Not logged in
PANGAEA.
Data Publisher for Earth & Environmental Science

Eichner, Meri; Kranz, Sven A; Rost, Bjoern (2014): Combined effects of different CO2 levels and N sources on the diazotrophic cyanobacterium Trichodesmium. PANGAEA, https://doi.org/10.1594/PANGAEA.834556, Supplement to: Eichner, M et al. (2014): Combined effects of different CO2 levels and N sources on the diazotrophic cyanobacterium Trichodesmium. Physiologia Plantarum, 152(2), 316-330, https://doi.org/10.1111/ppl.12172

Always quote above citation when using data! You can download the citation in several formats below.

RIS CitationBibTeX Citation

Abstract:
To predict effects of climate change and possible feedbacks, it is crucial to understand the mechanisms behind CO2 responses of biogeochemically relevant phytoplankton species. Previous experiments on the abundant N2 fixers Trichodesmium demonstrated strong CO2 responses, which were attributed to an energy reallocation between its carbon (C) and nitrogen (N) acquisition. Pursuing this hypothesis, we manipulated the cellular energy budget by growing Trichodesmium erythraeum IMS101 under different CO2 partial pressure (pCO2) levels (180, 380, 980 and 1400?µatm) and N sources (N2 and NO3-). Subsequently, biomass production and the main energy-generating processes (photosynthesis and respiration) and energy-consuming processes (N2 fixation and C acquisition) were measured. While oxygen fluxes and chlorophyll fluorescence indicated that energy generation and its diurnal cycle was neither affected by pCO2 nor N source, cells differed in production rates and composition. Elevated pCO2 increased N2 fixation and organic C and N contents. The degree of stimulation was higher for nitrogenase activity than for cell contents, indicating a pCO2 effect on the transfer efficiency from N2 to biomass. pCO2-dependent changes in the diurnal cycle of N2 fixation correlated well with C affinities, confirming the interactions between N and C acquisition. Regarding effects of the N source, production rates were enhanced in NO3-grown cells, which we attribute to the higher N retention and lower ATP demand compared with N2 fixation. pCO2 effects on C affinity were less pronounced in NO3- users than N2 fixers. Our study illustrates the necessity to understand energy budgets and fluxes under different environmental conditions for explaining indirect effects of rising pCO2.
Keyword(s):
Bacteria; Bottles or small containers/Aquaria (<20 L); Cyanobacteria; Growth/Morphology; Laboratory experiment; Laboratory strains; Macro-nutrients; Not applicable; Other metabolic rates; Pelagos; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species; Trichodesmium erythraeum
Further details:
Lavigne, Héloise; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-07-30.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1SpeciesSpeciesEichner, Meri
2FigureFigEichner, Meri
3TreatmentTreatEichner, Meri
4SalinitySalEichner, Meri
5Temperature, waterTemp°CEichner, Meri
6IrradianceEµmol/m2/sEichner, Meri
7pHpHEichner, MeriPotentiometricNBS scale
8pH, standard deviationpH std dev±Eichner, MeriPotentiometricNBS scale
9Alkalinity, totalATµmol/kgEichner, MeriPotentiometric titration
10Alkalinity, total, standard deviationAT std dev±Eichner, MeriPotentiometric titration
11Carbon, inorganic, dissolvedDICµmol/kgEichner, MeriCoulometric titration
12Carbon, inorganic, dissolved, standard deviationDIC std dev±Eichner, MeriCoulometric titration
13Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetppmvEichner, MeriCalculated using CO2SYS
14Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Eichner, MeriCalculated using CO2SYS
15Growth rateµ1/dayEichner, Meri
16Growth rate, standard deviationµ std dev±Eichner, Meri
17Carbon, organic, particulate per chlorophyll aPOC/Chl aµmol/mgEichner, Meri
18Carbon, organic, particulate, standard deviationPOC std dev±Eichner, Meriper Chla
19Nitrogen, organic, particulate per chlorophyll aPON/Chl aµmol/mgEichner, Meri
20Nitrogen, organic, particulate, standard deviationPON std dev±Eichner, Meriper Chla
21Particulate organic carbon production, per chlorophyll aPOC prod/Chl aµmol/µg/dayEichner, Meri
22Particulate organic carbon, production, standard deviationPOC prod std dev±Eichner, Meriper Chl a
23Particulate organic nitrogen production, per chlorophyll aPON prod/Chl aµmol/µg/dayEichner, Meri
24Particulate organic nitrogen production, standard deviationPON prod std dev±Eichner, Meriper Chl a
25Carbon, organic, particulate/Nitrogen, organic, particulate ratioPOC/PONEichner, Meri
26Carbon, organic, particulate/Nitrogen, organic, particulate ratio, standard deviationPOC/PON std dev±Eichner, Meri
27IdentificationIDEichner, Meri
28Time in hoursTimehEichner, Meriafter beginning of photoperiod
29Light:Dark cycleL:Dhh:hhEichner, Meri
30Nitrogen fixation rate per chlorophyll aN2 fix/Chlnmol/µg/hEichner, Meri
31Time point, descriptiveTime pointEichner, Meri
32Net oxygen evolution, per chlorophyll aO2 ev/Chlµmol/mg/hEichner, Merimaximum rate
33Net oxygen evolution, per chlorophyll a, standard deviationO2 ev/Chl std dev±Eichner, Merimaximum rate
34Carbon, inorganic, dissolved, half saturation concentrationK1/2 DICµmol/lEichner, Meri
35Carbon, inorganic, dissolved, half saturation concentration, standard deviationK1/2 DIC std dev±Eichner, Meri
36Maximum photochemical quantum yield of photosystem IIFv/FmEichner, Merimeasured in dark
37Maximum photochemical quantum yield of photosystem II, standard deviationFv/Fm std dev±Eichner, Merimeasured in dark
38Maximum photochemical quantum yield of photosystem IIFv/FmEichner, Merimeasured in light
39Maximum photochemical quantum yield of photosystem II, standard deviationFv/Fm std dev±Eichner, Merimeasured in light
40Effective absorbance cross-section of photosystem IIsigma PSIIA2/quantaEichner, Merimeasured in dark
41Effective absorbance cross-section of photosystem II, standard deviationsigma PSII std dev±Eichner, Merimeasured in dark
42Effective absorbance cross-section of photosystem IIsigma PSIIA2/quantaEichner, Merimeasured in light
43Effective absorbance cross-section of photosystem II, standard deviationsigma PSII std dev±Eichner, Merimeasured in light
44Re-oxidation time of the Qa acceptortQaµsEichner, Merimeasured in dark
45Re-oxidation time of the Qa acceptor, standard deviationtQa std dev±Eichner, Merimeasured in dark
46Re-oxidation time of the Qa acceptortQaµsEichner, Merimeasured in light
47Re-oxidation time of the Qa acceptor, standard deviationtQa std dev±Eichner, Merimeasured in light
48Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
49pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
50Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
51Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
52Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
53Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
54Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
55Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
56Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
57Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Size:
1523 data points

Download Data

Download dataset as tab-delimited text (use the following character encoding: )

View dataset as HTML