Citation:

nph.13037

Name: The nitrogen costs of photosynthesis in a diatom under current and future pCO2
Published: 2015-07-07
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; Not applicable; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Thalassiosira pseudonana

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Abstract:

With each cellular generation, oxygenic photoautotrophs must accumulate abundant protein complexes that mediate light capture, photosynthetic electron transport and carbon fixation. In addition to this net synthesis, oxygenic photoautotrophs must counter the light-dependent photoinactivation of Photosystem II (PSII), using metabolically expensive proteolysis, disassembly, resynthesis and re-assembly of protein subunits. We used growth rates, elemental analyses and protein quantitations to estimate the nitrogen (N) metabolism costs to both accumulate the photosynthetic system and to maintain PSII function in the diatom Thalassiosira pseudonana, growing at two pCO2 levels across a range of light levels. The photosynthetic system contains c. 15-25% of total cellular N. Under low growth light, N (re)cycling through PSII repair is only c. 1% of the cellular N assimilation rate. As growth light increases to inhibitory levels, N metabolite cycling through PSII repair increases to c. 14% of the cellular N assimilation rate. Cells growing under the assumed future 750 ppmv pCO2 show higher growth rates under optimal light, coinciding with a lowered N metabolic cost to maintain photosynthesis, but then suffer greater photoinhibition of growth under excess light, coincident with rising costs to maintain photosynthesis. We predict this quantitative trait response to light will vary across taxa.

Keyword(s):

Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; Not applicable; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Thalassiosira pseudonana

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.6. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-07-03.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Campbell, Douglas A
2	Irradiance	E	µmol/m²/s	Campbell, Douglas A
3	Treatment	Treat		Campbell, Douglas A
4	Growth rate	µ	1/day	Campbell, Douglas A
5	Identification	ID		Campbell, Douglas A		turbidostat
6	Cell biovolume	Cell biovol	µm³	Campbell, Douglas A
7	Protein per cell	Protein/cell	pg/#	Campbell, Douglas A
8	Nitrogen content per cell	N/cell		Campbell, Douglas A
9	Chlorophyll a per cell	Chl a/cell	pg/#	Campbell, Douglas A
10	Ratio	Ratio		Campbell, Douglas A		nitrogen content of cellular Chl a/total nitrogen content
11	Chlorophyll c per cell	Chl c/cell	pg/#	Campbell, Douglas A
12	Ratio	Ratio		Campbell, Douglas A		nitrogen content of cellular Chl c/total nitrogen content
13	Ratio	Ratio		Campbell, Douglas A		nitrogen content of cellular Chl a and Chl c/total nitrogen content
14	Fucoxanthin chlorophyll protein per cell	FCP/cell	fmol/#	Campbell, Douglas A
15	Ratio	Ratio		Campbell, Douglas A		nitrogen content of cellular FCP/total nitrogen content
16	Photosynthetic protein, PsbC	PsaC	pmol/µg	Campbell, Douglas A
17	Ratio	Ratio		Campbell, Douglas A		nitrogen content of PSI estimated from PsaC protein content/total nitrogen content
18	Photosynthetic protein PsbA	PsbA	pmol/µg	Campbell, Douglas A
19	Ratio	Ratio		Campbell, Douglas A		nitrogen content of total Photosystem II (PSII) estimated from PsbA protein content/total nitrogen content
20	Photosynthetic protein PsbD	PsbD	pmol/µg	Campbell, Douglas A
21	Ratio	Ratio		Campbell, Douglas A		nitrogen content of total Photosystem II (PSII) estimated from PsbD protein content/total nitrogen content
22	Photosynthetic protein Rubisco	RbcL	pmol/mg	Campbell, Douglas A
23	Ratio	Ratio		Campbell, Douglas A		nitrogen content of RUBISCO estimated from RbcL protein content/total nitrogen content
24	Cytochrome c1	PetC	pmol/µg	Campbell, Douglas A
25	Ratio	Ratio		Campbell, Douglas A		nitrogen content of Cytochrome b6f complex, estimated from PetC protein content/total nitrogen content
26	Ratio	Ratio		Campbell, Douglas A		summed nitrogen content of the photosynthetic system/total nitrogen content
27	Temperature, water	Temp	°C	Campbell, Douglas A
28	Salinity	Sal		Campbell, Douglas A
29	Carbon, inorganic, dissolved	DIC	µmol/kg	Campbell, Douglas A
30	pH	pH		Campbell, Douglas A	Potentiometric	NBS scale
31	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	ppmv	Campbell, Douglas A	Calculated using CO2SYS
32	Alkalinity, total	AT	µmol/kg	Campbell, Douglas A	Calculated using CO2SYS
33	Bicarbonate ion	[HCO3]-	µmol/kg	Campbell, Douglas A	Calculated using CO2SYS
34	Carbonate ion	[CO3]2-	µmol/kg	Campbell, Douglas A	Calculated using CO2SYS
35	Carbon dioxide	CO2	µmol/kg	Campbell, Douglas A	Calculated using CO2SYS
36	Phosphate	[PO4]3-	µmol/l	Campbell, Douglas A
37	Silicate	Si(OH)4	µmol/l	Campbell, Douglas A
38	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
40	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1536 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Species	2 E [µmol/m²/s]	3 Treat	4 µ [1/day]	5 ID (turbidostat)	6 Cell biovol [µm³]	7 Protein/cell [pg/#]	8 N/cell	9 Chl a/cell [pg/#]	10 Ratio (nitrogen content of cellular ...)	11 Chl c/cell [pg/#]	12 Ratio (nitrogen content of cellular ...)	13 Ratio (nitrogen content of cellular ...)	14 FCP/cell [fmol/#]	15 Ratio (nitrogen content of cellular ...)	16 PsaC [pmol/µg]	17 Ratio (nitrogen content of PSI estim...)	18 PsbA [pmol/µg]	19 Ratio (nitrogen content of total Pho...)	20 PsbD [pmol/µg]	21 Ratio (nitrogen content of total Pho...)	22 RbcL [pmol/mg]	23 Ratio (nitrogen content of RUBISCO e...)	24 PetC [pmol/µg]	25 Ratio (nitrogen content of Cytochrom...)	26 Ratio (summed nitrogen content of th...)	27 Temp [°C]	28 Sal	29 DIC [µmol/kg]	30 pH (NBS scale, Potentiometric)	31 pCO2water_SST_wet [ppmv] (Calculated using CO2SYS)	32 AT [µmol/kg] (Calculated using CO2SYS)	33 [HCO3]- [µmol/kg] (Calculated using CO2SYS)	34 [CO3]2- [µmol/kg] (Calculated using CO2SYS)	35 CO2 [µmol/kg] (Calculated using CO2SYS)	36 [PO4]3- [µmol/l]	37 Si(OH)4 [µmol/l]	38 CSC flag (Calculated using seacarb afte...)	39 pH (total scale, Calculated using...)	40 CO2 [µmol/kg] (Calculated using seacarb afte...)	41 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	42 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	43 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	44 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	45 AT [µmol/kg] (Calculated using seacarb afte...)	46 Omega Arg (Calculated using seacarb afte...)	47 Omega Cal (Calculated using seacarb afte...)
Thalassiosira pseudonana (phytoplankton)	35.7	pCO2=390 ppm	0.7439520	1	25.88	3.927	51870942628	0.21630	0.011241996	0.0267000	0.002029501	0.013271497	0.021851573	0.084477986	0.06962588	0.010495984	0.05668713	0.010652136	0.07377134	0.013862447	1.0115390	0.039067504			0.157965107	18	35	1976	8.14	312	2302	1750	215	10.7	21	52.5	27	8.02	14.01	410	409	1805	157	2221	2.43	3.76
Thalassiosira pseudonana (phytoplankton)	87.2	pCO2=390 ppm	1.5536400	2	25.00	5.122	57108661436	0.15730	0.007425707	0.0139000	0.000959655	0.008385361	0.011375913	0.039945627	0.05105659	0.009118116	0.07383202	0.016436064	0.04981519	0.011089574	1.4635520	0.066964098	0.07110616	0.004847491	0.145696758	18	35	1866	8.18	300	2212	1632	209	9.1	21	52.5	27	8.06	11.98	351	349	1692	162	2124	2.50	3.86
Thalassiosira pseudonana (phytoplankton)	183.7	pCO2=390 ppm	1.7482400	3	23.37	5.586	51238349594	0.11380	0.005987674	0.0032950	0.000253549	0.006241224	0.002696664	0.010553991	0.04043017	0.008776616	0.06947869	0.018800651	0.06341611	0.017160142	1.5055720	0.083734340	0.05959098	0.004938085	0.133044907	18	35	1902	8.10	332	2196	1700	191	11.4	21	52.5	27	7.98	14.88	436	434	1748	139	2122	2.15	3.32
Thalassiosira pseudonana (phytoplankton)	34.1	pCO2=390 ppm	0.9996000	1	17.21	4.703	70731521440	0.17070	0.006506262	0.0201700	0.001124333	0.007630596	0.016507349	0.046800374	0.08137733	0.010774103	0.06722272	0.011094137	0.06271843	0.010350769	0.7190493	0.024390288	0.03042753	0.001537804	0.102227302	18	35	1976	8.14	312	2302	1750	215	10.7	21	52.5	27	8.02	14.01	410	409	1805	157	2221	2.43	3.76
Thalassiosira pseudonana (phytoplankton)	84.6	pCO2=390 ppm	1.2831600	2	21.09	3.816	51707108096	0.11470	0.005980317	0.0267400	0.002038982	0.008019299	0.021884309	0.084872615	0.08777248	0.012898292	0.03125612	0.005725437	0.04652751	0.008522822	0.8376204	0.031535639	0.04505863	0.002527600	0.145578882	18	35	1866	8.18	300	2212	1632	209	9.1	21	52.5	27	8.06	11.98	351	349	1692	162	2124	2.50	3.86
Thalassiosira pseudonana (phytoplankton)	172.2	pCO2=390 ppm	1.5990800	3	25.00	4.201	56382179400	0.09281	0.004437762	0.0233100	0.001630056	0.006067818	0.019077160	0.067851092	0.06735468	0.009992964	0.06589833	0.012187109	0.07116563	0.013161234	1.5293040	0.058130080	0.06373743	0.003609754	0.157838817	18	35	1902	8.10	332	2196	1700	191	11.4	21	52.5	27	7.98	14.88	436	434	1748	139	2122	2.15	3.32
Thalassiosira pseudonana (phytoplankton)	30.5	pCO2=390 ppm	1.0399600	1	20.40	8.612	68912215020	0.35230	0.013782483	0.0665900	0.003809912	0.017592396	0.054497987	0.158587603	0.08338279	0.020749133	0.06790911	0.021064508	0.04973518	0.015427196	0.5829161	0.037162946	0.03302132	0.003136711	0.258293296	18	35	1976	8.14	312	2302	1750	215	10.7	21	52.5	27	8.02	14.01	410	409	1805	157	2221	2.43	3.76
Thalassiosira pseudonana (phytoplankton)	79.8	pCO2=390 ppm	1.2673600	2	22.58	6.869	82012714700	0.24600	0.008086581	0.0536500	0.002579234	0.010665815	0.043907749	0.107360609	0.04505606	0.007514174	0.05642126	0.011729259	0.03784458	0.007867405	0.6037819	0.025798158			0.163068015	18	35	1866	8.18	300	2212	1632	209	9.1	21	52.5	27	8.06	11.98	351	349	1692	162	2124	2.50	3.86
Thalassiosira pseudonana (phytoplankton)	164.9	pCO2=390 ppm	1.6640400	3	25.84	3.818	54893673484	0.10660	0.005235353	0.0237600	0.001706579	0.006941932	0.019445445	0.071036339	0.03344716	0.004632214	0.06695239	0.011558333	0.08051851	0.013900321	2.3386620	0.082980751	0.07709672	0.004075883	0.181225451	18	35	1902	8.10	332	2196	1700	191	11.4	21	52.5	27	7.98	14.88	436	434	1748	139	2122	2.15	3.32
Thalassiosira pseudonana (phytoplankton)	25.2	pCO2=390 ppm	0.7025067	1	20.40	9.241	68093765000	0.33220	0.013152349	0.0064160	0.000371500	0.013523849	0.005250925	0.015463701	0.10326870	0.027905901	0.05165009	0.017397954	0.10049230	0.033850095	1.0462320	0.072432904	0.04127238	0.004257389	0.150981698	18	35	1976	8.14	312	2302	1750	215	10.7	21	52.5	27	8.02	14.01	410	409	1805	157	2221	2.43	3.76
Thalassiosira pseudonana (phytoplankton)	72.4	pCO2=390 ppm	1.5816340	2	24.19	6.697	63016135040	0.18800	0.008042983	0.0157500	0.000985442	0.009028424	0.012889973	0.041019015	0.06872396	0.014542947	0.04729066	0.012474392	0.07626013	0.020115997	1.2207270	0.066182529	0.04856441	0.003923005	0.147170312	18	35	1866	8.18	300	2212	1632	209	9.1	21	52.5	27	8.06	11.98	351	349	1692	162	2124	2.50	3.86
Thalassiosira pseudonana (phytoplankton)	145.9	pCO2=390 ppm	1.8419100	3	27.61	5.423	58874558738	0.16720	0.007656313	0.0008694	0.000058223	0.007714535	0.000711527	0.002423530	0.03992439	0.007322611	0.07945792	0.018166222	0.10542340	0.024102630	1.9924770	0.093627091	0.07691687	0.005385249	0.134639237	18	35	1902	8.10	332	2196	1700	191	11.4	21	52.5	27	7.98	14.88	436	434	1748	139	2122	2.15	3.32
Thalassiosira pseudonana (phytoplankton)	230.4	pCO2=390 ppm	1.3323840	1	28.66	4.845	68052935840	0.10260	0.004064541	0.0029700	0.000172073	0.004236614	0.002430681	0.007162523	0.03812670	0.005404952	0.10061390	0.017779534	0.04885417	0.008633046	2.3607280	0.085741118	0.07677762	0.004154835	0.124479575	18	35	1908	8.10	321	2110	1630	184	11.0	21	52.5	27	7.98	14.93	437	435	1754	140	2128	2.15	3.33
Thalassiosira pseudonana (phytoplankton)	243.7	pCO2=390 ppm	1.5165300	2	26.78	6.266	64816050620	0.12490	0.005195064	0.0001938	0.000011789	0.005206853	0.000158608	0.000490713	0.05622150	0.010822459	0.16479760	0.039543416	0.08640186	0.020732248	1.7479380	0.086204537	0.08440373	0.006202142	0.148470120	18	35	1908	8.10	321	2110	1630	184	11.0	21	52.5	27	7.98	14.93	437	435	1754	140	2128	2.15	3.33
Thalassiosira pseudonana (phytoplankton)	243.3	pCO2=390 ppm	1.5613920	3	26.78	4.390	57562605818	0.07608	0.003563207	0.0130700	0.000895236	0.004458443	0.010696631	0.037264180	0.03852425	0.005850244	0.07221747	0.013670419	0.05819753	0.011016512	1.6792560	0.065333700	0.06861581	0.003977594	0.130554581	18	35	1908	8.10	321	2110	1630	184	11.0	21	52.5	27	7.98	14.93	437	435	1754	140	2128	2.15	3.33
Thalassiosira pseudonana (phytoplankton)	361.0	pCO2=390 ppm	1.3030630	1	26.78	5.146	59058711498	0.09219	0.004208341	0.0132600	0.000885241	0.005093582	0.010852130	0.036848175	0.03477594	0.006033654	0.08329102	0.018013557	0.03572497	0.007726328	1.7788280	0.079070784	0.07107208	0.004707139	0.149766891	18	35	1947	8.16	295	2286	1714	223	10.1	21	52.5	27	8.04	13.14	385	383	1772	162	2201	2.50	3.86
Thalassiosira pseudonana (phytoplankton)	385.8	pCO2=390 ppm	1.3347430	2	25.88	4.639	57311193340	0.07135	0.003356337	0.0020650	0.000142064	0.003498401	0.001690019	0.005913397	0.06623553	0.010675584	0.08079530	0.016232541	0.07824837	0.015720838	2.1658670	0.089436126	0.07478996	0.004601511	0.130357559	18	35	1947	8.16	295	2286	1714	223	10.1	21	52.5	27	8.04	13.14	385	383	1772	162	2201	2.50	3.86
Thalassiosira pseudonana (phytoplankton)	378.5	pCO2=390 ppm	1.3800000	3	27.70	6.416	66425430120	0.12080	0.004902794	0.0060290	0.000357860	0.005260653	0.004934200	0.014895920	0.03611973	0.006946886	0.11357090	0.027227798	0.07568896	0.018145878	1.6539610	0.081498857	0.07214372	0.005296643	0.141126759	18	35	1947	8.16	295	2286	1714	223	10.1	21	52.5	27	8.04	13.14	385	383	1772	162	2201	2.50	3.86
Thalassiosira pseudonana (phytoplankton)	32.6	pCO2=750 ppm	1.5576400	1	15.02	11.620		0.25000		0.0455000			0.037237699		0.03596396		0.05855833		0.04230962		1.0573810		0.05147653			18	35	2007	7.92	551	2213	1850	138	18.9	21	52.5	27	7.80	24.28	711	708	1884	99	2155	1.53	2.36
Thalassiosira pseudonana (phytoplankton)	82.8	pCO2=750 ppm	1.6695430	2	17.21	4.050	56110478804	0.12420	0.005967448	0.0170900	0.001200882	0.007168330	0.013986644	0.049986706	0.06231051	0.008955463	0.04957766	0.008882036	0.05585127	0.010005979	1.2540990	0.046178428	0.06410236	0.003516878	0.124687842	18	35	2047	7.85	656	2226	1903	121	22.5	21	52.5	27	7.73	29.25	856	853	1931	86	2170	1.33	2.06
Thalassiosira pseudonana (phytoplankton)	172.4	pCO2=750 ppm	1.9427310	3	19.72	4.126	56654078722	0.09220	0.004387436	0.0131000	0.000911680	0.005299116	0.010721184	0.037948668	0.04295175	0.006228660	0.08336780	0.015069952	0.08247768	0.014909049	2.1792940	0.080967390	0.07575479	0.004193536	0.149707321	18	35	2024	7.82	706	2186	1889	111	24.2	21	52.5	27	7.70	31.05	909	906	1913	80	2136	1.23	1.91
Thalassiosira pseudonana (phytoplankton)	31.5	pCO2=750 ppm	1.3510400	1	20.40	6.478	61241692520	0.19280	0.008487326	0.0285200	0.001836135	0.010323461	0.023341081	0.076429103	0.05678840	0.011961044	0.10853750	0.028496338	0.05481395	0.014391310	1.1146050	0.060146591	0.04416144	0.003550661	0.190907199	18	35	2007	7.92	551	2213	1850	138	18.9	21	52.5	27	7.80	24.28	711	708	1884	99	2155	1.53	2.36
Thalassiosira pseudonana (phytoplankton)	81.3	pCO2=750 ppm	1.9518000	2	22.58	5.467	65470039820	0.14170	0.005834965	0.0370300	0.002230043	0.008065009	0.030305758	0.092825551	0.05929470	0.009859118	0.06085745	0.012613502	0.10702460	0.022182248	1.3272730	0.056540937	0.05577361	0.003540034	0.183444151	18	35	2047	7.85	656	2226	1903	121	22.5	21	52.5	27	7.73	29.25	856	853	1931	86	2170	1.33	2.06
Thalassiosira pseudonana (phytoplankton)	168.7	pCO2=750 ppm	2.3091600	3	25.00	3.770	54709147360	0.10020	0.004937633	0.0100400	0.000723562	0.005661195	0.008216846	0.030118282	0.05576332	0.007651485	0.08312297	0.014217326	0.09015035	0.015419287	1.3906530	0.048887345	0.08629127	0.004519813	0.111055447	18	35	2024	7.82	706	2186	1889	111	24.2	21	52.5	27	7.70	31.05	909	906	1913	80	2136	1.23	1.91
Thalassiosira pseudonana (phytoplankton)	29.4	pCO2=750 ppm	1.2620570	1	18.41	8.934	66370148160	0.25870	0.010508354	0.0698900	0.004151877	0.014660230	0.057198743	0.172821863	0.06018591	0.016131833	0.03097592	0.010349345	0.06892685	0.023029106	1.4453420	0.099252181	0.03687942	0.003773371	0.316988822	18	35	2007	7.92	551	2213	1850	138	18.9	21	52.5	27	7.80	24.28	711	708	1884	99	2155	1.53	2.36
Thalassiosira pseudonana (phytoplankton)	78.3	pCO2=750 ppm	1.3702800	2	22.58	7.416	65807512700	0.19860	0.008136073	0.0199100	0.001192883	0.009328956	0.016294562	0.049653768	0.04092976	0.009184365	0.05737848	0.016049399	0.05400099	0.015104677	1.4417660	0.082886786	0.03996683	0.003423467	0.170526741	18	35	2047	7.85	656	2226	1903	121	22.5	21	52.5	27	7.73	29.25	856	853	1931	86	2170	1.33	2.06
Thalassiosira pseudonana (phytoplankton)	161.1	pCO2=750 ppm	2.2128900	3	25.88	5.836	60658702700	0.15160	0.006737785	0.0000000	0.000000000	0.006737785	0.000000000	0.000000000	0.04148204	0.007946904	0.07000579	0.016717510	0.08282661	0.019779146	2.2864240	0.112221278	0.06295320	0.004603755	0.148227232	18	35	2024	7.82	706	2186	1889	111	24.2	21	52.5	27	7.70	31.05	909	906	1913	80	2136	1.23	1.91
Thalassiosira pseudonana (phytoplankton)	236.5	pCO2=750 ppm	1.4513700	1	26.78	4.371	109485000000	0.10400	0.002560883	0.0076470	0.000275384	0.002836267	0.006258389	0.011462858	0.03419657	0.002718469	0.11515550	0.011411069	0.06772665	0.006711217	1.7901580	0.036459761	0.07445756	0.002259473	0.067147898	18	35	2064	7.86	639	2282	1916	126	22.0	21	52.5	27	7.74	28.80	843	840	1946	89	2191	1.37	2.13
Thalassiosira pseudonana (phytoplankton)	250.3	pCO2=750 ppm	1.4205600	2	21.09	3.674	91265216600	0.07270	0.002147535	0.0040970	0.000176996	0.002324530	0.003353030	0.007367448	0.04568393	0.003661955	0.13428670	0.013417832	0.08730416	0.008723370	2.6104530	0.053610026	0.06940476	0.002123711	0.082505502	18	35	2064	7.86	639	2282	1916	126	22.0	21	52.5	27	7.74	28.80	843	840	1946	89	2191	1.37	2.13
Thalassiosira pseudonana (phytoplankton)	248.8	pCO2=750 ppm	1.6149230	3	26.78	3.947	48729560306	0.07621	0.004216290	0.0093600	0.000757331	0.004973621	0.007660327	0.031523889	0.03287249	0.005301795	0.08677792	0.017446140	0.06836806	0.013744956	1.8887360	0.078044475	0.06069231	0.003736634	0.141026554	18	35	2064	7.86	639	2282	1916	126	22.0	21	52.5	27	7.74	28.80	843	840	1946	89	2191	1.37	2.13
Thalassiosira pseudonana (phytoplankton)	370.5	pCO2=750 ppm	1.3097140	1	28.66	5.084	33285761920	0.09508	0.007700904	0.0062090	0.000735471	0.008436375	0.005081514	0.030613965	0.02768752	0.008420673	0.09763750	0.037015121	0.05102043	0.019342234	2.4313950	0.189451842	0.07181905	0.008337943	0.282275919	18	35	2152	7.82	745	2320	2007	119	25.5	21	52.5	27	7.70	33.01	966	963	2034	85	2267	1.31	2.03
Thalassiosira pseudonana (phytoplankton)	394.4	pCO2=750 ppm	1.0887360	2	28.66	6.082	41091574672	0.10580	0.006941350	0.0128000	0.001228172	0.008169522	0.010475660	0.051122680	0.03305854	0.009743007	0.08979989	0.032990197	0.07008606	0.025747837	2.0665350	0.156038825	0.07124689	0.008015521	0.266079752	18	35	2152	7.82	745	2320	2007	119	25.5	21	52.5	27	7.70	33.01	966	963	2034	85	2267	1.31	2.03
Thalassiosira pseudonana (phytoplankton)	387.7	pCO2=750 ppm	1.1133300	3	29.65	6.270	73453585880	0.09483	0.003480517	0.0181400	0.000973703	0.004454220	0.014845975	0.040530383	0.02765822	0.004701046	0.11663600	0.024711701	0.07191153	0.015235915	1.9743430	0.085975244	0.07228478	0.004690008	0.165062602	18	35	2152	7.82	745	2320	2007	119	25.5	21	52.5	27	7.70	33.01	966	963	2034	85	2267	1.31	2.03