Citation:

jpy.12054

Name: Effects of pCO2 and iron on the elemental composition and cell geometry of the marine diatom Pseudo-nitzschia pseudodelicatissima//(Bacillariophyceae)
Published: 2013
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Micro-nutrients; North Pacific; Ochrophyta; Pelagos; Phytoplankton; Pseudo-nitzschia pseudodelicatissima; Single species; Temperate

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Abstract:

Partial pressure of CO2 (pCO2) and iron availability in seawater show corresponding changes due to biological and anthropogenic activities. The simultaneous change in these factors precludes an understanding of their independent effects on the ecophysiology of phytoplankton. In addition, there is a lack of data regarding the interactive effects of these factors on phytoplankton cellular stoichiometry, which is a key driving factor for the biogeochemical cycling of oceanic nutrients. Here, we investigated the effects of pCO2 and iron availability on the elemental composition (C, N, P, and Si) of the diatom Pseudo-nitzschia pseudodelicatissima (Hasle) Hasle by dilute batch cultures under 4 pCO2 (~200, ~380, ~600, and ~800 µatm) and five dissolved inorganic iron (Fe'; ~5, ~10, ~20, ~50, and ~100 pmol /L) conditions. Our experimental procedure successfully overcame the problems associated with simultaneous changes in pCO2 and Fe' by independently manipulating carbonate chemistry and iron speciation, which allowed us to evaluate the individual effects of pCO2 and iron availability. We found that the C:N ratio decreased significantly only with an increase in Fe', whereas the C:P ratio increased significantly only with an increase in pCO2. Both Si:C and Si:N ratios decreased with increasing pCO2 and Fe'. Our results indicate that changes in pCO2 and iron availability could influence the biogeochemical cycling of nutrients in future oceans with high- CO2 levels, and, similarly, during the time course of phytoplankton blooms. Moreover, pCO2 and iron availability may also have affected oceanic nutrient biogeochemistry in the past, as these conditions have changed markedly over the Earth's history.

Keyword(s):

Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Micro-nutrients; North Pacific; Ochrophyta; Pelagos; Phytoplankton; Pseudo-nitzschia pseudodelicatissima; Single species; Temperate

Further details:

Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 34.770000 * Longitude: 134.700000

Event(s):

Harima_Nada * Latitude: 34.770000 * Longitude: 134.700000 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-07-07.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Sugie, Koji
2	Treatment	Treat		Sugie, Koji
3	Identification	ID		Sugie, Koji
4	Abundance per volume	Abund v	#/ml	Sugie, Koji
5	Growth rate	µ	1/day	Sugie, Koji
6	Cell biovolume	Cell biovol	µm³	Sugie, Koji
7	Area	Area	µm²	Sugie, Koji		surface
8	Phosphorus, particulate	PP	µmol/l	Sugie, Koji
9	Biogenic silica	bSiO2	µmol/l	Sugie, Koji
10	Nitrogen, total, particulate	TPN	µmol/l	Sugie, Koji
11	Carbon, organic, particulate	POC	µmol/l	Sugie, Koji
12	Iron, dissolved, inorganic	Fe´ diss	pmol/l	Sugie, Koji		at the start of experiment
13	Iron, dissolved, inorganic	Fe´ diss	pmol/l	Sugie, Koji		at the end of experiment
14	Iron, dissolved, inorganic	Fe´ diss	pmol/l	Sugie, Koji		mean
15	Salinity	Sal		Sugie, Koji
16	Temperature, water	Temp	°C	Sugie, Koji
17	Irradiance	E	µmol/m²/s	Sugie, Koji
18	Nitrate and Nitrite	[NO3]- + [NO2]-	µmol/l	Sugie, Koji
19	Phosphate	[PO4]3-	µmol/l	Sugie, Koji
20	Silicate	Si(OH)4	µmol/l	Sugie, Koji
21	Alkalinity, total	AT	µmol/kg	Sugie, Koji	Potentiometric titration	at the start of experiment
22	Carbon, inorganic, dissolved	DIC	µmol/kg	Sugie, Koji	Coulometric titration	at the start of experiment
23	pH	pH		Sugie, Koji	Calculated	total scale,at the start of experiment
24	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Sugie, Koji	Calculated	at the start of experiment
25	Alkalinity, total	AT	µmol/kg	Sugie, Koji	Potentiometric titration	at the end of experiment
26	Carbon, inorganic, dissolved	DIC	µmol/kg	Sugie, Koji	Coulometric titration	at the end of experiment
27	pH	pH		Sugie, Koji	Calculated	total scale, at the end of experiment
28	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Sugie, Koji	Calculated	at the end of experiment
29	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale, at the start of experiment
31	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the start of experiment
32	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the start of experiment
33	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the start of experiment
34	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the start of experiment
35	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the start of experiment
36	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the start of experiment
37	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the start of experiment
38	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale, at the end of experiment
39	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the end of experiment
40	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the end of experiment
41	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the end of experiment
42	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the end of experiment
43	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the end of experiment
44	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the end of experiment
45	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the end of experiment

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1800 data points

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