Citation:

jpy.12054

Name: Effects of pCO2 and iron on the elemental composition and cell geometry of the marine diatom Pseudo-nitzschia pseudodelicatissima//(Bacillariophyceae)
Published: 2013
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Micro-nutrients; North Pacific; Ochrophyta; Pelagos; Phytoplankton; Pseudo-nitzschia pseudodelicatissima; Single species; Temperate

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Abstract:

Partial pressure of CO2 (pCO2) and iron availability in seawater show corresponding changes due to biological and anthropogenic activities. The simultaneous change in these factors precludes an understanding of their independent effects on the ecophysiology of phytoplankton. In addition, there is a lack of data regarding the interactive effects of these factors on phytoplankton cellular stoichiometry, which is a key driving factor for the biogeochemical cycling of oceanic nutrients. Here, we investigated the effects of pCO2 and iron availability on the elemental composition (C, N, P, and Si) of the diatom Pseudo-nitzschia pseudodelicatissima (Hasle) Hasle by dilute batch cultures under 4 pCO2 (~200, ~380, ~600, and ~800 µatm) and five dissolved inorganic iron (Fe'; ~5, ~10, ~20, ~50, and ~100 pmol /L) conditions. Our experimental procedure successfully overcame the problems associated with simultaneous changes in pCO2 and Fe' by independently manipulating carbonate chemistry and iron speciation, which allowed us to evaluate the individual effects of pCO2 and iron availability. We found that the C:N ratio decreased significantly only with an increase in Fe', whereas the C:P ratio increased significantly only with an increase in pCO2. Both Si:C and Si:N ratios decreased with increasing pCO2 and Fe'. Our results indicate that changes in pCO2 and iron availability could influence the biogeochemical cycling of nutrients in future oceans with high- CO2 levels, and, similarly, during the time course of phytoplankton blooms. Moreover, pCO2 and iron availability may also have affected oceanic nutrient biogeochemistry in the past, as these conditions have changed markedly over the Earth's history.

Keyword(s):

Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Micro-nutrients; North Pacific; Ochrophyta; Pelagos; Phytoplankton; Pseudo-nitzschia pseudodelicatissima; Single species; Temperate

Further details:

Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 34.770000 * Longitude: 134.700000

Event(s):

Harima_Nada * Latitude: 34.770000 * Longitude: 134.700000 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-07-07.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Sugie, Koji
2	Treatment	Treat		Sugie, Koji
3	Identification	ID		Sugie, Koji
4	Abundance per volume	Abund v	#/ml	Sugie, Koji
5	Growth rate	µ	1/day	Sugie, Koji
6	Cell biovolume	Cell biovol	µm³	Sugie, Koji
7	Area	Area	µm²	Sugie, Koji		surface
8	Phosphorus, particulate	PP	µmol/l	Sugie, Koji
9	Biogenic silica	bSiO2	µmol/l	Sugie, Koji
10	Nitrogen, total, particulate	TPN	µmol/l	Sugie, Koji
11	Carbon, organic, particulate	POC	µmol/l	Sugie, Koji
12	Iron, dissolved, inorganic	Fe´ diss	pmol/l	Sugie, Koji		at the start of experiment
13	Iron, dissolved, inorganic	Fe´ diss	pmol/l	Sugie, Koji		at the end of experiment
14	Iron, dissolved, inorganic	Fe´ diss	pmol/l	Sugie, Koji		mean
15	Salinity	Sal		Sugie, Koji
16	Temperature, water	Temp	°C	Sugie, Koji
17	Irradiance	E	µmol/m²/s	Sugie, Koji
18	Nitrate and Nitrite	[NO3]- + [NO2]-	µmol/l	Sugie, Koji
19	Phosphate	[PO4]3-	µmol/l	Sugie, Koji
20	Silicate	Si(OH)4	µmol/l	Sugie, Koji
21	Alkalinity, total	AT	µmol/kg	Sugie, Koji	Potentiometric titration	at the start of experiment
22	Carbon, inorganic, dissolved	DIC	µmol/kg	Sugie, Koji	Coulometric titration	at the start of experiment
23	pH	pH		Sugie, Koji	Calculated	total scale,at the start of experiment
24	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Sugie, Koji	Calculated	at the start of experiment
25	Alkalinity, total	AT	µmol/kg	Sugie, Koji	Potentiometric titration	at the end of experiment
26	Carbon, inorganic, dissolved	DIC	µmol/kg	Sugie, Koji	Coulometric titration	at the end of experiment
27	pH	pH		Sugie, Koji	Calculated	total scale, at the end of experiment
28	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Sugie, Koji	Calculated	at the end of experiment
29	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale, at the start of experiment
31	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the start of experiment
32	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the start of experiment
33	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the start of experiment
34	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the start of experiment
35	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the start of experiment
36	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the start of experiment
37	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the start of experiment
38	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale, at the end of experiment
39	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the end of experiment
40	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the end of experiment
41	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the end of experiment
42	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the end of experiment
43	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the end of experiment
44	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the end of experiment
45	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	at the end of experiment

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1800 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Species	2 Treat	3 ID	4 Abund v [#/ml]	5 µ [1/day]	6 Cell biovol [µm³]	7 Area [µm²] (surface)	8 PP [µmol/l]	9 bSiO2 [µmol/l]	10 TPN [µmol/l]	11 POC [µmol/l]	12 Fe´ diss [pmol/l] (at the start of experiment)	13 Fe´ diss [pmol/l] (at the end of experiment)	14 Fe´ diss [pmol/l] (mean)	15 Sal	16 Temp [°C]	17 E [µmol/m²/s]	18 [NO3]- + [NO2]- [µmol/l]	19 [PO4]3- [µmol/l]	20 Si(OH)4 [µmol/l]	21 AT [µmol/kg] (at the start of experiment, P...)	22 DIC [µmol/kg] (at the start of experiment, C...)	23 pH (total scale,at the start of e...)	24 pCO2water_SST_wet [µatm] (at the start of experiment, C...)	25 AT [µmol/kg] (at the end of experiment, Pot...)	26 DIC [µmol/kg] (at the end of experiment, Cou...)	27 pH (total scale, at the end of ex...)	28 pCO2water_SST_wet [µatm] (at the end of experiment, Cal...)	29 CSC flag (Calculated using seacarb afte...)	30 pH (total scale, at the start of ...)	31 CO2 [µmol/kg] (at the start of experiment, C...)	32 pCO2water_SST_wet [µatm] (at the start of experiment, C...)	33 fCO2water_SST_wet [µatm] (at the start of experiment, C...)	34 [HCO3]- [µmol/kg] (at the start of experiment, C...)	35 [CO3]2- [µmol/kg] (at the start of experiment, C...)	36 Omega Arg (at the start of experiment, C...)	37 Omega Cal (at the start of experiment, C...)	38 pH (total scale, at the end of ex...)	39 CO2 [µmol/kg] (at the end of experiment, Cal...)	40 pCO2water_SST_wet [µatm] (at the end of experiment, Cal...)	41 fCO2water_SST_wet [µatm] (at the end of experiment, Cal...)	42 [HCO3]- [µmol/kg] (at the end of experiment, Cal...)	43 [CO3]2- [µmol/kg] (at the end of experiment, Cal...)	44 Omega Arg (at the end of experiment, Cal...)	45 Omega Cal (at the end of experiment, Cal...)
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 171 ppm	171Fe1-1	40000.000	1.302	77.02	131.2	0.335	9.4	5.76	36.53	4.09	6.14	5.1	34.2	20	100	101	3.5	150	2336.52	1952.67	8.246	233.2	2328.43	1888.49	8.328	182.0	15	8.26	7	223	222	1677	269	4.20	6.46	8.34	6	173	173	1577	306	4.77	7.35
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 171 ppm	171Fe1-2	40833.333	1.284	77.02	131.2	0.493	9.9	6.60	41.87	3.65	6.42	5.0	34.2	20	100	101	3.5	150	2334.09	1966.27	8.223	249.3	2328.17	1881.90	8.337	177.0	15	8.24	8	239	238	1700	258	4.03	6.21	8.35	5	169	168	1567	310	4.84	7.45
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 171 ppm	171Fe2-1	37083.333	1.435	80.35	134.1	0.463	10.3	7.47	48.19	7.18	14.19	10.7	34.2	20	100	101	3.5	150	2337.98	1957.33	8.242	236.7	2351.63	1871.92	8.378	158.1	15	8.25	7	226	226	1683	267	4.16	6.41	8.39	5	150	150	1534	333	5.20	8.01
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 171 ppm	171Fe2-2	36562.500	1.503	80.35	134.1	0.511	10.8	7.43	46.44	6.60	12.50	9.5	34.2	20	100	101	3.5	150	2338.92	1969.65	8.225	249.0	2324.08	1866.74	8.353	168.4	15	8.24	8	238	237	1703	259	4.05	6.23	8.37	5	160	160	1545	317	4.95	7.62
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 171 ppm	171Fe3-1	39270.833	1.611	80.84	134.8	0.536	11.8	7.84	47.93	18.11	28.22	23.2	34.2	20	100	101	3.5	150	2337.96	1945.63	8.259	225.0	2329.09	1875.00	8.347	171.7	15	8.27	7	215	214	1664	274	4.28	6.60	8.36	5	164	163	1555	315	4.92	7.58
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 171 ppm	171Fe3-2	38020.833	1.715	80.84	134.8	0.534	11.1	8.15	49.47	11.90	25.95	18.9	34.2	20	100	101	3.5	150	2335.24	1999.91	8.172	289.2	2322.07	1881.04	8.331	180.0	15	8.18	9	277	276	1754	237	3.70	5.69	8.34	6	172	171	1569	306	4.78	7.36
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 171 ppm	171Fe4-1	59218.750	1.770	89.93	140.0	0.788	16.2	13.82	82.23	31.98	74.32	53.2	34.2	20	100	101	3.5	150	2336.81	1959.11	8.238	239.2	2319.71	1823.76	8.406	142.8	15	8.25	7	229	228	1687	265	4.13	6.37	8.42	4	136	135	1477	342	5.34	8.23
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 171 ppm	171Fe4-2	46718.750	1.675	89.93	140.0	0.710	14.4	11.48	67.42	29.53	56.97	43.3	34.2	20	100	101	3.5	150	2336.93	1970.06	8.222	251.0	2323.40	1865.95	8.354	168.1	15	8.23	8	240	240	1705	258	4.02	6.19	8.37	5	160	160	1544	317	4.95	7.62
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 171 ppm	171Fe5-1	47291.667	1.799	85.05	138.2	0.637	14.5	11.33	67.32	58.58	111.50	85.0	34.2	20	100	101	3.5	150	2333.91	1966.46	8.223	249.8	2314.51	1859.30	8.352	168.2	15	8.24	8	239	238	1701	258	4.02	6.20	8.37	5	160	160	1539	315	4.92	7.58
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 171 ppm	171Fe5-2	39687.500	1.775	85.05	138.2	0.536	12.6	9.89	58.98	53.16	97.42	75.3	34.2	20	100	101	3.5	150	2338.61	1983.73	8.202	265.5	2319.04	1882.58	8.325	182.9	15	8.22	8	254	253	1726	250	3.90	6.01	8.34	6	174	174	1574	303	4.73	7.28
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 386 ppm	386Fe1-1	46093.750	1.152	66.81	122.1	0.237	9.4	5.57	36.68	4.05	4.57	4.3	34.2	20	100	101	3.5	150	2334.18	2063.70	8.062	393.8	2324.22	2039.43	8.089	363.9	15	8.07	12	378	376	1857	195	3.04	4.68	8.10	11	349	348	1825	203	3.18	4.89
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 386 ppm	386Fe1-2	44531.250	1.085	66.81	122.1	0.262	11.0	6.22	40.74	3.93	4.50	4.2	34.2	20	100	101	3.5	150	2338.55	2071.56	8.056	402.0	2327.37	2044.42	8.086	367.9	15	8.07	13	386	384	1867	192	3.00	4.63	8.10	11	353	352	1831	202	3.16	4.87
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 386 ppm	386Fe2-1	34791.667	1.314	67.88	122.8	0.345	10.2	6.22	39.91	7.18	9.35	8.3	34.2	20	100	101	3.5	150	2336.44	2075.21	8.045	413.4	2329.64	2036.12	8.104	350.4	15	8.06	13	396	395	1874	189	2.95	4.54	8.12	11	336	335	1816	209	3.27	5.03
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 386 ppm	386Fe2-2	43645.833	1.299	67.88	122.8	0.418	9.7	6.56	43.10	7.13	9.50	8.3	34.2	20	100	101	3.5	150	2334.81	2074.56	8.044	414.8	2326.98	2031.70	8.107	346.5	15	8.06	13	398	397	1874	188	2.93	4.52	8.12	11	332	331	1811	210	3.28	5.06
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 386 ppm	386Fe3-1	50703.125	1.558	71.66	125.6	0.529	14.1	10.42	62.86	17.81	27.57	22.7	34.2	20	100	101	3.5	150	2337.33	2073.70	8.050	408.3	2320.71	2003.49	8.145	310.2	15	8.06	13	392	391	1871	190	2.97	4.58	8.16	10	297	296	1769	224	3.50	5.40
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 386 ppm	386Fe3-2	50885.417	1.565	71.66	125.6	0.539	14.8	10.49	64.21	18.33	26.59	22.5	34.2	20	100	101	3.5	150	2337.42	2070.20	8.056	400.9	2318.52	2006.13	8.138	316.7	15	8.07	12	385	384	1865	193	3.01	4.63	8.15	10	304	303	1775	221	3.45	5.32
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 386 ppm	386Fe4-1	99218.750	1.825	68.29	122.1	1.317	23.0	20.67	119.12	33.23	84.59	58.9	34.2	20	100	101	3.5	150	2335.92	2079.78	8.037	422.4	2316.47	1941.87	8.237	237.4	15	8.05	13	407	405	1881	185	2.89	4.46	8.25	7	228	227	1673	262	4.09	6.30
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 386 ppm	386Fe4-2	86015.625	1.778	68.29	122.1	1.173	21.7	18.40	108.55	35.63	80.03	57.8	34.2	20	100	101	3.5	150	2338.30	2073.44	8.053	405.2	2373.08	1999.82	8.226	252.0	15	8.06	13	390	389	1870	191	2.98	4.60	8.24	8	242	241	1729	263	4.11	6.33
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 386 ppm	386Fe5-1	89375.000	1.839	87.51	139.0	0.957	19.9	19.57	113.13	72.10	169.50	120.8	34.2	20	100	101	3.5	150	2337.63	2070.86	8.057	400.9	2313.92	1938.76	8.239	236.2	15	8.07	13	386	385	1866	192	3.00	4.62	8.25	7	227	226	1669	262	4.09	6.30
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 386 ppm	386Fe5-2	76354.167	1.824	87.51	139.0	0.894	18.9	18.02	105.43	71.01	153.40	112.2	34.2	20	100	101	3.5	150	2337.33	2072.45	8.053	404.9	2315.59	1953.77	8.218	251.3	15	8.06	13	389	388	1869	191	2.98	4.60	8.23	8	241	240	1693	253	3.95	6.09
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 614 ppm	614Fe1-1	84062.500	1.067	63.36	119.1	0.509	11.9	7.58	50.18	3.32	4.17	3.7	34.2	20	100	101	3.5	150	2337.99	2151.41	7.895	619.4	2329.21	2116.59	7.952	531.0	15	7.91	19	595	593	1990	142	2.21	3.41	7.96	17	510	508	1942	158	2.46	3.79
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 614 ppm	614Fe1-2	76718.750	1.039	63.36	119.1	0.534	12.6	7.54	49.04	3.56	4.03	3.8	34.2	20	100	101	3.5	150	2340.23	2145.47	7.913	591.8	2323.69	2115.20	7.944	540.9	15	7.93	18	568	567	1980	147	2.29	3.53	7.96	17	519	518	1943	155	2.42	3.73
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 614 ppm	614Fe2-1	59531.250	1.331	67.11	121.1	0.511	12.9	9.09	59.51	6.79	9.13	8.0	34.2	20	100	101	3.5	150	2338.34	2146.06	7.908	599.3	2320.32	2094.39	7.980	490.2	15	7.92	19	576	574	1982	145	2.27	3.50	7.99	15	471	469	1913	166	2.59	3.99
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 614 ppm	614Fe2-2	52656.250	1.297	67.11	121.1	0.320	12.8	8.75	59.67	6.68	8.30	7.5	34.2	20	100	101	3.5	150	2338.39	2148.10	7.903	606.3	2324.71	2109.76	7.957	522.2	15	7.92	19	582	580	1985	144	2.25	3.47	7.97	16	502	500	1934	159	2.48	3.82
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 614 ppm	614Fe3-1	78645.833	1.672	69.44	123.0	0.657	20.5	14.65	89.54	17.29	26.17	21.7	34.2	20	100	101	3.5	150	2338.41	2141.62	7.918	583.7	2315.11	2070.72	8.017	442.2	15	7.93	18	561	559	1975	148	2.31	3.56	8.03	14	425	423	1880	177	2.77	4.26
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 614 ppm	614Fe3-2	66354.167	1.610	69.44	123.0	0.554	18.0	13.67	84.27	16.18	27.63	21.9	34.2	20	100	101	3.5	150	2336.03	2147.12	7.901	609.5	2320.62	2069.30	8.030	428.5	15	7.91	19	586	584	1985	143	2.24	3.45	8.04	13	412	410	1874	182	2.84	4.37
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 614 ppm	614Fe4-1	140000.000	1.917	79.91	133.6	1.161	27.4	23.43	139.77	32.84	74.60	53.7	34.2	20	100	101	3.5	150	2342.78	2151.34	7.907	601.8	2315.34	2027.02	8.099	353.3	15	7.92	19	581	579	1988	145	2.26	3.49	8.11	11	340	339	1811	205	3.21	4.94
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 614 ppm	614Fe4-2	122500.000	1.908	79.91	133.6	0.876	29.0	24.38	144.17	33.73	68.19	51.0	34.2	20	100	101	3.5	150	2339.38	2145.03	7.914	590.6	2317.78	2040.44	8.079	373.7	15	7.92	18	569	568	1980	147	2.29	3.53	8.09	12	360	359	1830	198	3.10	4.77
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 614 ppm	614Fe5-1	133125.000	1.994	84.03	135.9	0.801	23.6	22.75	131.13	64.19	124.10	94.1	34.2	20	100	101	3.5	150	2340.93	2151.93	7.902	609.7	2316.08	2050.16	8.058	395.2	15	7.91	19	588	586	1989	143	2.24	3.45	8.07	12	381	379	1847	191	2.98	4.59
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 614 ppm	614Fe5-2	113125.000	1.947	84.03	135.9	1.005	23.0	21.01	126.73	67.34	152.00	109.7	34.2	20	100	101	3.5	150	2338.97	2144.48	7.914	590.6	2314.23	2023.29	8.103	348.9	15	7.92	18	569	567	1979	147	2.29	3.53	8.11	11	335	334	1805	207	3.23	4.98
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 795 ppm	795Fe1-1	103593.750	1.116	67.15	123.1	0.514	14.2	9.74	63.47	3.23	3.69	3.5	34.2	20	100	101	3.5	150	2333.09	2181.96	7.814	764.1	2329.36	2163.04	7.850	694.0	15	7.83	24	734	732	2038	121	1.88	2.90	7.86	22	667	664	2012	129	2.02	3.11
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 795 ppm	795Fe1-2	84166.667	1.086	67.15	123.1	0.476	12.4	8.41	54.55	3.17	3.63	3.4	34.2	20	100	101	3.5	150	2337.22	2188.05	7.809	776.2	2330.26	2165.86	7.846	703.0	15	7.82	24	745	743	2044	119	1.87	2.87	7.86	22	675	673	2016	128	2.00	3.09
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 795 ppm	795Fe2-1	46406.250	1.327	63.30	118.9	0.327	10.1	7.31	49.72	6.25	7.40	6.8	34.2	20	100	101	3.5	150	2334.70	2185.12	7.810	772.8	2334.05	2164.98	7.856	685.2	15	7.82	24	742	740	2041	120	1.87	2.88	7.87	21	658	656	2012	131	2.05	3.15
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 795 ppm	795Fe2-2	54531.250	1.359	63.30	118.9	0.358	11.3	7.69	51.91	6.52	7.89	7.2	34.2	20	100	101	3.5	150	2337.18	2182.55	7.822	750.3	2332.61	2156.15	7.873	655.1	15	7.83	23	720	718	2036	123	1.92	2.95	7.89	20	629	627	2000	136	2.12	3.26
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 795 ppm	795Fe3-1	71765.047	1.574	65.79	120.7	0.549	16.0	12.42	78.64	15.94	22.72	19.3	34.2	20	100	101	3.5	150	2339.35	2186.10	7.819	757.3	2330.49	2136.64	7.912	590.5	15	7.83	24	728	725	2041	122	1.90	2.93	7.92	18	567	565	1972	146	2.28	3.51
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 795 ppm	795Fe3-2	65312.500	1.518	65.79	120.7	0.546	15.1	11.93	73.13	15.65	22.65	19.2	34.2	20	100	101	3.5	150	2338.32	2187.26	7.813	767.1	2333.80	2140.09	7.911	592.5	15	7.83	24	737	735	2043	121	1.88	2.90	7.92	18	569	567	1976	146	2.28	3.51
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 795 ppm	795Fe4-1	126718.750	1.917	87.11	138.4	1.020	26.3	24.42	140.65	32.32	58.99	45.7	34.2	20	100	101	3.5	150	2338.44	2183.56	7.824	745.9	2325.01	2100.70	7.978	494.4	15	7.83	23	720	717	2037	123	1.92	2.96	7.99	15	477	475	1920	165	2.57	3.97
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 795 ppm	795Fe4-2	121354.167	1.855	87.11	138.4	1.000	23.0	22.41	131.43	31.93	69.75	50.8	34.2	20	100	101	3.5	150	2333.61	2180.31	7.821	751.3	2326.26	2081.86	8.017	445.2	15	7.83	24	724	722	2035	122	1.90	2.93	8.03	14	429	427	1890	178	2.77	4.27
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 795 ppm	795Fe5-1	85625.000	1.933	76.30	129.6	0.607	16.5	16.96	101.34	65.36	106.40	85.9	34.2	20	100	101	3.5	150	2339.07	2182.52	7.827	740.9	2325.13	2112.65	7.953	529.0	15	7.84	23	713	710	2036	124	1.93	2.98	7.96	16	509	507	1939	157	2.46	3.79
Pseudo-nitzschia pseudodelicatissima (phytoplankton)	pCO2 795 ppm	795Fe5-2	85156.250	1.919	76.30	129.6	0.707	16.5	17.34	102.23	64.31	104.50	84.4	34.2	20	100	101	3.5	150	2335.26	2180.75	7.823	748.1	2323.20	2113.01	7.948	534.8	15	7.83	23	720	717	2035	123	1.91	2.95	7.96	17	514	513	1940	156	2.43	3.75