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Eichner, Meri; Rost, Björn; Kranz, Sven A (2014): Diversity of ocean acidification effects on marine N2 fixers. PANGAEA, https://doi.org/10.1594/PANGAEA.834555, Supplement to: Eichner, M et al. (2014): Diversity of ocean acidification effects on marine N2 fixers. Journal of Experimental Marine Biology and Ecology, 457, 199-207, https://doi.org/10.1016/j.jembe.2014.04.015

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Abstract:
Considering the important role of N2 fixation for primary productivity and CO2 sequestration, it is crucial to assess the response of diazotrophs to ocean acidification. Previous studies on the genus Trichodesmium suggested a strong sensitivity towards ocean acidification. In view of the large functional diversity in N2 fixers, the objective of this study was to improve our knowledge of the CO2 responses of other diazotrophs. To this end, the single-celled Cyanothece sp. and two heterocystous species, Nodularia spumigena and the symbiotic Calothrix rhizosoleniae, were acclimated to two pCO2 levels (380 vs. 980 µatm). Growth rates, cellular composition (carbon, nitrogen and chlorophyll a) as well as carbon and N2 fixation rates (14C incorporation, acetylene reduction) were measured and compared to literature data on different N2 fixers. The three species investigated in this study responded differently to elevated pCO2, showing enhanced, decreased as well as unaltered growth and production rates. For instance, Cyanothece increased production rates with pCO2, which is in line with the general view that N2 fixers benefit from ocean acidification. Due to lowered growth and production of Nodularia, nitrogen input to the Baltic Sea might decrease in the future. In Calothrix, no significant changes in growth or production could be observed, even though N2 fixation was stimulated under elevated pCO2. Reviewing literature data confirmed a large variability in CO2 sensitivity across diazotrophs. The contrasting response patterns in our and previous studies were discussed with regard to the carbonate chemistry in the respective natural habitats, the mode of N2 fixation as well as differences in cellular energy limitation between the species. The group-specific CO2 sensitivities will impact differently on future biogeochemical cycles of open-ocean environments and systems like the Baltic Sea and should therefore be considered in models estimating climate feedback effects.
Keyword(s):
Bacteria; Bottles or small containers/Aquaria (<20 L); Calothrix rhizosoleniae; Cyanobacteria; Cyanothece sp.; Growth/Morphology; Laboratory experiment; Laboratory strains; Nodularia spumigena; Not applicable; Other metabolic rates; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species
Further details:
Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-07-30.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
SpeciesSpeciesEichner, Meri
TreatmentTreatEichner, Meri
SalinitySalEichner, Meri
Temperature, waterTemp°CEichner, Meri
IrradianceEµmol/m2/sEichner, Meri
pHpHEichner, MeriPotentiometricNBS scale
pH, standard deviationpH std dev±Eichner, MeriPotentiometricNBS scale
Alkalinity, totalATµmol/kgEichner, MeriPotentiometric titration
Alkalinity, total, standard deviationAT std dev±Eichner, MeriPotentiometric titration
10 Carbon, inorganic, dissolvedDICµmol/kgEichner, MeriCoulometric titration
11 Carbon, inorganic, dissolved, standard deviationDIC std dev±Eichner, MeriCoulometric titration
12 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmEichner, MeriCalculated using CO2SYS
13 Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Eichner, MeriCalculated using CO2SYS
14 Growth rateµ1/dayEichner, Meri
15 Growth rate, standard deviationµ std dev±Eichner, Meri
16 Particulate organic carbon production, per chlorophyll aPOC prod/Chl aµmol/µg/dayEichner, Meri
17 Particulate organic carbon, production, standard deviationPOC prod std dev±Eichner, Meriper Chl a
18 Particulate organic nitrogen production, per chlorophyll aPON prod/Chl aµmol/µg/dayEichner, Meri
19 Particulate organic nitrogen production, standard deviationPON prod std dev±Eichner, Meriper Chl a
20 Carbon fixation rate, per chlorophyll aC fix/Chl aµmol/µg/dayEichner, Meri
21 Carbon fixation rate, per chlorophyll a, standard deviationC fix/Chl a std dev±Eichner, Meri
22 Ethene production, per chlorophyll aC2H4 prod/Chl aµmol/µg/dayEichner, Meri
23 Ethene production standard deviationC2H4 prod std dev±Eichner, Meriper Chl a
24 Carbon, organic, particulate/Nitrogen, organic, particulate ratioPOC/PONEichner, Meri
25 Carbon, organic, particulate/Nitrogen, organic, particulate ratio, standard deviationPOC/PON std dev±Eichner, Meri
26 Ethene production per cellC2H4 prod/cellpmol/#/dayEichner, Meri
27 Ethene production standard deviationC2H4 prod std dev±Eichner, Meriper cell
28 Chlorophyll a per cellChl a/cellpg/#Eichner, Meri
29 Chlorophyll a, standard deviationChl a std dev±Eichner, Meri
30 Particulate organic carbon, per cellPOC/cellpmol/#Eichner, Meri
31 Particulate organic carbon content per cell, standard deviationPOC cont/cell std dev±Eichner, Meri
32 Particulate organic nitrogen per cellPON/cellpmol/#Eichner, Meri
33 Particulate organic nitrogen per cell, standard deviationPON/cell std dev±Eichner, Meri
34 Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
35 pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
36 Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
37 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
38 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
39 Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
40 Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
41 Alkalinity, totalATµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
42 Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
43 Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
241 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


Species

Treat

Sal

Temp [°C]

E [µmol/m2/s]

pH
(NBS scale, Potentiometric)

pH std dev [±]
(NBS scale, Potentiometric)

AT [µmol/kg]
(Potentiometric titration)

AT std dev [±]
(Potentiometric titration)
10 
DIC [µmol/kg]
(Coulometric titration)
11 
DIC std dev [±]
(Coulometric titration)
12 
pCO2water_SST_wet [µatm]
(Calculated using CO2SYS)
13 
pCO2 std dev [±]
(Calculated using CO2SYS)
14 
µ [1/day]
15 
µ std dev [±]
16 
POC prod/Chl a [µmol/µg/day]
17 
POC prod std dev [±]
(per Chl a)
18 
PON prod/Chl a [µmol/µg/day]
19 
PON prod std dev [±]
(per Chl a)
20 
C fix/Chl a [µmol/µg/day]
21 
C fix/Chl a std dev [±]
22 
C2H4 prod/Chl a [µmol/µg/day]
23 
C2H4 prod std dev [±]
(per Chl a)
24 
POC/PON
25 
POC/PON std dev [±]
26 
C2H4 prod/cell [pmol/#/day]
27 
C2H4 prod std dev [±]
(per cell)
28 
Chl a/cell [pg/#]
29 
Chl a std dev [±]
30 
POC/cell [pmol/#]
31 
POC cont/cell std dev [±]
32 
PON/cell [pmol/#]
33 
PON/cell std dev [±]
34 
CSC flag
(Calculated using seacarb afte...)
35 
pH
(total scale, Calculated using...)
36 
CO2 [µmol/kg]
(Calculated using seacarb afte...)
37 
pCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)
38 
fCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)
39 
[HCO3]- [µmol/kg]
(Calculated using seacarb afte...)
40 
[CO3]2- [µmol/kg]
(Calculated using seacarb afte...)
41 
AT [µmol/kg]
(Calculated using seacarb afte...)
42 
Omega Arg
(Calculated using seacarb afte...)
43 
Omega Cal
(Calculated using seacarb afte...)
Cyanothece sp. ATCC 51142 (prokaryote)ambient pCO233251508.200.01235621969138090.390.046.61.51.380.280.650.353.251.414.750.230.110.040.040.000.600.060.130.01278.0610.86379.91378.721750.01208.132259.503.345.09
Cyanothece sp. ATCC 51142 (prokaryote)high pCO233251507.860.02236813215410974200.370.0216.41.53.390.401.510.833.850.964.850.130.120.010.030.001.270.170.260.04277.7227.40958.02955.002016.96109.642284.221.762.68
Nodularia spumigena IOW 2000/1(prokaryote)ambient pCO29251508.180.0215242813571239170.330.062.00.60.410.140.870.610.094.920.140.400.080.650.124.081.500.830.29278.0212.61389.20387.971277.2567.141428.571.172.02
Nodularia spumigena IOW 2000/1(prokaryote)high pCO29251507.800.02151517139925974390.140.061.50.20.290.050.540.280.570.205.160.040.300.110.530.054.170.360.810.07277.6431.64976.51973.441338.0029.361404.300.510.88
Calothrix rhizosoleniae SC01 (prokaryote)ambient pCO233251508.250.02234816195013340300.250.081.80.60.280.100.840.700.450.076.470.13278.119.47331.26330.221712.07228.462271.053.675.59
Calothrix rhizosoleniae SC01 (prokaryote)high pCO233251507.930.03233928210218832700.360.032.40.20.340.060.390.100.730.036.640.15277.7922.60790.20787.711954.57124.832259.572.003.05