Bresolin de Souza, Karine; Jutfelt, Fredrik; Kling, Peter; Förlin, Lars; Sturve, Joachim; Hofmann, Gretchen E (2014): Effects of increased CO2 on fish gill and plasma proteome [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.838003, Supplement to: Bresolin de Souza, K et al. (2014): Effects of Increased CO2 on Fish Gill and Plasma Proteome. PLoS ONE, 9(7), e102901, https://doi.org/10.1371/journal.pone.0102901
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Abstract:
Ocean acidification and warming are both primarily caused by increased levels of atmospheric CO2, and marine organisms are exposed to these two stressors simultaneously. Although the effects of temperature on fish have been investigated over the last century, the long-term effects of moderate CO2 exposure and the combination of both stressors are almost entirely unknown. A proteomics approach was used to assess the adverse physiological and biochemical changes that may occur from the exposure to these two environmental stressors. We analysed gills and blood plasma of Atlantic halibut (Hippoglossus hippoglossus) exposed to temperatures of 12°C (control) and 18°C (impaired growth) in combination with control (400 µatm) or high-CO2 water (1000 µatm) for 14 weeks. The proteomic analysis was performed using two-dimensional gel electrophoresis (2DE) followed by Nanoflow LC-MS/MS using a LTQ-Orbitrap. The high-CO2 treatment induced the up-regulation of immune system-related proteins, as indicated by the up-regulation of the plasma proteins complement component C3 and fibrinogen beta chain precursor in both temperature treatments. Changes in gill proteome in the high-CO2 (18°C) group were mostly related to increased energy metabolism proteins (ATP synthase, malate dehydrogenase, malate dehydrogenase thermostable, and fructose-1,6-bisphosphate aldolase), possibly coupled to a higher energy demand. Gills from fish exposed to high-CO2 at both temperature treatments showed changes in proteins associated with increased cellular turnover and apoptosis signalling (annexin 5, eukaryotic translation elongation factor 1 gamma, receptor for protein kinase C, and putative ribosomal protein S27). This study indicates that moderate CO2-driven acidification, alone and combined with high temperature, can elicit biochemical changes that may affect fish health.
Keyword(s):
Acanthopagrus schlegelii; Acipenser baerii; Animalia; Anoplopoma fimbria; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Coturnix coturnix; Danio rerio; Dicentrarchus labrax; Epinephelus bruneus; Gene expression (incl. proteomics); Gillichthys mirabilis; Hippoglossus hippoglossus; Laboratory experiment; Larimichthys crocea; Nekton; North Atlantic; Oncorhynchus mykiss; Oreochromis mossambicus; Paralichthys olivaceus; Pelagos; Platichthys flesus; Pseudopleuronectes americanus; Salmo salar; Salmo trutta; Single species; Sphoeroides nephelus; Sphyraena idiastes; Takifugu rubripes; Temperate; Temperature
Further details:
Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb
Project(s):
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-11-06.
Parameter(s):
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
792 data points
Data
1 Species | 2 Tab | 3 ID (matchset) | 4 Protein spots [#] (detected) | 5 Protein spots std dev [±] (detected) | 6 Protein spots [#] (significantly different from ...) | 7 Protein spots [#] (identified) | 8 ID (spot) | 9 Comment (Spots classified as "up" regu...) | 10 Protein | 11 Species | 12 Comment (proposed function) | 13 Pep [#] | 14 Access No | 15 Score | 16 Treat | 17 Sal | 18 Sal std e [±] | 19 Temp [°C] | 20 AT [µmol/kg] (Potentiometric titration) | 21 pH (total scale, Potentiometric) | 22 pCO2water_SST_wet [µatm] (Calculated) | 23 CSC flag (Calculated using seacarb afte...) | 24 CO2 [µmol/kg] (Calculated using seacarb afte...) | 25 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...) | 26 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...) | 27 [HCO3]- [µmol/kg] (Calculated using seacarb afte...) | 28 [CO3]2- [µmol/kg] (Calculated using seacarb afte...) | 29 DIC [µmol/kg] (Calculated using seacarb afte...) | 30 Omega Arg (Calculated using seacarb afte...) | 31 Omega Cal (Calculated using seacarb afte...) |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Hippoglossus hippoglossus (fish) | 2 | Gills 12 °C | 452 | 58 | 6 | 5 | Reduced pH | 32 | 0.14 | 11.71 | 2229.0 | 7.59 | 1302.5 | 8 | 52.46 | 1247.19 | 1242.55 | 2105.15 | 49.94 | 2207.55 | 0.77 | 1.21 | ||||||||
Hippoglossus hippoglossus (fish) | 2 | Gills 12 °C | 611 | 92 | 10 | 5 | Reduced pH | 32 | 0.14 | 17.88 | 2237.5 | 7.46 | 1826.0 | 8 | 60.86 | 1746.00 | 1740.00 | 2120.16 | 47.78 | 2228.80 | 0.75 | 1.16 | ||||||||
Hippoglossus hippoglossus (fish) | 2 | Plasma 12 °C | 455 | 67 | 26 | 6 | Reduced pH | 32 | 0.14 | 11.71 | 2229.0 | 7.59 | 1302.5 | 8 | 52.46 | 1247.19 | 1242.55 | 2105.15 | 49.94 | 2207.55 | 0.77 | 1.21 | ||||||||
Hippoglossus hippoglossus (fish) | 2 | Plasma 18 °C | 398 | 85 | 4 | 2 | Reduced pH | 32 | 0.14 | 17.88 | 2237.5 | 7.46 | 1826.0 | 8 | 60.86 | 1746.00 | 1740.00 | 2120.16 | 47.78 | 2228.80 | 0.75 | 1.16 | ||||||||
Hippoglossus hippoglossus (fish) | 3 | Gills 12 °C | 1 | Up | Annexin 5 | Anoplopoma fimbria | Apoptosis Signalling | 6 | 229366222 | 569 | Reduced pH | 32 | 0.14 | 11.71 | 2229.0 | 7.59 | 1302.5 | 8 | 52.46 | 1247.19 | 1242.55 | 2105.15 | 49.94 | 2207.55 | 0.77 | 1.21 | ||||
Hippoglossus hippoglossus (fish) | 3 | Gills 12 °C | 1 | Up | Annexin max1 | Epinephelus bruneus | Apoptosis Signalling | 2 | 328677117 | 337 | Reduced pH | 32 | 0.14 | 11.71 | 2229.0 | 7.59 | 1302.5 | 8 | 52.46 | 1247.19 | 1242.55 | 2105.15 | 49.94 | 2207.55 | 0.77 | 1.21 | ||||
Hippoglossus hippoglossus (fish) | 3 | Gills 12 °C | 1 | Up | Annexin 5B | Danio rerio | Apoptosis Signalling | 2 | 160773369 | 130 | Reduced pH | 32 | 0.14 | 11.71 | 2229.0 | 7.59 | 1302.5 | 8 | 52.46 | 1247.19 | 1242.55 | 2105.15 | 49.94 | 2207.55 | 0.77 | 1.21 | ||||
Hippoglossus hippoglossus (fish) | 3 | Gills 12 °C | 2 | Down | Cytoskeletal tropomyosin | Coturnix coturnix | Cellular Structure and Motility | 14 | 833603 | 3011 | Reduced pH | 32 | 0.14 | 11.71 | 2229.0 | 7.59 | 1302.5 | 8 | 52.46 | 1247.19 | 1242.55 | 2105.15 | 49.94 | 2207.55 | 0.77 | 1.21 | ||||
Hippoglossus hippoglossus (fish) | 3 | Gills 12 °C | 2 | Down | Tropomyosin 4-alpha | Takifugu rubripes | Cellular Structure and Motility | 11 | 28557136 | 1686 | Reduced pH | 32 | 0.14 | 11.71 | 2229.0 | 7.59 | 1302.5 | 8 | 52.46 | 1247.19 | 1242.55 | 2105.15 | 49.94 | 2207.55 | 0.77 | 1.21 | ||||
Hippoglossus hippoglossus (fish) | 3 | Gills 12 °C | 2 | Down | Tropomyosin 4-alpha | Salmo salar | Cellular Structure and Motility | 11 | 213515262 | 1575 | Reduced pH | 32 | 0.14 | 11.71 | 2229.0 | 7.59 | 1302.5 | 8 | 52.46 | 1247.19 | 1242.55 | 2105.15 | 49.94 | 2207.55 | 0.77 | 1.21 | ||||
Hippoglossus hippoglossus (fish) | 3 | Gills 12 °C | 3 | Down | Glyceraldehyde 3-phosphate dehydrogenase | Acanthopagrus schlegelii | Multifunctional/Energy Metabolism | 2 | 89147695 | 129 | Reduced pH | 32 | 0.14 | 11.71 | 2229.0 | 7.59 | 1302.5 | 8 | 52.46 | 1247.19 | 1242.55 | 2105.15 | 49.94 | 2207.55 | 0.77 | 1.21 | ||||
Hippoglossus hippoglossus (fish) | 3 | Gills 12 °C | 4 | Up | Enolase 1-alpha | Salmo trutta | Multifunctional/Energy Metabolism | 8 | 11999265 | 1061 | Reduced pH | 32 | 0.14 | 11.71 | 2229.0 | 7.59 | 1302.5 | 8 | 52.46 | 1247.19 | 1242.55 | 2105.15 | 49.94 | 2207.55 | 0.77 | 1.21 | ||||
Hippoglossus hippoglossus (fish) | 3 | Gills 12 °C | 4 | Up | Enolase 1-alpha | Gillichthys mirabilis | Multifunctional/Energy Metabolism | 9 | 226441951 | 775 | Reduced pH | 32 | 0.14 | 11.71 | 2229.0 | 7.59 | 1302.5 | 8 | 52.46 | 1247.19 | 1242.55 | 2105.15 | 49.94 | 2207.55 | 0.77 | 1.21 | ||||
Hippoglossus hippoglossus (fish) | 3 | Gills 12 °C | 4 | Up | Enolase-alpha | Acipenser baerii | Multifunctional/Energy Metabolism | 10 | 98979415 | 773 | Reduced pH | 32 | 0.14 | 11.71 | 2229.0 | 7.59 | 1302.5 | 8 | 52.46 | 1247.19 | 1242.55 | 2105.15 | 49.94 | 2207.55 | 0.77 | 1.21 | ||||
Hippoglossus hippoglossus (fish) | 3 | Gills 12 °C | 5 | High-CO2 | Eukaryotic translation elongation factor 1 gamma | Pseudopleuronectes americanus | Protein Biosynthesis/Apoptosis Related | 3 | 28394501 | 150 | Reduced pH | 32 | 0.14 | 11.71 | 2229.0 | 7.59 | 1302.5 | 8 | 52.46 | 1247.19 | 1242.55 | 2105.15 | 49.94 | 2207.55 | 0.77 | 1.21 | ||||
Hippoglossus hippoglossus (fish) | 3 | Gills 18 °C | 1 | Up | Fructose-1,6-bisphosphate aldolase | Sphoeroides nephelus | Energy Metabolism | 6 | 2828145 | 1698 | Reduced pH | 32 | 0.14 | 17.88 | 2237.5 | 7.46 | 1826.0 | 8 | 60.86 | 1746.00 | 1740.00 | 2120.16 | 47.78 | 2228.80 | 0.75 | 1.16 | ||||
Hippoglossus hippoglossus (fish) | 3 | Gills 18 °C | 2 | Up | Receptor for activated protein kinase C | Oreochromis mossambicus | Inflammation Mediator/Apoptosis Related | 9 | 37498964 | 1844 | Reduced pH | 32 | 0.14 | 17.88 | 2237.5 | 7.46 | 1826.0 | 8 | 60.86 | 1746.00 | 1740.00 | 2120.16 | 47.78 | 2228.80 | 0.75 | 1.16 | ||||
Hippoglossus hippoglossus (fish) | 3 | Gills 18 °C | 3 | Up | Malate dehydrogenase 1A | Danio rerio | Energy Metabolism | 7 | 41053939 | 1356 | Reduced pH | 32 | 0.14 | 17.88 | 2237.5 | 7.46 | 1826.0 | 8 | 60.86 | 1746.00 | 1740.00 | 2120.16 | 47.78 | 2228.80 | 0.75 | 1.16 | ||||
Hippoglossus hippoglossus (fish) | 3 | Gills 18 °C | 3 | Up | Malate dehydrogenase thermostable | Sphyraena idiastes | Energy Metabolism | 8 | 14583129 | 1169 | Reduced pH | 32 | 0.14 | 17.88 | 2237.5 | 7.46 | 1826.0 | 8 | 60.86 | 1746.00 | 1740.00 | 2120.16 | 47.78 | 2228.80 | 0.75 | 1.16 | ||||
Hippoglossus hippoglossus (fish) | 3 | Gills 18 °C | 4 | Up | ATP synthase subunit alpha | Salmo salar | Energy Metabolism | 6 | 209151440 | 505 | Reduced pH | 32 | 0.14 | 17.88 | 2237.5 | 7.46 | 1826.0 | 8 | 60.86 | 1746.00 | 1740.00 | 2120.16 | 47.78 | 2228.80 | 0.75 | 1.16 | ||||
Hippoglossus hippoglossus (fish) | 3 | Gills 18 °C | 5 | Up | Putative ribosomal protein S27 | Oncorhynchus mykiss | Protein Biosynthesis/Apoptosis Signalling | 2 | 14787421 | 79 | Reduced pH | 32 | 0.14 | 17.88 | 2237.5 | 7.46 | 1826.0 | 8 | 60.86 | 1746.00 | 1740.00 | 2120.16 | 47.78 | 2228.80 | 0.75 | 1.16 | ||||
Hippoglossus hippoglossus (fish) | 3 | Plasma 12 °C | 1 | Up | Fibrinogen beta chain precursor | Larimichthys crocea | Inflammation Regulator/Immune System Related | 4 | 218665023 | 384 | Reduced pH | 32 | 0.14 | 11.71 | 2229.0 | 7.59 | 1302.5 | 8 | 52.46 | 1247.19 | 1242.55 | 2105.15 | 49.94 | 2207.55 | 0.77 | 1.21 | ||||
Hippoglossus hippoglossus (fish) | 3 | Plasma 12 °C | 1 | Up | Fibrinogen beta chain precursor | Paralichthys olivaceus | Inflammation Regulator/Immune System Related | 3 | 146447341 | 213 | Reduced pH | 32 | 0.14 | 11.71 | 2229.0 | 7.59 | 1302.5 | 8 | 52.46 | 1247.19 | 1242.55 | 2105.15 | 49.94 | 2207.55 | 0.77 | 1.21 | ||||
Hippoglossus hippoglossus (fish) | 3 | Plasma 12 °C | 2 | Down | IgM heavy chain constant region | Hippoglossus hippoglossus | Immune Response Mediator | 8 | 7769631 | 804 | Reduced pH | 32 | 0.14 | 11.71 | 2229.0 | 7.59 | 1302.5 | 8 | 52.46 | 1247.19 | 1242.55 | 2105.15 | 49.94 | 2207.55 | 0.77 | 1.21 | ||||
Hippoglossus hippoglossus (fish) | 3 | Plasma 12 °C | 3 | High-CO2 | Apolipoprotein AI precursor | Platichthys flesus | Immune System Effector/Lipid Carrier | 4 | 60417202 | 686 | Reduced pH | 32 | 0.14 | 11.71 | 2229.0 | 7.59 | 1302.5 | 8 | 52.46 | 1247.19 | 1242.55 | 2105.15 | 49.94 | 2207.55 | 0.77 | 1.21 | ||||
Hippoglossus hippoglossus (fish) | 3 | Plasma 12 °C | 4 | Down | Module-substituted chimera haemoglobin beta-alpha | 6 | 4929993 | 395 | Reduced pH | 32 | 0.14 | 11.71 | 2229.0 | 7.59 | 1302.5 | 8 | 52.46 | 1247.19 | 1242.55 | 2105.15 | 49.94 | 2207.55 | 0.77 | 1.21 | ||||||
Hippoglossus hippoglossus (fish) | 3 | Plasma 12 °C | 5 | High-CO2 | Complement component C3 | Paralichthys olivaceus | Immune System Effector | 8 | 6682835 | 365 | Reduced pH | 32 | 0.14 | 11.71 | 2229.0 | 7.59 | 1302.5 | 8 | 52.46 | 1247.19 | 1242.55 | 2105.15 | 49.94 | 2207.55 | 0.77 | 1.21 | ||||
Hippoglossus hippoglossus (fish) | 3 | Plasma 12 °C | 6 | Up | Complement component C3 | Dicentrarchus labrax | Immune System Effector | 4 | 339269297 | 481 | Reduced pH | 32 | 0.14 | 11.71 | 2229.0 | 7.59 | 1302.5 | 8 | 52.46 | 1247.19 | 1242.55 | 2105.15 | 49.94 | 2207.55 | 0.77 | 1.21 | ||||
Hippoglossus hippoglossus (fish) | 3 | Plasma 18 °C | 1 | Up | Fibrinogen beta chain precursor | Larimichthys crocea | Inflammation Regulator/Immune System Related | 6 | 218665023 | 1228 | Reduced pH | 32 | 0.14 | 17.88 | 2237.5 | 7.46 | 1826.0 | 8 | 60.86 | 1746.00 | 1740.00 | 2120.16 | 47.78 | 2228.80 | 0.75 | 1.16 | ||||
Hippoglossus hippoglossus (fish) | 3 | Plasma 18 °C | 2 | Up | Complement component C3 | Hippoglossus hippoglossus | Immune System Effector | 9 | 58373439 | 627 | Reduced pH | 32 | 0.14 | 17.88 | 2237.5 | 7.46 | 1826.0 | 8 | 60.86 | 1746.00 | 1740.00 | 2120.16 | 47.78 | 2228.80 | 0.75 | 1.16 |