Mustaffa, Nur Ili Hamizah (2016): Extracellular carbonic anhydrase concentration at the sea-surface microlayer and 1 m depth during METEOR cruise M117, Baltic Sea [dataset]. Institute for Chemistry and Biology of the Marine Environment, Carl von Ossietzky University Oldenburg, Germany, PANGAEA, https://doi.org/10.1594/PANGAEA.861762, Supplement to: Mustaffa, NIH (2017): Extracellular carbonic anhydrase: Method development and its application to natural seawater. Limnology and Oceanography-Methods, 15(5), 503-517, https://doi.org/10.1002/lom3.10182
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Abstract:
We developed an effective fluorometric technique to quantify extracellular carbonic anhydrase (eCA) present in natural seawater samples. The technique includes the separation of eCA from cells to achieve low detection limits through high signal : noise ratios. eCA was efficiently extracted from cell membranes by treatment with 0.1 M phosphate buffer containing 2.5 M NaCl. The free eCA specifically forms a fluorescent complex with dansylamide, and the detection limit of the complex is below 0.1 nM. We applied the technique to samples from different culture solutions and natural seawater collected from the Baltic Sea. We observed eCA concentrations to be in the range of 0.10-0.67 nM in natural seawater. The data indicated that this technique is very sensitive, accurate, and feasible for routine and shipboard measurement of eCA from natural seawater. It is therefore an effective and rapid tool to investigate the carbon acquisition of phytoplankton both in mono culture as well natural communities.
Coverage:
Median Latitude: 57.779052 * Median Longitude: 19.374729 * South-bound Latitude: 56.283500 * West-bound Longitude: 16.590500 * North-bound Latitude: 59.768170 * East-bound Longitude: 23.625330
Date/Time Start: 2015-08-01T12:20:00 * Date/Time End: 2015-08-12T11:31:02
Minimum DEPTH, water: 0 m * Maximum DEPTH, water: 1 m
Event(s):
M117_607-11 * Latitude Start: 56.287000 * Longitude Start: 16.590500 * Latitude End: 56.302670 * Longitude End: 16.618170 * Date/Time Start: 2015-08-01T11:14:00 * Date/Time End: 2015-08-01T15:03:00 * Elevation Start: -15.4 m * Elevation End: -23.6 m * Location: Baltic Sea * Campaign: M117 * Basis: Meteor (1986) * Method/Device: Buoy (BUOY)
M117_608-9 * Latitude Start: 56.283500 * Longitude Start: 16.647830 * Latitude End: 56.291330 * Longitude End: 16.662500 * Date/Time Start: 2015-08-02T07:29:00 * Date/Time End: 2015-08-02T15:14:00 * Elevation Start: -31.9 m * Elevation End: -39.7 m * Location: Baltic Sea * Campaign: M117 * Basis: Meteor (1986) * Method/Device: Buoy (BUOY)
M117_609-9 * Latitude Start: 56.286000 * Longitude Start: 16.718830 * Latitude End: 56.286000 * Longitude End: 16.718830 * Date/Time Start: 2015-08-03T08:04:00 * Date/Time End: 2015-08-03T08:21:59 * Elevation Start: -62.3 m * Elevation End: -62.2 m * Location: Baltic Sea * Campaign: M117 * Basis: Meteor (1986) * Method/Device: Buoy (BUOY)
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Mustaffa, Nur Ili Hamizah | |||
| 2 | Latitude of event | Latitude | Mustaffa, Nur Ili Hamizah | |||
| 3 | Longitude of event | Longitude | Mustaffa, Nur Ili Hamizah | |||
| 4 | DEPTH, water | Depth water | m | Mustaffa, Nur Ili Hamizah | Geocode – min (SML) | |
| 5 | DEPTH, water | Depth water | m | Mustaffa, Nur Ili Hamizah | Geocode – max (ULW) | |
| 6 | Station label | Station | Mustaffa, Nur Ili Hamizah | |||
| 7 | DATE/TIME | Date/Time | Mustaffa, Nur Ili Hamizah | Geocode | ||
| 8 | Extracellular carbonic anhydrase | eCA | nmol/l | Mustaffa, Nur Ili Hamizah | SML = microlayer | |
| 9 | Extracellular carbonic anhydrase | eCA | nmol/l | Mustaffa, Nur Ili Hamizah | ULW = water at 1 m depth | |
| 10 | Enrichment factor | EF | Mustaffa, Nur Ili Hamizah | eCA | ||
| 11 | Chlorophyll a | Chl a | µg/l | Mustaffa, Nur Ili Hamizah | ||
| 12 | Ratio | Ratio | Mustaffa, Nur Ili Hamizah | CA/Chl a in SML | ||
| 13 | Ratio | Ratio | Mustaffa, Nur Ili Hamizah | CA/Chl a in ULW |
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Size:
133 data points
Data
| 1 Event | 2 Latitude | 3 Longitude | 4 Depth water [m] (min (SML)) | 5 Depth water [m] (max (ULW)) | 6 Station | 7 Date/Time | 8 eCA [nmol/l] (SML = microlayer) | 9 eCA [nmol/l] (ULW = water at 1 m depth) | 10 EF (eCA) | 11 Chl a [µg/l] | 12 Ratio (CA/Chl a in SML) | 13 Ratio (CA/Chl a in ULW) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| M117_607-11 | 56.2870 | 16.5905 | 0 | 1 | up1 | 2015-08-01T12:20:00 | 0.09 | 0.09 | 1.0 | 0.60 | 0.15 | 0.15 |
| M117_607-11 | 56.2870 | 16.5905 | 0 | 1 | up1 | 2015-08-01T12:40:00 | 0.31 | 0.09 | 3.4 | 0.60 | 0.52 | 0.15 |
| M117_608-9 | 56.2835 | 16.6478 | 0 | 1 | up3 | 2015-08-02T08:52:01 | 0.51 | 0.12 | 4.3 | 0.60 | 0.85 | 0.20 |
| M117_608-9 | 56.2835 | 16.6478 | 0 | 1 | up3 | 2015-08-02T09:00:01 | 0.21 | 0.14 | 1.5 | 0.60 | 0.35 | 0.23 |
| M117_608-9 | 56.2835 | 16.6478 | 0 | 1 | Up3 | 2015-08-02T09:04:01 | 0.64 | 0.19 | 3.4 | 0.60 | 1.07 | 0.32 |
| M117_609-9 | 56.2860 | 16.7188 | 0 | 1 | up2 | 2015-08-03T10:49:13 | 0.46 | 0.42 | 1.1 | 0.10 | 4.60 | 4.20 |
| M117_609-9 | 56.2860 | 16.7188 | 0 | 1 | up2 | 2015-08-03T11:22:04 | 0.57 | 0.32 | 1.8 | 0.60 | 0.95 | 0.53 |
| M117_609-9 | 56.2860 | 16.7188 | 0 | 1 | up2 | 2015-08-03T12:19:00 | 0.43 | 0.32 | 1.3 | 0.60 | 0.72 | 0.53 |
| M117_614-24 | 58.5837 | 18.2312 | 0 | 1 | tf0284 | 2015-08-06T07:20:04 | 0.14 | 0.12 | 1.2 | 3.80 | 0.04 | 0.03 |
| M117_614-24 | 58.5837 | 18.2312 | 0 | 1 | tf0284 | 2015-08-06T07:55:04 | 0.16 | 0.12 | 1.3 | 0.20 | 0.80 | 0.60 |
| M117_618-10 | 59.7680 | 23.6198 | 0 | 1 | up5 | 2015-08-08T10:48:03 | 0.09 | 0.34 | 0.3 | 6.60 | 0.01 | 0.05 |
| M117_618-10 | 59.7680 | 23.6198 | 0 | 1 | up5 | 2015-08-08T11:26:00 | 0.33 | 0.67 | 0.5 | 1.30 | 0.25 | 0.52 |
| M117_619-8 | 59.7000 | 23.2182 | 0 | 1 | up6 | 2015-08-09T08:52:00 | 0.13 | 0.10 | 1.3 | 1.00 | 0.13 | 0.10 |
| M117_619-8 | 59.7000 | 23.2182 | 0 | 1 | up6 | 2015-08-09T09:23:00 | 0.17 | 0.12 | 1.4 | 4.70 | 0.04 | 0.03 |
| M117_623-23 | 57.9998 | 19.9002 | 0 | 1 | tf0286 | 2015-08-11T08:16:01 | 0.28 | 0.11 | 2.5 | 1.90 | 0.15 | 0.06 |
| M117_629-9 | 57.3202 | 20.0507 | 0 | 1 | tf0271 | 2015-08-12T08:50:04 | 0.16 | 0.14 | 1.1 | 0.60 | 0.27 | 0.23 |
| M117_629-9 | 57.3202 | 20.0507 | 0 | 1 | tf0271 | 2015-08-12T09:22:04 | 0.11 | 0.20 | 0.6 | 1.30 | 0.08 | 0.15 |
| M117_629-9 | 57.3202 | 20.0507 | 0 | 1 | tf0271 | 2015-08-12T11:31:02 | 0.17 | 0.13 | 1.3 | 1.30 | 0.13 | 0.10 |
| M117_629-9 | 57.3202 | 20.0507 | 0 | 1 | tf0271 | 2015-08-12T09:22:05 | 0.20 | 0.27 | 0.7 | 1.00 | 0.20 | 0.27 |