Citation:

j.bbrc.2015.04.025

Name: Acid-base balance and changes in haemolymph properties of the South African rock lobsters, Jasus lalandii, a palinurid decapod, during chronic hypercapnia
Published: 2015-06-25
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Acid-base regulation; Animalia; Arthropoda; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Jasus lalandii; Laboratory experiment; Single species; South Atlantic; Temperate

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Abstract:

Few studies exist reporting on long-term exposure of crustaceans to hypercapnia. We exposed juvenile South African rock lobsters, Jasus lalandii, to hypercapnic conditions of pH 7.3 for 28 weeks and subsequently analysed changes in the extracellular fluid (haemolymph). Results revealed, for the first time, adjustments in the haemolymph of a palinurid crustacean during chronic hypercapnic exposure: 1) acid-base balance was adjusted and sustained by increased bicarbonate and 2) quantity and oxygen binding properties of haemocyanin changed. Compared with lobsters kept under normocapnic conditions (pH 8.0), during prolonged hypercapnia, juvenile lobsters increased bicarbonate buffering of haemolymph. This is necessary to provide optimum pH conditions for oxygen binding of haemocyanin and functioning of respiration in the presence of a strong Bohr Effect. Furthermore, modification of the intrinsic structure of the haemocyanin molecule, and not the presence of molecular modulators, seems to improve oxygen affinity under conditions of elevated pCO2.

Keyword(s):

Acid-base regulation; Animalia; Arthropoda; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Jasus lalandii; Laboratory experiment; Single species; South Atlantic; Temperate

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse (2015): seacarb: seawater carbonate chemistry with R. R package version 3.0.6. https://cran.r-project.org/package=seacarb

Project(s):

Biological Impacts of Ocean Acidification (BIOACID)

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-06-01.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Knapp, Jarred L
2	Figure	Fig		Knapp, Jarred L
3	Treatment	Treat		Knapp, Jarred L
4	Carbon dioxide	CO2	%	Knapp, Jarred L
5	Hill coefficient	Hill coeff		Knapp, Jarred L		at 50% Haemocyanin saturation (n50)
6	pH	pH		Knapp, Jarred L	Potentiometric	NBS scale
7	Haemolymph, partial pressure of oxygen	pO2 (ha)	Torr	Knapp, Jarred L		log (p50)
8	Haemolymph, partial pressure of oxygen	pO2 (ha)	Torr	Knapp, Jarred L		at 50% Haemocyanin saturation (p50)
9	Bohr Coefficient	Bohr coeff		Knapp, Jarred L
10	Salinity	Sal		Knapp, Jarred L
11	Salinity, standard error	Sal std e	±	Knapp, Jarred L
12	Temperature, water	Temp	°C	Knapp, Jarred L
13	Temperature, water, standard error	T std e	±	Knapp, Jarred L
14	pH	pH		Knapp, Jarred L	Potentiometric	average, NBS scale
15	pH, standard error	pH std e	±	Knapp, Jarred L	Potentiometric	NBS scale
16	Alkalinity, total	AT	µmol/kg	Knapp, Jarred L
17	Alkalinity, total, standard error	AT std e	±	Knapp, Jarred L
18	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	Torr	Knapp, Jarred L	Calculated using CO2SYS
19	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error	pCO2water_SST_wet std e	±	Knapp, Jarred L	Calculated using CO2SYS
20	Bicarbonate	[HCO3]-	mmol/l	Knapp, Jarred L	Calculated using CO2SYS
21	Bicarbonate ion, standard error	[HCO3]- std e	±	Knapp, Jarred L	Calculated using CO2SYS
22	Carbonate ion	[CO3]2-	µmol/l	Knapp, Jarred L	Calculated using CO2SYS
23	Carbonate ion, standard error	[CO3]2- std e	±	Knapp, Jarred L	Calculated using CO2SYS
24	Haemolymph, pH	pH (ha)		Knapp, Jarred L		NBS scale
25	Haemolymph, pH, standard error	pH (ha) std e	±	Knapp, Jarred L		NBS scale
26	Haemolymph, total carbon dioxide	TCO2 (ha)	mmol/l	Knapp, Jarred L
27	Haemolymph, total carbon dioxide, standard error	TCO2 (ha) std e	±	Knapp, Jarred L
28	Haemolymph, partial pressure of carbon dioxide	pCO2 (ha)	Torr	Knapp, Jarred L
29	Haemolymph, partial pressure of carbon dioxide, standard error	pCO2 (ha) std e	±	Knapp, Jarred L
30	Haemolymph, bicarbonate ion	[HCO3]- (ha)	mmol/l	Knapp, Jarred L
31	Haemolymph, bicarbonate ion, standard error	[HCO3]- (ha) std e	±	Knapp, Jarred L
32	Haemolymph, calcium ion	Ca2+ (ha)	mmol/l	Knapp, Jarred L
33	Haemolymph, calcium ion, standard error	Ca2+ (ha) std e	±	Knapp, Jarred L
34	Haemolymph, magnesium ion	Mg2+ (ha)	mmol/l	Knapp, Jarred L
35	Haemolymph, magnesium ion, standard error	Mg2+ (ha) std e	±	Knapp, Jarred L
36	Haemolymph, lactate	Lactate (ha)	mmol/l	Knapp, Jarred L
37	Haemolymph, lactate, standard error	Lactate (ha) std e	±	Knapp, Jarred L
38	Haemolymph, haemocyanin	Haemocyanin (ha)	mg/ml	Knapp, Jarred L
39	Haemolymph, haemocyanin, standard error	Haemocyanin (ha) std e	±	Knapp, Jarred L
40	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
42	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1316 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Species	2 Fig	3 Treat	4 CO2 [%]	5 Hill coeff (at 50% Haemocyanin saturation...)	6 pH (NBS scale, Potentiometric)	7 pO2 (ha) [Torr] (log (p50))	8 pO2 (ha) [Torr] (at 50% Haemocyanin saturation...)	9 Bohr coeff	10 Sal	11 Sal std e [±]	12 Temp [°C]	13 T std e [±]	14 pH (average, NBS scale, Potentiom...)	15 pH std e [±] (NBS scale, Potentiometric)	16 AT [µmol/kg]	17 AT std e [±]	18 pCO2water_SST_wet [Torr] (Calculated using CO2SYS)	19 pCO2water_SST_wet std e [±] (Calculated using CO2SYS)	20 [HCO3]- [mmol/l] (Calculated using CO2SYS)	21 [HCO3]- std e [±] (Calculated using CO2SYS)	22 [CO3]2- [µmol/l] (Calculated using CO2SYS)	23 [CO3]2- std e [±] (Calculated using CO2SYS)	24 pH (ha) (NBS scale)	25 pH (ha) std e [±] (NBS scale)	26 TCO2 (ha) [mmol/l]	27 TCO2 (ha) std e [±]	28 pCO2 (ha) [Torr]	29 pCO2 (ha) std e [±]	30 [HCO3]- (ha) [mmol/l]	31 [HCO3]- (ha) std e [±]	32 Ca2+ (ha) [mmol/l]	33 Ca2+ (ha) std e [±]	34 Mg2+ (ha) [mmol/l]	35 Mg2+ (ha) std e [±]	36 Lactate (ha) [mmol/l]	37 Lactate (ha) std e [±]	38 Haemocyanin (ha) [mg/ml]	39 Haemocyanin (ha) std e [±]	40 CSC flag (Calculated using seacarb afte...)	41 pH (total scale, Calculated using...)	42 CO2 [µmol/kg] (Calculated using seacarb afte...)	43 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	44 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	45 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	46 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	47 DIC [µmol/kg] (Calculated using seacarb afte...)	48 Omega Arg (Calculated using seacarb afte...)	49 Omega Cal (Calculated using seacarb afte...)
Jasus lalandii (crustacean)	2	Normocapnia whole blood	0.0	3.3813	7.987	1.16	14.48	-0.42	34.4	0.1	18.8	0.1	8.02	0.02	1903	8	0.27	0.01	1.58	0.02	140	0.01	7.760	0.025	4.2	0.4	1.7	0.2	4.1	0.4	12.8	0.8	17.5	0.6	0.4	0.1	22.3	2.0	26	7.9	16.52	491.44	493.14	1624.54	108.62	1749.69	1.69	2.61
Jasus lalandii (crustacean)	2	Normocapnia whole blood	0.3	2.7109	7.519	1.40	25.36		34.4	0.1	18.8	0.1	8.02	0.02	1903	8	0.27	0.01	1.58	0.02	140	0.01	7.760	0.025	4.2	0.4	1.7	0.2	4.1	0.4	12.8	0.8	17.5	0.6	0.4	0.1	22.3	2.0	26	7.9	16.52	491.44	493.14	1624.54	108.62	1749.69	1.69	2.61
Jasus lalandii (crustacean)	2	Normocapnia whole blood	0.5	3.4627	7.451	1.40	25.36		34.4	0.1	18.8	0.1	8.02	0.02	1903	8	0.27	0.01	1.58	0.02	140	0.01	7.760	0.025	4.2	0.4	1.7	0.2	4.1	0.4	12.8	0.8	17.5	0.6	0.4	0.1	22.3	2.0	26	7.9	16.52	491.44	493.14	1624.54	108.62	1749.69	1.69	2.61
Jasus lalandii (crustacean)	2	Normocapnia whole blood	0.7	3.1730	7.343	1.42	26.27		34.4	0.1	18.8	0.1	8.02	0.02	1903	8	0.27	0.01	1.58	0.02	140	0.01	7.760	0.025	4.2	0.4	1.7	0.2	4.1	0.4	12.8	0.8	17.5	0.6	0.4	0.1	22.3	2.0	26	7.9	16.52	491.44	493.14	1624.54	108.62	1749.69	1.69	2.61
Jasus lalandii (crustacean)	2	Normocapnia whole blood	1.0	2.9098	7.232	1.49	30.63		34.4	0.1	18.8	0.1	8.02	0.02	1903	8	0.27	0.01	1.58	0.02	140	0.01	7.760	0.025	4.2	0.4	1.7	0.2	4.1	0.4	12.8	0.8	17.5	0.6	0.4	0.1	22.3	2.0	26	7.9	16.52	491.44	493.14	1624.54	108.62	1749.69	1.69	2.61
Jasus lalandii (crustacean)	2	Hypercapnia whole blood	0.0	3.2227	8.337	0.81	6.49	-0.66	34.4	0.0	18.8	0.1	7.32	0.01	1925	7	1.57	0.03	1.88	0.01	40	0.00	7.655	0.061	8.5	1.2	3.9	0.2	8.4	1.2	9.2	0.6	19.4	1.5	0.3	0.1	13.9	2.1	26	7.2	95.01	2825.48	2835.27	1861.75	24.81	1981.57	0.39	0.60
Jasus lalandii (crustacean)	2	Hypercapnia whole blood	0.3	3.3477	7.838	1.25	17.79		34.4	0.0	18.8	0.1	7.32	0.01	1925	7	1.57	0.03	1.88	0.01	40	0.00	7.655	0.061	8.5	1.2	3.9	0.2	8.4	1.2	9.2	0.6	19.4	1.5	0.3	0.1	13.9	2.1	26	7.2	95.01	2825.48	2835.27	1861.75	24.81	1981.57	0.39	0.60
Jasus lalandii (crustacean)	2	Hypercapnia whole blood	0.5	2.6310	7.643	1.37	23.48		34.4	0.0	18.8	0.1	7.32	0.01	1925	7	1.57	0.03	1.88	0.01	40	0.00	7.655	0.061	8.5	1.2	3.9	0.2	8.4	1.2	9.2	0.6	19.4	1.5	0.3	0.1	13.9	2.1	26	7.2	95.01	2825.48	2835.27	1861.75	24.81	1981.57	0.39	0.60
Jasus lalandii (crustacean)	2	Hypercapnia whole blood	0.7						34.4	0.0	18.8	0.1	7.32	0.01	1925	7	1.57	0.03	1.88	0.01	40	0.00	7.655	0.061	8.5	1.2	3.9	0.2	8.4	1.2	9.2	0.6	19.4	1.5	0.3	0.1	13.9	2.1	26	7.2	95.01	2825.48	2835.27	1861.75	24.81	1981.57	0.39	0.60
Jasus lalandii (crustacean)	2	Hypercapnia whole blood	1.0	3.3618	7.321	1.46	28.78		34.4	0.0	18.8	0.1	7.32	0.01	1925	7	1.57	0.03	1.88	0.01	40	0.00	7.655	0.061	8.5	1.2	3.9	0.2	8.4	1.2	9.2	0.6	19.4	1.5	0.3	0.1	13.9	2.1	26	7.2	95.01	2825.48	2835.27	1861.75	24.81	1981.57	0.39	0.60
Jasus lalandii (crustacean)	2	Normocapnia dialysed	0.0	4.1466	8.255	0.98	9.65	-0.57	34.4	0.1	18.8	0.1	8.02	0.02	1903	8	0.27	0.01	1.58	0.02	140	0.01	7.760	0.025	4.2	0.4	1.7	0.2	4.1	0.4	12.8	0.8	17.5	0.6	0.4	0.1	22.3	2.0	26	7.9	16.52	491.44	493.14	1624.54	108.62	1749.69	1.69	2.61
Jasus lalandii (crustacean)	2	Normocapnia dialysed	0.3	3.3297	7.569	1.40	25.38		34.4	0.1	18.8	0.1	8.02	0.02	1903	8	0.27	0.01	1.58	0.02	140	0.01	7.760	0.025	4.2	0.4	1.7	0.2	4.1	0.4	12.8	0.8	17.5	0.6	0.4	0.1	22.3	2.0	26	7.9	16.52	491.44	493.14	1624.54	108.62	1749.69	1.69	2.61
Jasus lalandii (crustacean)	2	Normocapnia dialysed	0.5	3.2605	7.486	1.43	26.77		34.4	0.1	18.8	0.1	8.02	0.02	1903	8	0.27	0.01	1.58	0.02	140	0.01	7.760	0.025	4.2	0.4	1.7	0.2	4.1	0.4	12.8	0.8	17.5	0.6	0.4	0.1	22.3	2.0	26	7.9	16.52	491.44	493.14	1624.54	108.62	1749.69	1.69	2.61
Jasus lalandii (crustacean)	2	Normocapnia dialysed	0.7	4.6535	7.386	1.47	29.20		34.4	0.1	18.8	0.1	8.02	0.02	1903	8	0.27	0.01	1.58	0.02	140	0.01	7.760	0.025	4.2	0.4	1.7	0.2	4.1	0.4	12.8	0.8	17.5	0.6	0.4	0.1	22.3	2.0	26	7.9	16.52	491.44	493.14	1624.54	108.62	1749.69	1.69	2.61
Jasus lalandii (crustacean)	2	Normocapnia dialysed	1.0						34.4	0.1	18.8	0.1	8.02	0.02	1903	8	0.27	0.01	1.58	0.02	140	0.01	7.760	0.025	4.2	0.4	1.7	0.2	4.1	0.4	12.8	0.8	17.5	0.6	0.4	0.1	22.3	2.0	26	7.9	16.52	491.44	493.14	1624.54	108.62	1749.69	1.69	2.61
Jasus lalandii (crustacean)	2	Hypercapnia dialysed	0.0	3.9206	7.995	0.89	7.84	-0.62	34.4	0.0	18.8	0.1	7.32	0.01	1925	7	1.57	0.03	1.88	0.01	40	0.00	7.655	0.061	8.5	1.2	3.9	0.2	8.4	1.2	9.2	0.6	19.4	1.5	0.3	0.1	13.9	2.1	26	7.2	95.01	2825.48	2835.27	1861.75	24.81	1981.57	0.39	0.60
Jasus lalandii (crustacean)	2	Hypercapnia dialysed	0.3	2.8747	7.564	1.28	19.07		34.4	0.0	18.8	0.1	7.32	0.01	1925	7	1.57	0.03	1.88	0.01	40	0.00	7.655	0.061	8.5	1.2	3.9	0.2	8.4	1.2	9.2	0.6	19.4	1.5	0.3	0.1	13.9	2.1	26	7.2	95.01	2825.48	2835.27	1861.75	24.81	1981.57	0.39	0.60
Jasus lalandii (crustacean)	2	Hypercapnia dialysed	0.5	3.0980	7.480	1.42	26.30		34.4	0.0	18.8	0.1	7.32	0.01	1925	7	1.57	0.03	1.88	0.01	40	0.00	7.655	0.061	8.5	1.2	3.9	0.2	8.4	1.2	9.2	0.6	19.4	1.5	0.3	0.1	13.9	2.1	26	7.2	95.01	2825.48	2835.27	1861.75	24.81	1981.57	0.39	0.60
Jasus lalandii (crustacean)	2	Hypercapnia dialysed	0.7	3.1144	7.166	1.42	26.50		34.4	0.0	18.8	0.1	7.32	0.01	1925	7	1.57	0.03	1.88	0.01	40	0.00	7.655	0.061	8.5	1.2	3.9	0.2	8.4	1.2	9.2	0.6	19.4	1.5	0.3	0.1	13.9	2.1	26	7.2	95.01	2825.48	2835.27	1861.75	24.81	1981.57	0.39	0.60
Jasus lalandii (crustacean)	2	Hypercapnia dialysed	1.0	4.4739	7.114	1.48	29.95		34.4	0.0	18.8	0.1	7.32	0.01	1925	7	1.57	0.03	1.88	0.01	40	0.00	7.655	0.061	8.5	1.2	3.9	0.2	8.4	1.2	9.2	0.6	19.4	1.5	0.3	0.1	13.9	2.1	26	7.2	95.01	2825.48	2835.27	1861.75	24.81	1981.57	0.39	0.60
Jasus lalandii (crustacean)	3	Whole Normocapnic			7.650		21.23		34.4	0.1	18.8	0.1	8.02	0.02	1903	8	0.27	0.01	1.58	0.02	140	0.01	7.760	0.025	4.2	0.4	1.7	0.2	4.1	0.4	12.8	0.8	17.5	0.6	0.4	0.1	22.3	2.0	26	7.9	16.52	491.44	493.14	1624.54	108.62	1749.69	1.69	2.61
Jasus lalandii (crustacean)	3	Whole Normocapnic			7.760		19.09		34.4	0.1	18.8	0.1	8.02	0.02	1903	8	0.27	0.01	1.58	0.02	140	0.01	7.760	0.025	4.2	0.4	1.7	0.2	4.1	0.4	12.8	0.8	17.5	0.6	0.4	0.1	22.3	2.0	26	7.9	16.52	491.44	493.14	1624.54	108.62	1749.69	1.69	2.61
Jasus lalandii (crustacean)	3	Whole Hypercapnic			7.650		18.66		34.4	0.0	18.8	0.1	7.32	0.01	1925	7	1.57	0.03	1.88	0.01	40	0.00	7.655	0.061	8.5	1.2	3.9	0.2	8.4	1.2	9.2	0.6	19.4	1.5	0.3	0.1	13.9	2.1	26	7.2	95.01	2825.48	2835.27	1861.75	24.81	1981.57	0.39	0.60
Jasus lalandii (crustacean)	3	Whole Hypercapnic			7.760		15.79		34.4	0.0	18.8	0.1	7.32	0.01	1925	7	1.57	0.03	1.88	0.01	40	0.00	7.655	0.061	8.5	1.2	3.9	0.2	8.4	1.2	9.2	0.6	19.4	1.5	0.3	0.1	13.9	2.1	26	7.2	95.01	2825.48	2835.27	1861.75	24.81	1981.57	0.39	0.60
Jasus lalandii (crustacean)	3	Dialysed Normocapnic			7.650		21.36		34.4	0.1	18.8	0.1	8.02	0.02	1903	8	0.27	0.01	1.58	0.02	140	0.01	7.760	0.025	4.2	0.4	1.7	0.2	4.1	0.4	12.8	0.8	17.5	0.6	0.4	0.1	22.3	2.0	26	7.9	16.52	491.44	493.14	1624.54	108.62	1749.69	1.69	2.61
Jasus lalandii (crustacean)	3	Dialysed Normocapnic			7.760		18.48		34.4	0.1	18.8	0.1	8.02	0.02	1903	8	0.27	0.01	1.58	0.02	140	0.01	7.760	0.025	4.2	0.4	1.7	0.2	4.1	0.4	12.8	0.8	17.5	0.6	0.4	0.1	22.3	2.0	26	7.9	16.52	491.44	493.14	1624.54	108.62	1749.69	1.69	2.61
Jasus lalandii (crustacean)	3	Dialysed hypercapnic			7.650		16.11		34.4	0.0	18.8	0.1	7.32	0.01	1925	7	1.57	0.03	1.88	0.01	40	0.00	7.655	0.061	8.5	1.2	3.9	0.2	8.4	1.2	9.2	0.6	19.4	1.5	0.3	0.1	13.9	2.1	26	7.2	95.01	2825.48	2835.27	1861.75	24.81	1981.57	0.39	0.60
Jasus lalandii (crustacean)	3	Dialysed hypercapnic			7.760		13.77		34.4	0.0	18.8	0.1	7.32	0.01	1925	7	1.57	0.03	1.88	0.01	40	0.00	7.655	0.061	8.5	1.2	3.9	0.2	8.4	1.2	9.2	0.6	19.4	1.5	0.3	0.1	13.9	2.1	26	7.2	95.01	2825.48	2835.27	1861.75	24.81	1981.57	0.39	0.60