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Mustaffa, Nur Ili Hamizah (2016): Extracellular carbonic anhydrase concentration at the sea-surface microlayer and 1 m depth during METEOR cruise M117, Baltic Sea [dataset]. Institute for Chemistry and Biology of the Marine Environment, Carl von Ossietzky University Oldenburg, Germany, PANGAEA, https://doi.org/10.1594/PANGAEA.861762, Supplement to: Mustaffa, NIH (2017): Extracellular carbonic anhydrase: Method development and its application to natural seawater. Limnology and Oceanography-Methods, 15(5), 503-517, https://doi.org/10.1002/lom3.10182

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Abstract:
We developed an effective fluorometric technique to quantify extracellular carbonic anhydrase (eCA) present in natural seawater samples. The technique includes the separation of eCA from cells to achieve low detection limits through high signal : noise ratios. eCA was efficiently extracted from cell membranes by treatment with 0.1 M phosphate buffer containing 2.5 M NaCl. The free eCA specifically forms a fluorescent complex with dansylamide, and the detection limit of the complex is below 0.1 nM. We applied the technique to samples from different culture solutions and natural seawater collected from the Baltic Sea. We observed eCA concentrations to be in the range of 0.10-0.67 nM in natural seawater. The data indicated that this technique is very sensitive, accurate, and feasible for routine and shipboard measurement of eCA from natural seawater. It is therefore an effective and rapid tool to investigate the carbon acquisition of phytoplankton both in mono culture as well natural communities.
Coverage:
Median Latitude: 57.779052 * Median Longitude: 19.374729 * South-bound Latitude: 56.283500 * West-bound Longitude: 16.590500 * North-bound Latitude: 59.768170 * East-bound Longitude: 23.625330
Date/Time Start: 2015-08-01T12:20:00 * Date/Time End: 2015-08-12T11:31:02
Minimum DEPTH, water: m * Maximum DEPTH, water: m
Event(s):
M117_607-11 * Latitude Start: 56.287000 * Longitude Start: 16.590500 * Latitude End: 56.302670 * Longitude End: 16.618170 * Date/Time Start: 2015-08-01T11:14:00 * Date/Time End: 2015-08-01T15:03:00 * Elevation Start: -15.4 m * Elevation End: -23.6 m * Location: Baltic Sea * Campaign: M117 * Basis: Meteor (1986) * Method/Device: Buoy (BUOY)
M117_608-9 * Latitude Start: 56.283500 * Longitude Start: 16.647830 * Latitude End: 56.291330 * Longitude End: 16.662500 * Date/Time Start: 2015-08-02T07:29:00 * Date/Time End: 2015-08-02T15:14:00 * Elevation Start: -31.9 m * Elevation End: -39.7 m * Location: Baltic Sea * Campaign: M117 * Basis: Meteor (1986) * Method/Device: Buoy (BUOY)
M117_609-9 * Latitude Start: 56.286000 * Longitude Start: 16.718830 * Latitude End: 56.286000 * Longitude End: 16.718830 * Date/Time Start: 2015-08-03T08:04:00 * Date/Time End: 2015-08-03T08:21:59 * Elevation Start: -62.3 m * Elevation End: -62.2 m * Location: Baltic Sea * Campaign: M117 * Basis: Meteor (1986) * Method/Device: Buoy (BUOY)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEventMustaffa, Nur Ili Hamizah
2Latitude of eventLatitudeMustaffa, Nur Ili Hamizah
3Longitude of eventLongitudeMustaffa, Nur Ili Hamizah
4DEPTH, waterDepth watermMustaffa, Nur Ili HamizahGeocode – min (SML)
5DEPTH, waterDepth watermMustaffa, Nur Ili HamizahGeocode – max (ULW)
6Station labelStationMustaffa, Nur Ili Hamizah
7DATE/TIMEDate/TimeMustaffa, Nur Ili HamizahGeocode
8Extracellular carbonic anhydraseeCAnmol/lMustaffa, Nur Ili HamizahSML = microlayer
9Extracellular carbonic anhydraseeCAnmol/lMustaffa, Nur Ili HamizahULW = water at 1 m depth
10Enrichment factorEFMustaffa, Nur Ili HamizaheCA
11Chlorophyll aChl aµg/lMustaffa, Nur Ili Hamizah
12RatioRatioMustaffa, Nur Ili HamizahCA/Chl a in SML
13RatioRatioMustaffa, Nur Ili HamizahCA/Chl a in ULW
Size:
133 data points

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