Data Description

Citation:
Bach, Lennart Thomas; Mackinder, Luke C M; Yang, Yan (2014): Experiment: Dissecting the impact of CO2 and pH on the mechanisms of photosynthesis and calcification in the coccolithophore Emiliania huxleyi. doi:10.1594/PANGAEA.830627,
Supplement to: Bach, Lennart Thomas; Mackinder, Luke C M; Schulz, Kai Georg; Wheeler, Glen; Schroeder, Declan C; Brownlee, Colin; Riebesell, Ulf (2013): Dissecting the impact of CO2 and pH on the mechanisms of photosynthesis and calcification in the coccolithophore Emiliania huxleyi. New Phytologist, 199(1), 121-134, doi:10.1111/nph.12225
Abstract:
Coccolithophores are important calcifying phytoplankton predicted to be impacted by changes in ocean carbonate chemistry caused by the absorption of anthropogenic CO2. However, it is difficult to disentangle the effects of the simultaneously changing carbonate system parameters (CO2, bicarbonate, carbonate and protons) on the physiological responses to elevated CO2. Here, we adopted a multifactorial approach at constant pH or CO2 whilst varying dissolved inorganic carbon (DIC) to determine physiological and transcriptional responses to individual carbonate system parameters. We show that Emiliania huxleyi is sensitive to low CO2 (growth and photosynthesis) and low bicarbonate (calcification) as well as low pH beyond a limited tolerance range, but is much less sensitive to elevated CO2 and bicarbonate. Multiple up-regulated genes at low DIC bear the hallmarks of a carbon-concentrating mechanism (CCM) that is responsive to CO2 and bicarbonate but not to pH. Emiliania huxleyi appears to have evolved mechanisms to respond to limiting rather than elevated CO2. Calcification does not function as a CCM, but is inhibited at low DIC to allow the redistribution of DIC from calcification to photosynthesis. The presented data provides a significant step in understanding how E. huxleyi will respond to changing carbonate chemistry at a cellular level
Further details:
Lavigne, Héloise; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb *
Project(s):
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-02-11.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Species *SpeciesBach, Lennart Thomas *
2Treatment *TreatmBach, Lennart Thomas *
3Treatment *TreatmBach, Lennart Thomas *
4Irradiance *EµE/m2/sBach, Lennart Thomas *
5Light:Dark cycle *L:Dhh:hhBach, Lennart Thomas *
6Temperature, water *Temp°CBach, Lennart Thomas *
7Salinity *SalBach, Lennart Thomas *
8Carbon, inorganic, dissolved *DICµmol/kgBach, Lennart Thomas *
9Difference *DiffBach, Lennart Thomas *DIC difference from t0 to tfin
10pH *pHBach, Lennart Thomas *free scale
11Difference *DiffBach, Lennart Thomas *pH difference from t0 to tfin
12Bicarbonate ion *[HCO3]-µmol/kgBach, Lennart Thomas *
13Difference *DiffBach, Lennart Thomas *HCO3 difference from t0 to tfin
14Carbonate ion *[CO3]2-µmol/kgBach, Lennart Thomas *
15Difference *DiffBach, Lennart Thomas *CO32 difference from t0 to tfin
16Carbon dioxide *CO2µmol/kgBach, Lennart Thomas *
17Difference *DiffBach, Lennart Thomas *CO2 difference from t0 to tfin
18Growth rate *µ1/dayBach, Lennart Thomas *
19Difference *DiffBach, Lennart Thomas *growth rate difference from t0 to tfin
20Particulate inorganic carbon/particulate organic carbon ratio *PIC/POC ratioBach, Lennart Thomas *
21Difference *DiffBach, Lennart Thomas *PIC/POC difference from t0 to tfin
22Chlorophyll a *Chl aµg/lBach, Lennart Thomas *
23Difference *DiffBach, Lennart Thomas *chl a difference from t0 to tfin
24Production of particulate organic carbon per cell *POC prodpg/#/dayBach, Lennart Thomas *
25Difference *DiffBach, Lennart Thomas *POC proddifference from t0 to tfin
26Particulate inorganic carbon production per cell *PIC prodpg/#/dayBach, Lennart Thomas *
27Difference *DiffBach, Lennart Thomas *PIC difference from t0 to tfin
28Carbon/Nitrogen ratio *C/NBach, Lennart Thomas *
29Difference *DiffBach, Lennart Thomas *C/N difference from t0 to tfin
30Anion exchanger like 1 *AEL1Bach, Lennart Thomas *
31Anion exchanger like 1, standard error *AEL1 std e±Bach, Lennart Thomas *
32alpha carbonic anhydrase 1 *alpha CA1Bach, Lennart Thomas *
33alpha carbonic anhydrase 1, standard error *alpha CA1 std e±Bach, Lennart Thomas *
34alpha carbonic anhydrase 2 *alpha CA2Bach, Lennart Thomas *
35alpha carbonic anhydrase 2, standard error *alpha CA2 std e±Bach, Lennart Thomas *
36beta carbonic anhydrase mean *beta CABach, Lennart Thomas *
37beta carbonic anhydrase, standard error *beta CA std e±Bach, Lennart Thomas *
38gamma carbonic anhydrase, mean *gamma CABach, Lennart Thomas *
39gamma carbonic anhydrase, standard error *gamma CA std e±Bach, Lennart Thomas *
40delta carbonic anhydrase, mean *delta CABach, Lennart Thomas *
41delta carbonic anhydrase, standard error *delta CA std e±Bach, Lennart Thomas *
42RubisCO *RubisCOBach, Lennart Thomas *
43RubisCO, standard error *RubisCO std e±Bach, Lennart Thomas *
44Voltage-gated H+ channel *HVCN1Bach, Lennart Thomas *
45Voltage-gated H+ channel, standard error *HVCN1 std e±Bach, Lennart Thomas *
46Vacuolar-type H+ pump *ATPVc_/cBach, Lennart Thomas *
47Vacuolar-type H+ pump, standard error *ATPVc_/c std e±Bach, Lennart Thomas *
48Plasma membran type H+ pump *PATPBach, Lennart Thomas *
49Plasma membran type H+ pump, standard error *PATP std e±Bach, Lennart Thomas *
50Ca2+/H+ exchanger3 *CAX3Bach, Lennart Thomas *
51Ca2+/H+ exchanger 3, standard error *CAX3 std e±Bach, Lennart Thomas *
52Na+/H+ exchanger 2 *NhaA2Bach, Lennart Thomas *
53Na+/H+ exchanger 2, standard error *NhaA2 std e±Bach, Lennart Thomas *
54Low CO2 induced gene *LCIXBach, Lennart Thomas *
55Low CO2 induced gene, standard error *LCIX std e±Bach, Lennart Thomas *
56Aquaporin 2 *AQP2Bach, Lennart Thomas *
57Aquaporin 2, standard error *AQP2 std e±Bach, Lennart Thomas *
58Glutamic acid, proline, alanine rich protein *GPABach, Lennart Thomas *
59Glutamic acid, proline, alanine rich protein, standard error *GPA std e±Bach, Lennart Thomas *
60Carbonate system computation flag *CSC flagYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
61pH *pHYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *total scale
62Carbon dioxide *CO2µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
63Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
64Fugacity of carbon dioxide (water) at sea surface temperature (wet air) *fCO2water_SST_wetµatmYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
65Bicarbonate ion *[HCO3]-µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
66Carbonate ion *[CO3]2-µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
67Alkalinity, total *ATµmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
68Aragonite saturation state *Omega ArgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
69Calcite saturation state *Omega CalYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
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Size:
1165 data points

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