Schmithüsen, Tosia; Niehoff, Barbara; Auel, Holger (2014): Enzyme activities of Calanus finmarchicus from the North Atlantic collected during Maria S. Merian cruise MSM26 in spring 2013 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.835177, In: Schmithüsen, T et al. (2014): Enzyme activities of digestive and metabolic enzymes of Calanus finmarchicus from Maria S. Merian cruise MSM26 in spring 2013 [dataset publication series]. PANGAEA, https://doi.org/10.1594/PANGAEA.835181
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Abstract:
The activities of proteinases, lipases/esterases and citrate synthase of Calanus finmarchicus copepodites (CV) were analysed. Analysis was performed at 30°C for copepods from seven stations (126-9, 127-17, 131-17, 133-6, 134-19, 135-16, 136-8). In addition, thermal profiles (5-50°C) of these enzymes were analysed for copepods from 3 stations (127-17, 133-6, 135-16). C. finmarchicus of station 127-19 have been acclimated on board to two different temperatures (4 and 15°C) for two weeks. Thermal profiles (5-60°C) of lipases/esterases and proteinases of adult females from each treatment were analysed. Groups of 10 individuals were used to prepare enzyme extracts for analysis. From each station/treatment, three groups were analysed, each of which was measured in triplicates.
The activity of proteinases was determined photometrically after Saborowski et al. (2004, hdl:10013/epic.20836), modified after Kreibich et al. (2008, doi:10.1007/s10152-008-0112-0). Azocasein was used as substrate. The lypolytic activity of lipases and esterases in the extract was analysed fluorometrically after Knotz et al. (2006, doi:10.1016/j.cbpa.2006.07.019) using 4-methylumbelliferyl butyrate as substrate. Citrate synthase activity was analysed photometrically after Stitt (1984) modified by Saborowski and Buchholz (2002) with oxaloacetic acid as substrate.
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Related to:
Schmithüsen, Tosia (2013): Enzyme activities in Calanus finmarchicus across the North Atlantic - a comparison of populations from different longitudes [thesis]. Master Thesis, University of Bremen, Alfred Wegener Institut für Polar und Meeresforschung, Bremerhaven, hdl:10013/epic.43161
Project(s):
Basin Scale Analysis, Synthesis and Integration (EURO-BASIN)
Funding:
Seventh Framework Programme (FP7), grant/award no. 264933: Basin Scale Analysis, Synthesis and Integration
Coverage:
Median Latitude: 60.731067 * Median Longitude: -31.636200 * South-bound Latitude: 56.446000 * West-bound Longitude: -56.074000 * North-bound Latitude: 62.858000 * East-bound Longitude: -10.869000
Date/Time Start: 2013-03-25T13:24:00 * Date/Time End: 2013-04-11T10:43:00
Minimum DEPTH, water: 250 m * Maximum DEPTH, water: 700 m
Event(s):
MSM26_126-9 * Latitude Start: 61.498000 * Longitude Start: -10.996000 * Latitude End: 61.445000 * Longitude End: -10.869000 * Date/Time Start: 2013-03-25T13:24:00 * Date/Time End: 2013-03-25T16:27:00 * Elevation Start: -1321.0 m * Elevation End: -1217.0 m * Location: North Atlantic * Campaign: MSM26 * Basis: Maria S. Merian * Method/Device: Double opening/closing plankton net (D-MOC) * Comment: rope length max. 1855m /EL II
MSM26_127-17 * Latitude Start: 62.819000 * Longitude Start: -21.360000 * Latitude End: 62.738000 * Longitude End: -21.294000 * Date/Time Start: 2013-03-28T12:53:00 * Date/Time End: 2013-03-28T15:34:00 * Elevation Start: -1144.0 m * Elevation End: -1175.0 m * Location: North Atlantic * Campaign: MSM26 * Basis: Maria S. Merian * Method/Device: Double opening/closing plankton net (D-MOC) * Comment: rope length max. 2006m /EL II
MSM26_127-19 * Latitude: 62.858000 * Longitude: -21.442000 * Date/Time: 2013-03-28T18:59:00 * Elevation: -1133.0 m * Location: North Atlantic * Campaign: MSM26 * Basis: Maria S. Merian * Method/Device: WP-2 towed closing plankton net (WP2) * Comment: rope length max. 400m /SEW
Parameter(s):
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Size:
3564 data points
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