Citation:

nph.13037

Name: The nitrogen costs of photosynthesis in a diatom under current and future pCO2
Published: 2015-07-07
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; Not applicable; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Thalassiosira pseudonana

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Abstract:

With each cellular generation, oxygenic photoautotrophs must accumulate abundant protein complexes that mediate light capture, photosynthetic electron transport and carbon fixation. In addition to this net synthesis, oxygenic photoautotrophs must counter the light-dependent photoinactivation of Photosystem II (PSII), using metabolically expensive proteolysis, disassembly, resynthesis and re-assembly of protein subunits. We used growth rates, elemental analyses and protein quantitations to estimate the nitrogen (N) metabolism costs to both accumulate the photosynthetic system and to maintain PSII function in the diatom Thalassiosira pseudonana, growing at two pCO2 levels across a range of light levels. The photosynthetic system contains c. 15-25% of total cellular N. Under low growth light, N (re)cycling through PSII repair is only c. 1% of the cellular N assimilation rate. As growth light increases to inhibitory levels, N metabolite cycling through PSII repair increases to c. 14% of the cellular N assimilation rate. Cells growing under the assumed future 750 ppmv pCO2 show higher growth rates under optimal light, coinciding with a lowered N metabolic cost to maintain photosynthesis, but then suffer greater photoinhibition of growth under excess light, coincident with rising costs to maintain photosynthesis. We predict this quantitative trait response to light will vary across taxa.

Keyword(s):

Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; Not applicable; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Thalassiosira pseudonana

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.6. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-07-03.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Campbell, Douglas A
2	Irradiance	E	µmol/m²/s	Campbell, Douglas A
3	Treatment	Treat		Campbell, Douglas A
4	Growth rate	µ	1/day	Campbell, Douglas A
5	Identification	ID		Campbell, Douglas A		turbidostat
6	Cell biovolume	Cell biovol	µm³	Campbell, Douglas A
7	Protein per cell	Protein/cell	pg/#	Campbell, Douglas A
8	Nitrogen content per cell	N/cell		Campbell, Douglas A
9	Chlorophyll a per cell	Chl a/cell	pg/#	Campbell, Douglas A
10	Ratio	Ratio		Campbell, Douglas A		nitrogen content of cellular Chl a/total nitrogen content
11	Chlorophyll c per cell	Chl c/cell	pg/#	Campbell, Douglas A
12	Ratio	Ratio		Campbell, Douglas A		nitrogen content of cellular Chl c/total nitrogen content
13	Ratio	Ratio		Campbell, Douglas A		nitrogen content of cellular Chl a and Chl c/total nitrogen content
14	Fucoxanthin chlorophyll protein per cell	FCP/cell	fmol/#	Campbell, Douglas A
15	Ratio	Ratio		Campbell, Douglas A		nitrogen content of cellular FCP/total nitrogen content
16	Photosynthetic protein, PsbC	PsaC	pmol/µg	Campbell, Douglas A
17	Ratio	Ratio		Campbell, Douglas A		nitrogen content of PSI estimated from PsaC protein content/total nitrogen content
18	Photosynthetic protein PsbA	PsbA	pmol/µg	Campbell, Douglas A
19	Ratio	Ratio		Campbell, Douglas A		nitrogen content of total Photosystem II (PSII) estimated from PsbA protein content/total nitrogen content
20	Photosynthetic protein PsbD	PsbD	pmol/µg	Campbell, Douglas A
21	Ratio	Ratio		Campbell, Douglas A		nitrogen content of total Photosystem II (PSII) estimated from PsbD protein content/total nitrogen content
22	Photosynthetic protein Rubisco	RbcL	pmol/mg	Campbell, Douglas A
23	Ratio	Ratio		Campbell, Douglas A		nitrogen content of RUBISCO estimated from RbcL protein content/total nitrogen content
24	Cytochrome c1	PetC	pmol/µg	Campbell, Douglas A
25	Ratio	Ratio		Campbell, Douglas A		nitrogen content of Cytochrome b6f complex, estimated from PetC protein content/total nitrogen content
26	Ratio	Ratio		Campbell, Douglas A		summed nitrogen content of the photosynthetic system/total nitrogen content
27	Temperature, water	Temp	°C	Campbell, Douglas A
28	Salinity	Sal		Campbell, Douglas A
29	Carbon, inorganic, dissolved	DIC	µmol/kg	Campbell, Douglas A
30	pH	pH		Campbell, Douglas A	Potentiometric	NBS scale
31	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	ppmv	Campbell, Douglas A	Calculated using CO2SYS
32	Alkalinity, total	AT	µmol/kg	Campbell, Douglas A	Calculated using CO2SYS
33	Bicarbonate ion	[HCO3]-	µmol/kg	Campbell, Douglas A	Calculated using CO2SYS
34	Carbonate ion	[CO3]2-	µmol/kg	Campbell, Douglas A	Calculated using CO2SYS
35	Carbon dioxide	CO2	µmol/kg	Campbell, Douglas A	Calculated using CO2SYS
36	Phosphate	[PO4]3-	µmol/l	Campbell, Douglas A
37	Silicate	Si(OH)4	µmol/l	Campbell, Douglas A
38	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
40	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1536 data points

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