/* DATA DESCRIPTION: Citation: De Vittor, Cinzia; Cerino, Federica; Coscia, Lucia; Beran, Alfred; Kralj, Martina; Comici, Cinzia; Karuza, Ana (2014): Culture experiments to evaluate CO2 effects on growth and morphology of the coccolithophore Pleurochrysis cf. pseudoroscoffensis [dataset]. Istituto Nazionale di Oceanografia e di Geofisica Sperimentale, Sgonico, PANGAEA, https://doi.org/10.1594/PANGAEA.833845 Abstract: In the framework of the Italian project CO2 Monitor, two culture experiments were carried out in vertical closed photobioreactors with Pleurochrysis cf. pseudoroscoffensis Gayral & Fresnel 1983, a coccolithophore isolated from the Gulf of Trieste (North Adriatic Sea). The aim of this study was to investigate the effects induced by pH variations due to CO2 emissions on its growth and morphology. Two experiments were carried out with two different CO2 concentrations (1 and 2%) relatively to the total volume of insufflated air. Growth and cell size in light microscopy, morphology and coccolith size in scanning electron microscopy, particulate nitrogen (PN) and particulate inorganic and organic carbon (PIC and POC) content of the coccolithophore were investigated during the light and dark phases. Dissolved inorganic nutrient (nitrate and phosphate) concentrations and pH of the medium and the presence of heterotrophic prokaryotes (HP) were monitored as well. Further details: Scanning electron micrographs of Pleurocrhysis cf. pseudoroscoffensis. hdl:10013/epic.43898.d001 Project(s): Sub-seabed CO2 Storage: Impact on Marine Ecosystems (ECO2) (URI: http://www.eco2-project.eu/) Funding: Seventh Framework Programme of the European Commission (FP7), grant/award no. 265847: Sub-seabed CO2 Storage: Impact on Marine Ecosystems (URI: https://cordis.europa.eu/project/id/265847) Coverage: DATE/TIME START: 2012-11-19T12:00:00 * DATE/TIME END: 2013-06-19T07:00:00 Comment: Slight changes in morphology of coccoliths resulted from analysis of scanning electron micrographs. Coccoliths particularly showed thinner inner tubes and a lower number of nodes compared to the control in the experiment with 1% CO2. Parameter(s): Treatment (Treat) * PI: De Vittor, Cinzia (https://orcid.org/0000-0001-8552-4396, cdevittor@ogs.it) DATE/TIME (Date/Time) * GEOCODE * PI: De Vittor, Cinzia (https://orcid.org/0000-0001-8552-4396, cdevittor@ogs.it) Day of experiment [day] (DOE) * PI: De Vittor, Cinzia (https://orcid.org/0000-0001-8552-4396, cdevittor@ogs.it) Species (Species) * PI: De Vittor, Cinzia (https://orcid.org/0000-0001-8552-4396, cdevittor@ogs.it) Cell [#/ml] (Cell) * PI: De Vittor, Cinzia (https://orcid.org/0000-0001-8552-4396, cdevittor@ogs.it) Length [µm] (l) * PI: De Vittor, Cinzia (https://orcid.org/0000-0001-8552-4396, cdevittor@ogs.it) * COMMENT: coccolith Width [µm] (w) * PI: De Vittor, Cinzia (https://orcid.org/0000-0001-8552-4396, cdevittor@ogs.it) * COMMENT: coccolith Length [µm] (l) * PI: De Vittor, Cinzia (https://orcid.org/0000-0001-8552-4396, cdevittor@ogs.it) * COMMENT: cell Width [µm] (w) * PI: De Vittor, Cinzia (https://orcid.org/0000-0001-8552-4396, cdevittor@ogs.it) * COMMENT: cell pH (pH) * PI: De Vittor, Cinzia (https://orcid.org/0000-0001-8552-4396, cdevittor@ogs.it) * METHOD/DEVICE: pH meter Nitrogen, total, particulate [µmol/l] (TPN) * PI: De Vittor, Cinzia (https://orcid.org/0000-0001-8552-4396, cdevittor@ogs.it) Carbon, organic, particulate [µmol/l] (POC) * PI: De Vittor, Cinzia (https://orcid.org/0000-0001-8552-4396, cdevittor@ogs.it) Carbon, inorganic, particulate [µmol/l] (PIC) * PI: De Vittor, Cinzia (https://orcid.org/0000-0001-8552-4396, cdevittor@ogs.it) Ammonium [µmol/l] ([NH4]+) * PI: De Vittor, Cinzia (https://orcid.org/0000-0001-8552-4396, cdevittor@ogs.it) Nitrate [µmol/l] ([NO3]-) * PI: De Vittor, Cinzia (https://orcid.org/0000-0001-8552-4396, cdevittor@ogs.it) Nitrite [µmol/l] ([NO2]-) * PI: De Vittor, Cinzia (https://orcid.org/0000-0001-8552-4396, cdevittor@ogs.it) Phosphate [µmol/l] ([PO4]3-) * PI: De Vittor, Cinzia (https://orcid.org/0000-0001-8552-4396, cdevittor@ogs.it) Silicon dioxide [µmol/l] (SiO2) * PI: De Vittor, Cinzia (https://orcid.org/0000-0001-8552-4396, cdevittor@ogs.it) Prokaryotes, heterotroph [#/ml] (Prok het) * PI: De Vittor, Cinzia (https://orcid.org/0000-0001-8552-4396, cdevittor@ogs.it) License: Creative Commons Attribution 3.0 Unported (CC-BY-3.0) (URI: https://creativecommons.org/licenses/by/3.0/) Size: 750 data points */ Treat Date/Time DOE [day] Species Cell [#/ml] l [µm] (coccolith) w [µm] (coccolith) l [µm] (cell) w [µm] (cell) pH TPN [µmol/l] POC [µmol/l] PIC [µmol/l] [NH4]+ [µmol/l] [NO3]- [µmol/l] [NO2]- [µmol/l] [PO4]3- [µmol/l] SiO2 [µmol/l] Prok het [#/ml] CO2Monitor_Ctrl_Exp1% 2012-11-19T12:00 0 Pleurochrysis cf. pseudoroscoffensis 20000 7.17 7.73 8.1 283.10 2448.72 370.26 3.06 918.6500 0.19 40.0900 276.73 521574 CO2Monitor_Ctrl_Exp1% 2012-11-19T16:00 0 Pleurochrysis cf. pseudoroscoffensis 17047 9.14 10.02 8.5 CO2Monitor_Ctrl_Exp1% 2012-11-20T08:00 0 Pleurochrysis cf. pseudoroscoffensis 8.4 CO2Monitor_Ctrl_Exp1% 2012-11-20T16:00 1 Pleurochrysis cf. pseudoroscoffensis 31617 8.5 0.90 776.3300 2.60 19.4700 269.67 CO2Monitor_Ctrl_Exp1% 2012-11-21T08:00 1 Pleurochrysis cf. pseudoroscoffensis 8.5 CO2Monitor_Ctrl_Exp1% 2012-11-21T17:00 2 Pleurochrysis cf. pseudoroscoffensis 95800 8.7 195.86 1800.26 277.24 0.52 654.8100 4.31 5.0500 266.74 2039172 CO2Monitor_Ctrl_Exp1% 2012-11-22T08:00 2 Pleurochrysis cf. pseudoroscoffensis 129532 8.5 214.70 2027.24 465.94 CO2Monitor_Ctrl_Exp1% 2012-11-22T17:00 3 Pleurochrysis cf. pseudoroscoffensis 157891 2.23 1.47 7.53 8.17 8.7 419.21 3483.55 691.84 0.58 580.2100 5.19 0.6931 267.38 CO2Monitor_Ctrl_Exp1% 2012-11-23T08:00 3 Pleurochrysis cf. pseudoroscoffensis 2.49 1.68 8.3 CO2Monitor_Ctrl_Exp1% 2012-11-23T17:00 4 Pleurochrysis cf. pseudoroscoffensis 180106 8.7 396.17 3342.67 1100.38 0.73 456.6200 6.20 0.7300 268.13 1010033 CO2Monitor_Ctrl_Exp1% 2012-11-24T08:00 4 Pleurochrysis cf. pseudoroscoffensis 199370 8.0 CO2Monitor_Ctrl_Exp1% 2012-11-24T17:00 5 Pleurochrysis cf. pseudoroscoffensis 208737 8.7 0.90 320.0800 6.83 0.9500 282.40 CO2Monitor_Ctrl_Exp1% 2012-11-25T08:00 5 Pleurochrysis cf. pseudoroscoffensis CO2Monitor_Ctrl_Exp1% 2012-11-25T17:00 6 Pleurochrysis cf. pseudoroscoffensis 258818 8.7 590.63 5228.01 941.62 0.52 169.6600 7.17 0.6900 275.60 2808057 CO2Monitor_Ctrl_Exp1% 2012-11-26T08:00 6 Pleurochrysis cf. pseudoroscoffensis 249070 7.9 587.80 5096.12 1097.11 CO2Monitor_Ctrl_Exp1% 2012-11-26T17:00 7 Pleurochrysis cf. pseudoroscoffensis 257671 8.8 612.55 5710.85 752.06 0.54 61.6650 7.28 0.6400 289.30 CO2Monitor_Ctrl_Exp1% 2012-11-27T08:00 7 Pleurochrysis cf. pseudoroscoffensis 7.9 528.93 6289.55 CO2Monitor_Ctrl_Exp1% 2012-11-27T17:00 8 Pleurochrysis cf. pseudoroscoffensis 286153 7.68 8.41 8.6 0.58 0.1084 0.13 0.6300 278.47 CO2Monitor_Ctrl_Exp1% 2012-11-28T08:00 8 Pleurochrysis cf. pseudoroscoffensis 7.8 CO2Monitor_Ctrl_Exp1% 2012-11-28T17:00 9 Pleurochrysis cf. pseudoroscoffensis 276022 2.13 1.33 8.47 8.86 8.5 620.23 6490.07 1713.75 0.68 0.0030 0.10 0.6404 295.37 1086040 CO2Monitor_Ctrl_Exp1% 2012-11-29T08:00 9 Pleurochrysis cf. pseudoroscoffensis 240086 2.28 1.45 7.8 497.16 6412.82 957.70 CO2Monitor_CO2_Exp1% 2012-11-19T12:00 0 Pleurochrysis cf. pseudoroscoffensis 20000 7.17 7.73 7.6 283.10 2448.72 370.26 3.06 918.6500 0.19 40.0900 276.73 330669 CO2Monitor_CO2_Exp1% 2012-11-19T16:00 0 Pleurochrysis cf. pseudoroscoffensis 13856 8.73 9.59 7.7 CO2Monitor_CO2_Exp1% 2012-11-20T08:00 0 Pleurochrysis cf. pseudoroscoffensis 7.9 CO2Monitor_CO2_Exp1% 2012-11-20T16:00 1 Pleurochrysis cf. pseudoroscoffensis 44825 7.6 0.61 778.3650 2.54 16.4450 271.99 CO2Monitor_CO2_Exp1% 2012-11-21T08:00 1 Pleurochrysis cf. pseudoroscoffensis 7.7 CO2Monitor_CO2_Exp1% 2012-11-21T17:00 2 Pleurochrysis cf. pseudoroscoffensis 104151 7.8 307.95 2389.43 548.71 1.26 571.6450 4.86 1.4564 269.60 586133 CO2Monitor_CO2_Exp1% 2012-11-22T08:00 2 Pleurochrysis cf. pseudoroscoffensis 172318 7.5 434.07 2678.51 1411.48 CO2Monitor_CO2_Exp1% 2012-11-22T17:00 3 Pleurochrysis cf. pseudoroscoffensis 164906 2.37 1.55 7.31 8.10 7.6 0.71 331.7250 6.26 0.8381 278.48 CO2Monitor_CO2_Exp1% 2012-11-23T08:00 3 Pleurochrysis cf. pseudoroscoffensis 2.27 1.40 7.4 CO2Monitor_CO2_Exp1% 2012-11-23T17:00 4 Pleurochrysis cf. pseudoroscoffensis 309655 7.6 827.08 6722.30 1313.11 1.31 78.0029 1.05 0.9900 294.45 515392 CO2Monitor_CO2_Exp1% 2012-11-24T08:00 4 Pleurochrysis cf. pseudoroscoffensis 263788 7.3 620.85 6774.56 1401.25 CO2Monitor_CO2_Exp1% 2012-11-24T17:00 5 Pleurochrysis cf. pseudoroscoffensis 381155 7.6 0.68 1.8504 0.07 0.7000 295.39 CO2Monitor_CO2_Exp1% 2012-11-25T08:00 5 Pleurochrysis cf. pseudoroscoffensis CO2Monitor_CO2_Exp1% 2012-11-25T17:00 6 Pleurochrysis cf. pseudoroscoffensis 432383 7.5 728.14 9503.96 2938.41 1.09 6.2944 0.46 1.1200 293.89 2609938 CO2Monitor_CO2_Exp1% 2012-11-26T08:00 6 Pleurochrysis cf. pseudoroscoffensis 380199 7.1 705.38 8887.12 3282.94 CO2Monitor_CO2_Exp1% 2012-11-26T17:00 7 Pleurochrysis cf. pseudoroscoffensis 397403 7.4 599.15 10753.60 2353.84 1.18 0.7470 0.18 1.2300 293.43 CO2Monitor_CO2_Exp1% 2012-11-27T08:00 7 Pleurochrysis cf. pseudoroscoffensis 7.2 964.63 11866.09 2674.61 CO2Monitor_CO2_Exp1% 2012-11-27T17:00 8 Pleurochrysis cf. pseudoroscoffensis 397403 8.89 9.40 7.5 1.93 0.0389 0.15 1.3200 321.25 CO2Monitor_CO2_Exp1% 2012-11-28T08:00 8 Pleurochrysis cf. pseudoroscoffensis 7.1 CO2Monitor_CO2_Exp1% 2012-11-28T17:00 9 Pleurochrysis cf. pseudoroscoffensis 322854 2.18 1.33 9.33 9.77 7.7 576.33 12173.62 1875.31 3.32 0.0254 0.13 2.0250 287.92 434769 CO2Monitor_CO2_Exp1% 2012-11-29T08:00 9 Pleurochrysis cf. pseudoroscoffensis 341395 2.14 1.34 7.2 622.16 12828.85 1516.57 CO2Monitor_Ctrl_Exp2% 2013-06-10T11:00 0 Pleurochrysis cf. pseudoroscoffensis 20000 6.69 7.51 8.2 3.55 904.5700 0.08 36.0600 262.16 474489 CO2Monitor_Ctrl_Exp2% 2013-06-10T15:00 0 Pleurochrysis cf. pseudoroscoffensis 15195 9.29 9.96 8.3 47.35 1309.86 CO2Monitor_Ctrl_Exp2% 2013-06-11T07:00 0 Pleurochrysis cf. pseudoroscoffensis 21175 8.0 74.37 1027.55 380.28 CO2Monitor_Ctrl_Exp2% 2013-06-11T15:00 1 Pleurochrysis cf. pseudoroscoffensis 35388 8.3 1.70 837.3600 4.33 19.5400 248.97 CO2Monitor_Ctrl_Exp2% 2013-06-12T07:00 1 Pleurochrysis cf. pseudoroscoffensis 48237 7.9 CO2Monitor_Ctrl_Exp2% 2013-06-12T15:00 2 Pleurochrysis cf. pseudoroscoffensis 78395 2.28 1.43 8.4 234.15 1969.96 98.16 3.69 695.0800 6.54 7.9000 248.72 1539763 CO2Monitor_Ctrl_Exp2% 2013-06-13T07:00 2 Pleurochrysis cf. pseudoroscoffensis 126398 2.30 1.45 7.8 256.29 1276.65 1553.88 CO2Monitor_Ctrl_Exp2% 2013-06-13T15:00 3 Pleurochrysis cf. pseudoroscoffensis 165375 7.20 7.73 8.6 5.53 565.9000 7.54 0.6900 252.97 CO2Monitor_Ctrl_Exp2% 2013-06-14T07:00 3 Pleurochrysis cf. pseudoroscoffensis 178558 7.7 CO2Monitor_Ctrl_Exp2% 2013-06-14T15:00 4 Pleurochrysis cf. pseudoroscoffensis 253657 8.7 419.46 3829.64 612.95 1.39 396.6100 8.49 0.7800 253.77 2240642 CO2Monitor_Ctrl_Exp2% 2013-06-15T07:00 4 Pleurochrysis cf. pseudoroscoffensis 222691 7.7 582.97 4623.72 1005.93 CO2Monitor_Ctrl_Exp2% 2013-06-15T15:00 5 Pleurochrysis cf. pseudoroscoffensis 254231 8.6 13.56 228.0400 8.16 1.2600 272.36 CO2Monitor_Ctrl_Exp2% 2013-06-16T07:00 5 Pleurochrysis cf. pseudoroscoffensis 250599 7.7 CO2Monitor_Ctrl_Exp2% 2013-06-16T15:00 6 Pleurochrysis cf. pseudoroscoffensis 243717 2.23 1.40 8.6 788.77 6583.33 1289.82 1.25 55.3800 9.10 0.6400 259.95 2726842 CO2Monitor_Ctrl_Exp2% 2013-06-17T07:00 6 Pleurochrysis cf. pseudoroscoffensis 271052 2.19 1.37 7.7 755.10 6880.71 1021.69 CO2Monitor_Ctrl_Exp2% 2013-06-17T15:00 7 Pleurochrysis cf. pseudoroscoffensis 268567 8.02 8.49 8.5 531.58 5646.75 1099.47 5.57 12.3100 0.10 1.5400 265.34 5670771 CO2Monitor_Ctrl_Exp2% 2013-06-19T07:00 7 Pleurochrysis cf. pseudoroscoffensis 256907 7.6 CO2Monitor_CO2_Exp2% 2013-06-10T11:00 0 Pleurochrysis cf. pseudoroscoffensis 20000 6.69 7.51 7.0 3.55 904.5700 0.08 36.0600 262.16 334933 CO2Monitor_CO2_Exp2% 2013-06-10T15:00 0 Pleurochrysis cf. pseudoroscoffensis 15329 9.29 9.96 7.2 49.19 489.76 30.61 CO2Monitor_CO2_Exp2% 2013-06-11T07:00 0 Pleurochrysis cf. pseudoroscoffensis 20132 7.1 76.58 888.29 10.45 CO2Monitor_CO2_Exp2% 2013-06-11T15:00 1 Pleurochrysis cf. pseudoroscoffensis 30987 7.1 1.44 833.8800 3.47 19.8800 245.01 CO2Monitor_CO2_Exp2% 2013-06-12T07:00 1 Pleurochrysis cf. pseudoroscoffensis 64451 7.1 CO2Monitor_CO2_Exp2% 2013-06-12T15:00 2 Pleurochrysis cf. pseudoroscoffensis 126933 2.30 1.47 7.1 267.94 2023.23 423.59 1.51 646.0200 5.48 6.3900 252.46 509378 CO2Monitor_CO2_Exp2% 2013-06-13T07:00 2 Pleurochrysis cf. pseudoroscoffensis 204971 7.2 390.41 2908.24 825.28 CO2Monitor_CO2_Exp2% 2013-06-13T15:00 3 Pleurochrysis cf. pseudoroscoffensis 215836 7.20 7.73 7.1 9.76 376.9200 7.22 0.6600 255.42 CO2Monitor_CO2_Exp2% 2013-06-14T07:00 3 Pleurochrysis cf. pseudoroscoffensis 201665 7.2 CO2Monitor_CO2_Exp2% 2013-06-14T15:00 4 Pleurochrysis cf. pseudoroscoffensis 315399 7.2 872.28 5736.36 1683.63 15.21 72.9500 0.06 1.8100 252.77 2249307 CO2Monitor_CO2_Exp2% 2013-06-15T07:00 4 Pleurochrysis cf. pseudoroscoffensis 262832 7.2 738.10 8219.36 1377.07 CO2Monitor_CO2_Exp2% 2013-06-15T15:00 5 Pleurochrysis cf. pseudoroscoffensis 382302 6.9 4.64 45.6400 0.19 1.6800 277.76 CO2Monitor_CO2_Exp2% 2013-06-16T07:00 5 Pleurochrysis cf. pseudoroscoffensis 277169 6.9 CO2Monitor_CO2_Exp2% 2013-06-16T15:00 6 Pleurochrysis cf. pseudoroscoffensis 385360 2.22 1.40 6.9 872.62 13102.32 3096.13 3.84 29.2800 0.14 1.7700 281.18 6217200 CO2Monitor_CO2_Exp2% 2013-06-17T07:00 6 Pleurochrysis cf. pseudoroscoffensis 292799 2.23 1.40 7.2 619.09 12618.56 820.41 CO2Monitor_CO2_Exp2% 2013-06-17T15:00 7 Pleurochrysis cf. pseudoroscoffensis 187744 8.02 8.49 7.1 461.77 10820.44 238.13 13.91 48.7900 0.28 1.8800 278.85 6656800 CO2Monitor_CO2_Exp2% 2013-06-19T07:00 7 Pleurochrysis cf. pseudoroscoffensis 268949 7.2