dev±Kottmeier, Dorotheefor calcification
44Carbon dioxide uptake rate in chlorophyllCO2 upt rate/Chlµmol/mg/hKottmeier, Dorotheetotal
45Carbon dioxide uptake in chlorophyll, standard deviationCO2 upt/Chl std dev±Kottmeier, Dorotheetotal
46Bicarbonate uptake rate in chlorophyllHCO3 upt rate/Chlµmol/mg/hKottmeier, Dorotheetotal
47Bicarbonate uptake in chlorophyll, standard deviationHCO3 upt/Chl std dev±Kottmeier, Dorotheetotal
48RatioRatioKottmeier, DorotheeHCO3- uptake for calcification to HCO3- uptake for photosynthesis
49Ratio, standard deviationRatio std dev±Kottmeier, DorotheeHCO3- uptake for calcification to HCO3- uptake for photosynthesis
50Respiration rate, oxygenResp O2µmol/mg/hKottmeier, Dorotheeper Chl a
51Respiration rate, oxygen, standard deviationResp O2 std dev±Kottmeier, Dorotheeper Chl a
52RatioRatioKottmeier, Dorotheephotosynthesis rate/respiration
53Ratio, standard deviationRatio std dev±Kottmeier, Dorotheephotosynthesis rate/respiration
54Temperature, waterTemp°CKottmeier, Dorothee
55SalinitySalKottmeier, Dorothee
56Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmKottmeier, Dorotheecell-free media
57Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Kottmeier, Dorotheecell-free media
58pHpHKottmeier, DorotheeNBS scale, cell-free media
59pH, standard deviationpH std dev±Kottmeier, DorotheeNBS scale, cell-free media
60Hydrogen ion concentrationH+µmol/kgKottmeier, Dorotheecell-free media
61Hydrogen ion concentration, standard deviationH+ std dev±Kottmeier, Dorotheecell-free media
62Alkalinity, totalATµmol/kgKottmeier, Dorotheecell-free media
63Alkalinity, total, standard deviationAT std dev±Kottmeier, Dorotheecell-free media
64Carbon, inorganic, dissolvedDICµmol/kgKottmeier, Dorotheecell-free media
65Carbon, inorganic, dissolved, standard deviationDIC std dev±Kottmeier, Dorotheecell-free media
66Carbon dioxideCO2µmol/kgKottmeier, Dorotheecell-free media
67Carbon dioxide, standard deviationCO2 std dev±Kottmeier, Dorotheecell-free media
68Bicarbonate ion[HCO3]-µmol/kgKottmeier, Dorotheecell-free media
69Bicarbonate ion, standard deviation[HCO3]- std dev±Kottmeier, Dorotheecell-free media
70Carbonate ion[CO3]2-µmol/kgKottmeier, Dorotheecell-free media
71Carbonate ion, standard deviation[CO3]2- std dev±Kottmeier, Dorotheecell-free media
72Calcite saturation stateOmega CalKottmeier, Dorotheecell-free media
73Calcite saturation state, standard deviationOmega Cal std dev±Kottmeier, Dorotheecell-free media
74Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmKottmeier, Dorotheeat the time of harvesting
75Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Kottmeier, Dorotheeat the time of harvesting
76pHpHKottmeier, DorotheeNBS scale, at the time of harvesting
77pH, standard deviationpH std dev±Kottmeier, DorotheeNBS scale, at the time of harvesting
78Hydrogen ion concentrationH+µmol/kgKottmeier, Dorotheeat the time of harvesting
79Hydrogen ion concentration, standard deviationH+ std dev±Kottmeier, Dorotheeat the time of harvesting
80Alkalinity, totalATµmol/kgKottmeier, Dorotheeat the time of harvesting
81Alkalinity, total, standard deviationAT std dev±Kottmeier, Dorotheeat the time of harvesting
82Carbon, inorganic, dissolvedDICµmol/kgKottmeier, Dorotheeat the time of harvesting
83Carbon, inorganic, dissolved, standard deviationDIC std dev±Kottmeier, Dorotheeat the time of harvesting
84Carbon dioxideCO2µmol/kgKottmeier, Dorotheeat the time of harvesting
85Carbon dioxide, standard deviationCO2 std dev±Kottmeier, Dorotheeat the time of harvesting
86Bicarbonate ion[HCO3]-µmol/kgKottmeier, Dorotheeat the time of harvesting
87Bicarbonate ion, standard deviation[HCO3]- std dev±Kottmeier, Dorotheeat the time of harvesting
88Carbonate ion[CO3]2-µmol/kgKottmeier, Dorotheeat the time of harvesting
89Carbonate ion, standard deviation[CO3]2- std dev±Kottmeier, Dorotheeat the time of harvesting
90Calcite saturation stateOmega CalKottmeier, Dorotheeat the time of harvesting
91Calcite saturation state, standard deviationOmega Cal std dev±Kottmeier, Dorotheeat the time of harvesting
92Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmKottmeier, Dorotheeduring cellular flux measurements
93Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Kottmeier, Dorotheeduring cellular flux measurements
94pHpHKottmeier, DorotheeNBS scale, during cellular flux measurements
95pH, standard deviationpH std dev±Kottmeier, DorotheeNBS scale, during cellular flux measurements
96Hydrogen ion concentrationH+µmol/kgKottmeier, Dorotheeduring cellular flux measurements
97Hydrogen ion concentration, standard deviationH+ std dev±Kottmeier, Dorotheeduring cellular flux measurements
98Carbon, inorganic, dissolvedDICµmol/kgKottmeier, Dorotheeduring cellular flux measurements
99Carbon, inorganic, dissolved, standard deviationDIC std dev±Kottmeier, Dorotheeduring cellular flux measurements
100Carbon dioxideCO2µmol/kgKottmeier, Dorotheeduring cellular flux measurements
101Carbon dioxide, standard deviationCO2 std dev±Kottmeier, Dorotheeduring cellular flux measurements
102Bicarbonate ion[HCO3]-µmol/kgKottmeier, Dorotheeduring cellular flux measurements
103Bicarbonate ion, standard deviation[HCO3]- std dev±Kottmeier, Dorotheeduring cellular flux measurements
104Carbonate ion[CO3]2-µmol/kgKottmeier, Dorotheeduring cellular flux measurements
105Carbonate ion, standard deviation[CO3]2- std dev±Kottmeier, Dorotheeduring cellular flux measurements
106Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
107pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale, cell-free media
108Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)cell-free media
109Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)cell-free media
110Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)cell-free media
111Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)cell-free media
112Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)cell-free media
113Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)cell-free media
114Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)cell-free media
115Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)cell-free media
116pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale, at the time of harvesting
117Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)at the time of harvesting
118Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)at the time of harvesting
119Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)at the time of harvesting
120Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)at the time of harvesting
121Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)at the time of harvesting
122Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)at the time of harvesting
123Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)at the time of harvesting
124Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)at the time of harvesting
Size:
495 data points

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