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Sugie, Koji; Yoshimura, T (2013): Effects of pCO2 and iron on the elemental composition and cell geometry of the marine diatom Pseudo-nitzschia pseudodelicatissima//(Bacillariophyceae) [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.833852, Supplement to: Sugie, K; Yoshimura, T (2013): Effects of pCO2 and iron on the elemental composition and cell geometry of the marine diatom Pseudo-nitzschia pseudodelicatissima (Bacillariophyceae). Journal of Phycology, 49(3), 475-488, https://doi.org/10.1111/jpy.12054

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Abstract:
Partial pressure of CO2 (pCO2) and iron availability in seawater show corresponding changes due to biological and anthropogenic activities. The simultaneous change in these factors precludes an understanding of their independent effects on the ecophysiology of phytoplankton. In addition, there is a lack of data regarding the interactive effects of these factors on phytoplankton cellular stoichiometry, which is a key driving factor for the biogeochemical cycling of oceanic nutrients. Here, we investigated the effects of pCO2 and iron availability on the elemental composition (C, N, P, and Si) of the diatom Pseudo-nitzschia pseudodelicatissima (Hasle) Hasle by dilute batch cultures under 4 pCO2 (~200, ~380, ~600, and ~800 µatm) and five dissolved inorganic iron (Fe'; ~5, ~10, ~20, ~50, and ~100 pmol /L) conditions. Our experimental procedure successfully overcame the problems associated with simultaneous changes in pCO2 and Fe' by independently manipulating carbonate chemistry and iron speciation, which allowed us to evaluate the individual effects of pCO2 and iron availability. We found that the C:N ratio decreased significantly only with an increase in Fe', whereas the C:P ratio increased significantly only with an increase in pCO2. Both Si:C and Si:N ratios decreased with increasing pCO2 and Fe'. Our results indicate that changes in pCO2 and iron availability could influence the biogeochemical cycling of nutrients in future oceans with high- CO2 levels, and, similarly, during the time course of phytoplankton blooms. Moreover, pCO2 and iron availability may also have affected oceanic nutrient biogeochemistry in the past, as these conditions have changed markedly over the Earth's history.
Keyword(s):
Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Micro-nutrients; North Pacific; Ochrophyta; Pelagos; Phytoplankton; Pseudo-nitzschia pseudodelicatissima; Single species; Temperate
Further details:
Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0 [webpage]. https://cran.r-project.org/package=seacarb
Coverage:
Latitude: 34.770000 * Longitude: 134.700000
Event(s):
Harima_Nada * Latitude: 34.770000 * Longitude: 134.700000 * Method/Device: Experiment (EXP)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-07-07.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1SpeciesSpeciesSugie, Koji
2TreatmentTreatSugie, Koji
3IdentificationIDSugie, Koji
4Abundance per volumeAbund v#/mlSugie, Koji
5Growth rateµ1/daySugie, Koji
6Cell biovolumeCell biovolµm3Sugie, Koji
7AreaAreaµm2Sugie, Kojisurface
8Phosphorus, particulatePPµmol/lSugie, Koji
9Biogenic silicabSiO2µmol/lSugie, Koji
10Nitrogen, total, particulateTPNµmol/lSugie, Koji
11Carbon, organic, particulatePOCµmol/lSugie, Koji
12Iron, dissolved, inorganicFe´ disspmol/lSugie, Kojiat the start of experiment
13Iron, dissolved, inorganicFe´ disspmol/lSugie, Kojiat the end of experiment
14Iron, dissolved, inorganicFe´ disspmol/lSugie, Kojimean
15SalinitySalSugie, Koji
16Temperature, waterTemp°CSugie, Koji
17IrradianceEµmol/m2/sSugie, Koji
18Nitrate and Nitrite[NO3]- + [NO2]-µmol/lSugie, Koji
19Phosphate[PO4]3-µmol/lSugie, Koji
20SilicateSi(OH)4µmol/lSugie, Koji
21Alkalinity, totalATµmol/kgSugie, KojiPotentiometric titrationat the start of experiment
22Carbon, inorganic, dissolvedDICµmol/kgSugie, KojiCoulometric titrationat the start of experiment
23pH, total scalepHTSugie, KojiCalculatedtotal scale,at the start of experiment
24Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmSugie, KojiCalculatedat the start of experiment
25Alkalinity, totalATµmol/kgSugie, KojiPotentiometric titrationat the end of experiment
26Carbon, inorganic, dissolvedDICµmol/kgSugie, KojiCoulometric titrationat the end of experiment
27pH, total scalepHTSugie, KojiCalculatedtotal scale, at the end of experiment
28Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmSugie, KojiCalculatedat the end of experiment
29Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
30pH, total scalepHTYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale, at the start of experiment
31Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)at the start of experiment
32Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)at the start of experiment
33Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)at the start of experiment
34Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)at the start of experiment
35Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)at the start of experiment
36Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)at the start of experiment
37Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)at the start of experiment
38pH, total scalepHTYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale, at the end of experiment
39Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)at the end of experiment
40Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)at the end of experiment
41Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)at the end of experiment
42Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)at the end of experiment
43Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)at the end of experiment
44Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)at the end of experiment
45Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)at the end of experiment
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1800 data points

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