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Data Publisher for Earth & Environmental Science

Bresolin de Souza, Karine; Jutfelt, Fredrik; Kling, Peter; Förlin, Lars; Sturve, Joachim; Hofmann, Gretchen E (2014): Effects of increased CO2 on fish gill and plasma proteome [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.838003, Supplement to: Bresolin de Souza, K et al. (2014): Effects of Increased CO2 on Fish Gill and Plasma Proteome. PLoS ONE, 9(7), e102901, https://doi.org/10.1371/journal.pone.0102901

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Abstract:
Ocean acidification and warming are both primarily caused by increased levels of atmospheric CO2, and marine organisms are exposed to these two stressors simultaneously. Although the effects of temperature on fish have been investigated over the last century, the long-term effects of moderate CO2 exposure and the combination of both stressors are almost entirely unknown. A proteomics approach was used to assess the adverse physiological and biochemical changes that may occur from the exposure to these two environmental stressors. We analysed gills and blood plasma of Atlantic halibut (Hippoglossus hippoglossus) exposed to temperatures of 12°C (control) and 18°C (impaired growth) in combination with control (400 µatm) or high-CO2 water (1000 µatm) for 14 weeks. The proteomic analysis was performed using two-dimensional gel electrophoresis (2DE) followed by Nanoflow LC-MS/MS using a LTQ-Orbitrap. The high-CO2 treatment induced the up-regulation of immune system-related proteins, as indicated by the up-regulation of the plasma proteins complement component C3 and fibrinogen beta chain precursor in both temperature treatments. Changes in gill proteome in the high-CO2 (18°C) group were mostly related to increased energy metabolism proteins (ATP synthase, malate dehydrogenase, malate dehydrogenase thermostable, and fructose-1,6-bisphosphate aldolase), possibly coupled to a higher energy demand. Gills from fish exposed to high-CO2 at both temperature treatments showed changes in proteins associated with increased cellular turnover and apoptosis signalling (annexin 5, eukaryotic translation elongation factor 1 gamma, receptor for protein kinase C, and putative ribosomal protein S27). This study indicates that moderate CO2-driven acidification, alone and combined with high temperature, can elicit biochemical changes that may affect fish health.
Keyword(s):
Acanthopagrus schlegelii; Acipenser baerii; Animalia; Anoplopoma fimbria; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Coturnix coturnix; Danio rerio; Dicentrarchus labrax; Epinephelus bruneus; Gene expression (incl. proteomics); Gillichthys mirabilis; Hippoglossus hippoglossus; Laboratory experiment; Larimichthys crocea; Nekton; North Atlantic; Oncorhynchus mykiss; Oreochromis mossambicus; Paralichthys olivaceus; Pelagos; Platichthys flesus; Pseudopleuronectes americanus; Salmo salar; Salmo trutta; Single species; Sphoeroides nephelus; Sphyraena idiastes; Takifugu rubripes; Temperate; Temperature
Further details:
Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0 [webpage]. https://cran.r-project.org/package=seacarb
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-11-06.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1SpeciesSpeciesBresolin de Souza, Karine
2TableTabBresolin de Souza, Karine
3IdentificationIDBresolin de Souza, Karinematchset
4Protein spots, totalProtein spots#Bresolin de Souza, Karinedetected
5Protein spots, total, standard deviationProtein spots std dev±Bresolin de Souza, Karinedetected
6Protein spots, totalProtein spots#Bresolin de Souza, Karinesignificantly different from control
7Protein spots, totalProtein spots#Bresolin de Souza, Karineidentified
8IdentificationIDBresolin de Souza, Karinespot
9CommentCommentBresolin de Souza, KarineSpots classified as "up" regulated are those containing increased amounts of protein; spots "down" regulated are those with reduced amounts of protein; spots classified as "high-CO2" are those detected only in the high-CO2 group (but not in the control group).
10Protein nameProteinBresolin de Souza, Karine
11SpeciesSpeciesBresolin de Souza, Karine
12CommentCommentBresolin de Souza, Karineproposed function
13PeptidePep#Bresolin de Souza, Karine
14Accession numberAccess NoBresolin de Souza, Karine
15ScoreScoreBresolin de Souza, Karine
16TreatmentTreatBresolin de Souza, Karine
17SalinitySalBresolin de Souza, Karine
18Salinity, standard errorSal std e±Bresolin de Souza, Karine
19Temperature, waterTemp°CBresolin de Souza, Karine
20Alkalinity, totalATµmol/kgBresolin de Souza, KarinePotentiometric titration
21pHpHBresolin de Souza, KarinePotentiometrictotal scale
22Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmBresolin de Souza, KarineCalculated
23Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
24Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
25Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
26Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
27Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
28Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
29Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
30Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
31Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
792 data points

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