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Fernández-Méndez, Mar; Peeken, Ilka; Boetius, Antje (2014): Carbon uptake rate calculated for CTD water samples during POLARSTERN cruise ARK-XXVII/3 (IceArc) in 2012. PANGAEA, https://doi.org/10.1594/PANGAEA.834217, In supplement to: Fernández-Méndez, Mar; Katlein, Christian; Rabe, Benjamin; Nicolaus, Marcel; Peeken, Ilka; Bakker, Karel; Flores, Hauke; Boetius, Antje (2015): Photosynthetic production in the central Arctic Ocean during the record sea-ice minimum in 2012. Biogeosciences, 12, 3525-3549, https://doi.org/10.5194/bg-12-3525-2015

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Abstract:
Net Primary Production was measured using the 14**C uptake method with minor modifications. Seawater samples were spiked with 0.1µCi ml**-1 of 14**C labelled sodium bicarbonate (Moravek Biochemicals, Brea, USA) and distributed in 10 clear bottles (20 ml each). Subsequently they were incubated for 12 h at -1.3°C under different scalar irradiances (0-420 µmol photons m**-2 s**-1) measured with a spherical sensor (Spherical Micro Quantum Sensor US-SQS/L, Heinz Walz, Effeltrich, Germany). At the end of the incubation, samples were filtered onto 0.2 µm nitrocellulose filters and the particulate radioactive carbon uptake was determined by liquid scintillation counting using Filter count scintillation cocktail (Perkin Elmer, Waltham, USA). The carbon uptake values in the dark were subtracted from the carbon uptake values measured in the light incubations. Dissolved inorganic carbon (DIC) was measured for each sample using the flow injection system (Hall and Aller, 1992). The DIC concentration was taken into account to calculate the amount of labeled bicarbonate incorporated into the cell. Carbon fixation rates were normalized volumetrically and by chlorophyll a. Photosynthesis-irradiance curves (PI curves) were fitted using MATLAB® according to the equation proposed by Platt et al. (1980) including a photoinhibition parameter (beta) and providing the main photosynthetic parameters: maximum Chla normalized carbon fixation rate if there were no photoinhibition (Pb) and the initial slope of the saturation curve (alpha). The derived parameters: light intensity at which photosynthesis is maximal (Im), the carbon fixation rate at that maximal irradiance (Pbm) and the adaptation parameter or photoacclimation index (Ik) were calculated according to Platt et al. (1982).
Source data set:
Bakker, Karel (2014): Nutrients measured on water bottle samples during POLARSTERN cruise ARK-XXVII/3 (IceArc) in 2012. PANGAEA, https://doi.org/10.1594/PANGAEA.834081
Funding:
Seventh Framework Programme (FP7), grant/award no. 294757: Assessment of bacterial life and matter cycling in deep-sea surface sediments
Coverage:
Median Latitude: 83.456230 * Median Longitude: 85.220811 * South-bound Latitude: 77.396830 * West-bound Longitude: 17.453670 * North-bound Latitude: 88.827670 * East-bound Longitude: 133.341830
Date/Time Start: 2012-08-06T07:04:00 * Date/Time End: 2012-09-29T15:10:00
Minimum DEPTH, water: m * Maximum DEPTH, water: 50 m
Event(s):
PS80/209-1 * Latitude: 81.493500 * Longitude: 30.172170 * Date/Time: 2012-08-06T07:04:00 * Elevation: -709.1 m * Location: Arctic Ocean * Campaign: ARK-XXVII/3 (PS80 IceArc) * Basis: Polarstern * Method/Device: CTD/Rosette (CTD-RO) * Comment: 32.1 708 m; Position sensor: Ship's GPS
PS80/213-1 * Latitude: 81.835830 * Longitude: 29.953830 * Date/Time: 2012-08-06T20:10:00 * Elevation: -3151.7 m * Location: Arctic Ocean * Campaign: ARK-XXVII/3 (PS80 IceArc) * Basis: Polarstern * Method/Device: CTD/Rosette (CTD-RO) * Comment: 3202 32.1; Position sensor: Ship's GPS
PS80/215-1 * Latitude: 82.495170 * Longitude: 30.003000 * Date/Time: 2012-08-07T05:32:00 * Elevation: -3626.8 m * Location: Arctic Ocean * Campaign: ARK-XXVII/3 (PS80 IceArc) * Basis: Polarstern * Method/Device: CTD/Rosette (CTD-RO) * Comment: 3694 m, SE 32.1; Position sensor: Ship's GPS
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Sample code/labelSample label
2Optional event labelEvent 2
3GearGear
4DescriptionDescription
5Latitude of eventLatitude
6Longitude of eventLongitude
7Date/Time of eventDate/Time
8Date/time startDate/time start
9Date/time endDate/time end
10CommentComment
11DEPTH, waterDepth watermGeocode
12Carbon uptake rate, fractionatedC upt rate fracµg/l/dayBoetius, AntjeMeasuredat light irradiance 420 [µmol photons/m**2/s]
13Carbon uptake rate, fractionatedC upt rate fracµg/l/dayBoetius, AntjeMeasuredat light irradiance 370 [µmol photons/m**2/s]
14Carbon uptake rate, fractionatedC upt rate fracµg/l/dayBoetius, AntjeMeasuredat light irradiance 150 [µmol photons/m**2/s]
15Carbon uptake rate, fractionatedC upt rate fracµg/l/dayBoetius, AntjeMeasuredat light irradiance 90 [µmol photons/m**2/s]
16Carbon uptake rate, fractionatedC upt rate fracµg/l/dayBoetius, AntjeMeasuredat light irradiance 65 [µmol photons/m**2/s]
17Carbon uptake rate, fractionatedC upt rate fracµg/l/dayBoetius, AntjeMeasuredat light irradiance 50 [µmol photons/m**2/s]
18Carbon uptake rate, fractionatedC upt rate fracµg/l/dayBoetius, AntjeMeasuredat light irradiance 25 [µmol photons/m**2/s]
19Carbon uptake rate, fractionatedC upt rate fracµg/l/dayBoetius, AntjeMeasuredat light irradiance 13 [µmol photons/m**2/s]
20Carbon uptake rate, fractionatedC upt rate fracµg/l/dayBoetius, AntjeMeasuredat light irradiance 8 [µmol photons/m**2/s]
21Carbon uptake rate, fractionatedC upt rate fracµg/l/dayBoetius, AntjeMeasuredat light irradiance 0 [µmol photons/m**2/s]
22Carbon uptake rate, fractionated, normalized to chlorophyll aC upt rate frac norm chl amg/mg/hBoetius, AntjeMeasuredat light irradiance 420 [µmol photons/m**2/s]
23Carbon uptake rate, fractionated, normalized to chlorophyll aC upt rate frac norm chl amg/mg/hBoetius, AntjeMeasuredat light irradiance 370 [µmol photons/m**2/s]
24Carbon uptake rate, fractionated, normalized to chlorophyll aC upt rate frac norm chl amg/mg/hBoetius, AntjeMeasuredat light irradiance 150 [µmol photons/m**2/s]
25Carbon uptake rate, fractionated, normalized to chlorophyll aC upt rate frac norm chl amg/mg/hBoetius, AntjeMeasuredat light irradiance 90 [µmol photons/m**2/s]
26Carbon uptake rate, fractionated, normalized to chlorophyll aC upt rate frac norm chl amg/mg/hBoetius, AntjeMeasuredat light irradiance 65 [µmol photons/m**2/s]
27Carbon uptake rate, fractionated, normalized to chlorophyll aC upt rate frac norm chl amg/mg/hBoetius, AntjeMeasuredat light irradiance 50 [µmol photons/m**2/s]
28Carbon uptake rate, fractionated, normalized to chlorophyll aC upt rate frac norm chl amg/mg/hBoetius, AntjeMeasuredat light irradiance 25 [µmol photons/m**2/s]
29Carbon uptake rate, fractionated, normalized to chlorophyll aC upt rate frac norm chl amg/mg/hBoetius, AntjeMeasuredat light irradiance 13 [µmol photons/m**2/s]
30Carbon uptake rate, fractionated, normalized to chlorophyll aC upt rate frac norm chl amg/mg/hBoetius, AntjeMeasuredat light irradiance 8 [µmol photons/m**2/s]
31Carbon uptake rate, fractionated, normalized to chlorophyll aC upt rate frac norm chl amg/mg/hBoetius, AntjeMeasuredat light irradiance 0 [µmol photons/m**2/s]
32ParameterParameterarbitrary unitsBoetius, AntjeCalculatedPb (Photoinhibition) [mg C/mg Chl a/h]
33ParameterParameterarbitrary unitsBoetius, AntjeCalculatedInitial slope alpha [mg C/mg Chl a/(µmol photons/m**2/s)/h]
34ParameterParameterarbitrary unitsBoetius, AntjeCalculatedPhotoinhibition [mg C/mg Chl a/(µmol photons/m**2/s)/h]
35ParameterParameterarbitrary unitsBoetius, AntjeCalculatedIm (light intensity at which photosynthesis is maximal) [µmol photons/m**2/s]
36ParameterParameterarbitrary unitsBoetius, AntjeCalculatedPbm (carbon fixation rate at that maximal irradiance) [mg C/mg Chl a/h]
37ParameterParameterarbitrary unitsBoetius, AntjeCalculatedAdaptation parameter Ik (or photoacclimation index calculated according to Platt et al., 1982) [µmol photons/m**2/s]
38ParameterParameterarbitrary unitsBoetius, AntjeCalculatedR**2
Size:
1306 data points

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