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Li, Gang; Brown, Christopher M; Jeans, Jennifer A; Donaher, Natalie A; McCarthy, Avery; Campbell, Douglas A (2015): The nitrogen costs of photosynthesis in a diatom under current and future pCO2. PANGAEA, https://doi.org/10.1594/PANGAEA.847792, Supplement to: Li, G et al. (2015): The nitrogen costs of photosynthesis in a diatom under current and future pCO2. New Phytologist, 205(2), 533-543, https://doi.org/10.1111/nph.13037

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Abstract:
With each cellular generation, oxygenic photoautotrophs must accumulate abundant protein complexes that mediate light capture, photosynthetic electron transport and carbon fixation. In addition to this net synthesis, oxygenic photoautotrophs must counter the light-dependent photoinactivation of Photosystem II (PSII), using metabolically expensive proteolysis, disassembly, resynthesis and re-assembly of protein subunits. We used growth rates, elemental analyses and protein quantitations to estimate the nitrogen (N) metabolism costs to both accumulate the photosynthetic system and to maintain PSII function in the diatom Thalassiosira pseudonana, growing at two pCO2 levels across a range of light levels. The photosynthetic system contains c. 15-25% of total cellular N. Under low growth light, N (re)cycling through PSII repair is only c. 1% of the cellular N assimilation rate. As growth light increases to inhibitory levels, N metabolite cycling through PSII repair increases to c. 14% of the cellular N assimilation rate. Cells growing under the assumed future 750 ppmv pCO2 show higher growth rates under optimal light, coinciding with a lowered N metabolic cost to maintain photosynthesis, but then suffer greater photoinhibition of growth under excess light, coincident with rising costs to maintain photosynthesis. We predict this quantitative trait response to light will vary across taxa.
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloise (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.6. https://cran.r-project.org/package=seacarb
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-07-03.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1SpeciesSpeciesCampbell, Douglas A
2IrradianceEµmol/m2/sCampbell, Douglas A
3TreatmentTreatCampbell, Douglas A
4Growth rateµ1/dayCampbell, Douglas A
5IdentificationIDCampbell, Douglas Aturbidostat
6Cell biovolumeCell biovolµm3Campbell, Douglas A
7Protein per cellProteinpg/#Campbell, Douglas A
8Nitrogen content per cellN/cellCampbell, Douglas A
9Chlorophyll a per cellChl apg/#Campbell, Douglas A
10RatioRatioCampbell, Douglas Anitrogen content of cellular Chl a/total nitrogen content
11Chlorophyll c per cellChl cpg/#Campbell, Douglas A
12RatioRatioCampbell, Douglas Anitrogen content of cellular Chl c/total nitrogen content
13RatioRatioCampbell, Douglas Anitrogen content of cellular Chl a and Chl c/total nitrogen content
14Fucoxanthin chlorophyll protein per cellFCPfmol/#Campbell, Douglas A
15RatioRatioCampbell, Douglas Anitrogen content of cellular FCP/total nitrogen content
16Photosynthetic protein PsbCPsaCpmol/µgCampbell, Douglas A
17RatioRatioCampbell, Douglas Anitrogen content of PSI estimated from PsaC protein content/total nitrogen content
18Photosynthetic protein PsbAPsbApmol/µgCampbell, Douglas A
19RatioRatioCampbell, Douglas Anitrogen content of total Photosystem II (PSII) estimated from PsbA protein content/total nitrogen content
20Photosynthetic protein PsbDPsbDpmol/µgCampbell, Douglas A
21RatioRatioCampbell, Douglas Anitrogen content of total Photosystem II (PSII) estimated from PsbD protein content/total nitrogen content
22Photosynthetic protein RubiscoRbcLpmol/mgCampbell, Douglas A
23RatioRatioCampbell, Douglas Anitrogen content of RUBISCO estimated from RbcL protein content/total nitrogen content
24Cytochrome c1PetCpmol/µgCampbell, Douglas A
25RatioRatioCampbell, Douglas Anitrogen content of Cytochrome b6f complex, estimated from PetC protein content/total nitrogen content
26RatioRatioCampbell, Douglas Asummed nitrogen content of the photosynthetic system/total nitrogen content
27Temperature, waterTemp°CCampbell, Douglas A
28SalinitySalCampbell, Douglas A
29Carbon, inorganic, dissolvedDICµmol/kgCampbell, Douglas A
30pHpHCampbell, Douglas APotentiometricNBS scale
31Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetppmvCampbell, Douglas ACalculated using CO2SYS
32Alkalinity, totalATµmol/kgCampbell, Douglas ACalculated using CO2SYS
33Bicarbonate ion[HCO3]-µmol/kgCampbell, Douglas ACalculated using CO2SYS
34Carbonate ion[CO3]2-µmol/kgCampbell, Douglas ACalculated using CO2SYS
35Carbon dioxideCO2µmol/kgCampbell, Douglas ACalculated using CO2SYS
36Phosphate[PO4]3-µmol/lCampbell, Douglas A
37SilicateSi(OH)4µmol/lCampbell, Douglas A
38Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
39pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
40Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
41Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
42Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
43Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
44Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
45Alkalinity, totalATµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
46Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
47Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Size:
1536 data points

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