Citation:

fmicb.2017.00266

Always quote citation above when using data! You can download the citation in several formats below.

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Abstract:

In a multidisciplinary ex situ experiment, benthic bacterial deep-sea communities from 2,500 m water depth at the Long-Term Ecological Research Observatory HAUSGARTEN (stationPS93/050-5 and 6), were retrieved using a TV-guided multiple corer. Surface sediments (0 - 2 cm) of 16 cores were mixed with sterile filtered deep-sea water to a final sediment dilution of 3.5 fold. The slurries were split and supplemented with five different types of habitat-related detritus: chitin, as the most abundant biopolymer in the oceans, and four different naturally occurring Arctic algae species, i.e. Thalassiosira weissflogii, Emiliania huxleyi, Bacillaria sp. and Melosira arctica. Incubations were performed in five replicates, at in situ temperature and at atmospheric pressure, as well as at in situ pressure of 250 atm. At the start of the incubation and after 23 days, changes in key community functions, i.e. extracellular enzymatic activity, oxygen respiration and secondary production of biomass (bacterial cell numbers and biomass), were assessed along with changes in the bacterial community composition based on 16S rRNA gene and 16S rRNA Illumina sequencing. In summary, differences in community structure and in the uptake and remineralization of carbon in the different treatments suggest an effect of organic matter quality on bacterial diversity as well as on carbon turnover at the seafloor.

Related to:

Hoffmann, Katy; Hassenrück, Christiane; Salman-Carvalho, Verena; Holtappels, Moritz; Bienhold, Christina (2017): Amplicon sequencing of 16S rRNA of bacterial communities from deep-sea surface sediments in pressure and food source experiments. European Nucleotide Archive (ENA), insdc:PRJEB17614

Soltwedel, Thomas (2015): The Expedition PS93.2 of the Research Vessel POLARSTERN to the Fram Strait in 2015. Berichte zur Polar- und Meeresforschung = Reports on Polar and Marine Research, 693, 68 pp, https://doi.org/10.2312/BzPM_0693_2015

Project(s):

Assessment of bacterial life and matter cycling in deep-sea surface sediments (ABYSS)

Funding:

Seventh Framework Programme (FP7), grant/award no. 294757 : Assessment of bacterial life and matter cycling in deep-sea surface sediments

Coverage:

Latitude: 79.065100 * Longitude: 4.181000

Date/Time Start: 2015-07-26T00:00:00 * Date/Time End: 2015-07-26T00:00:00

Minimum DEPTH, sediment/rock: 0.01 m * Maximum DEPTH, sediment/rock: 0.01 m

Event(s):

PS93/050-5/6 (HG_IV) * Latitude: 79.065100 * Longitude: 4.181000 * Date/Time: 2015-07-26T00:00:00 * Elevation: -2465.5 m * Location: North Greenland Sea * Campaign: (ARK-XXIX/2.2) * Basis: * Method/Device: Multicorer with television (TVMUC) * Comment: Averaged Event from PS93/050-5 and PS93/050-6

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Comment
1	Station label	Station		Hoffmann, Katy
2	Depth, top/min	Depth top	m	Hoffmann, Katy
3	Depth, bottom/max	Depth bot	m	Hoffmann, Katy
4	DEPTH, sediment/rock	Depth sed	m	Hoffmann, Katy	Geocode
5	Sample type	Samp type		Hoffmann, Katy
6	Treatment	Treat		Hoffmann, Katy	added to sediment slurry
7	Incubation duration	Inc dur	days	Hoffmann, Katy
8	Pressure	P	bar	Hoffmann, Katy
9	Replicates	Repl	#	Hoffmann, Katy	biological
10	Prokaryotes, abundance as single cells	Prok cell abund	#/cm³	Hoffmann, Katy
11	Cell counts, standard deviation	Cell count std dev	±	Hoffmann, Katy
12	beta-glucosidase activity	MUF-bglu	µmol/l/h	Hoffmann, Katy
13	Chitobiase activity	Chitobiase activity	µmol/l/h	Hoffmann, Katy
14	Oxygen	O2	µmol/l	Hoffmann, Katy
15	Respiration rate, oxygen, sediment	Resp O2 sed	µmol/cm³/day	Hoffmann, Katy	original unit in 23 days
16	Accession number, genetics	Access no gen		Hoffmann, Katy	Metagenomic, sequence file
17	Accession number, genetics	Access no gen		Hoffmann, Katy	Metatranscriptomic, squence file

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Size:

1540 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Station	2 Depth top [m]	3 Depth bot [m]	4 Depth sed [m]	5 Samp type	6 Treat (added to sediment slurry)	7 Inc dur [days]	8 P [bar]	9 Repl [#] (biological)	10 Prok cell abund [#/cm³]	11 Cell count std dev [±]	12 MUF-bglu [µmol/l/h]	13 Chitobiase activity [µmol/l/h]	14 O2 [µmol/l]	15 Resp O2 sed [µmol/cm³/day] (original unit in 23 days)	16 Access no gen (Metagenomic, sequence file)	17 Access no gen (Metatranscriptomic, squence file)
HGIV	0	0.02	0.01	sediment	unfed	0	1.01325	1	1.30E9	1.70E8	0.14	0.37	300	0.00	insdc:ERR1716665	insdc:ERR1716709
HGIV	0	0.02	0.01	sediment	unfed	0	1.01325	2	1.50E9	2.10E8	0.16	0.44	300	0.00	insdc:ERR1716666
HGIV	0	0.02	0.01	sediment	unfed	0	1.01325	3	1.40E9	1.10E8	0.15	0.46	300	0.00	insdc:ERR1716667
HGIV	0	0.02	0.01	sediment	unfed	0	1.01325	4			0.15	0.45	300	0.00
HGIV	0	0.02	0.01	sediment	unfed	0	1.01325	5			0.14	0.49	300	0.00
HGIV	0	0.02	0.01	sediment	chitin	0	1.01325	1	1.20E9	2.20E8	0.12	0.38	300	0.00
HGIV	0	0.02	0.01	sediment	chitin	0	1.01325	2	1.30E9	1.90E8	0.12	0.44	300	0.00
HGIV	0	0.02	0.01	sediment	chitin	0	1.01325	3	1.30E9	2.40E8	0.11	0.39	300	0.00
HGIV	0	0.02	0.01	sediment	chitin	0	1.01325	4			0.14	0.42	300	0.00
HGIV	0	0.02	0.01	sediment	chitin	0	1.01325	5			0.16	0.43	300	0.00
HGIV				water	Control: chitin+seawater	0	1.01325	1			0.00	0.00	300	0.00
HGIV				water	Control: chitin+seawater	0	1.01325	2			0.00	0.00	300	0.00
HGIV	0	0.02	0.01	sediment	Thalassiosira weissflogii	0	1.01325	1	1.20E9	2.10E8	0.11	0.56	300	0.00
HGIV	0	0.02	0.01	sediment	Thalassiosira weissflogii	0	1.01325	2	1.30E9	1.80E8	0.12	0.54	300	0.00
HGIV	0	0.02	0.01	sediment	Thalassiosira weissflogii	0	1.01325	3	1.30E9	2.50E8	0.13	0.61	300	0.00
HGIV	0	0.02	0.01	sediment	Thalassiosira weissflogii	0	1.01325	4			0.10	0.67	300	0.00
HGIV	0	0.02	0.01	sediment	Thalassiosira weissflogii	0	1.01325	5			0.14	0.53	300	0.00
HGIV				water	Control: Thalassiosira weissflogii+seawater	0	1.01325	1			0.00	0.00	300	0.00
HGIV				water	Control: Thalassiosira weissflogii+seawater	0	1.01325	2			0.00	0.00	300	0.00
HGIV	0	0.02	0.01	sediment	Emiliania huxleyi	0	1.01325	1	1.40E9	2.00E8	0.12	0.51	300	0.00
HGIV	0	0.02	0.01	sediment	Emiliania huxleyi	0	1.01325	2	1.20E9	1.70E8	0.11	0.52	300	0.00
HGIV	0	0.02	0.01	sediment	Emiliania huxleyi	0	1.01325	3	1.40E9	2.50E8	0.13	0.48	300	0.00
HGIV	0	0.02	0.01	sediment	Emiliania huxleyi	0	1.01325	4			0.15	0.49	300	0.00
HGIV	0	0.02	0.01	sediment	Emiliania huxleyi	0	1.01325	5			0.11	0.51	300	0.00
HGIV				water	Control: Emiliania huxleyi+seawater	0	1.01325	1			0.00	0.00	300	0.00
HGIV				water	Control: Emiliania huxleyi+seawater	0	1.01325	2			0.00	0.00	300	0.00
HGIV	0	0.02	0.01	sediment	Bacillaria sp.	0	1.01325	1	1.30E9	2.40E8	0.12	0.86	300	0.00
HGIV	0	0.02	0.01	sediment	Bacillaria sp.	0	1.01325	2	1.40E9	1.70E8	0.12	0.74	300	0.00
HGIV	0	0.02	0.01	sediment	Bacillaria sp.	0	1.01325	3	1.30E9	2.40E8	0.12	0.70	300	0.00
HGIV	0	0.02	0.01	sediment	Bacillaria sp.	0	1.01325	4			0.11	0.77	300	0.00
HGIV	0	0.02	0.01	sediment	Bacillaria sp.	0	1.01325	5			0.11	0.79	300	0.00
HGIV				water	Control: Bacillaria sp.+seawater	0	1.01325	1			0.00	0.00	300	0.00
HGIV				water	Control: Bacillaria sp.+seawater	0	1.01325	2			0.00	0.00	300	0.00
HGIV	0	0.02	0.01	sediment	Melosira arctica	0	1.01325	1	1.40E9	2.10E8	0.13	0.66	300	0.00
HGIV	0	0.02	0.01	sediment	Melosira arctica	0	1.01325	2	1.30E9	2.10E8	0.12	0.60	300	0.00
HGIV	0	0.02	0.01	sediment	Melosira arctica	0	1.01325	3	1.40E9	2.30E8	0.11	0.55	300	0.00
HGIV	0	0.02	0.01	sediment	Melosira arctica	0	1.01325	4			0.12	0.60	300	0.00
HGIV	0	0.02	0.01	sediment	Melosira arctica	0	1.01325	5			0.12	0.61	300	0.00
HGIV				water	Control: Melosira arctica+seawater	0	1.01325	1			0.00	0.00	300	0.00
HGIV				water	Control: Melosira arctica+seawater	0	1.01325	2			0.00	0.00	300	0.00
HGIV	0	0.02	0.01	sediment	unfed	23	1.01325	1	2.10E9	2.00E8	0.18	0.70	181	0.13	insdc:ERR1716668	insdc:ERR1716705
HGIV	0	0.02	0.01	sediment	unfed	23	1.01325	2	2.40E9	2.60E8	0.22	0.66	180	0.13	insdc:ERR1716669
HGIV	0	0.02	0.01	sediment	unfed	23	1.01325	3	2.40E9	2.40E8	0.22	0.69	182	0.13	insdc:ERR1716670
HGIV	0	0.02	0.01	sediment	unfed	23	1.01325	4			0.22	0.63	190	0.12
HGIV	0	0.02	0.01	sediment	unfed	23	1.01325	5			0.21	0.64	179	0.13
HGIV	0	0.02	0.01	sediment	chitin	23	1.01325	1	4.10E9	2.50E8	1.82	6.82	40	0.27	insdc:ERR1716671	insdc:ERR1716706
HGIV	0	0.02	0.01	sediment	chitin	23	1.01325	2	3.80E9	2.50E8	1.89	6.61	40	0.27
HGIV	0	0.02	0.01	sediment	chitin	23	1.01325	3	4.10E9	2.00E8	1.81	6.83	42	0.26	insdc:ERR1716672
HGIV	0	0.02	0.01	sediment	chitin	23	1.01325	4			1.82	6.71	49	0.26
HGIV	0	0.02	0.01	sediment	chitin	23	1.01325	5			1.68	6.71	41	0.27
HGIV				water	Control: chitin+seawater	23	1.01325	1			0.00	0.00	300	0.00
HGIV				water	Control: chitin+seawater	23	1.01325	2			0.00	0.00	300	0.00
HGIV	0	0.02	0.01	sediment	Thalassiosira weissflogii	23	1.01325	1	2.60E9	2.40E8	2.15	5.74	62	0.22	insdc:ERR1716673	insdc:ERR1716708
HGIV	0	0.02	0.01	sediment	Thalassiosira weissflogii	23	1.01325	2	2.70E9	2.10E8	2.28	5.44	60	0.22	insdc:ERR1716674
HGIV	0	0.02	0.01	sediment	Thalassiosira weissflogii	23	1.01325	3	2.80E9	2.20E8	2.35	5.40	60	0.22	insdc:ERR1716698
HGIV	0	0.02	0.01	sediment	Thalassiosira weissflogii	23	1.01325	4			2.21	5.35	70	0.21
HGIV	0	0.02	0.01	sediment	Thalassiosira weissflogii	23	1.01325	5			2.29	5.53	61	0.22
HGIV				water	Control: Thalassiosira weissflogii+seawater	23	1.01325	1			0.00	0.00	300	0.00	insdc:ERR1716680
HGIV				water	Control: Thalassiosira weissflogii+seawater	23	1.01325	2			0.00	0.00	300	0.00
HGIV	0	0.02	0.01	sediment	Emiliania huxleyi	23	1.01325	1	2.80E9	2.80E8	6.40	5.21	90	0.20	insdc:ERR1716663	insdc:ERR1716707
HGIV	0	0.02	0.01	sediment	Emiliania huxleyi	23	1.01325	2	2.50E9	1.90E8	6.23	4.94	80	0.21	insdc:ERR1716664
HGIV	0	0.02	0.01	sediment	Emiliania huxleyi	23	1.01325	3	2.90E9	1.80E8	6.23	4.90	85	0.21	insdc:ERR1716697
HGIV	0	0.02	0.01	sediment	Emiliania huxleyi	23	1.01325	4			6.08	5.04	91	0.20
HGIV	0	0.02	0.01	sediment	Emiliania huxleyi	23	1.01325	5			6.29	5.03	90	0.20
HGIV				water	Control: Emiliania huxleyi+seawater	23	1.01325	1			0.00	0.00	300	0.00	insdc:ERR1716679
HGIV				water	Control: Emiliania huxleyi+seawater	23	1.01325	2			0.00	0.00	300	0.00
HGIV	0	0.02	0.01	sediment	Bacillaria sp.	23	1.01325	1	2.50E9	1.70E8	3.50	10.39	60	0.20	insdc:ERR1716675	insdc:ERR1716712
HGIV	0	0.02	0.01	sediment	Bacillaria sp.	23	1.01325	2	2.60E9	1.90E8	3.47	10.54	61	0.20	insdc:ERR1716676
HGIV	0	0.02	0.01	sediment	Bacillaria sp.	23	1.01325	3	2.60E9	1.40E8	3.45	10.23	60	0.20	insdc:ERR1716699
HGIV	0	0.02	0.01	sediment	Bacillaria sp.	23	1.01325	4			3.53	10.58	62	0.20
HGIV	0	0.02	0.01	sediment	Bacillaria sp.	23	1.01325	5			3.57	10.41	59	0.20
HGIV				water	Control: Bacillaria sp.+seawater	23	1.01325	1			0.00	0.00	300	0.00	insdc:ERR1716681
HGIV				water	Control: Bacillaria sp.+seawater	23	1.01325	2			0.00	0.00	300	0.00
HGIV	0	0.02	0.01	sediment	Melosira arctica	23	1.01325	1	2.80E9		1.99	3.81	75	0.19	insdc:ERR1716677
HGIV	0	0.02	0.01	sediment	Melosira arctica	23	1.01325	2			1.81	3.57	70	0.19	insdc:ERR1716678
HGIV	0	0.02	0.01	sediment	Melosira arctica	23	1.01325	3			1.72	3.51	75	0.19	insdc:ERR1716700
HGIV	0	0.02	0.01	sediment	Melosira arctica	23	1.01325	4			1.79	3.53	73	0.19
HGIV	0	0.02	0.01	sediment	Melosira arctica	23	1.01325	5			1.72	3.53	74	0.19
HGIV				water	Control: Melosira arctica+seawater	23	1.01325	1			0.00	0.00	300	0.00
HGIV				water	Control: Melosira arctica+seawater	23	1.01325	2			0.00	0.00	300	0.00
HGIV	0	0.02	0.01	sediment	unfed	23	1.01325	1	2.30E9	1.90E8	0.13	0.50	10	0.31	insdc:ERR1716682	insdc:ERR1716713
HGIV	0	0.02	0.01	sediment	unfed	23	1.01325	2	2.20E9	1.70E8	0.11	0.45	10	0.31	insdc:ERR1716683
HGIV	0	0.02	0.01	sediment	unfed	23	1.01325	3	2.20E9	1.60E8	0.13	0.47	11	0.31	insdc:ERR1716684
HGIV	0	0.02	0.01	sediment	unfed	23	1.01325	4			0.15	0.53	8	0.32
HGIV	0	0.02	0.01	sediment	unfed	23	1.01325	5			0.11	0.52	8	0.32
HGIV	0	0.02	0.01	sediment	chitin	23	1.01325	1	2.30E9	2.10E8	0.91	6.55	7	0.30	insdc:ERR1716685	insdc:ERR1716714
HGIV	0	0.02	0.01	sediment	chitin	23	1.01325	2	2.30E9	2.00E8	0.96	6.53	8	0.30	insdc:ERR1716686
HGIV	0	0.02	0.01	sediment	chitin	23	1.01325	3	2.20E9	1.50E8	0.90	6.69	6	0.30	insdc:ERR1716687
HGIV	0	0.02	0.01	sediment	chitin	23	1.01325	4			0.99	6.74	5	0.30
HGIV	0	0.02	0.01	sediment	chitin	23	1.01325	5			0.75	6.62	5	0.30
HGIV				water	Control: chitin+seawater	23	1.01325	1			0.00	0.00	300	0.00
HGIV				water	Control: chitin+seawater	23	1.01325	2			0.00	0.00	300	0.00
HGIV	0	0.02	0.01	sediment	Thalassiosira weissflogii	23	1.01325	1	3.30E9	2.00E8	1.99	3.40	5	0.27	insdc:ERR1716690	insdc:ERR1716715
HGIV	0	0.02	0.01	sediment	Thalassiosira weissflogii	23	1.01325	2	3.40E9	2.10E8	1.91	3.54	3	0.27	insdc:ERR1716691
HGIV	0	0.02	0.01	sediment	Thalassiosira weissflogii	23	250.00000	3	3.40E9	1.90E8	2.11	3.60	2	0.27	insdc:ERR1716702
HGIV	0	0.02	0.01	sediment	Thalassiosira weissflogii	23	250.00000	4			1.95	3.48	2	0.27
HGIV	0	0.02	0.01	sediment	Thalassiosira weissflogii	23	250.00000	5			2.01	3.56	5	0.27
HGIV				water	Control: Thalassiosira weissflogii+seawater	23	250.00000	1			0.00	0.00	300	0.00
HGIV				water	Control: Thalassiosira weissflogii+seawater	23	250.00000	2			0.00	0.00	300	0.00
HGIV	0	0.02	0.01	sediment	Emiliania huxleyi	23	250.00000	1	3.20E9	2.20E8	1.67	3.14	5	0.28	insdc:ERR1716688	insdc:ERR1716710
HGIV	0	0.02	0.01	sediment	Emiliania huxleyi	23	250.00000	2	3.40E9	2.00E8	1.50	3.05	2	0.29	insdc:ERR1716689
HGIV	0	0.02	0.01	sediment	Emiliania huxleyi	23	250.00000	3	3.30E9	2.00E8	1.59	2.99	3	0.29	insdc:ERR1716701
HGIV	0	0.02	0.01	sediment	Emiliania huxleyi	23	250.00000	4			1.68	3.11	2	0.29
HGIV	0	0.02	0.01	sediment	Emiliania huxleyi	23	250.00000	5			1.44	3.12	4	0.29
HGIV		0.02	0.01	water	Control: Emiliania huxleyi+seawater	23	250.00000	1			0.00	0.00	300	0.00
HGIV	0	0.02	0.01	water	Control: Emiliania huxleyi+seawater	23	250.00000	2			0.00	0.00	300	0.00
HGIV	0	0.02	0.01	sediment	Bacillaria sp.	23	250.00000	1	2.60E9	1.90E8	1.36	2.50	2	0.25	insdc:ERR1716692	insdc:ERR1716711
HGIV	0	0.02	0.01	sediment	Bacillaria sp.	23	250.00000	2	2.40E9	1.70E8	1.39	2.72	2	0.25	insdc:ERR1716693
HGIV	0	0.02	0.01	sediment	Bacillaria sp.	23	250.00000	3	2.30E9	1.60E8	1.31	2.61	3	0.25	insdc:ERR1716703
HGIV	0	0.02	0.01	sediment	Bacillaria sp.	23	250.00000	4			1.23	2.67	5	0.25
HGIV	0	0.02	0.01	sediment	Bacillaria sp.	23	250.00000	5			1.22	2.59	2	0.25
HGIV				water	Control: Bacillaria sp.+seawater	23	250.00000	1			0.00	0.00	300	0.00
HGIV				water	Control: Bacillaria sp.+seawater	23	250.00000	2			0.00	0.00	300	0.00
HGIV	0	0.02	0.01	sediment	Melosira arctica	23	250.00000	1	2.40E9	2.10E8	0.99	1.62	9	0.25	insdc:ERR1716694	insdc:ERR1716716
HGIV	0	0.02	0.01	sediment	Melosira arctica	23	250.00000	2	2.40E9	1.80E8	0.97	1.51	7	0.25	insdc:ERR1716695
HGIV	0	0.02	0.01	sediment	Melosira arctica	23	250.00000	3	2.50E9	1.70E8	0.94	1.47	8	0.25	insdc:ERR1716704
HGIV	0	0.02	0.01	sediment	Melosira arctica	23	250.00000	4			0.77	1.45	8	0.25
HGIV	0	0.02	0.01	sediment	Melosira arctica	23	250.00000	5			0.80	1.50	7	0.25
HGIV				water	Control: Melosira arctica+seawater	23	250.00000	1			0.00	0.00	300	0.00	insdc:ERR1716696
HGIV				water	Control: Melosira arctica+seawater	23	250.00000	2			0.00	0.00	300	0.00