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Chowdhury, Asif Moinur; Sakka, Yvonne; Filser, Juliane: The effect of a reduction of nitrogen and phosphorus to eutrophic and mesotrophic conditions towards silver nanoparticle (AgNP) on freshwater green algae species [dataset publication series]. University of Bremen, General and Theoretical Ecology, PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.865101 (unpublished dataset), Supplement to: Chowdhury, Asif Moinur (in prep.): Nutrient deficiency increases sensitivity of algae towards silver nanoparticles [thesis]. Master Thesis in the program of MSc Ecology, University of Bremen, Germany

Abstract:
We investigated the sensitivity of algae towards silver nanoparticles with OECD test medium and lower nutrient concentrations under standard test conditions to improve comparability and to exclude any other confounding factor aside nutrient levels. Two unicellular freshwater microalgae Desmodesmus subspicatus and Raphidocelis subcapitata were chosen due to their status as standard test organisms for the algae growth inhibition test and the response to changes in nutrient supply was compared. The original medium was used as the reference (standard). For the other four media, the amount of either nitrogen or phosphorus in the medium was lowered from half (50%) to one-fourth (25 %) of that of the OECD guideline, resulting in the following media: 50% N, 25% N, 50% P and 25% P medium. As test substance, the OECD reference material NM-300K was used. For this reason, the characterization of AgNP was done using DLS and Absorption spectra (UV/vis). Actual silver concentrations and ionic silver concentrations were measured at the highest test concentration used (100 µg Ag L-1) in R. subcapitata treatments only to reduce the number of samples. All tests were run according to the OECD guideline 201 with sterilized 50 mL cell culture flask. Each medium was tested using the test conditions for culturing with 3 replicates. Test concentrations for both algae species were 0, 25, 50 and 100 µg Ag L-1 for OECD, 50% P and 25% P while for both N reductions, the silver concentrations were 0, 10, 25 and 100 µg Ag L-1. Samples for determining the algal density were taken at every 24 h.
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7 datasets

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Datasets listed in this publication series

  1. Chowdhury, AM; Sakka, Y; Filser, J (validated): Absorption spectra for AgNP in 5 different mediums at day 0 and day 3. https://doi.pangaea.de/10.1594/PANGAEA.864980
  2. Chowdhury, AM; Sakka, Y; Filser, J (validated): Dose response chart for Desmodesmus subspicatus growth in all 5 mediums. https://doi.pangaea.de/10.1594/PANGAEA.865010
  3. Chowdhury, AM; Sakka, Y; Filser, J (validated): Dose response chart for Raphidocelis subcapitata growth in all 5 mediums. https://doi.pangaea.de/10.1594/PANGAEA.865012
  4. Chowdhury, AM; Sakka, Y; Filser, J (validated): Particle characterization of AgNP at the beginning and after 72 hours in 5 different mediums. https://doi.pangaea.de/10.1594/PANGAEA.865013
  5. Chowdhury, AM; Sakka, Y; Filser, J (validated): Silver quantification with Raphidocelis subcapitata at day 3 in 5 different mediums at a nominal concentration of 100 µg Ag. https://doi.pangaea.de/10.1594/PANGAEA.865039
  6. Chowdhury, AM; Sakka, Y; Filser, J (validated): Total cell count per mL for Desmodesmus subspicatus in all 5 mediums from day 0 to day 3. https://doi.pangaea.de/10.1594/PANGAEA.865098
  7. Chowdhury, AM; Sakka, Y; Filser, J (validated): Total cell count per mL for Raphidocelis subcapitata in all 5 mediums from day 0 to day 3. https://doi.pangaea.de/10.1594/PANGAEA.865099