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Venn, Alexander A; Tambutté, Eric; Holcomb, Michael; Laurent, Julien; Allemand, Denis; Tambutté, Sylvie (2013): Seawater carbonate chemistry, tissue-skeleton interface and calcification in reef corals in a laboratory experiment [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.830817, Supplement to: Venn, AA et al. (2012): Impact of seawater acidification on pH at the tissue-skeleton interface and calcification in reef corals. Proceedings of the National Academy of Sciences, 110(5), 1634-1639, https://doi.org/10.1073/pnas.1216153110

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Published: 2013 (exact date unknown) • DOI registered: 2014-05-30

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Abstract:
Insight into the response of reef corals and other major marine calcifiers to ocean acidification is limited by a lack of knowledge about how seawater pH and carbonate chemistry impact the physiological processes that drive biomineralization. Ocean acidification is proposed to reduce calcification rates in corals by causing declines in internal pH at the calcifying tissue-skeleton interface where biomineralization takes place. Here, we performed an in vivo study on how partial-pressure CO(2)-driven seawater acidification impacts intracellular pH in coral calcifying cells and extracellular pH in the fluid at the tissue-skeleton interface [subcalicoblastic medium (SCM)] in the coral Stylophora pistillata. We also measured calcification in corals grown under the same conditions of seawater acidification by measuring lateral growth of colonies and growth of aragonite crystals under the calcifying tissue. Our findings confirm that seawater acidification decreases pH of the SCM, but this decrease is gradual relative to the surrounding seawater, leading to an increasing pH gradient between the SCM and seawater. Reductions in calcification rate, both at the level of crystals and whole colonies, were only observed in our lowest pH treatment when pH was significantly depressed in the calcifying cells in addition to the SCM. Overall, our findings suggest that reef corals may mitigate the effects of seawater acidification by regulating pH in the SCM, but they also highlight the role of calcifying cell pH homeostasis in determining the response of reef corals to changes in external seawater pH and carbonate chemistry.
Keyword(s):
Calcification/Dissolution; Laboratory experiment; Laboratory strains; Micro-nutrients; Not applicable; Stylophora pistillata
Further details:
Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4 [webpage]. https://cran.r-project.org/package=seacarb
Project(s):
Ocean Acidification International Coordination Centre (OA-ICC)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-03-21.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1SpeciesSpeciesVenn, Alexander A
2IdentificationIDVenn, Alexander A
3ReplicateReplVenn, Alexander A
4TreatmentTreatVenn, Alexander A
5Surface areaSAcm2Venn, Alexander Aincraese in lateral surface area of whole colonies growth
6ChangeChange%Venn, Alexander Amean change in cross sectional area of aragonite crystals (% of original size of crystal)
7LocationLocationVenn, Alexander A
8GroupGroupVenn, Alexander A
9pH, NBS scalepH NBSVenn, Alexander ANBS scale, shown in table 2
10pH, standard deviationpH std dev±Venn, Alexander ANBS scale, shown in table 2
11Temperature, waterTemp°CVenn, Alexander A
12SalinitySalVenn, Alexander A
13pH, NBS scalepH NBSVenn, Alexander ANBS scale
14pH, standard deviationpH std dev±Venn, Alexander ANBS scale
15pH, total scalepHTVenn, Alexander Atotal scale
16pH, standard deviationpH std dev±Venn, Alexander Atotal scale
17Alkalinity, totalATµmol/kgVenn, Alexander APotentiometric titration
18Alkalinity, total, standard deviationAT std dev±Venn, Alexander APotentiometric titration
19Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmVenn, Alexander ACalculated using CO2SYS
20Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Venn, Alexander ACalculated using CO2SYS
21Bicarbonate ion[HCO3]-µmol/kgVenn, Alexander ACalculated using CO2SYS
22Bicarbonate ion, standard deviation[HCO3]- std dev±Venn, Alexander ACalculated using CO2SYS
23Carbonate ion[CO3]2-µmol/kgVenn, Alexander ACalculated using CO2SYS
24Carbonate ion, standard deviation[CO3]2- std dev±Venn, Alexander ACalculated using CO2SYS
25Carbon, inorganic, dissolvedDICµmol/kgVenn, Alexander ACalculated using CO2SYS
26Carbon, inorganic, dissolved, standard deviationDIC std dev±Venn, Alexander ACalculated using CO2SYS
27Aragonite saturation stateOmega ArgVenn, Alexander ACalculated using CO2SYS
28Aragonite saturation state, standard deviationOmega Arg std dev±Venn, Alexander ACalculated using CO2SYS
29Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
30Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
31Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
32Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
33Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
34Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
35Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
36Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
37Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
2880 data points

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