Yan, Shi; Fuchs, Bernhard M; Lenk, Sabine; Harder, Jens; Wulf, Jörg; Jiao, Nianzhi; Amann, Rudolf: NOR5/OM60 clade of the Yangtze River estuary [dataset]. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.860515 (unpublished dataset), Supplement to: Yan, S et al. (2009): Biogeography and phylogeny of the NOR5/OM60 clade of Gammaproteobacteria. Systematic and Applied Microbiology, 32(2), 124-139, https://doi.org/10.1016/j.syapm.2008.12.001
Abstract:
The phylogeny, abundance, and biogeography of the NOR5/OM60 clade was investigated. This clade includes "Congregibacter litoralis" strain KT71, the first cultured representative of marine aerobic anoxygenic phototrophic Gammaproteobacteria. Most of the NOR5/OM60 sequences were retrieved from marine coastal settings, whereas there were fewer from open-ocean surface waters, deep-sea sediment, freshwater, saline lakes and soil. The abundance of members of the NOR5/OM60 clade in various marine sites was determined by fluorescence in situ hybridization using a newly designed and optimized probe set. Relative abundances in coastal marine waters off the Yangtze estuary were up to 3% of the total 4',6-diamidino-2-phenylindole (DAPI) counts. A small cruise was undertaken from 2006-09-06 to 2006-09-08 in the Yangtze River estuary. Samples were taken from surface water, and immediately fixed with 1% paraformaldehyde (PFA) for 1 h, filtered onto polycarbonate filters (Millipore, 47 mm in diameter, 0.2 µm pore size) and stored frozen at -20 °C.
Coverage:
Median Latitude: 31.398182 * Median Longitude: 122.819091 * South-bound Latitude: 30.500000 * West-bound Longitude: 121.600000 * North-bound Latitude: 32.000000 * East-bound Longitude: 123.500000
Date/Time Start: 2006-09-06T00:00:00 * Date/Time End: 2006-09-08T00:00:00
Event(s):
CJ-1 * Latitude: 32.000000 * Longitude: 122.010000 * Date/Time Start: 2006-09-06T00:00:00 * Date/Time End: 2006-09-08T00:00:00 * Location: Yangtze River (Yangtze) * Method/Device: Surface water sample (SWS)
CJ-2 * Latitude: 32.000000 * Longitude: 122.500000 * Date/Time Start: 2006-09-06T00:00:00 * Date/Time End: 2006-09-08T00:00:00 * Location: Yangtze River (Yangtze) * Method/Device: Surface water sample (SWS)
CJ-3 * Latitude: 32.000000 * Longitude: 123.000000 * Date/Time Start: 2006-09-06T00:00:00 * Date/Time End: 2006-09-08T00:00:00 * Location: Yangtze River (Yangtze) * Method/Device: Surface water sample (SWS)
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Yan, Shi | |||
| 2 | Date/Time of event | Date/Time | Yan, Shi | |||
| 3 | Latitude of event | Latitude | Yan, Shi | |||
| 4 | Longitude of event | Longitude | Yan, Shi | |||
| 5 | Salinity | Sal | Yan, Shi | |||
| 6 | Temperature, water | Temp | °C | Yan, Shi | ||
| 7 | NOR5 clade, targeted with NOR5/OM60 oligonucleotides FISH-probe | NOR5/OM60 | % | Yan, Shi | Fluorescence in situ hybridization (FISH) | |
| 8 | Standard deviation | Std dev | ± | Yan, Shi | Fluorescence in situ hybridization (FISH) | of NOR5 clade, targed with NOR5/OM60 oligonucleotides FISH-probe |
| 9 | Prokaryotes, number of cell | Prok cell abund | 109 #/ml | Yan, Shi | Epifluorescence microscopy after DAPI staining | original unit = 10**6 cells/ml |
| 10 | Chlorophyll a | Chl a | µg/l | Yan, Shi |
Size:
59 data points