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Sordo, Laura; Santos, Rui; Reis, Joao; Shulika, Alona; Silva, João (2016): A direct CO2 control system for ocean acidification experiments: testing effects on the coralline red algae Phymatolithon lusitanicum [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.867506, Supplement to: Sordo, L et al. (2016): A direct CO2 control system for ocean acidification experiments: testing effects on the coralline red algae Phymatolithon lusitanicum. PeerJ, 4, e2503, https://doi.org/10.7717/peerj.2503

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Published: 2016-11-04 • DOI registered: 2016-12-02

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Abstract:
Most ocean acidification (OA) experimental systems rely on pH as an indirect way to control CO2. However, accurate pH measurements are difficult to obtain and shifts in temperature and/or salinity alter the relationship between pH and pCO2. Here we describe a system in which the target pCO2 is controlled via direct analysis of pCO2 in seawater. This direct type of control accommodates potential temperature and salinity shifts, as the target variable is directly measured instead of being estimated. Water in a header tank is permanently re-circulated through an air-water equilibrator. The equilibrated air is then routed to an infrared gas analyzer (IRGA) that measures pCO2 and conveys this value to a Proportional-Integral-Derivative (PID) controller. The controller commands a solenoid valve that opens and closes the CO2 flush that is bubbled into the header tank. This low-cost control system allows the maintenance of stabilized levels of pCO2 for extended periods of time ensuring accurate experimental conditions. This system was used to study the long term effect of OA on the coralline red algae Phymatolithon lusitanicum. We found that after 11 months of high CO2 exposure, photosynthesis increased with CO2 as opposed to respiration, which was positively affected by temperature. Results showed that this system is adequate to run long-term OA experiments and can be easily adapted to test other relevant variables simultaneously with CO2, such as temperature, irradiance and nutrients.
Keyword(s):
Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Macroalgae; North Atlantic; Phymatolithon lusitanicum; Plantae; Primary production/Photosynthesis; Respiration; Rhodophyta; Single species; Temperate; Temperature
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.8. https://cran.r-project.org/package=seacarb
Project(s):
Ocean Acidification International Coordination Centre (OA-ICC)
Coverage:
Latitude: 37.183510 * Longitude: -8.316680
Event(s):
Armacao_de_Pera * Latitude: 37.183510 * Longitude: -8.316680 * Method/Device: Experiment (EXP)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-11-03.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1TypeTypeSordo, Laurastudy
2SpeciesSpeciesSordo, Laura
3Registration number of speciesReg spec noSordo, Laura
4Uniform resource locator/link to referenceURL refSordo, LauraWoRMS Aphia ID
5Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmSordo, Lauratreatment
6IrradianceEµmol/m2/sSordo, Laura
7Net photosynthesis rate, oxygenPN O2µmol/m2/sSordo, Laura
8Net photosynthesis rate, oxygenPN O2µmol/g/sSordo, Laura
9Temperature, waterTemp°CSordo, Lauratreatment
10Respiration rate, oxygenResp O2µmol/m2/sSordo, Laura
11Respiration rate, oxygen, standard errorResp O2 std e±Sordo, Laura
12Alkalinity, totalATµmol/kgSordo, LauraPotentiometric titration
13Alkalinity, total, standard errorAT std e±Sordo, LauraPotentiometric titration
14SalinitySalSordo, Laura
15Salinity, standard errorSal std e±Sordo, Laura
16Temperature, waterTemp°CSordo, Laura
17Temperature, water, standard errorT std e±Sordo, Laura
18pH, NBS scalepH NBSSordo, LauraPotentiometricNBS scale
19pH, standard errorpH std e±Sordo, LauraPotentiometricNBS scale
20Carbon, inorganic, dissolvedDICµmol/kgSordo, LauraCalculated using CO2SYS
21Carbon, inorganic, dissolved, standard errorDIC std e±Sordo, LauraCalculated using CO2SYS
22Aragonite saturation stateOmega ArgSordo, LauraCalculated using CO2SYS
23Aragonite saturation state, standard errorOmega Arg std e±Sordo, LauraCalculated using CO2SYS
24Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmSordo, LauraCalculated using CO2SYS
25Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard errorpCO2water_SST_wet std e±Sordo, LauraCalculated using CO2SYS
26Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
27pH, total scalepHTYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
28Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
29Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
30Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
31Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
32Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
33Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
34Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
35Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
9073 data points

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