Kottmeier, Dorothee; Rokitta, Sebastian D; Rost, Björn (2016): H+-driven increase in CO2 uptake and decrease in HCO3- uptake explain coccolithophores' acclimation responses to ocean acidification [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.873376, Supplement to: Kottmeier, D et al. (2016): H+-driven increase in CO2 uptake and decrease in HCO3- uptake explain coccolithophores' acclimation responses to ocean acidification. Limnology and Oceanography, 61(6), 2045-2057, https://doi.org/10.1002/lno.10352
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Abstract:
Recent ocean acidification (OA) studies revealed that seawater [H+] rather than [CO2] or [ inline image] regulate short-term responses in carbon fluxes of Emiliania huxleyi. Here, we investigated whether acclimation to altered carbonate chemistry modulates this regulation pattern and how the carbon supply for calcification is affected by carbonate chemistry. We acclimated E. huxleyi to present-day (ambient [CO2], [ inline image], and pH) and OA conditions (high [CO2], ambient [ inline image], low pH). To differentiate between the CO2 and pH/H+ effects, we also acclimated cells to carbonation (high [CO2] and [ inline image], ambient pH) and acidification (ambient [CO2], low [ inline image], and pH). Under these conditions, growth, production of particulate inorganic and organic carbon, as well as carbon and oxygen fluxes were measured. Under carbonation, photosynthesis and calcification were stimulated due to additional inline image uptake, whereas growth was unaffected. Such stimulatory effects are not apparent after short-term carbonation, indicating that cells adjusted their carbon acquisition during acclimation. Being driven by [ inline image], these regulations can, however, not explain typical OA effects. Under acidification and OA, photosynthesis stayed constant, whereas calcification and growth decreased. Similar to the short-term responses toward high [H+], CO2 uptake significantly increased, but inline image uptake decreased. This antagonistic regulation in CO2 and inline image uptake can explain why photosynthesis, being able to use CO2 and inline image, often benefits from OA, whereas calcification, being mostly dependent on inline image, often decreases. We identified H+ as prime driver of coccolithophores' acclimation responses toward OA. Acidified conditions seem to put metabolic burdens on the cells that result in decreased growth.
Keyword(s):
Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb
Project(s):
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2017-03-10.
Parameter(s):
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
495 data points
Data
1 Type (study) | 2 Species | 3 Reg spec no | 4 URL ref (WoRMS Aphia ID) | 5 Treat | 6 µ [1/day] | 7 µ std dev [±] | 8 POC/cell [pg/#] | 9 POC std dev [±] | 10 POC prod/cell [pg/#/day] | 11 POC prod std dev [±] | 12 PIC/cell [pg/#] | 13 PIC std dev [±] | 14 PIC prod/cell [pg/#/day] | 15 PIC prod std dev [±] | 16 PIC/POC | 17 PIC/POC ratio std dev [±] | 18 PIC/POC | 19 PIC/POC ratio std dev [±] | 20 Chl a/cell [pg/#] | 21 Chl a std dev [±] | 22 Chl a/POC | 23 Chl a/POC std dev [±] | 24 PON/cell [pg/#] | 25 PON/cell std dev [±] | 26 POC/PON | 27 POC/PON std dev [±] | 28 PN O2 [µmol/mg/h] | 29 PN std dev [±] | 30 µ [1/day] (during cellular flux measurem...) | 31 µ std dev [±] (during cellular flux measurem...) | 32 CO2 upt rate/Chl [µmol/mg/h] (photosynthetic) | 33 CO2 upt/Chl std dev [±] (photosynthetic) | 34 HCO3 upt rate/Chl [µmol/mg/h] (photosynthetic) | 35 HCO3 upt/Chl std dev [±] (photosynthetic) | 36 fCO2 (ratio of photosynthetic CO2 u...) | 37 fCO2 std dev [±] (ratio of photosynthetic CO2 u...) | 38 Calc rate CaCO3 [µmol/g/h] | 39 Calc rate std dev [±] | 40 CO2 upt rate/Chl [µmol/mg/h] (for calcification) | 41 CO2 upt/Chl std dev [±] (for calcification) | 42 HCO3 upt rate/Chl [µmol/mg/h] (for calcification) | 43 HCO3 upt/Chl std dev [±] (for calcification) | 44 CO2 upt rate/Chl [µmol/mg/h] (total) | 45 CO2 upt/Chl std dev [±] (total) | 46 HCO3 upt rate/Chl [µmol/mg/h] (total) | 47 HCO3 upt/Chl std dev [±] (total) | 48 Ratio (HCO3- uptake for calcificatio...) | 49 Ratio std dev [±] (HCO3- uptake for calcificatio...) | 50 Resp O2 [µmol/mg/h] (per Chl a) | 51 Resp O2 std dev [±] (per Chl a) | 52 Ratio (photosynthesis rate/respiration) | 53 Ratio std dev [±] (photosynthesis rate/respiration) | 54 Temp [°C] | 55 Sal | 56 pCO2water_SST_wet [µatm] (cell-free media) | 57 pCO2 std dev [±] (cell-free media) | 58 pH (NBS scale, cell-free media) | 59 pH std dev [±] (NBS scale, cell-free media) | 60 H+ [µmol/kg] (cell-free media) | 61 H+ std dev [±] (cell-free media) | 62 AT [µmol/kg] (cell-free media) | 63 AT std dev [±] (cell-free media) | 64 DIC [µmol/kg] (cell-free media) | 65 DIC std dev [±] (cell-free media) | 66 CO2 [µmol/kg] (cell-free media) | 67 CO2 std dev [±] (cell-free media) | 68 [HCO3]- [µmol/kg] (cell-free media) | 69 [HCO3]- std dev [±] (cell-free media) | 70 [CO3]2- [µmol/kg] (cell-free media) | 71 [CO3]2- std dev [±] (cell-free media) | 72 Omega Cal (cell-free media) | 73 Omega Cal std dev [±] (cell-free media) | 74 pCO2water_SST_wet [µatm] (at the time of harvesting) | 75 pCO2 std dev [±] (at the time of harvesting) | 76 pH (NBS scale, at the time of har...) | 77 pH std dev [±] (NBS scale, at the time of har...) | 78 H+ [µmol/kg] (at the time of harvesting) | 79 H+ std dev [±] (at the time of harvesting) | 80 AT [µmol/kg] (at the time of harvesting) | 81 AT std dev [±] (at the time of harvesting) | 82 DIC [µmol/kg] (at the time of harvesting) | 83 DIC std dev [±] (at the time of harvesting) | 84 CO2 [µmol/kg] (at the time of harvesting) | 85 CO2 std dev [±] (at the time of harvesting) | 86 [HCO3]- [µmol/kg] (at the time of harvesting) | 87 [HCO3]- std dev [±] (at the time of harvesting) | 88 [CO3]2- [µmol/kg] (at the time of harvesting) | 89 [CO3]2- std dev [±] (at the time of harvesting) | 90 Omega Cal (at the time of harvesting) | 91 Omega Cal std dev [±] (at the time of harvesting) | 92 pCO2water_SST_wet [µatm] (during cellular flux measurem...) | 93 pCO2 std dev [±] (during cellular flux measurem...) | 94 pH (NBS scale, during cellular fl...) | 95 pH std dev [±] (NBS scale, during cellular fl...) | 96 H+ [µmol/kg] (during cellular flux measurem...) | 97 H+ std dev [±] (during cellular flux measurem...) | 98 DIC [µmol/kg] (during cellular flux measurem...) | 99 DIC std dev [±] (during cellular flux measurem...) | 100 CO2 [µmol/kg] (during cellular flux measurem...) | 101 CO2 std dev [±] (during cellular flux measurem...) | 102 [HCO3]- [µmol/kg] (during cellular flux measurem...) | 103 [HCO3]- std dev [±] (during cellular flux measurem...) | 104 [CO3]2- [µmol/kg] (during cellular flux measurem...) | 105 [CO3]2- std dev [±] (during cellular flux measurem...) | 106 CSC flag | 107 pH (total scale, cell-free media) | 108 CO2 [µmol/kg] (cell-free media) | 109 fCO2water_SST_wet [µatm] (cell-free media) | 110 pCO2water_SST_wet [µatm] (cell-free media) | 111 [HCO3]- [µmol/kg] (cell-free media) | 112 [CO3]2- [µmol/kg] (cell-free media) | 113 DIC [µmol/kg] (cell-free media) | 114 Omega Arg (cell-free media) | 115 Omega Cal (cell-free media) | 116 pH (total scale, at the time of h...) | 117 CO2 [µmol/kg] (at the time of harvesting) | 118 fCO2water_SST_wet [µatm] (at the time of harvesting) | 119 pCO2water_SST_wet [µatm] (at the time of harvesting) | 120 [HCO3]- [µmol/kg] (at the time of harvesting) | 121 [CO3]2- [µmol/kg] (at the time of harvesting) | 122 DIC [µmol/kg] (at the time of harvesting) | 123 Omega Arg (at the time of harvesting) | 124 Omega Cal (at the time of harvesting) |
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laboratory | Emiliania huxleyi (phytoplankton) | 816368 | marinespecies.org | present-day | 1.16 | 0.01 | 8.9 | 0.6 | 10.1 | 0.7 | 10.0 | 0.3 | 11.3 | 0.3 | 1.12 | 0.08 | 0.87 | 0.02 | 0.13 | 0.01 | 0.014 | 0.001 | 1.5 | 0.1 | 6.9 | 0.2 | 284 | 41 | 1.06 | 0.15 | 15 | 11 | 244 | 34 | 0.06 | 0.03 | 237000 | 34000 | 47 | 7 | 189 | 28 | 57 | 15 | 448 | 32 | 0.77 | 0.03 | 100 | 27 | 3.0 | 0.8 | 15 | 31 | 403 | 4 | 8.13 | 0.00 | 0.0099 | 0.0001 | 2341 | 4 | 2129 | 4 | 15 | 0 | 1961 | 5 | 153 | 1 | 3.7 | 0.0 | 384 | 17 | 8.14 | 0.01 | 9.9 | 0.1 | 2280 | 19 | 2068 | 23 | 15 | 1 | 1903 | 25 | 151 | 3 | 3.7 | 0.1 | 486 | 17 | 8.15 | 0.01 | 9.3 | 0.2 | 2323 | 180 | 21 | 3 | 2252 | 264 | 160 | 5 | 26 | 8.01 | 17.61 | 459.63 | 461.30 | 1996.90 | 138.09 | 2152.60 | 2.15 | 3.38 | 8.02 | 16.69 | 435.66 | 437.24 | 1936.78 | 137.05 | 2090.52 | 2.13 | 3.35 |
laboratory | Emiliania huxleyi (phytoplankton) | 816368 | marinespecies.org | carbonation | 1.15 | 0.04 | 10.2 | 0.3 | 11.4 | 0.4 | 14.0 | 1.0 | 15.7 | 1.1 | 1.37 | 0.08 | 1.15 | 0.03 | 0.13 | 0.01 | 0.013 | 0.001 | 1.7 | 0.2 | 6.8 | 0.4 | 371 | 11 | 1.22 | 0.04 | -30 | 17 | 368 | 27 | -0.09 | 0.05 | 390000 | 18000 | 78 | 4 | 312 | 15 | 48 | 14 | 679 | 41 | 0.85 | 0.03 | 107 | 12 | 3.5 | 0.4 | 15 | 31 | 868 | 109 | 8.16 | 0.02 | 0.0095 | 0.0001 | 5317 | 560 | 4899 | 529 | 33 | 4 | 4493 | 489 | 373 | 38 | 9.1 | 0.9 | 805 | 84 | 8.18 | 0.00 | 8.9 | 0.3 | 5223 | 527 | 4791 | 491 | 31 | 3 | 4379 | 450 | 382 | 39 | 9.3 | 0.9 | 883 | 13 | 8.18 | 0.02 | 8.5 | 0.4 | 4648 | 69 | 36 | 4263 | 64 | 333 | 5 | 26 | 8.04 | 37.57 | 980.73 | 984.29 | 4568.04 | 338.67 | 4944.28 | 5.27 | 8.28 | 8.06 | 35.00 | 913.76 | 917.08 | 4456.82 | 346.00 | 4837.83 | 5.39 | 8.46 | |
laboratory | Emiliania huxleyi (phytoplankton) | 816368 | marinespecies.org | acidification | 1.02 | 0.03 | 9.7 | 0.7 | 9.7 | 0.7 | 9.5 | 0.5 | 9.5 | 0.5 | 0.98 | 0.03 | 0.82 | 0.06 | 0.12 | 0.01 | 0.013 | 0.004 | 1.8 | 0.1 | 6.9 | 0.3 | 268 | 41 | 0.88 | 0.13 | 93 | 21 | 151 | 23 | 0.38 | 0.05 | 202000 | 41000 | 40 | 8 | 161 | 34 | 134 | 29 | 312 | 54 | 1.07 | 0.15 | 77 | 16 | 3.6 | 1.1 | 15 | 31 | 418 | 12 | 7.83 | 0.00 | 0.0201 | 0.0002 | 1122 | 19 | 1056 | 19 | 16 | 0 | 1002 | 19 | 38 | 0 | 0.9 | 15.0 | 410 | 51 | 7.83 | 0.04 | 19.0 | 1.1 | 1119 | 31 | 1052 | 37 | 16 | 2 | 997 | 37 | 39 | 2 | 1.0 | 0.1 | 405 | 12 | 7.88 | 0.02 | 17.3 | 0.8 | 1037 | 31 | 17 | 1 | 980 | 30 | 38 | 1 | 26 | 7.71 | 17.82 | 465.20 | 466.88 | 1012.05 | 35.05 | 1064.92 | 0.55 | 0.86 | 7.71 | 17.77 | 463.92 | 465.60 | 1009.25 | 34.95 | 1061.97 | 0.54 | 0.85 |
laboratory | Emiliania huxleyi (phytoplankton) | 816368 | marinespecies.org | ocean acidification | 1.01 | 0.02 | 8.9 | 0.2 | 8.8 | 0.2 | 9.5 | 0.3 | 9.2 | 0.3 | 1.05 | 0.03 | 0.88 | 0.02 | 0.12 | 0.01 | 0.014 | 0.002 | 1.6 | 0.1 | 6.6 | 0.2 | 295 | 38 | 1.07 | 0.12 | 105 | 20 | 163 | 53 | 0.40 | 0.13 | 222000 | 32000 | 50 | 2 | 178 | 25 | 156 | 20 | 352 | 80 | 1.22 | 0.28 | 85 | 4 | 3.5 | 0.4 | 15 | 31 | 998 | 15 | 7.78 | 0.01 | 0.0225 | 0.0002 | 2312 | 2 | 2238 | 2 | 38 | 1 | 2127 | 2 | 73 | 1 | 1.8 | 0.0 | 964 | 8 | 7.79 | 0.00 | 22.1 | 0.2 | 2287 | 4 | 2211 | 4 | 37 | 0 | 2100 | 4 | 73 | 1 | 1.8 | 0.0 | 942 | 28 | 7.87 | 0.02 | 17.8 | 0.7 | 2357 | 70 | 38 | 1 | 2230 | 67 | 85 | 3 | 26 | 7.66 | 42.32 | 1104.88 | 1108.89 | 2143.15 | 66.17 | 2251.64 | 1.03 | 1.62 | 7.67 | 40.84 | 1066.18 | 1070.05 | 2116.13 | 66.85 | 2223.83 | 1.04 | 1.64 |