De Vittor, Cinzia; Cerino, Federica; Coscia, Lucia; Beran, Alfred; Kralj, Martina; Comici, Cinzia; Karuza, Ana (2014): Culture experiments to evaluate CO2 effects on growth and morphology of the coccolithophore Pleurochrysis cf. pseudoroscoffensis [dataset]. Istituto Nazionale di Oceanografia e di Geofisica Sperimentale, Sgonico, PANGAEA, https://doi.org/10.1594/PANGAEA.833845
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Published: 2014-07-08 • DOI registered: 2023-03-09
Abstract:
In the framework of the Italian project CO2 Monitor, two culture experiments were carried out in vertical closed photobioreactors with Pleurochrysis cf. pseudoroscoffensis Gayral & Fresnel 1983, a coccolithophore isolated from the Gulf of Trieste (North Adriatic Sea).
The aim of this study was to investigate the effects induced by pH variations due to CO2 emissions on its growth and morphology.
Two experiments were carried out with two different CO2 concentrations (1 and 2%) relatively to the total volume of insufflated air. Growth and cell size in light microscopy, morphology and coccolith size in scanning electron microscopy, particulate nitrogen (PN) and particulate inorganic and organic carbon (PIC and POC) content of the coccolithophore were investigated during the light and dark phases. Dissolved inorganic nutrient (nitrate and phosphate) concentrations and pH of the medium and the presence of heterotrophic prokaryotes (HP) were monitored as well.
Further details:
Scanning electron micrographs of Pleurocrhysis cf. pseudoroscoffensis. hdl:10013/epic.43898.d001
Project(s):
Funding:
Seventh Framework Programme of the European Commission (FP7), grant/award no. 265847: Sub-seabed CO2 Storage: Impact on Marine Ecosystems
Coverage:
Date/Time Start: 2012-11-19T12:00:00 * Date/Time End: 2013-06-19T07:00:00
Comment:
Slight changes in morphology of coccoliths resulted from analysis of scanning electron micrographs. Coccoliths particularly showed thinner inner tubes and a lower number of nodes compared to the control in the experiment with 1% CO2.
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Treatment | Treat | De Vittor, Cinzia | |||
| 2 | DATE/TIME | Date/Time | De Vittor, Cinzia | Geocode | ||
| 3 | Day of experiment | DOE | day | De Vittor, Cinzia | ||
| 4 | Species | Species | De Vittor, Cinzia | |||
| 5 | Cell | Cell | #/ml | De Vittor, Cinzia | ||
| 6 | Length | l | µm | De Vittor, Cinzia | coccolith | |
| 7 | Width | w | µm | De Vittor, Cinzia | coccolith | |
| 8 | Length | l | µm | De Vittor, Cinzia | cell | |
| 9 | Width | w | µm | De Vittor, Cinzia | cell | |
| 10 | pH | pH | De Vittor, Cinzia | pH meter | ||
| 11 | Nitrogen, total, particulate | TPN | µmol/l | De Vittor, Cinzia | ||
| 12 | Carbon, organic, particulate | POC | µmol/l | De Vittor, Cinzia | ||
| 13 | Carbon, inorganic, particulate | PIC | µmol/l | De Vittor, Cinzia | ||
| 14 | Ammonium | [NH4]+ | µmol/l | De Vittor, Cinzia | ||
| 15 | Nitrate | [NO3]- | µmol/l | De Vittor, Cinzia | ||
| 16 | Nitrite | [NO2]- | µmol/l | De Vittor, Cinzia | ||
| 17 | Phosphate | [PO4]3- | µmol/l | De Vittor, Cinzia | ||
| 18 | Silicon dioxide | SiO2 | µmol/l | De Vittor, Cinzia | ||
| 19 | Prokaryotes, heterotroph | Prok het | #/ml | De Vittor, Cinzia |
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Size:
750 data points
Data
| 1 Treat | 2 Date/Time | 3 DOE [day] | 4 Species | 5 Cell [#/ml] | 6 l [µm] (coccolith) | 7 w [µm] (coccolith) | 8 l [µm] (cell) | 9 w [µm] (cell) | 10 pH | 11 TPN [µmol/l] | 12 POC [µmol/l] | 13 PIC [µmol/l] | 14 [NH4]+ [µmol/l] | 15 [NO3]- [µmol/l] | 16 [NO2]- [µmol/l] | 17 [PO4]3- [µmol/l] | 18 SiO2 [µmol/l] | 19 Prok het [#/ml] |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CO2Monitor_Ctrl_Exp1% | 2012-11-19T12:00 | 0 | Pleurochrysis cf. pseudoroscoffensis | 20000 | 7.17 | 7.73 | 8.1 | 283.10 | 2448.72 | 370.26 | 3.06 | 918.6500 | 0.19 | 40.0900 | 276.73 | 521574 | ||
| CO2Monitor_Ctrl_Exp1% | 2012-11-19T16:00 | 0 | Pleurochrysis cf. pseudoroscoffensis | 17047 | 9.14 | 10.02 | 8.5 | |||||||||||
| CO2Monitor_Ctrl_Exp1% | 2012-11-20T08:00 | 0 | Pleurochrysis cf. pseudoroscoffensis | 8.4 | ||||||||||||||
| CO2Monitor_Ctrl_Exp1% | 2012-11-20T16:00 | 1 | Pleurochrysis cf. pseudoroscoffensis | 31617 | 8.5 | 0.90 | 776.3300 | 2.60 | 19.4700 | 269.67 | ||||||||
| CO2Monitor_Ctrl_Exp1% | 2012-11-21T08:00 | 1 | Pleurochrysis cf. pseudoroscoffensis | 8.5 | ||||||||||||||
| CO2Monitor_Ctrl_Exp1% | 2012-11-21T17:00 | 2 | Pleurochrysis cf. pseudoroscoffensis | 95800 | 8.7 | 195.86 | 1800.26 | 277.24 | 0.52 | 654.8100 | 4.31 | 5.0500 | 266.74 | 2039172 | ||||
| CO2Monitor_Ctrl_Exp1% | 2012-11-22T08:00 | 2 | Pleurochrysis cf. pseudoroscoffensis | 129532 | 8.5 | 214.70 | 2027.24 | 465.94 | ||||||||||
| CO2Monitor_Ctrl_Exp1% | 2012-11-22T17:00 | 3 | Pleurochrysis cf. pseudoroscoffensis | 157891 | 2.23 | 1.47 | 7.53 | 8.17 | 8.7 | 419.21 | 3483.55 | 691.84 | 0.58 | 580.2100 | 5.19 | 0.6931 | 267.38 | |
| CO2Monitor_Ctrl_Exp1% | 2012-11-23T08:00 | 3 | Pleurochrysis cf. pseudoroscoffensis | 2.49 | 1.68 | 8.3 | ||||||||||||
| CO2Monitor_Ctrl_Exp1% | 2012-11-23T17:00 | 4 | Pleurochrysis cf. pseudoroscoffensis | 180106 | 8.7 | 396.17 | 3342.67 | 1100.38 | 0.73 | 456.6200 | 6.20 | 0.7300 | 268.13 | 1010033 | ||||
| CO2Monitor_Ctrl_Exp1% | 2012-11-24T08:00 | 4 | Pleurochrysis cf. pseudoroscoffensis | 199370 | 8.0 | |||||||||||||
| CO2Monitor_Ctrl_Exp1% | 2012-11-24T17:00 | 5 | Pleurochrysis cf. pseudoroscoffensis | 208737 | 8.7 | 0.90 | 320.0800 | 6.83 | 0.9500 | 282.40 | ||||||||
| CO2Monitor_Ctrl_Exp1% | 2012-11-25T08:00 | 5 | Pleurochrysis cf. pseudoroscoffensis | |||||||||||||||
| CO2Monitor_Ctrl_Exp1% | 2012-11-25T17:00 | 6 | Pleurochrysis cf. pseudoroscoffensis | 258818 | 8.7 | 590.63 | 5228.01 | 941.62 | 0.52 | 169.6600 | 7.17 | 0.6900 | 275.60 | 2808057 | ||||
| CO2Monitor_Ctrl_Exp1% | 2012-11-26T08:00 | 6 | Pleurochrysis cf. pseudoroscoffensis | 249070 | 7.9 | 587.80 | 5096.12 | 1097.11 | ||||||||||
| CO2Monitor_Ctrl_Exp1% | 2012-11-26T17:00 | 7 | Pleurochrysis cf. pseudoroscoffensis | 257671 | 8.8 | 612.55 | 5710.85 | 752.06 | 0.54 | 61.6650 | 7.28 | 0.6400 | 289.30 | |||||
| CO2Monitor_Ctrl_Exp1% | 2012-11-27T08:00 | 7 | Pleurochrysis cf. pseudoroscoffensis | 7.9 | 528.93 | 6289.55 | ||||||||||||
| CO2Monitor_Ctrl_Exp1% | 2012-11-27T17:00 | 8 | Pleurochrysis cf. pseudoroscoffensis | 286153 | 7.68 | 8.41 | 8.6 | 0.58 | 0.1084 | 0.13 | 0.6300 | 278.47 | ||||||
| CO2Monitor_Ctrl_Exp1% | 2012-11-28T08:00 | 8 | Pleurochrysis cf. pseudoroscoffensis | 7.8 | ||||||||||||||
| CO2Monitor_Ctrl_Exp1% | 2012-11-28T17:00 | 9 | Pleurochrysis cf. pseudoroscoffensis | 276022 | 2.13 | 1.33 | 8.47 | 8.86 | 8.5 | 620.23 | 6490.07 | 1713.75 | 0.68 | 0.0030 | 0.10 | 0.6404 | 295.37 | 1086040 |
| CO2Monitor_Ctrl_Exp1% | 2012-11-29T08:00 | 9 | Pleurochrysis cf. pseudoroscoffensis | 240086 | 2.28 | 1.45 | 7.8 | 497.16 | 6412.82 | 957.70 | ||||||||
| CO2Monitor_CO2_Exp1% | 2012-11-19T12:00 | 0 | Pleurochrysis cf. pseudoroscoffensis | 20000 | 7.17 | 7.73 | 7.6 | 283.10 | 2448.72 | 370.26 | 3.06 | 918.6500 | 0.19 | 40.0900 | 276.73 | 330669 | ||
| CO2Monitor_CO2_Exp1% | 2012-11-19T16:00 | 0 | Pleurochrysis cf. pseudoroscoffensis | 13856 | 8.73 | 9.59 | 7.7 | |||||||||||
| CO2Monitor_CO2_Exp1% | 2012-11-20T08:00 | 0 | Pleurochrysis cf. pseudoroscoffensis | 7.9 | ||||||||||||||
| CO2Monitor_CO2_Exp1% | 2012-11-20T16:00 | 1 | Pleurochrysis cf. pseudoroscoffensis | 44825 | 7.6 | 0.61 | 778.3650 | 2.54 | 16.4450 | 271.99 | ||||||||
| CO2Monitor_CO2_Exp1% | 2012-11-21T08:00 | 1 | Pleurochrysis cf. pseudoroscoffensis | 7.7 | ||||||||||||||
| CO2Monitor_CO2_Exp1% | 2012-11-21T17:00 | 2 | Pleurochrysis cf. pseudoroscoffensis | 104151 | 7.8 | 307.95 | 2389.43 | 548.71 | 1.26 | 571.6450 | 4.86 | 1.4564 | 269.60 | 586133 | ||||
| CO2Monitor_CO2_Exp1% | 2012-11-22T08:00 | 2 | Pleurochrysis cf. pseudoroscoffensis | 172318 | 7.5 | 434.07 | 2678.51 | 1411.48 | ||||||||||
| CO2Monitor_CO2_Exp1% | 2012-11-22T17:00 | 3 | Pleurochrysis cf. pseudoroscoffensis | 164906 | 2.37 | 1.55 | 7.31 | 8.10 | 7.6 | 0.71 | 331.7250 | 6.26 | 0.8381 | 278.48 | ||||
| CO2Monitor_CO2_Exp1% | 2012-11-23T08:00 | 3 | Pleurochrysis cf. pseudoroscoffensis | 2.27 | 1.40 | 7.4 | ||||||||||||
| CO2Monitor_CO2_Exp1% | 2012-11-23T17:00 | 4 | Pleurochrysis cf. pseudoroscoffensis | 309655 | 7.6 | 827.08 | 6722.30 | 1313.11 | 1.31 | 78.0029 | 1.05 | 0.9900 | 294.45 | 515392 | ||||
| CO2Monitor_CO2_Exp1% | 2012-11-24T08:00 | 4 | Pleurochrysis cf. pseudoroscoffensis | 263788 | 7.3 | 620.85 | 6774.56 | 1401.25 | ||||||||||
| CO2Monitor_CO2_Exp1% | 2012-11-24T17:00 | 5 | Pleurochrysis cf. pseudoroscoffensis | 381155 | 7.6 | 0.68 | 1.8504 | 0.07 | 0.7000 | 295.39 | ||||||||
| CO2Monitor_CO2_Exp1% | 2012-11-25T08:00 | 5 | Pleurochrysis cf. pseudoroscoffensis | |||||||||||||||
| CO2Monitor_CO2_Exp1% | 2012-11-25T17:00 | 6 | Pleurochrysis cf. pseudoroscoffensis | 432383 | 7.5 | 728.14 | 9503.96 | 2938.41 | 1.09 | 6.2944 | 0.46 | 1.1200 | 293.89 | 2609938 | ||||
| CO2Monitor_CO2_Exp1% | 2012-11-26T08:00 | 6 | Pleurochrysis cf. pseudoroscoffensis | 380199 | 7.1 | 705.38 | 8887.12 | 3282.94 | ||||||||||
| CO2Monitor_CO2_Exp1% | 2012-11-26T17:00 | 7 | Pleurochrysis cf. pseudoroscoffensis | 397403 | 7.4 | 599.15 | 10753.60 | 2353.84 | 1.18 | 0.7470 | 0.18 | 1.2300 | 293.43 | |||||
| CO2Monitor_CO2_Exp1% | 2012-11-27T08:00 | 7 | Pleurochrysis cf. pseudoroscoffensis | 7.2 | 964.63 | 11866.09 | 2674.61 | |||||||||||
| CO2Monitor_CO2_Exp1% | 2012-11-27T17:00 | 8 | Pleurochrysis cf. pseudoroscoffensis | 397403 | 8.89 | 9.40 | 7.5 | 1.93 | 0.0389 | 0.15 | 1.3200 | 321.25 | ||||||
| CO2Monitor_CO2_Exp1% | 2012-11-28T08:00 | 8 | Pleurochrysis cf. pseudoroscoffensis | 7.1 | ||||||||||||||
| CO2Monitor_CO2_Exp1% | 2012-11-28T17:00 | 9 | Pleurochrysis cf. pseudoroscoffensis | 322854 | 2.18 | 1.33 | 9.33 | 9.77 | 7.7 | 576.33 | 12173.62 | 1875.31 | 3.32 | 0.0254 | 0.13 | 2.0250 | 287.92 | 434769 |
| CO2Monitor_CO2_Exp1% | 2012-11-29T08:00 | 9 | Pleurochrysis cf. pseudoroscoffensis | 341395 | 2.14 | 1.34 | 7.2 | 622.16 | 12828.85 | 1516.57 | ||||||||
| CO2Monitor_Ctrl_Exp2% | 2013-06-10T11:00 | 0 | Pleurochrysis cf. pseudoroscoffensis | 20000 | 6.69 | 7.51 | 8.2 | 3.55 | 904.5700 | 0.08 | 36.0600 | 262.16 | 474489 | |||||
| CO2Monitor_Ctrl_Exp2% | 2013-06-10T15:00 | 0 | Pleurochrysis cf. pseudoroscoffensis | 15195 | 9.29 | 9.96 | 8.3 | 47.35 | 1309.86 | |||||||||
| CO2Monitor_Ctrl_Exp2% | 2013-06-11T07:00 | 0 | Pleurochrysis cf. pseudoroscoffensis | 21175 | 8.0 | 74.37 | 1027.55 | 380.28 | ||||||||||
| CO2Monitor_Ctrl_Exp2% | 2013-06-11T15:00 | 1 | Pleurochrysis cf. pseudoroscoffensis | 35388 | 8.3 | 1.70 | 837.3600 | 4.33 | 19.5400 | 248.97 | ||||||||
| CO2Monitor_Ctrl_Exp2% | 2013-06-12T07:00 | 1 | Pleurochrysis cf. pseudoroscoffensis | 48237 | 7.9 | |||||||||||||
| CO2Monitor_Ctrl_Exp2% | 2013-06-12T15:00 | 2 | Pleurochrysis cf. pseudoroscoffensis | 78395 | 2.28 | 1.43 | 8.4 | 234.15 | 1969.96 | 98.16 | 3.69 | 695.0800 | 6.54 | 7.9000 | 248.72 | 1539763 | ||
| CO2Monitor_Ctrl_Exp2% | 2013-06-13T07:00 | 2 | Pleurochrysis cf. pseudoroscoffensis | 126398 | 2.30 | 1.45 | 7.8 | 256.29 | 1276.65 | 1553.88 | ||||||||
| CO2Monitor_Ctrl_Exp2% | 2013-06-13T15:00 | 3 | Pleurochrysis cf. pseudoroscoffensis | 165375 | 7.20 | 7.73 | 8.6 | 5.53 | 565.9000 | 7.54 | 0.6900 | 252.97 | ||||||
| CO2Monitor_Ctrl_Exp2% | 2013-06-14T07:00 | 3 | Pleurochrysis cf. pseudoroscoffensis | 178558 | 7.7 | |||||||||||||
| CO2Monitor_Ctrl_Exp2% | 2013-06-14T15:00 | 4 | Pleurochrysis cf. pseudoroscoffensis | 253657 | 8.7 | 419.46 | 3829.64 | 612.95 | 1.39 | 396.6100 | 8.49 | 0.7800 | 253.77 | 2240642 | ||||
| CO2Monitor_Ctrl_Exp2% | 2013-06-15T07:00 | 4 | Pleurochrysis cf. pseudoroscoffensis | 222691 | 7.7 | 582.97 | 4623.72 | 1005.93 | ||||||||||
| CO2Monitor_Ctrl_Exp2% | 2013-06-15T15:00 | 5 | Pleurochrysis cf. pseudoroscoffensis | 254231 | 8.6 | 13.56 | 228.0400 | 8.16 | 1.2600 | 272.36 | ||||||||
| CO2Monitor_Ctrl_Exp2% | 2013-06-16T07:00 | 5 | Pleurochrysis cf. pseudoroscoffensis | 250599 | 7.7 | |||||||||||||
| CO2Monitor_Ctrl_Exp2% | 2013-06-16T15:00 | 6 | Pleurochrysis cf. pseudoroscoffensis | 243717 | 2.23 | 1.40 | 8.6 | 788.77 | 6583.33 | 1289.82 | 1.25 | 55.3800 | 9.10 | 0.6400 | 259.95 | 2726842 | ||
| CO2Monitor_Ctrl_Exp2% | 2013-06-17T07:00 | 6 | Pleurochrysis cf. pseudoroscoffensis | 271052 | 2.19 | 1.37 | 7.7 | 755.10 | 6880.71 | 1021.69 | ||||||||
| CO2Monitor_Ctrl_Exp2% | 2013-06-17T15:00 | 7 | Pleurochrysis cf. pseudoroscoffensis | 268567 | 8.02 | 8.49 | 8.5 | 531.58 | 5646.75 | 1099.47 | 5.57 | 12.3100 | 0.10 | 1.5400 | 265.34 | 5670771 | ||
| CO2Monitor_Ctrl_Exp2% | 2013-06-19T07:00 | 7 | Pleurochrysis cf. pseudoroscoffensis | 256907 | 7.6 | |||||||||||||
| CO2Monitor_CO2_Exp2% | 2013-06-10T11:00 | 0 | Pleurochrysis cf. pseudoroscoffensis | 20000 | 6.69 | 7.51 | 7.0 | 3.55 | 904.5700 | 0.08 | 36.0600 | 262.16 | 334933 | |||||
| CO2Monitor_CO2_Exp2% | 2013-06-10T15:00 | 0 | Pleurochrysis cf. pseudoroscoffensis | 15329 | 9.29 | 9.96 | 7.2 | 49.19 | 489.76 | 30.61 | ||||||||
| CO2Monitor_CO2_Exp2% | 2013-06-11T07:00 | 0 | Pleurochrysis cf. pseudoroscoffensis | 20132 | 7.1 | 76.58 | 888.29 | 10.45 | ||||||||||
| CO2Monitor_CO2_Exp2% | 2013-06-11T15:00 | 1 | Pleurochrysis cf. pseudoroscoffensis | 30987 | 7.1 | 1.44 | 833.8800 | 3.47 | 19.8800 | 245.01 | ||||||||
| CO2Monitor_CO2_Exp2% | 2013-06-12T07:00 | 1 | Pleurochrysis cf. pseudoroscoffensis | 64451 | 7.1 | |||||||||||||
| CO2Monitor_CO2_Exp2% | 2013-06-12T15:00 | 2 | Pleurochrysis cf. pseudoroscoffensis | 126933 | 2.30 | 1.47 | 7.1 | 267.94 | 2023.23 | 423.59 | 1.51 | 646.0200 | 5.48 | 6.3900 | 252.46 | 509378 | ||
| CO2Monitor_CO2_Exp2% | 2013-06-13T07:00 | 2 | Pleurochrysis cf. pseudoroscoffensis | 204971 | 7.2 | 390.41 | 2908.24 | 825.28 | ||||||||||
| CO2Monitor_CO2_Exp2% | 2013-06-13T15:00 | 3 | Pleurochrysis cf. pseudoroscoffensis | 215836 | 7.20 | 7.73 | 7.1 | 9.76 | 376.9200 | 7.22 | 0.6600 | 255.42 | ||||||
| CO2Monitor_CO2_Exp2% | 2013-06-14T07:00 | 3 | Pleurochrysis cf. pseudoroscoffensis | 201665 | 7.2 | |||||||||||||
| CO2Monitor_CO2_Exp2% | 2013-06-14T15:00 | 4 | Pleurochrysis cf. pseudoroscoffensis | 315399 | 7.2 | 872.28 | 5736.36 | 1683.63 | 15.21 | 72.9500 | 0.06 | 1.8100 | 252.77 | 2249307 | ||||
| CO2Monitor_CO2_Exp2% | 2013-06-15T07:00 | 4 | Pleurochrysis cf. pseudoroscoffensis | 262832 | 7.2 | 738.10 | 8219.36 | 1377.07 | ||||||||||
| CO2Monitor_CO2_Exp2% | 2013-06-15T15:00 | 5 | Pleurochrysis cf. pseudoroscoffensis | 382302 | 6.9 | 4.64 | 45.6400 | 0.19 | 1.6800 | 277.76 | ||||||||
| CO2Monitor_CO2_Exp2% | 2013-06-16T07:00 | 5 | Pleurochrysis cf. pseudoroscoffensis | 277169 | 6.9 | |||||||||||||
| CO2Monitor_CO2_Exp2% | 2013-06-16T15:00 | 6 | Pleurochrysis cf. pseudoroscoffensis | 385360 | 2.22 | 1.40 | 6.9 | 872.62 | 13102.32 | 3096.13 | 3.84 | 29.2800 | 0.14 | 1.7700 | 281.18 | 6217200 | ||
| CO2Monitor_CO2_Exp2% | 2013-06-17T07:00 | 6 | Pleurochrysis cf. pseudoroscoffensis | 292799 | 2.23 | 1.40 | 7.2 | 619.09 | 12618.56 | 820.41 | ||||||||
| CO2Monitor_CO2_Exp2% | 2013-06-17T15:00 | 7 | Pleurochrysis cf. pseudoroscoffensis | 187744 | 8.02 | 8.49 | 7.1 | 461.77 | 10820.44 | 238.13 | 13.91 | 48.7900 | 0.28 | 1.8800 | 278.85 | 6656800 | ||
| CO2Monitor_CO2_Exp2% | 2013-06-19T07:00 | 7 | Pleurochrysis cf. pseudoroscoffensis | 268949 | 7.2 |