Citation:

s11120-014-9984-9

Name: Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects
Published: 2014-10-21
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; North Atlantic; Other metabolic rates; Pelagos; Phytoplankton; Single species

Always quote citation above when using data! You can download the citation in several formats below.

RIS Citation BibTeX Citation Text Citation Facebook Twitter

Abstract:

Effects of ocean acidification on Emiliania huxleyi strain RCC 1216 (calcifying, diploid life-cycle stage) and RCC 1217 (non-calcifying, haploid life-cycle stage) were investigated by measuring growth, elemental composition, and production rates under different pCO2 levels (380 and 950 µatm). In these differently acclimated cells, the photosynthetic carbon source was assessed by a (14)C disequilibrium assay, conducted over a range of ecologically relevant pH values (7.9-8.7). In agreement with previous studies, we observed decreased calcification and stimulated biomass production in diploid cells under high pCO2, but no CO2-dependent changes in biomass production for haploid cells. In both life-cycle stages, the relative contributions of CO2 and HCO3 (-) uptake depended strongly on the assay pH. At pH values =< 8.1, cells preferentially used CO2 (>= 90 % CO2), whereas at pH values >= 8.3, cells progressively increased the fraction of HCO3 (-) uptake (~45 % CO2 at pH 8.7 in diploid cells; ~55 % CO2 at pH 8.5 in haploid cells). In contrast to the short-term effect of the assay pH, the pCO2 acclimation history had no significant effect on the carbon uptake behavior. A numerical sensitivity study confirmed that the pH-modification in the (14)C disequilibrium method yields reliable results, provided that model parameters (e.g., pH, temperature) are kept within typical measurement uncertainties. Our results demonstrate a high plasticity of E. huxleyi to rapidly adjust carbon acquisition to the external carbon supply and/or pH, and provide an explanation for the paradoxical observation of high CO2 sensitivity despite the apparently high HCO3 (-) usage seen in previous studies.

Keyword(s):

Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; North Atlantic; Other metabolic rates; Pelagos; Phytoplankton; Single species

Further details:

Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-09-30.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Kottmeier, Dorothee
2	Strain	Strain		Kottmeier, Dorothee
3	Treatment	Treat		Kottmeier, Dorothee
4	Salinity	Sal		Kottmeier, Dorothee
5	Temperature, water	Temp	°C	Kottmeier, Dorothee
6	Phosphate	PHSPHT	µmol/kg	Kottmeier, Dorothee		total
7	Silicate	SILCAT	µmol/kg	Kottmeier, Dorothee		total
8	Pressure, water	Press	dbar	Kottmeier, Dorothee
9	Irradiance	E	µmol/m²/s	Kottmeier, Dorothee		Fluence rate, 4pi sensor
10	pH	pH		Kottmeier, Dorothee	Potentiometric	NBS scale
11	Alkalinity, total	AT	µmol/kg	Kottmeier, Dorothee	Potentiometric titration
12	Carbon, inorganic, dissolved	DIC	µmol/kg	Kottmeier, Dorothee	Calculated using CO2SYS
13	Growth rate	µ	1/day	Kottmeier, Dorothee
14	Carbon, organic, particulate, per cell	POC/cell	pg/#	Kottmeier, Dorothee
15	Total particulate carbon per cell	TPC/cell	pg/#	Kottmeier, Dorothee
16	Nitrogen, organic, particulate, per cell	PON/cell	pg/#	Kottmeier, Dorothee
17	Chlorophyll a per cell	Chl a/cell	pg/#	Kottmeier, Dorothee
18	Light:Dark cycle	L:D	hh:hh	Kottmeier, Dorothee
19	Carbon dioxide usage fraction	fCO2		Kottmeier, Dorothee		at pH 7.9
20	Carbon dioxide usage fraction	fCO2		Kottmeier, Dorothee		at pH 8.1
21	Carbon dioxide usage fraction	fCO2		Kottmeier, Dorothee		at pH 8.3
22	Carbon dioxide usage fraction	fCO2		Kottmeier, Dorothee		at pH 8.5
23	Carbon dioxide usage fraction	fCO2		Kottmeier, Dorothee		at pH 8.7
24	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
25	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
26	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
27	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
28	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
29	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
31	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

548 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Species	2 Strain	3 Treat	4 Sal	5 Temp [°C]	6 PHSPHT [µmol/kg] (total)	7 SILCAT [µmol/kg] (total)	8 Press [dbar]	9 E [µmol/m²/s] (Fluence rate, 4pi sensor)	10 pH (NBS scale, Potentiometric)	11 AT [µmol/kg] (Potentiometric titration)	12 DIC [µmol/kg] (Calculated using CO2SYS)	13 µ [1/day]	14 POC/cell [pg/#]	15 TPC/cell [pg/#]	16 PON/cell [pg/#]	17 Chl a/cell [pg/#]	18 L:D [hh:hh]	19 fCO2 (at pH 7.9)	20 fCO2 (at pH 8.1)	21 fCO2 (at pH 8.3)	22 fCO2 (at pH 8.5)	23 fCO2 (at pH 8.7)	24 CSC flag (Calculated using seacarb afte...)	25 pH (total scale, Calculated using...)	26 CO2 [µmol/kg] (Calculated using seacarb afte...)	27 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	28 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	29 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	30 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	31 DIC [µmol/kg] (Calculated using seacarb afte...)	32 Omega Arg (Calculated using seacarb afte...)	33 Omega Cal (Calculated using seacarb afte...)
Emiliania huxleyi (phytoplankton)	RCC 1216	low pCO2 (380 µatm)	32.4	15	6.5	16.7	1	300	8.21	2238	2175	1.09					16:8	1.0000	0.7636	0.6188	0.5087		26	8.10	13.11	346.09	344.86	1835.18	159.96	2008.25	2.48	3.88
Emiliania huxleyi (phytoplankton)	RCC 1216	low pCO2 (380 µatm)	32.4	15	6.5	16.7	1	300	8.20	2239	2175	0.99					16:8	0.9751	0.8086	0.5515	0.6023		26	8.09	13.45	355.12	353.86	1843.00	157.22	2013.67	2.43	3.81
Emiliania huxleyi (phytoplankton)	RCC 1216	low pCO2 (380 µatm)	32.4	15	6.5	16.7	1	300	8.21	2252	2173	1.14					16:8	1.0000	0.7720	0.5775	0.5837		26	8.09	13.44	354.93	353.66	1852.30	158.90	2024.64	2.46	3.85
Emiliania huxleyi (phytoplankton)	RCC 1216	low pCO2 (380 µatm)	32.4	15	6.5	16.7	1	300	8.16	2276	2049	1.04	7.78	20.04	1.52	0.18	16:8			0.6879	0.6582		26	8.04	15.49	408.97	407.51	1908.13	146.34	2069.96	2.27	3.55
Emiliania huxleyi (phytoplankton)	RCC 1216	low pCO2 (380 µatm)	32.4	15	6.5	16.7	1	300	8.18	2275	2042	1.14	7.95	18.83	1.43	0.17	16:8			0.6889			26	8.06	14.60	385.44	384.07	1891.64	152.60	2058.84	2.36	3.70
Emiliania huxleyi (phytoplankton)	RCC 1216	low pCO2 (380 µatm)	32.4	15	6.5	16.7	1	300	8.17	2277	2045	1.06	9.31	21.50	1.67	0.19	16:8						26	8.05	14.92	393.94	392.54	1898.97	150.47	2064.36	2.33	3.65
Emiliania huxleyi (phytoplankton)	RCC 1216	low pCO2 (380 µatm)
Emiliania huxleyi (phytoplankton)	RCC 1216	high pCO2 (950 µatm)	32.4	15	6.5	16.7	1	300	7.89	2259	2088						16:8	1.0000	0.9480	0.7651	0.4681		26	7.78	30.45	803.89	801.02	2041.74	85.24	2157.43	1.32	2.07
Emiliania huxleyi (phytoplankton)	RCC 1216	high pCO2 (950 µatm)	32.4	15	6.5	16.7	1	300	7.90	2268	2079						16:8	0.8505	1.0000	0.6500	0.5101		26	7.78	30.29	799.81	796.96	2048.44	86.24	2164.97	1.34	2.09
Emiliania huxleyi (phytoplankton)	RCC 1216	high pCO2 (950 µatm)	32.4	15	6.5	16.7	1	300	7.90	2260	2073						16:8	1.0000	0.9385	0.6882	0.5709		26	7.78	30.30	799.93	797.08	2041.72	85.66	2157.68	1.33	2.08
Emiliania huxleyi (phytoplankton)	RCC 1216	high pCO2 (950 µatm)	32.4	15	6.5	16.7	1	300	7.79	2309	2156	1.01	14.28	24.51	1.87	0.18	16:8	0.8925					26	7.67	40.74	1075.79	1071.95	2131.62	69.43	2241.79	1.07	1.68
Emiliania huxleyi (phytoplankton)	RCC 1216	high pCO2 (950 µatm)	32.4	15	6.5	16.7	1	300	7.84	2290	2171	1.08	16.89	27.99	2.20	0.18	16:8						26	7.73	34.95	922.82	919.53	2091.40	77.91	2204.26	1.21	1.89
Emiliania huxleyi (phytoplankton)	RCC 1216	high pCO2 (950 µatm)	32.4	15	6.5	16.7	1	300	7.85	2283	2157	1.03	13.16	24.54	1.78	0.16	16:8						26	7.73	34.65	914.96	911.70	2084.00	78.02	2196.68	1.21	1.89
Emiliania huxleyi (phytoplankton)	RCC 1216	high pCO2 (950 µatm)
Emiliania huxleyi (phytoplankton)	RCC 1217	low pCO2 (380 µatm)	32.4	15	6.5	16.7	1	300	8.23	2329	2200	1.12	10.51	10.52	1.33	0.09	16:8	1.0000	1.0000	0.9285	0.4817	0.4472	26	8.11	13.20	348.53	347.29	1902.75	170.75	2086.70	2.64	4.14
Emiliania huxleyi (phytoplankton)	RCC 1217	low pCO2 (380 µatm)	32.4	15	6.5	16.7	1	300	8.23	2306	2196	1.14	10.78	11.35	1.41	0.10	16:8	1.0000	1.0000	1.0000	0.4611	0.3821	26	8.11	12.85	339.21	338.01	1878.39	170.98	2062.21	2.65	4.14
Emiliania huxleyi (phytoplankton)	RCC 1217	low pCO2 (380 µatm)	32.4	15	6.5	16.7	1	300	8.23	2316	2205	1.11	10.98	11.83	1.43	0.11	16:8	1.0000	1.0000	1.0000	0.7154	0.4067	26	8.11	13.16	347.46	346.22	1892.52	169.44	2075.12	2.62	4.11
Emiliania huxleyi (phytoplankton)	RCC 1217	low pCO2 (380 µatm)																	1.0000	1.0000
Emiliania huxleyi (phytoplankton)	RCC 1217	low pCO2 (380 µatm)
Emiliania huxleyi (phytoplankton)	RCC 1217	high pCO2 (950 µatm)	32.4	15	6.5	16.7	1	300	7.90	2319	2031	1.14	10.95	10.62	1.37	0.12	16:8	1.0000	1.0000	0.7970	0.4883	0.3711	26	7.78	30.63	808.66	805.78	2093.37	89.08	2213.07	1.38	2.16
Emiliania huxleyi (phytoplankton)	RCC 1217	high pCO2 (950 µatm)	32.4	15	6.5	16.7	1	300	7.88	2314	2047	1.06	12.32	12.30	1.54	0.11	16:8	1.0000	1.0000	0.8650	0.7043	0.5780	26	7.76	32.55	859.55	856.49	2099.96	84.33	2216.85	1.31	2.04
Emiliania huxleyi (phytoplankton)	RCC 1217	high pCO2 (950 µatm)	32.4	15	6.5	16.7	1	300	7.88	2318	2031	1.03	9.96	13.11	1.43	0.13	16:8	1.0000	0.8698	1.0000	0.7536		26	7.77	31.90	842.31	839.31	2099.81	86.05	2217.76	1.33	2.08