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Schmithüsen, Tosia; Niehoff, Barbara; Auel, Holger (2014): Enzyme activities of Calanus finmarchicus from the North Atlantic collected during Maria S. Merian cruise MSM26 in spring 2013 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.835177, In: Schmithüsen, T et al. (2014): Enzyme activities of digestive and metabolic enzymes of Calanus finmarchicus from Maria S. Merian cruise MSM26 in spring 2013 [dataset publication series]. PANGAEA, https://doi.org/10.1594/PANGAEA.835181

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Abstract:
The activities of proteinases, lipases/esterases and citrate synthase of Calanus finmarchicus copepodites (CV) were analysed. Analysis was performed at 30°C for copepods from seven stations (126-9, 127-17, 131-17, 133-6, 134-19, 135-16, 136-8). In addition, thermal profiles (5-50°C) of these enzymes were analysed for copepods from 3 stations (127-17, 133-6, 135-16). C. finmarchicus of station 127-19 have been acclimated on board to two different temperatures (4 and 15°C) for two weeks. Thermal profiles (5-60°C) of lipases/esterases and proteinases of adult females from each treatment were analysed. Groups of 10 individuals were used to prepare enzyme extracts for analysis. From each station/treatment, three groups were analysed, each of which was measured in triplicates.
The activity of proteinases was determined photometrically after Saborowski et al. (2004, hdl:10013/epic.20836), modified after Kreibich et al. (2008, doi:10.1007/s10152-008-0112-0). Azocasein was used as substrate. The lypolytic activity of lipases and esterases in the extract was analysed fluorometrically after Knotz et al. (2006, doi:10.1016/j.cbpa.2006.07.019) using 4-methylumbelliferyl butyrate as substrate. Citrate synthase activity was analysed photometrically after Stitt (1984) modified by Saborowski and Buchholz (2002) with oxaloacetic acid as substrate.
For detailed description please contact the author.
Related to:
Schmithüsen, Tosia (2013): Enzyme activities in Calanus finmarchicus across the North Atlantic - a comparison of populations from different longitudes [thesis]. Master Thesis, University of Bremen, Alfred Wegener Institut für Polar und Meeresforschung, Bremerhaven, hdl:10013/epic.43161
Funding:
Seventh Framework Programme (FP7), grant/award no. 264933: Basin Scale Analysis, Synthesis and Integration
Coverage:
Median Latitude: 60.731067 * Median Longitude: -31.636200 * South-bound Latitude: 56.446000 * West-bound Longitude: -56.074000 * North-bound Latitude: 62.858000 * East-bound Longitude: -10.869000
Date/Time Start: 2013-03-25T13:24:00 * Date/Time End: 2013-04-11T10:43:00
Minimum DEPTH, water: 250 m * Maximum DEPTH, water: 700 m
Event(s):
MSM26_126-9  * Latitude Start: 61.498000 * Longitude Start: -10.996000 * Latitude End: 61.445000 * Longitude End: -10.869000 * Date/Time Start: 2013-03-25T13:24:00 * Date/Time End: 2013-03-25T16:27:00 * Elevation Start: -1321.0 m * Elevation End: -1217.0 m * Location: North Atlantic * Campaign: MSM26 * Basis: Maria S. Merian * Method/Device: Double opening/closing plankton net (D-MOC) * Comment: rope length max. 1855m /EL II
MSM26_127-17  * Latitude Start: 62.819000 * Longitude Start: -21.360000 * Latitude End: 62.738000 * Longitude End: -21.294000 * Date/Time Start: 2013-03-28T12:53:00 * Date/Time End: 2013-03-28T15:34:00 * Elevation Start: -1144.0 m * Elevation End: -1175.0 m * Location: North Atlantic * Campaign: MSM26 * Basis: Maria S. Merian * Method/Device: Double opening/closing plankton net (D-MOC) * Comment: rope length max. 2006m /EL II
MSM26_127-19  * Latitude: 62.858000 * Longitude: -21.442000 * Date/Time: 2013-03-28T18:59:00 * Elevation: -1133.0 m * Location: North Atlantic * Campaign: MSM26 * Basis: Maria S. Merian * Method/Device: WP-2 towed closing plankton net (WP2) * Comment: rope length max. 400m /SEW
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
Event labelEvent
Latitude of eventLatitude
Longitude of eventLongitude
Date/Time of eventDate/Time
DEPTH, waterDepth watermGeocode
Depth, top/minDepth topmSchmithüsen, Tosia
Depth, bottom/maxDepth botmSchmithüsen, Tosia
Sample IDSample IDSchmithüsen, Tosia
TreatmentTreatSchmithüsen, Tosia
10 Taxon/taxaTaxaSchmithüsen, Tosia
11 Uniform resource locator/link to referenceURL refSchmithüsen, TosiaWoRMS Aphia ID
12 Life stageLife stageSchmithüsen, Tosia
13 Treatment: temperatureT:temp°CSchmithüsen, Tosiaacclimation temperature
14 Treatment: temperatureT:temp°CSchmithüsen, Tosiaincubation temperature
15 DateDateSchmithüsen, Tosiaof analysis
16 Number of individualsInd No#Schmithüsen, Tosia
17 Absorbance of sampleAbsorb sampSchmithüsen, TosiaSpectrophotometer Thermo Scientific UV1replicate measurement 1, proteinase activity
18 Absorbance of sampleAbsorb sampSchmithüsen, TosiaSpectrophotometer Thermo Scientific UV1replicate measurement 2, proteinase activity
19 Absorbance of sampleAbsorb sampSchmithüsen, TosiaSpectrophotometer Thermo Scientific UV1replicate measurement 3, proteinase activity
20 Absorbance of controlAbsorb contrSchmithüsen, TosiaSpectrophotometer Thermo Scientific UV1replicate measurement 1, proteinase activity
21 Absorbance of controlAbsorb contrSchmithüsen, TosiaSpectrophotometer Thermo Scientific UV1replicate measurement 2, proteinase activity
22 Absorbance of controlAbsorb contrSchmithüsen, TosiaSpectrophotometer Thermo Scientific UV1replicate measurement 3, proteinase activity
23 Proteinase activity per individual, Delta extinction at 366 nmProteinase act/Ind DE3661/h/#Schmithüsen, Tosia
24 Proteinase activity, per dry mass, Delta extinction at 366 nmProteinase act/dm DE3661/h/mgSchmithüsen, Tosia
25 Absorbance of sampleAbsorb sampSchmithüsen, TosiaSpectrophotometer Thermo Scientific UV1replicate measurement 1, lipase activity
26 Absorbance of sampleAbsorb sampSchmithüsen, TosiaSpectrophotometer Thermo Scientific UV1replicate measurement 2, lipase activity
27 Absorbance of sampleAbsorb sampSchmithüsen, TosiaSpectrophotometer Thermo Scientific UV1replicate measurement 3, lipase activity
28 Absorbance of controlAbsorb contrSchmithüsen, TosiaSpectrophotometer Thermo Scientific UV1replicate measurement 1, lipase activity
29 Absorbance of controlAbsorb contrSchmithüsen, TosiaSpectrophotometer Thermo Scientific UV1replicate measurement 2, lipase activity
30 Absorbance of controlAbsorb contrSchmithüsen, TosiaSpectrophotometer Thermo Scientific UV1replicate measurement 3, lipase activity
31 Lipase activity per individualLipase activity/indnmol/min/#Schmithüsen, Tosia
32 Lipase activity, dry massLipase activitynmol/min/mgSchmithüsen, Tosia
33 Absorbance of sampleAbsorb sampSchmithüsen, TosiaSpectrophotometer Thermo Scientific UV1replicate measurement 1, citrate synthase activity
34 Absorbance of sampleAbsorb sampSchmithüsen, TosiaSpectrophotometer Thermo Scientific UV1replicate measurement 2, citrate synthase activity
35 Absorbance of sampleAbsorb sampSchmithüsen, TosiaSpectrophotometer Thermo Scientific UV1replicate measurement 3, citrate synthase activity
36 Absorbance of controlAbsorb contrSchmithüsen, TosiaSpectrophotometer Thermo Scientific UV1replicate measurement 1, citrate synthase activity
37 Absorbance of controlAbsorb contrSchmithüsen, TosiaSpectrophotometer Thermo Scientific UV1replicate measurement 2, citrate synthase activity
38 Absorbance of controlAbsorb contrSchmithüsen, TosiaSpectrophotometer Thermo Scientific UV1replicate measurement 3, citrate synthase activity
39 Citrate synthase activity per individualCitrate synthase activity/indµmol/min/#Schmithüsen, Tosia
40 Citrate synthase activity, dry massCitrate synthase activityµmol/min/mgSchmithüsen, Tosia
Size:
3564 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


Event

Latitude

Longitude

Date/Time

Depth water [m]

Depth top [m]

Depth bot [m]

Sample ID

Treat
10 
Taxa
11 
URL ref
(WoRMS Aphia ID)
12 
Life stage
13 
T:temp [°C]
(acclimation temperature)
14 
T:temp [°C]
(incubation temperature)
15 
Date
(of analysis)
16 
Ind No [#]
17 
Absorb samp
(replicate measurement 1, prot...)
18 
Absorb samp
(replicate measurement 2, prot...)
19 
Absorb samp
(replicate measurement 3, prot...)
20 
Absorb contr
(replicate measurement 1, prot...)
21 
Absorb contr
(replicate measurement 2, prot...)
22 
Absorb contr
(replicate measurement 3, prot...)
23 
Proteinase act/Ind DE366 [1/h/#]
24 
Proteinase act/dm DE366 [1/h/mg]
25 
Absorb samp
(replicate measurement 1, lipa...)
26 
Absorb samp
(replicate measurement 2, lipa...)
27 
Absorb samp
(replicate measurement 3, lipa...)
28 
Absorb contr
(replicate measurement 1, lipa...)
29 
Absorb contr
(replicate measurement 2, lipa...)
30 
Absorb contr
(replicate measurement 3, lipa...)
31 
Lipase activity/ind [nmol/min/#]
32 
Lipase activity [nmol/min/mg]
33 
Absorb samp
(replicate measurement 1, citr...)
34 
Absorb samp
(replicate measurement 2, citr...)
35 
Absorb samp
(replicate measurement 3, citr...)
36 
Absorb contr
(replicate measurement 1, citr...)
37 
Absorb contr
(replicate measurement 2, citr...)
38 
Absorb contr
(replicate measurement 3, citr...)
39 
Citrate synthase activity/ind [µmol/min/#]
40 
Citrate synthase activity [µmol/min/mg]
MSM26_126-9 61.4980-10.99602013-03-25T13:24700600800110C. finmarchicusmarinespecies.orgCV302013-06-0790.76010.91640.76340.61210.66800.64900.18921.081518.41380.11275.5713.6738.0782.80.1390.7960.061820.060340.062950.000180.00095-0.000480.003010.01721
MSM26_126-961.4980-10.99602013-03-25T13:24700600800116C. finmarchicusmarinespecies.orgCV302013-06-07100.76220.87470.70100.61210.66800.64900.13630.781847.11771.21390.7713.6738.0782.80.1801.0250.091370.098820.095000.000180.00095-0.000480.004180.02389
MSM26_126-961.4980-10.99602013-03-25T13:24700600800134C. finmarchicusmarinespecies.orgCV302013-06-07100.72400.73360.64000.61210.66800.64900.05620.321492.21942.51799.6713.6738.0782.80.1941.1080.090390.089880.087700.000180.00095-0.000480.003930.02244
MSM26_127-17 62.8190-21.36002013-03-28T12:53500400600201C. finmarchicusmarinespecies.orgCV302013-06-11100.81910.71320.82170.60180.76960.68740.09840.432295.42135.32127.7896.6957.6944.80.2431.0650.097890.100830.09733-0.00007-0.00022-0.000750.004370.01912
MSM26_127-1762.8190-21.36002013-03-28T12:53500400600202C. finmarchicusmarinespecies.orgCV302013-06-11100.75680.88020.80170.60180.76960.68740.12660.552406.72219.62234.8896.6957.6944.80.2631.1510.096620.092070.10341-0.00007-0.00022-0.000750.004310.01887
MSM26_127-1762.8190-21.36002013-03-28T12:53500400600203C. finmarchicusmarinespecies.orgCV302013-06-11100.83860.79890.79740.60180.76960.68740.12540.552323.42075.82073.8896.6957.6944.80.2381.0410.093320.098460.09580-0.00007-0.00022-0.000750.004240.01858
MSM26_127-1762.8190-21.36002013-03-28T12:53500400600204C. finmarchicusmarinespecies.orgCV502013-06-19100.92280.76020.91340.62510.60500.71660.21660.951740.91794.71802.91058.61034.81059.50.1430.6270.004100.003900.002300.001600.001800.001200.000080.00037
MSM26_127-1762.8190-21.36002013-03-28T12:53500400600205C. finmarchicusmarinespecies.orgCV502013-06-19100.90700.87930.77900.62510.60500.71660.20620.902026.12114.02046.01058.61034.81059.50.1990.8700.002300.002700.002400.001600.001800.001200.000040.00018
MSM26_127-1762.8190-21.36002013-03-28T12:53500400600206C. finmarchicusmarinespecies.orgCV502013-06-19100.94810.80131.02910.62510.60500.71660.27731.211739.41746.71801.91058.61034.81059.50.1400.6130.002200.010800.001900.001600.001800.001200.000150.00066
MSM26_127-1762.8190-21.36002013-03-28T12:53500400600210C. finmarchicusmarinespecies.orgCV202013-06-24100.75620.74500.78290.66010.62770.65950.11230.491588.41649.51571.5460.8484.2552.20.2311.0100.070400.065300.073900.000400.000200.000100.003070.01345
MSM26_127-1762.8190-21.36002013-03-28T12:53500400600211C. finmarchicusmarinespecies.orgCV202013-06-24100.75740.63690.64430.66010.62770.65950.03040.131591.81546.31594.7460.8484.2552.20.2250.9870.065600.062700.061900.000400.000200.000100.002790.01220
MSM26_127-1762.8190-21.36002013-03-28T12:53500400600212C. finmarchicusmarinespecies.orgCV202013-06-24100.63800.69500.64530.66010.62770.65950.01030.051517.61525.41586.2460.8484.2552.20.2180.9550.059900.060100.066400.000400.000200.000100.002730.01195
MSM26_127-1762.8190-21.36002013-03-28T12:53500400600213C. finmarchicusmarinespecies.orgCV152013-06-25100.72730.67830.78440.68710.60780.61110.09470.41964.6924.8815.3287.6212.3224.10.1240.5450.048700.046700.047700.000100.000000.000000.002100.00920
MSM26_127-1762.8190-21.36002013-03-28T12:53500400600214C. finmarchicusmarinespecies.orgCV152013-06-25100.72690.68260.74340.68710.60780.61110.08230.361074.51078.2947.4287.6212.3224.10.1490.6530.057700.053900.043700.000100.000000.000000.002280.00999
MSM26_127-1762.8190-21.36002013-03-28T12:53500400600215C. finmarchicusmarinespecies.orgCV152013-06-25100.68340.78680.65110.68710.60780.61110.07180.311079.5977.21046.7287.6212.3224.10.1500.6540.046300.050500.045400.000100.000000.000000.002090.00915
MSM26_127-1762.8190-21.36002013-03-28T12:53500400600216C. finmarchicusmarinespecies.orgCV102013-06-26100.78030.73310.64280.69310.62780.69780.04580.201001.1836.2860.8187.1143.4184.20.1400.6130.037500.039000.034300.000000.000500.000100.001620.00790
MSM26_127-1762.8190-21.36002013-03-28T12:53500400600217C. finmarchicusmarinespecies.orgCV102013-06-26100.69820.76860.71430.69310.62780.69780.05410.241274.41199.11243.3187.1143.4184.20.2050.8990.041300.038800.038100.000000.000500.000100.001730.00757
MSM26_127-1762.8190-21.36002013-03-28T12:53500400600218C. finmarchicusmarinespecies.orgCV102013-06-26100.71580.78560.68050.69310.62780.69780.05440.241202.91293.81238.6187.1143.4184.20.2070.9040.039200.041600.039800.000000.000500.000100.001760.00772
MSM26_127-1762.8190-21.36002013-03-28T12:53500400600219C. finmarchicusmarinespecies.orgCV52013-07-02100.62230.68240.66980.62970.65970.61320.02400.10823.8854.8714.6119.6135.3170.80.1400.6110.025300.026400.025400.000000.000000.000000.001130.00496
MSM26_127-1762.8190-21.36002013-03-28T12:53500400600220C. finmarchicusmarinespecies.orgCV52013-07-02100.68400.64960.72180.62970.65970.61320.05090.22764.0763.7759.5119.6135.3170.80.1320.5790.026900.027100.024800.000000.000000.000000.001160.00570
MSM26_127-1762.8190-21.36002013-03-28T12:53500400600221C. finmarchicusmarinespecies.orgCV52013-07-02100.64230.62910.67580.62970.65970.61320.01490.07673.6657.7612.1119.6135.3170.80.1080.4720.022000.025200.021400.000000.000000.000000.001010.00442
MSM26_127-1762.8190-21.36002013-03-28T12:53500400600222C. finmarchicusmarinespecies.orgCV402013-07-10100.72200.87500.73900.65500.67300.66400.11280.492095.62153.72254.7889.3987.81087.00.1970.8630.004400.010800.005500.002500.001800.001000.000230.00099
MSM26_127-1762.8190-21.36002013-03-28T12:53500400600223C. finmarchicusmarinespecies.orgCV402013-07-10100.79200.76300.85800.65500.67300.66400.13800.602889.93006.73065.8889.3987.81087.00.3341.4630.006800.009100.006900.002500.001800.001000.000260.00113
MSM26_127-1762.8190-21.36002013-03-28T12:53500400600224C. finmarchicusmarinespecies.orgCV402013-07-10100.72100.84200.84600.65500.67300.66400.13670.602829.22764.82588.1889.3987.81087.00.2911.2730.007300.008000.008700.002500.001800.001000.000280.00120
MSM26_127-19 62.8580-21.44202013-03-28T18:59400A01acclimation experimentC. finmarchicusmarinespecies.orgCVI, female452013-07-11100.88700.91300.87200.61500.68700.70100.22302192.92110.82025.1157.1167.0165.40.415
MSM26_127-1962.8580-21.44202013-03-28T18:59400A02acclimation experimentC. finmarchicusmarinespecies.orgCVI, female452013-07-11100.76200.89400.79100.61500.68700.70100.14802565.22411.02331.3157.1167.0165.40.484
MSM26_127-1962.8580-21.44202013-03-28T18:59400A03acclimation experimentC. finmarchicusmarinespecies.orgCVI, female452013-07-11100.72700.77700.76300.61500.68700.70100.08802044.82092.22011.3157.1167.0165.40.402
MSM26_127-1962.8580-21.44202013-03-28T18:59400A04acclimation experimentC. finmarchicusmarinespecies.orgCVI, female4102013-07-11100.88800.98201.08000.68800.83700.70600.23972841.23115.72894.4176.9185.8170.60.533
MSM26_127-1962.8580-21.44202013-03-28T18:59400A05acclimation experimentC. finmarchicusmarinespecies.orgCVI, female4102013-07-11101.08000.96900.96600.68800.83700.70600.26132263.71854.91692.8176.9185.8170.60.338
MSM26_127-1962.8580-21.44202013-03-28T18:59400A06acclimation experimentC. finmarchicusmarinespecies.orgCVI, female4102013-07-11100.92901.05800.85500.68800.83700.70600.20372916.93015.33406.0176.9185.8170.60.565
MSM26_127-1962.8580-21.44202013-03-28T18:59400A07acclimation experimentC. finmarchicusmarinespecies.orgCVI, female4152013-07-12101.01201.01100.99000.72900.71400.69700.29104042.43859.63637.9113.278.776.40.708
MSM26_127-1962.8580-21.44202013-03-28T18:59400A08acclimation experimentC. finmarchicusmarinespecies.orgCVI, female4152013-07-12101.05600.90400.97900.72900.71400.69700.26633829.43314.93505.9113.278.776.40.652
MSM26_127-1962.8580-21.44202013-03-28T18:59400A09acclimation experimentC. finmarchicusmarinespecies.orgCVI, female4152013-07-12100.91501.18600.86100.72900.71400.69700.27403533.53755.33760.5113.278.776.40.677
MSM26_127-1962.8580-21.44202013-03-28T18:59400A10acclimation experimentC. finmarchicusmarinespecies.orgCVI, female4202013-07-12101.15401.04601.11000.70600.75300.75300.36604541.14512.84514.2246.1205.1188.50.901
MSM26_127-1962.8580-21.44202013-03-28T18:59400A11acclimation experimentC. finmarchicusmarinespecies.orgCVI, female4202013-07-12101.19801.29701.27500.70600.75300.75300.51934786.54848.94823.5246.1205.1188.50.963
MSM26_127-1962.8580-21.44202013-03-28T18:59400A12acclimation experimentC. finmarchicusmarinespecies.orgCVI, female4202013-07-12100.98801.16800.97400.70600.75300.75300.30603476.43517.03494.6246.1205.1188.50.686
MSM26_127-1962.8580-21.44202013-03-28T18:59400A13acclimation experimentC. finmarchicusmarinespecies.orgCVI, female4302013-07-15100.89361.22721.06520.69270.67350.62500.39835177.95146.24891.3366.2389.3377.00.912
MSM26_127-1962.8580-21.44202013-03-28T18:59400A14acclimation experimentC. finmarchicusmarinespecies.orgCVI, female4302013-07-15101.24891.22631.20130.69270.67350.62500.56188963.97388.18643.6366.2389.3377.01.545
MSM26_127-1962.8580-21.44202013-03-28T18:59400A15acclimation experimentC. finmarchicusmarinespecies.orgCVI, female4302013-07-15101.21521.25261.10300.69270.67350.62500.52656482.26798.96278.0366.2389.3377.01.193
MSM26_127-1962.8580-21.44202013-03-28T18:59400A16acclimation experimentC. finmarchicusmarinespecies.orgCVI, female4402013-07-15101.64491.64221.62820.87930.63810.69370.90145982.05524.06351.0449.5511.6530.31.125
MSM26_127-1962.8580-21.44202013-03-28T18:59400A17acclimation experimentC. finmarchicusmarinespecies.orgCVI, female4402013-07-15101.58851.69861.56990.87930.63810.69370.88205564.05641.15287.5449.5511.6530.31.031
MSM26_127-1962.8580-21.44202013-03-28T18:59400A18acclimation experimentC. finmarchicusmarinespecies.orgCVI, female4402013-07-15101.40761.44131.41060.87930.63810.69370.68285624.95650.25108.8449.5511.6530.31.024
MSM26_127-1962.8580-21.44202013-03-28T18:59400A19acclimation experimentC. finmarchicusmarinespecies.orgCVI, female4502013-07-16101.57941.55561.69140.68660.69030.64470.91966771.16440.46128.6744.9752.3716.31.123
MSM26_127-1962.8580-21.44202013-03-28T18:59400A20acclimation experimentC. finmarchicusmarinespecies.orgCVI, female4502013-07-16101.72821.65221.64340.68660.69030.64470.98435840.35759.45928.5744.9752.3716.31.004
MSM26_127-1962.8580-21.44202013-03-28T18:59400A21acclimation experimentC. finmarchicusmarinespecies.orgCVI, female4502013-07-16101.54691.57001.60220.68660.69030.64470.88445843.95336.05772.3744.9752.3716.30.966
MSM26_127-1962.8580-21.44202013-03-28T18:59400A22acclimation experimentC. finmarchicusmarinespecies.orgCVI, female4602013-07-16101.10291.19991.09180.71140.71910.69830.41503496.43797.03145.71007.0966.71009.60.497
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