Citation:

jeb.114900

Name: Ocean acidification impacts on sperm mitochondrial membrane potential bring sperm swimming behaviour near its tipping point
Published: 2015-07-10
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Behaviour; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Centrostephanus rodgersii; Coast and continental shelf; Echinodermata; Laboratory experiment; Reproduction; Single species; South Pacific; Temperate

Always quote citation above when using data! You can download the citation in several formats below.

RIS Citation BibTeX Citation Text Citation Facebook TwitterShow Map Google Earth

Abstract:

Broadcast spawning marine invertebrates are susceptible to environmental stressors such as climate change, as their reproduction depends on the successful meeting and fertilization of gametes in the water column. Under near-future scenarios of ocean acidification, the swimming behaviour of marine invertebrate sperm is altered. We tested whether this was due to changes in sperm mitochondrial activity by investigating the effects of ocean acidification on sperm metabolism and swimming behaviour in the sea urchin Centrostephanus rodgersii. We used a fluorescent molecular probe (JC-1) and flow cytometry to visualize mitochondrial activity (measured as change in mitochondrial membrane potential, MMP). Sperm MMP was significantly reduced in delta pH -0.3 (35% reduction) and delta pH -0.5 (48% reduction) treatments, whereas sperm swimming behaviour was less sensitive with only slight changes (up to 11% decrease) observed overall. There was significant inter-individual variability in responses of sperm swimming behaviour and MMP to acidified seawater. We suggest it is likely that sperm exposed to these changes in pH are close to their tipping point in terms of physiological tolerance to acidity. Importantly, substantial inter-individual variation in responses of sperm swimming to ocean acidification may increase the scope for selection of resilient phenotypes, which, if heritable, could provide a basis for adaptation to future ocean acidification.

Keyword(s):

Animalia; Behaviour; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Centrostephanus rodgersii; Coast and continental shelf; Echinodermata; Laboratory experiment; Reproduction; Single species; South Pacific; Temperate

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.6. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: -33.800280 * Longitude: 151.267500

Date/Time Start: 2012-08-01T00:00:00 * Date/Time End: 2012-08-31T00:00:00

Event(s):

Fairlight_OA * Latitude: -33.800280 * Longitude: 151.267500 * Date/Time Start: 2012-08-01T00:00:00 * Date/Time End: 2012-08-31T00:00:00 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-07-09.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Binet, Monique T
2	Treatment	Treat		Binet, Monique T
3	Identification	ID		Binet, Monique T		date
4	Identification	ID		Binet, Monique T		male
5	Percentage	Perc	%	Binet, Monique T		motile sperm
6	Percentage, standard error	Perc std e	±	Binet, Monique T		motile sperm
7	Motile sperm, speed	Sperm speed	µm/s	Binet, Monique T
8	Speed, standard error	Sp std e	±	Binet, Monique T		motile sperm
9	Mitochondrial activity	Mitochondrial activity		Binet, Monique T		FL2/FL1 ratio
10	Mitochondrial activity, standard error	Mitochondrial activity std e	±	Binet, Monique T		FL2/FL1 ratio
11	pH	pH		Binet, Monique T	Potentiometric	NBS scale
12	pH, standard error	pH std e	±	Binet, Monique T	Potentiometric	NBS scale
13	Temperature, water	Temp	°C	Binet, Monique T
14	Temperature, water, standard error	T std e	±	Binet, Monique T
15	Salinity	Sal		Binet, Monique T
16	Salinity, standard error	Sal std e	±	Binet, Monique T
17	Alkalinity, total	AT	µmol/kg	Binet, Monique T	Potentiometric titration
18	Alkalinity, total, standard error	AT std e	±	Binet, Monique T	Potentiometric titration
19	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Binet, Monique T
20	Calcite saturation state	Omega Cal		Binet, Monique T	Calculated using CO2SYS
21	Aragonite saturation state	Omega Arg		Binet, Monique T	Calculated using CO2SYS
22	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
23	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
24	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
25	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
26	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
27	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
28	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
29	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
31	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

837 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Species	2 Treat	3 ID (date)	4 ID (male)	5 Perc [%] (motile sperm)	6 Perc std e [±] (motile sperm)	7 Sperm speed [µm/s]	8 Sp std e [±] (motile sperm)	9 Mitochondrial activity (FL2/FL1 ratio)	10 Mitochondrial activity std e [±] (FL2/FL1 ratio)	11 pH (NBS scale, Potentiometric)	12 pH std e [±] (NBS scale, Potentiometric)	13 Temp [°C]	14 T std e [±]	15 Sal	16 Sal std e [±]	17 AT [µmol/kg] (Potentiometric titration)	18 AT std e [±] (Potentiometric titration)	19 pCO2water_SST_wet [µatm]	20 Omega Cal (Calculated using CO2SYS)	21 Omega Arg (Calculated using CO2SYS)	22 CSC flag (Calculated using seacarb afte...)	23 pH (total scale, Calculated using...)	24 CO2 [µmol/kg] (Calculated using seacarb afte...)	25 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	26 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	27 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	28 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	29 DIC [µmol/kg] (Calculated using seacarb afte...)	30 Omega Arg (Calculated using seacarb afte...)	31 Omega Cal (Calculated using seacarb afte...)
Centrostephanus rodgersii (echinoderm)	pH 8.1	1	1	65.54	1.64	101.48	5.75	0.25	0.01	8.1	0.01	18	1	35.5	0.1	2106	4	435	3.4	2.2	26	7.98	14.82	433.44	434.95	1750.20	141.09	1906.11	2.17	3.36
Centrostephanus rodgersii (echinoderm)	pH 8.1	1	2	71.10	0.96	89.20	1.82	0.18	0.01	8.1	0.01	18	1	35.5	0.1	2106	4	435	3.4	2.2	26	7.98	14.82	433.44	434.95	1750.20	141.09	1906.11	2.17	3.36
Centrostephanus rodgersii (echinoderm)	pH 8.1	1	3	52.61	1.13	58.67	3.28	0.21	0.01	8.1	0.01	18	1	35.5	0.1	2106	4	435	3.4	2.2	26	7.98	14.82	433.44	434.95	1750.20	141.09	1906.11	2.17	3.36
Centrostephanus rodgersii (echinoderm)	pH 8.1	1	4	44.28	1.68	54.70	4.17	0.26	0.01	8.1	0.01	18	1	35.5	0.1	2106	4	435	3.4	2.2	26	7.98	14.82	433.44	434.95	1750.20	141.09	1906.11	2.17	3.36
Centrostephanus rodgersii (echinoderm)	pH 8.1	1	5	61.94	1.91	83.81	3.05	0.24	0.01	8.1	0.01	18	1	35.5	0.1	2106	4	435	3.4	2.2	26	7.98	14.82	433.44	434.95	1750.20	141.09	1906.11	2.17	3.36
Centrostephanus rodgersii (echinoderm)	pH 8.1	2	6	43.09	0.94	42.58	1.07	0.22	0.01	8.1	0.01	18	1	35.5	0.1	2106	4	435	3.4	2.2	26	7.98	14.82	433.44	434.95	1750.20	141.09	1906.11	2.17	3.36
Centrostephanus rodgersii (echinoderm)	pH 8.1	2	7	41.29	2.09	64.34	3.90	0.16	0.01	8.1	0.01	18	1	35.5	0.1	2106	4	435	3.4	2.2	26	7.98	14.82	433.44	434.95	1750.20	141.09	1906.11	2.17	3.36
Centrostephanus rodgersii (echinoderm)	pH 8.1	2	8	28.57	2.18	47.50	2.86	0.19	0.01	8.1	0.01	18	1	35.5	0.1	2106	4	435	3.4	2.2	26	7.98	14.82	433.44	434.95	1750.20	141.09	1906.11	2.17	3.36
Centrostephanus rodgersii (echinoderm)	pH 8.1	2	9	44.07	1.76	58.87	1.66	0.20	0.01	8.1	0.01	18	1	35.5	0.1	2106	4	435	3.4	2.2	26	7.98	14.82	433.44	434.95	1750.20	141.09	1906.11	2.17	3.36
Centrostephanus rodgersii (echinoderm)	pH 7.8	1	1	66.80	2.00	102.32	3.55	0.22	<0.01	7.8	0.01	18	1	35.5	0.1	2106	4	950	1.8	1.2	26	7.68	32.31	944.79	948.09	1911.50	77.21	2021.01	1.19	1.84
Centrostephanus rodgersii (echinoderm)	pH 7.8	1	2	65.87	3.10	90.53	2.84	0.14	<0.01	7.8	0.01	18	1	35.5	0.1	2106	4	950	1.8	1.2	26	7.68	32.31	944.79	948.09	1911.50	77.21	2021.01	1.19	1.84
Centrostephanus rodgersii (echinoderm)	pH 7.8	1	3	69.87	2.42	86.54	4.22	0.13	0.01	7.8	0.01	18	1	35.5	0.1	2106	4	950	1.8	1.2	26	7.68	32.31	944.79	948.09	1911.50	77.21	2021.01	1.19	1.84
Centrostephanus rodgersii (echinoderm)	pH 7.8	1	4	50.55	2.28	67.69	3.40	0.17	<0.01	7.8	0.01	18	1	35.5	0.1	2106	4	950	1.8	1.2	26	7.68	32.31	944.79	948.09	1911.50	77.21	2021.01	1.19	1.84
Centrostephanus rodgersii (echinoderm)	pH 7.8	1	5	61.09	1.60	81.38	3.96	0.18	0.01	7.8	0.01	18	1	35.5	0.1	2106	4	950	1.8	1.2	26	7.68	32.31	944.79	948.09	1911.50	77.21	2021.01	1.19	1.84
Centrostephanus rodgersii (echinoderm)	pH 7.8	2	6	46.69	1.95	49.80	2.00	0.11	0.01	7.8	0.01	18	1	35.5	0.1	2106	4	950	1.8	1.2	26	7.68	32.31	944.79	948.09	1911.50	77.21	2021.01	1.19	1.84
Centrostephanus rodgersii (echinoderm)	pH 7.8	2	7	37.94	2.79	54.74	2.53	0.12	<0.01	7.8	0.01	18	1	35.5	0.1	2106	4	950	1.8	1.2	26	7.68	32.31	944.79	948.09	1911.50	77.21	2021.01	1.19	1.84
Centrostephanus rodgersii (echinoderm)	pH 7.8	2	8	31.22	2.59	41.82	2.64	0.10	0.01	7.8	0.01	18	1	35.5	0.1	2106	4	950	1.8	1.2	26	7.68	32.31	944.79	948.09	1911.50	77.21	2021.01	1.19	1.84
Centrostephanus rodgersii (echinoderm)	pH 7.8	2	9	45.92	2.02	61.71	2.57	0.11	0.01	7.8	0.01	18	1	35.5	0.1	2106	4	950	1.8	1.2	26	7.68	32.31	944.79	948.09	1911.50	77.21	2021.01	1.19	1.84
Centrostephanus rodgersii (echinoderm)	pH 7.6	1	1	65.96	2.50	92.43	3.35	0.18	0.01	7.6	0.01	18	1	35.5	0.1	2106	4	1558	1.2	0.8	26	7.48	53.03	1550.81	1556.23	1979.05	50.42	2082.50	0.78	1.20
Centrostephanus rodgersii (echinoderm)	pH 7.6	1	2	61.11	1.96	83.84	1.60	0.08	0.01	7.6	0.01	18	1	35.5	0.1	2106	4	1558	1.2	0.8	26	7.48	53.03	1550.81	1556.23	1979.05	50.42	2082.50	0.78	1.20
Centrostephanus rodgersii (echinoderm)	pH 7.6	1	3	58.18	2.34	62.75	2.63	0.09	0.01	7.6	0.01	18	1	35.5	0.1	2106	4	1558	1.2	0.8	26	7.48	53.03	1550.81	1556.23	1979.05	50.42	2082.50	0.78	1.20
Centrostephanus rodgersii (echinoderm)	pH 7.6	1	4	50.92	3.72	61.41	6.84	0.15	0.01	7.6	0.01	18	1	35.5	0.1	2106	4	1558	1.2	0.8	26	7.48	53.03	1550.81	1556.23	1979.05	50.42	2082.50	0.78	1.20
Centrostephanus rodgersii (echinoderm)	pH 7.6	1	5	59.48	3.55	78.24	4.39	0.15	0.01	7.6	0.01	18	1	35.5	0.1	2106	4	1558	1.2	0.8	26	7.48	53.03	1550.81	1556.23	1979.05	50.42	2082.50	0.78	1.20
Centrostephanus rodgersii (echinoderm)	pH 7.6	2	6	38.96	1.38	37.95	0.62	0.11	0.01	7.6	0.01	18	1	35.5	0.1	2106	4	1558	1.2	0.8	26	7.48	53.03	1550.81	1556.23	1979.05	50.42	2082.50	0.78	1.20
Centrostephanus rodgersii (echinoderm)	pH 7.6	2	7	27.33	2.03	45.91	2.86	0.11	<0.01	7.6	0.01	18	1	35.5	0.1	2106	4	1558	1.2	0.8	26	7.48	53.03	1550.81	1556.23	1979.05	50.42	2082.50	0.78	1.20
Centrostephanus rodgersii (echinoderm)	pH 7.6	2	8	25.76	1.92	36.58	0.98	0.07	0.01	7.6	0.01	18	1	35.5	0.1	2106	4	1558	1.2	0.8	26	7.48	53.03	1550.81	1556.23	1979.05	50.42	2082.50	0.78	1.20
Centrostephanus rodgersii (echinoderm)	pH 7.6	2	9	36.80	1.56	44.49	2.33	0.08	<0.01	7.6	0.01	18	1	35.5	0.1	2106	4	1558	1.2	0.8	26	7.48	53.03	1550.81	1556.23	1979.05	50.42	2082.50	0.78	1.20