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Hoffmann, Katy; Hassenrück, Christiane; Salman-Carvalho, Verena; Holtappels, Moritz; Bienhold, Christina (2016): Response of Arctic benthic bacterial deep-sea communities to different detritus composition during an ex-situ high pressure experiment. PANGAEA,, Supplement to: Hoffmann, K et al. (2017): Response of bacterial communities to different detritus compositions in Arctic deep-sea sediments. Frontiers in Microbiology, 8, 266,

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In a multidisciplinary ex situ experiment, benthic bacterial deep-sea communities from 2,500 m water depth at the Long-Term Ecological Research Observatory HAUSGARTEN (stationPS93/050-5 and 6), were retrieved using a TV-guided multiple corer. Surface sediments (0 - 2 cm) of 16 cores were mixed with sterile filtered deep-sea water to a final sediment dilution of 3.5 fold. The slurries were split and supplemented with five different types of habitat-related detritus: chitin, as the most abundant biopolymer in the oceans, and four different naturally occurring Arctic algae species, i.e. Thalassiosira weissflogii, Emiliania huxleyi, Bacillaria sp. and Melosira arctica. Incubations were performed in five replicates, at in situ temperature and at atmospheric pressure, as well as at in situ pressure of 250 atm. At the start of the incubation and after 23 days, changes in key community functions, i.e. extracellular enzymatic activity, oxygen respiration and secondary production of biomass (bacterial cell numbers and biomass), were assessed along with changes in the bacterial community composition based on 16S rRNA gene and 16S rRNA Illumina sequencing. In summary, differences in community structure and in the uptake and remineralization of carbon in the different treatments suggest an effect of organic matter quality on bacterial diversity as well as on carbon turnover at the seafloor.
Related to:
Hoffmann, Katy; Hassenrück, Christiane; Salman-Carvalho, Verena; Holtappels, Moritz; Bienhold, Christina (2017): Amplicon sequencing of 16S rRNA of bacterial communities from deep-sea surface sediments in pressure and food source experiments. European Nucleotide Archive (ENA), insdc:PRJEB17614
Soltwedel, Thomas (2015): The Expedition PS93.2 of the Research Vessel POLARSTERN to the Fram Strait in 2015. Berichte zur Polar- und Meeresforschung = Reports on Polar and Marine Research, 693, 68 pp,
Latitude: 79.065100 * Longitude: 4.181000
Date/Time Start: 2015-07-26T00:00:00 * Date/Time End: 2015-07-26T00:00:00
Minimum DEPTH, sediment/rock: 0.01 m * Maximum DEPTH, sediment/rock: 0.01 m
PS93/050-5/6 (HG_IV) * Latitude: 79.065100 * Longitude: 4.181000 * Date/Time: 2015-07-26T00:00:00 * Elevation: -2465.5 m * Location: North Greenland Sea * Campaign: PS93.2 (ARK-XXIX/2.2) * Basis: Polarstern * Method/Device: Multicorer with television (TVMUC) * Comment: Averaged Event from PS93/050-5 and PS93/050-6
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Station labelStationHoffmann, Katy
2Depth, top/minDepth topmHoffmann, Katy
3Depth, bottom/maxDepth botmHoffmann, Katy
4DEPTH, sediment/rockDepthmHoffmann, KatyGeocode
5Sample typeSamp typeHoffmann, Katy
6TreatmentTreatHoffmann, Katyadded to sediment slurry
7Incubation durationInc durdaysHoffmann, Katy
8PressurePbarHoffmann, Katy
9ReplicatesRepl#Hoffmann, Katybiological
10Prokaryotes, abundance as single cellsProk cell abund#/cm3Hoffmann, Katy
11Cell counts, standard deviationCell count std dev±Hoffmann, Katy
12beta-glucosidase activityMUF-bgluµmol/l/hHoffmann, Katy
13Chitobiase activityChitobiase activityµmol/l/hHoffmann, Katy
14OxygenO2µmol/lHoffmann, Katy
15Respiration rate, oxygen, sedimentResp O2 sedµmol/cm3/dayHoffmann, Katyoriginal unit in 23 days
16Accession number, geneticsAccess no genHoffmann, KatyMetagenomic, sequence file
17Accession number, geneticsAccess no genHoffmann, KatyMetatranscriptomic, squence file
1540 data points

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