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Kottmeier, Dorothee; Rokitta, Sebastian D; Tortell, Philippe Daniel; Rost, Bjoern (2014): Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects. PANGAEA, https://doi.org/10.1594/PANGAEA.836912, Supplement to: Kottmeier, D et al. (2014): Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects. Photosynthesis Research, 121(2-3), 265-275, https://doi.org/10.1007/s11120-014-9984-9

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Abstract:
Effects of ocean acidification on Emiliania huxleyi strain RCC 1216 (calcifying, diploid life-cycle stage) and RCC 1217 (non-calcifying, haploid life-cycle stage) were investigated by measuring growth, elemental composition, and production rates under different pCO2 levels (380 and 950 µatm). In these differently acclimated cells, the photosynthetic carbon source was assessed by a (14)C disequilibrium assay, conducted over a range of ecologically relevant pH values (7.9-8.7). In agreement with previous studies, we observed decreased calcification and stimulated biomass production in diploid cells under high pCO2, but no CO2-dependent changes in biomass production for haploid cells. In both life-cycle stages, the relative contributions of CO2 and HCO3 (-) uptake depended strongly on the assay pH. At pH values =< 8.1, cells preferentially used CO2 (>= 90 % CO2), whereas at pH values >= 8.3, cells progressively increased the fraction of HCO3 (-) uptake (~45 % CO2 at pH 8.7 in diploid cells; ~55 % CO2 at pH 8.5 in haploid cells). In contrast to the short-term effect of the assay pH, the pCO2 acclimation history had no significant effect on the carbon uptake behavior. A numerical sensitivity study confirmed that the pH-modification in the (14)C disequilibrium method yields reliable results, provided that model parameters (e.g., pH, temperature) are kept within typical measurement uncertainties. Our results demonstrate a high plasticity of E. huxleyi to rapidly adjust carbon acquisition to the external carbon supply and/or pH, and provide an explanation for the paradoxical observation of high CO2 sensitivity despite the apparently high HCO3 (-) usage seen in previous studies.
Keyword(s):
Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; North Atlantic; Other metabolic rates; Pelagos; Phytoplankton; Single species
Further details:
Lavigne, Héloise; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-09-30.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1SpeciesSpeciesKottmeier, Dorothee
2StrainStrainKottmeier, Dorothee
3TreatmentTreatKottmeier, Dorothee
4SalinitySalKottmeier, Dorothee
5Temperature, waterTemp°CKottmeier, Dorothee
6PhosphatePHSPHTµmol/kgKottmeier, Dorotheetotal
7SilicateSILCATµmol/kgKottmeier, Dorotheetotal
8Pressure, waterPressdbarKottmeier, Dorothee
9IrradianceEµmol/m2/sKottmeier, DorotheeFluence rate, 4pi sensor
10pHpHKottmeier, DorotheePotentiometricNBS scale
11Alkalinity, totalATµmol/kgKottmeier, DorotheePotentiometric titration
12Carbon, inorganic, dissolvedDICµmol/kgKottmeier, DorotheeCalculated using CO2SYS
13Growth rateµ1/dayKottmeier, Dorothee
14Carbon, organic, particulate, per cellPOCpg/#Kottmeier, Dorothee
15Total particulate carbon per cellTPC cellpg/#Kottmeier, Dorothee
16Nitrogen, organic, particulate, per cellPONpg/#Kottmeier, Dorothee
17Chlorophyll a per cellChl apg/#Kottmeier, Dorothee
18Light:Dark cycleL:Dhh:hhKottmeier, Dorothee
19Carbon dioxide usage fractionfCO2Kottmeier, Dorotheeat pH 7.9
20Carbon dioxide usage fractionfCO2Kottmeier, Dorotheeat pH 8.1
21Carbon dioxide usage fractionfCO2Kottmeier, Dorotheeat pH 8.3
22Carbon dioxide usage fractionfCO2Kottmeier, Dorotheeat pH 8.5
23Carbon dioxide usage fractionfCO2Kottmeier, Dorotheeat pH 8.7
24Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
25pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
26Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
27Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
28Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
29Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
30Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
31Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
32Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
33Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Size:
548 data points

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