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Schlegel, Peter; Binet, Monique T; Havenhand, Jonathan N; Doyle, Christopher J; Williamson, Jane E (2015): Ocean acidification impacts on sperm mitochondrial membrane potential bring sperm swimming behaviour near its tipping point [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.848011, Supplement to: Schlegel, P et al. (2015): Ocean acidification impacts on sperm mitochondrial membrane potential bring sperm swimming behaviour near its tipping point. Journal of Experimental Biology, 218(7), 1084-1090, https://doi.org/10.1242/jeb.114900

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Abstract:
Broadcast spawning marine invertebrates are susceptible to environmental stressors such as climate change, as their reproduction depends on the successful meeting and fertilization of gametes in the water column. Under near-future scenarios of ocean acidification, the swimming behaviour of marine invertebrate sperm is altered. We tested whether this was due to changes in sperm mitochondrial activity by investigating the effects of ocean acidification on sperm metabolism and swimming behaviour in the sea urchin Centrostephanus rodgersii. We used a fluorescent molecular probe (JC-1) and flow cytometry to visualize mitochondrial activity (measured as change in mitochondrial membrane potential, MMP). Sperm MMP was significantly reduced in delta pH -0.3 (35% reduction) and delta pH -0.5 (48% reduction) treatments, whereas sperm swimming behaviour was less sensitive with only slight changes (up to 11% decrease) observed overall. There was significant inter-individual variability in responses of sperm swimming behaviour and MMP to acidified seawater. We suggest it is likely that sperm exposed to these changes in pH are close to their tipping point in terms of physiological tolerance to acidity. Importantly, substantial inter-individual variation in responses of sperm swimming to ocean acidification may increase the scope for selection of resilient phenotypes, which, if heritable, could provide a basis for adaptation to future ocean acidification.
Keyword(s):
Animalia; Behaviour; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Centrostephanus rodgersii; Coast and continental shelf; Echinodermata; Laboratory experiment; Reproduction; Single species; South Pacific; Temperate
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.6. https://cran.r-project.org/package=seacarb
Project(s):
Ocean Acidification International Coordination Centre (OA-ICC)
Coverage:
Latitude: -33.800280 * Longitude: 151.267500
Date/Time Start: 2012-08-01T00:00:00 * Date/Time End: 2012-08-31T00:00:00
Event(s):
Fairlight_OA * Latitude: -33.800280 * Longitude: 151.267500 * Date/Time Start: 2012-08-01T00:00:00 * Date/Time End: 2012-08-31T00:00:00 * Method/Device: Experiment (EXP)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-07-09.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1SpeciesSpeciesBinet, Monique T
2TreatmentTreatBinet, Monique T
3IdentificationIDBinet, Monique Tdate
4IdentificationIDBinet, Monique Tmale
5PercentagePerc%Binet, Monique Tmotile sperm
6Percentage, standard errorPerc std e±Binet, Monique Tmotile sperm
7Motile sperm, speedSperm speedµm/sBinet, Monique T
8Speed, standard errorSp std e±Binet, Monique Tmotile sperm
9Mitochondrial activityMitochondrial activityBinet, Monique TFL2/FL1 ratio
10Mitochondrial activity, standard errorMitochondrial activity std e±Binet, Monique TFL2/FL1 ratio
11pHpHBinet, Monique TPotentiometricNBS scale
12pH, standard errorpH std e±Binet, Monique TPotentiometricNBS scale
13Temperature, waterTemp°CBinet, Monique T
14Temperature, water, standard errorT std e±Binet, Monique T
15SalinitySalBinet, Monique T
16Salinity, standard errorSal std e±Binet, Monique T
17Alkalinity, totalATµmol/kgBinet, Monique TPotentiometric titration
18Alkalinity, total, standard errorAT std e±Binet, Monique TPotentiometric titration
19Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmBinet, Monique T
20Calcite saturation stateOmega CalBinet, Monique TCalculated using CO2SYS
21Aragonite saturation stateOmega ArgBinet, Monique TCalculated using CO2SYS
22Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
23pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
24Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
25Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
26Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
27Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
28Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
29Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
30Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
31Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
837 data points

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