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Spungin, D; Berman-Frank, I; Levitan, Orly (2014): Trichodesmium's strategies to alleviate phosphorus limitation in the future acidified oceans [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.833817, Supplement to: Spungin, D et al. (2014): Trichodesmium's strategies to alleviate phosphorus limitation in the future acidified oceans. Environmental Microbiology, 16(6), 1935-1947, https://doi.org/10.1111/1462-2920.12424

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Abstract:
Global warming may exacerbate inorganic nutrient limitation, including phosphorus (P), in the surface-waters of tropical oceans that are home to extensive blooms of the marine diazotrophic cyanobacterium, Trichodesmium. We examined the combined effects of P limitation and pCO2, forecast under ocean acidification scenarios, on Trichodesmium erythraeum IMS101 cultures. We measured nitrogen acquisition, glutamine synthetase activity, C uptake rates, intracellular Adenosine Triphosphate (ATP) concentration and the pool sizes of related key proteins. Here, we present data supporting the idea that cellular energy re-allocation enables the higher growth and N2 fixation rates detected in Trichodesmium cultured under high pCO2. This is reflected in altered protein abundance and metabolic pools. Also modified are particulate organic carbon and nitrogen production rates, enzymatic activities, and cellular ATP concentrations. We suggest that adjusting these cellular pathways to changing environmental conditions enables Trichodesmium to compensate for low P availability and to thrive in acidified oceans. Moreover, elevated pCO2 could provide Trichodesmium with a competitive dominance that would extend its niche, particularly in P-limited regions of the tropical and subtropical oceans.
Keyword(s):
Bacteria; Bottles or small containers/Aquaria (<20 L); Cyanobacteria; Laboratory experiment; Laboratory strains; Macro-nutrients; Not applicable; Other metabolic rates; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Trichodesmium erythraeum
Further details:
Lavigne, Héloïse; Epitalon, Jean-Marie; Gattuso, Jean-Pierre (2014): seacarb: seawater carbonate chemistry with R. R package version 3.0. https://cran.r-project.org/package=seacarb
Project(s):
Ocean Acidification International Coordination Centre (OA-ICC)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-07-07.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1SpeciesSpeciesLevitan, Orly
2Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmLevitan, Orly
3Phosphate[PO4]3-µmol/lLevitan, Orly
4Incubation durationInc durhLevitan, Orly
5Glutamine synthetase biosynthetic activity per cellGS biosynth act/cellµmol/#/hLevitan, Orly
6Glutamine synthetase biosynthetic activity, standard deviationGS biosynth act std dev±Levitan, Orly
7Carbon uptake rate per cellC upt rate/cellmg/#/hLevitan, Orly
8Carbon uptake rate, standard deviationC upt rate std dev±Levitan, Orly
9Adenosine 5-Triphosphate, per cellATP/cellfmol/#Levitan, Orly
10Adenosine 5-Triphosphate, standard deviationATP std dev±Levitan, Orly
11Glutamine synthetase transferase/biosynthetic activity ratioGST/GS biosynth actLevitan, Orly
12Glutamine synthetase transferase/biosynthetic activity ratio, standard deviationGST/GS biosynth act std dev±Levitan, Orly
13Carbon dioxideCO2µmol/kgLevitan, OrlyCalculated using CO2SYS
14Carbon dioxide, standard deviationCO2 std dev±Levitan, OrlyCalculated using CO2SYS
15pHpHLevitan, OrlyNBS scale
16pH, standard deviationpH std dev±Levitan, OrlyNBS scale
17Carbon, inorganic, dissolvedDICµmol/kgLevitan, OrlyCalculated using CO2SYS
18Carbon, inorganic, dissolved, standard deviationDIC std dev±Levitan, OrlyCalculated using CO2SYS
19Alkalinity, totalATµmol/kgLevitan, Orly
20Alkalinity, total, standard deviationAT std dev±Levitan, Orly
21SalinitySalLevitan, Orly
22Temperature, waterTemp°CLevitan, Orly
23Growth rateµ#/dayLevitan, Orly
24Growth rate, standard deviationµ std dev±Levitan, Orly
25LengthlmmLevitan, Orlyfilament
26Length, standard deviationl std dev±Levitan, Orlyfilament
27LengthlµmLevitan, Orlycell
28Length, standard deviationl std dev±Levitan, Orlycell
29Chlorophyll a per cellChl a/cellpg/#Levitan, Orly
30Chlorophyll a, standard deviationChl a std dev±Levitan, Orly
31Carbon/Nitrogen ratioC/NLevitan, Orly
32Carbon/Nitrogen ratio, standard deviationC/N std dev±Levitan, Orly
33Carbon/Phosphorus ratioC/PLevitan, Orly
34Carbon/Phosphorus ratio, standard deviationC/P std dev±Levitan, Orly
35Nitrogen/Phosphorus ratioN/PLevitan, Orly
36Nitrogen/Phosphorus ratio, standard deviationN/P std dev±Levitan, Orly
37Particulate organic phosphorus per cellPOP/cellpmol/#Levitan, Orly
38Particulate organic phosphorus, standard deviationPOP std dev±Levitan, Orly
39Particulate organic nitrogen per cellPON/cellpmol/#Levitan, Orly
40Particulate organic nitrogen, standard deviationPON std dev±Levitan, Orly
41Particulate organic carbon, per cellPOC/cellpmol/#Levitan, Orly
42Particulate organic carbon content per cell, standard deviationPOC cont/cell std dev±Levitan, Orly
43Production of particulate organic nitrogenPON prodpg/#/dayLevitan, Orly
44Particulate organic nitrogen production, standard deviationPON prod std dev±Levitan, Orly
45Particulate organic carbon production per cellPOC prod/cellpg/#/dayLevitan, Orly
46Particulate organic carbon, production, standard deviationPOC prod std dev±Levitan, Orly
47Photosynthetic protein, PsbCPsaCpmol/µgLevitan, Orly
48Photosynthetic protein, PsbC, standard deviationPsaC std dev±Levitan, Orly
49Photosynthetic protein PsbAPsbApmol/µgLevitan, Orly
50Photosynthetic protein, PsbA, standard deviationPsbA std dev±Levitan, Orly
51Photosynthetic protein RubiscoRbcLpmol/mgLevitan, Orly
52Photosynthetic protein Rubisco, standard deviationRbcL std dev±Levitan, Orly
53CF1 subunit of ATP synthase proteinAtpBpmol/mgLevitan, Orly
54CF1 subunit of ATP synthase protein, standard deviationAtpB std dev±Levitan, Orly
55GlnA subunit of Gln synthetaseGlnApmol/mgLevitan, Orly
56GlnA subunit of Gln synthetase, standard deviationGlnA std dev±Levitan, Orly
57Iron protein of nitrogenaseNifHpmol/mgLevitan, Orly
58Iron protein of nitrogenase, standard deviationNifH std dev±Levitan, Orly
59Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
60pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
61Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
62Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
63Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
64Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
65Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
66Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
67Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
68Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1003 data points

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