Fernández-Méndez, Mar; Peeken, Ilka; Boetius, Antje (2014): Carbon uptake rate calculated for CTD water samples during POLARSTERN cruise ARK-XXVII/3 (IceArc) in 2012 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.834217, In supplement to: Fernández-Méndez, Mar; Katlein, Christian; Rabe, Benjamin; Nicolaus, Marcel; Peeken, Ilka; Bakker, Karel; Flores, Hauke; Boetius, Antje (2015): Photosynthetic production in the central Arctic Ocean during the record sea-ice minimum in 2012. Biogeosciences, 12, 3525-3549, https://doi.org/10.5194/bg-12-3525-2015
Always quote citation above when using data! You can download the citation in several formats below.
Abstract:
Net Primary Production was measured using the 14**C uptake method with minor modifications. Seawater samples were spiked with 0.1µCi ml**-1 of 14**C labelled sodium bicarbonate (Moravek Biochemicals, Brea, USA) and distributed in 10 clear bottles (20 ml each). Subsequently they were incubated for 12 h at -1.3°C under different scalar irradiances (0-420 µmol photons m**-2 s**-1) measured with a spherical sensor (Spherical Micro Quantum Sensor US-SQS/L, Heinz Walz, Effeltrich, Germany). At the end of the incubation, samples were filtered onto 0.2 µm nitrocellulose filters and the particulate radioactive carbon uptake was determined by liquid scintillation counting using Filter count scintillation cocktail (Perkin Elmer, Waltham, USA). The carbon uptake values in the dark were subtracted from the carbon uptake values measured in the light incubations. Dissolved inorganic carbon (DIC) was measured for each sample using the flow injection system (Hall and Aller, 1992). The DIC concentration was taken into account to calculate the amount of labeled bicarbonate incorporated into the cell. Carbon fixation rates were normalized volumetrically and by chlorophyll a. Photosynthesis-irradiance curves (PI curves) were fitted using MATLAB® according to the equation proposed by Platt et al. (1980) including a photoinhibition parameter (beta) and providing the main photosynthetic parameters: maximum Chla normalized carbon fixation rate if there were no photoinhibition (Pb) and the initial slope of the saturation curve (alpha). The derived parameters: light intensity at which photosynthesis is maximal (Im), the carbon fixation rate at that maximal irradiance (Pbm) and the adaptation parameter or photoacclimation index (Ik) were calculated according to Platt et al. (1982).
Source:
Bakker, Karel (2014): Nutrients measured on water bottle samples during POLARSTERN cruise ARK-XXVII/3 (IceArc) in 2012 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.834081
Project(s):
Funding:
Seventh Framework Programme (FP7), grant/award no. 294757: Assessment of bacterial life and matter cycling in deep-sea surface sediments
Coverage:
Median Latitude: 83.456230 * Median Longitude: 85.220811 * South-bound Latitude: 77.396830 * West-bound Longitude: 17.453670 * North-bound Latitude: 88.827670 * East-bound Longitude: 133.341830
Date/Time Start: 2012-08-06T07:04:00 * Date/Time End: 2012-09-29T15:10:00
Minimum DEPTH, water: 1 m * Maximum DEPTH, water: 50 m
Event(s):
PS80/209-1 * Latitude: 81.493500 * Longitude: 30.172170 * Date/Time: 2012-08-06T07:04:00 * Elevation: -709.1 m * Location: Arctic Ocean * Campaign: ARK-XXVII/3 (PS80 IceArc) * Basis: Polarstern * Method/Device: CTD/Rosette (CTD-RO) * Comment: 32.1 708 m; Position sensor: Ship's GPS
PS80/213-1 * Latitude: 81.835830 * Longitude: 29.953830 * Date/Time: 2012-08-06T20:10:00 * Elevation: -3151.7 m * Location: Arctic Ocean * Campaign: ARK-XXVII/3 (PS80 IceArc) * Basis: Polarstern * Method/Device: CTD/Rosette (CTD-RO) * Comment: 3202 32.1; Position sensor: Ship's GPS
PS80/215-1 * Latitude: 82.495170 * Longitude: 30.003000 * Date/Time: 2012-08-07T05:32:00 * Elevation: -3626.8 m * Location: Arctic Ocean * Campaign: ARK-XXVII/3 (PS80 IceArc) * Basis: Polarstern * Method/Device: CTD/Rosette (CTD-RO) * Comment: 3694 m, SE 32.1; Position sensor: Ship's GPS
Parameter(s):
# | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
---|---|---|---|---|---|---|
1 | Sample code/label | Sample label | ||||
2 | Optional event label | Event 2 | ||||
3 | Gear | Gear | ||||
4 | Description | Description | ||||
5 | Latitude of event | Latitude | ||||
6 | Longitude of event | Longitude | ||||
7 | Date/Time of event | Date/Time | ||||
8 | Date/time start | Date/time start | ||||
9 | Date/time end | Date/time end | ||||
10 | Comment | Comment | ||||
11 | DEPTH, water | Depth water | m | Geocode | ||
12 | Carbon uptake rate, fractionated | C upt rate frac | µg/l/day | Boetius, Antje | Measured | at light irradiance 420 [µmol photons/m**2/s] |
13 | Carbon uptake rate, fractionated | C upt rate frac | µg/l/day | Boetius, Antje | Measured | at light irradiance 370 [µmol photons/m**2/s] |
14 | Carbon uptake rate, fractionated | C upt rate frac | µg/l/day | Boetius, Antje | Measured | at light irradiance 150 [µmol photons/m**2/s] |
15 | Carbon uptake rate, fractionated | C upt rate frac | µg/l/day | Boetius, Antje | Measured | at light irradiance 90 [µmol photons/m**2/s] |
16 | Carbon uptake rate, fractionated | C upt rate frac | µg/l/day | Boetius, Antje | Measured | at light irradiance 65 [µmol photons/m**2/s] |
17 | Carbon uptake rate, fractionated | C upt rate frac | µg/l/day | Boetius, Antje | Measured | at light irradiance 50 [µmol photons/m**2/s] |
18 | Carbon uptake rate, fractionated | C upt rate frac | µg/l/day | Boetius, Antje | Measured | at light irradiance 25 [µmol photons/m**2/s] |
19 | Carbon uptake rate, fractionated | C upt rate frac | µg/l/day | Boetius, Antje | Measured | at light irradiance 13 [µmol photons/m**2/s] |
20 | Carbon uptake rate, fractionated | C upt rate frac | µg/l/day | Boetius, Antje | Measured | at light irradiance 8 [µmol photons/m**2/s] |
21 | Carbon uptake rate, fractionated | C upt rate frac | µg/l/day | Boetius, Antje | Measured | at light irradiance 0 [µmol photons/m**2/s] |
22 | Carbon uptake rate, fractionated per chlorophyll a | C upt rate frac norm chl a | mg/mg/h | Boetius, Antje | Measured | at light irradiance 420 [µmol photons/m**2/s] |
23 | Carbon uptake rate, fractionated per chlorophyll a | C upt rate frac norm chl a | mg/mg/h | Boetius, Antje | Measured | at light irradiance 370 [µmol photons/m**2/s] |
24 | Carbon uptake rate, fractionated per chlorophyll a | C upt rate frac norm chl a | mg/mg/h | Boetius, Antje | Measured | at light irradiance 150 [µmol photons/m**2/s] |
25 | Carbon uptake rate, fractionated per chlorophyll a | C upt rate frac norm chl a | mg/mg/h | Boetius, Antje | Measured | at light irradiance 90 [µmol photons/m**2/s] |
26 | Carbon uptake rate, fractionated per chlorophyll a | C upt rate frac norm chl a | mg/mg/h | Boetius, Antje | Measured | at light irradiance 65 [µmol photons/m**2/s] |
27 | Carbon uptake rate, fractionated per chlorophyll a | C upt rate frac norm chl a | mg/mg/h | Boetius, Antje | Measured | at light irradiance 50 [µmol photons/m**2/s] |
28 | Carbon uptake rate, fractionated per chlorophyll a | C upt rate frac norm chl a | mg/mg/h | Boetius, Antje | Measured | at light irradiance 25 [µmol photons/m**2/s] |
29 | Carbon uptake rate, fractionated per chlorophyll a | C upt rate frac norm chl a | mg/mg/h | Boetius, Antje | Measured | at light irradiance 13 [µmol photons/m**2/s] |
30 | Carbon uptake rate, fractionated per chlorophyll a | C upt rate frac norm chl a | mg/mg/h | Boetius, Antje | Measured | at light irradiance 8 [µmol photons/m**2/s] |
31 | Carbon uptake rate, fractionated per chlorophyll a | C upt rate frac norm chl a | mg/mg/h | Boetius, Antje | Measured | at light irradiance 0 [µmol photons/m**2/s] |
32 | Parameter | Parameter | arbitrary units | Boetius, Antje | Calculated | Pb (Photoinhibition) [mg C/mg Chl a/h] |
33 | Parameter | Parameter | arbitrary units | Boetius, Antje | Calculated | Initial slope alpha [mg C/mg Chl a/(µmol photons/m**2/s)/h] |
34 | Parameter | Parameter | arbitrary units | Boetius, Antje | Calculated | Photoinhibition [mg C/mg Chl a/(µmol photons/m**2/s)/h] |
35 | Parameter | Parameter | arbitrary units | Boetius, Antje | Calculated | Im (light intensity at which photosynthesis is maximal) [µmol photons/m**2/s] |
36 | Parameter | Parameter | arbitrary units | Boetius, Antje | Calculated | Pbm (carbon fixation rate at that maximal irradiance) [mg C/mg Chl a/h] |
37 | Parameter | Parameter | arbitrary units | Boetius, Antje | Calculated | Adaptation parameter Ik (or photoacclimation index calculated according to Platt et al., 1982) [µmol photons/m**2/s] |
38 | Parameter | Parameter | arbitrary units | Boetius, Antje | Calculated | R**2 |
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Size:
1306 data points