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Knapp, Jarred L; Bridges, Christopher R; Krohn, Janina; Hoffman, Louwrens C; Auerswald, Lutz (2015): Acid-base balance and changes in haemolymph properties of the South African rock lobsters, Jasus lalandii, a palinurid decapod, during chronic hypercapnia. PANGAEA, https://doi.org/10.1594/PANGAEA.847479, Supplement to: Knapp, JL et al. (2015): Acid-base balance and changes in haemolymph properties of the South African rock lobsters, Jasus lalandii, a palinurid decapod, during chronic hypercapnia. Biochemical and Biophysical Research Communications, 461(3), 475-480, https://doi.org/10.1016/j.bbrc.2015.04.025

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Abstract:
Few studies exist reporting on long-term exposure of crustaceans to hypercapnia. We exposed juvenile South African rock lobsters, Jasus lalandii, to hypercapnic conditions of pH 7.3 for 28 weeks and subsequently analysed changes in the extracellular fluid (haemolymph). Results revealed, for the first time, adjustments in the haemolymph of a palinurid crustacean during chronic hypercapnic exposure: 1) acid-base balance was adjusted and sustained by increased bicarbonate and 2) quantity and oxygen binding properties of haemocyanin changed. Compared with lobsters kept under normocapnic conditions (pH 8.0), during prolonged hypercapnia, juvenile lobsters increased bicarbonate buffering of haemolymph. This is necessary to provide optimum pH conditions for oxygen binding of haemocyanin and functioning of respiration in the presence of a strong Bohr Effect. Furthermore, modification of the intrinsic structure of the haemocyanin molecule, and not the presence of molecular modulators, seems to improve oxygen affinity under conditions of elevated pCO2.
Keyword(s):
Acid-base regulation; Animalia; Arthropoda; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20- 1000 L or < 1 m**2); Jasus lalandii; Laboratory experiment; Single species; South Atlantic; Temperate
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloise (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.6. https://cran.r-project.org/package=seacarb
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-06-01.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1SpeciesSpeciesKnapp, Jarred L
2FigureFigKnapp, Jarred L
3TreatmentTreatKnapp, Jarred L
4Carbon dioxideCO2%Knapp, Jarred L
5Hill coefficientHill coeffKnapp, Jarred Lat 50% Haemocyanin saturation (n50)
6pHpHKnapp, Jarred LPotentiometricNBS scale
7Haemolymph, partial pressure of oxygenpO2 (ha)TorrKnapp, Jarred Llog (p50)
8Haemolymph, partial pressure of oxygenpO2 (ha)TorrKnapp, Jarred Lat 50% Haemocyanin saturation (p50)
9Bohr CoefficientBohr coeffKnapp, Jarred L
10SalinitySalKnapp, Jarred L
11Salinity, standard errorSal std e±Knapp, Jarred L
12Temperature, waterTemp°CKnapp, Jarred L
13Temperature, water, standard errorT std e±Knapp, Jarred L
14pHpHKnapp, Jarred LPotentiometricaverage, NBS scale
15pH, standard errorpH std e±Knapp, Jarred LPotentiometricNBS scale
16Alkalinity, totalATµmol/kgKnapp, Jarred L
17Alkalinity, total, standard errorAT std e±Knapp, Jarred L
18Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetTorrKnapp, Jarred LCalculated using CO2SYS
19Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard errorpCO2water_SST_wet std e±Knapp, Jarred LCalculated using CO2SYS
20Bicarbonate[HCO3]-mmol/lKnapp, Jarred LCalculated using CO2SYS
21Bicarbonate ion, standard error[HCO3]- std e±Knapp, Jarred LCalculated using CO2SYS
22Carbonate ion[CO3]2-µmol/lKnapp, Jarred LCalculated using CO2SYS
23Carbonate ion, standard error[CO3]2- std e±Knapp, Jarred LCalculated using CO2SYS
24Haemolymph, pHpH (ha)Knapp, Jarred LNBS scale
25Haemolymph, pH, standard errorpH (ha) std e±Knapp, Jarred LNBS scale
26Haemolymph, total carbon dioxideTCO2 (ha)mmol/lKnapp, Jarred L
27Haemolymph, total carbon dioxide, standard errorTCO2 (ha) std e±Knapp, Jarred L
28Haemolymph, partial pressure of carbon dioxidepCO2 (ha)TorrKnapp, Jarred L
29Haemolymph, partial pressure of carbon dioxide, standard errorpCO2 (ha) std e±Knapp, Jarred L
30Haemolymph, bicarbonate ion[HCO3]- (ha)mmol/lKnapp, Jarred L
31Haemolymph, bicarbonate ion, standard error[HCO3]- (ha) std e±Knapp, Jarred L
32Haemolymph, calcium ionCa2+ (ha)mmol/lKnapp, Jarred L
33Haemolymph, calcium ion, standard errorCa2+ (ha) std e±Knapp, Jarred L
34Haemolymph, magnesium ionMg2+ (ha)mmol/lKnapp, Jarred L
35Haemolymph, magnesium ion, standard errorMg2+ (ha) std e±Knapp, Jarred L
36Haemolymph, lactateLactate (ha)mmol/lKnapp, Jarred L
37Haemolymph, lactate, standard errorLactate (ha) std e±Knapp, Jarred L
38Haemolymph, haemocyaninHaemocyanin (ha)mg/mlKnapp, Jarred L
39Haemolymph, haemocyanin, standard errorHaemocyanin (ha) std e±Knapp, Jarred L
40Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
41pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
42Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
43Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
44Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
45Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
46Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
47Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
48Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
49Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Size:
1316 data points

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