Citation:

nph.12225

Name: Experiment: Dissecting the impact of CO2 and pH on the mechanisms of photosynthesis and calcification in the coccolithophore Emiliania huxleyi
Published: 2013
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Calcification/Dissolution; Chromista; Containers and aquaria (20-1000 L or < 1 m**2); Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species

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Abstract:

Coccolithophores are important calcifying phytoplankton predicted to be impacted by changes in ocean carbonate chemistry caused by the absorption of anthropogenic CO2. However, it is difficult to disentangle the effects of the simultaneously changing carbonate system parameters (CO2, bicarbonate, carbonate and protons) on the physiological responses to elevated CO2. Here, we adopted a multifactorial approach at constant pH or CO2 whilst varying dissolved inorganic carbon (DIC) to determine physiological and transcriptional responses to individual carbonate system parameters. We show that Emiliania huxleyi is sensitive to low CO2 (growth and photosynthesis) and low bicarbonate (calcification) as well as low pH beyond a limited tolerance range, but is much less sensitive to elevated CO2 and bicarbonate. Multiple up-regulated genes at low DIC bear the hallmarks of a carbon-concentrating mechanism (CCM) that is responsive to CO2 and bicarbonate but not to pH. Emiliania huxleyi appears to have evolved mechanisms to respond to limiting rather than elevated CO2. Calcification does not function as a CCM, but is inhibited at low DIC to allow the redistribution of DIC from calcification to photosynthesis. The presented data provides a significant step in understanding how E. huxleyi will respond to changing carbonate chemistry at a cellular level

Keyword(s):

Calcification/Dissolution; Chromista; Containers and aquaria (20-1000 L or < 1 m**2); Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species

Further details:

Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb

Project(s):

Biological Impacts of Ocean Acidification (BIOACID)

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-02-11.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Bach, Lennart Thomas
2	Treatment	Treat		Bach, Lennart Thomas
3	Treatment	Treat		Bach, Lennart Thomas
4	Irradiance	E	µmol/m²/s	Bach, Lennart Thomas
5	Light:Dark cycle	L:D	hh:hh	Bach, Lennart Thomas
6	Temperature, water	Temp	°C	Bach, Lennart Thomas
7	Salinity	Sal		Bach, Lennart Thomas
8	Carbon, inorganic, dissolved	DIC	µmol/kg	Bach, Lennart Thomas
9	Difference	Diff		Bach, Lennart Thomas		DIC difference from t0 to tfin
10	pH	pH		Bach, Lennart Thomas		free scale
11	Difference	Diff		Bach, Lennart Thomas		pH difference from t0 to tfin
12	Bicarbonate ion	[HCO3]-	µmol/kg	Bach, Lennart Thomas
13	Difference	Diff		Bach, Lennart Thomas		HCO3 difference from t0 to tfin
14	Carbonate ion	[CO3]2-	µmol/kg	Bach, Lennart Thomas
15	Difference	Diff		Bach, Lennart Thomas		CO32 difference from t0 to tfin
16	Carbon dioxide	CO2	µmol/kg	Bach, Lennart Thomas
17	Difference	Diff		Bach, Lennart Thomas		CO2 difference from t0 to tfin
18	Growth rate	µ	1/day	Bach, Lennart Thomas
19	Difference	Diff		Bach, Lennart Thomas		growth rate difference from t0 to tfin
20	Particulate inorganic carbon/particulate organic carbon ratio	PIC/POC		Bach, Lennart Thomas
21	Difference	Diff		Bach, Lennart Thomas		PIC/POC difference from t0 to tfin
22	Chlorophyll a	Chl a	µg/l	Bach, Lennart Thomas
23	Difference	Diff		Bach, Lennart Thomas		chl a difference from t0 to tfin
24	Particulate organic carbon production per cell	POC prod/cell	pg/#/day	Bach, Lennart Thomas
25	Difference	Diff		Bach, Lennart Thomas		POC proddifference from t0 to tfin
26	Particulate inorganic carbon production per cell	PIC prod/cell	pg/#/day	Bach, Lennart Thomas
27	Difference	Diff		Bach, Lennart Thomas		PIC difference from t0 to tfin
28	Carbon/Nitrogen ratio	C/N		Bach, Lennart Thomas
29	Difference	Diff		Bach, Lennart Thomas		C/N difference from t0 to tfin
30	Anion exchanger like 1	AEL1		Bach, Lennart Thomas
31	Anion exchanger like 1, standard error	AEL1 std e	±	Bach, Lennart Thomas
32	alpha carbonic anhydrase 1	alpha CA1		Bach, Lennart Thomas
33	alpha carbonic anhydrase 1, standard error	alpha CA1 std e	±	Bach, Lennart Thomas
34	alpha carbonic anhydrase 2	alpha CA2		Bach, Lennart Thomas
35	alpha carbonic anhydrase 2, standard error	alpha CA2 std e	±	Bach, Lennart Thomas
36	beta carbonic anhydrase	beta CA		Bach, Lennart Thomas
37	beta carbonic anhydrase, standard error	beta CA std e	±	Bach, Lennart Thomas
38	gamma carbonic anhydrase, mean	gamma CA		Bach, Lennart Thomas
39	gamma carbonic anhydrase, standard error	gamma CA std e	±	Bach, Lennart Thomas
40	delta carbonic anhydrase	delta CA		Bach, Lennart Thomas
41	delta carbonic anhydrase, standard error	delta CA std e	±	Bach, Lennart Thomas
42	RubisCO	RubisCO		Bach, Lennart Thomas
43	RubisCO, standard error	RubisCO std e	±	Bach, Lennart Thomas
44	Voltage-gated H+ channel	HVCN1		Bach, Lennart Thomas
45	Voltage-gated H+ channel, standard error	HVCN1 std e	±	Bach, Lennart Thomas
46	Vacuolar-type H+ pump	ATPVc_/c		Bach, Lennart Thomas
47	Vacuolar-type H+ pump, standard error	ATPVc_/c std e	±	Bach, Lennart Thomas
48	Plasma membran type H+ pump	PATP		Bach, Lennart Thomas
49	Plasma membran type H+ pump, standard error	PATP std e	±	Bach, Lennart Thomas
50	Ca2+/H+ exchanger 3	CAX3		Bach, Lennart Thomas
51	Ca2+/H+ exchanger 3, standard error	CAX3 std e	±	Bach, Lennart Thomas
52	Na+/H+ exchanger 2	NhaA2		Bach, Lennart Thomas
53	Na+/H+ exchanger 2, standard error	NhaA2 std e	±	Bach, Lennart Thomas
54	Low CO2 induced gene	LCIX		Bach, Lennart Thomas
55	Low CO2 induced gene, standard error	LCIX std e	±	Bach, Lennart Thomas
56	Aquaporin 2	AQP2		Bach, Lennart Thomas
57	Aquaporin 2, standard error	AQP2 std e	±	Bach, Lennart Thomas
58	Glutamic acid, proline, alanine rich protein	GPA		Bach, Lennart Thomas
59	Glutamic acid, proline, alanine rich protein, standard error	GPA std e	±	Bach, Lennart Thomas
60	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
61	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
62	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
63	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
64	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
65	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
66	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
67	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
68	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
69	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1165 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Species	2 Treat	3 Treat	4 E [µmol/m²/s]	5 L:D [hh:hh]	6 Temp [°C]	7 Sal	8 DIC [µmol/kg]	9 Diff (DIC difference from t0 to tfin)	10 pH (free scale)	11 Diff (pH difference from t0 to tfin)	12 [HCO3]- [µmol/kg]	13 Diff (HCO3 difference from t0 to tfin)	14 [CO3]2- [µmol/kg]	15 Diff (CO32 difference from t0 to tfin)	16 CO2 [µmol/kg]	17 Diff (CO2 difference from t0 to tfin)	18 µ [1/day]	19 Diff (growth rate difference from t...)	20 PIC/POC	21 Diff (PIC/POC difference from t0 to...)	22 Chl a [µg/l]	23 Diff (chl a difference from t0 to tfin)	24 POC prod/cell [pg/#/day]	25 Diff (POC proddifference from t0 to...)	26 PIC prod/cell [pg/#/day]	27 Diff (PIC difference from t0 to tfin)	28 C/N	29 Diff (C/N difference from t0 to tfin)	30 AEL1	31 AEL1 std e [±]	32 alpha CA1	33 alpha CA1 std e [±]	34 alpha CA2	35 alpha CA2 std e [±]	36 beta CA	37 beta CA std e [±]	38 gamma CA	39 gamma CA std e [±]	40 delta CA	41 delta CA std e [±]	42 RubisCO	43 RubisCO std e [±]	44 HVCN1	45 HVCN1 std e [±]	46 ATPVc_/c	47 ATPVc_/c std e [±]	48 PATP	49 PATP std e [±]	50 CAX3	51 CAX3 std e [±]	52 NhaA2	53 NhaA2 std e [±]	54 LCIX	55 LCIX std e [±]	56 AQP2	57 AQP2 std e [±]	58 GPA	59 GPA std e [±]	60 CSC flag	61 pH (total scale)	62 CO2 [µmol/kg]	63 pCO2water_SST_wet [µatm]	64 fCO2water_SST_wet [µatm]	65 [HCO3]- [µmol/kg]	66 [CO3]2- [µmol/kg]	67 AT [µmol/kg]	68 Omega Arg	69 Omega Cal
Emiliania huxleyi (phytoplankton)	pH=7.7	1	150	16:08	15	35	199.501	23.048	7.7409	0.007	188.889	21.799	6.960	0.855	3.652	0.394	0.929	0.009	0.034	0.005	0.1380	0.002	5.764	0.034	0.194	0.030	6.7837	0.307	5.540445618	0.430382695				0.507344906	29.438787740	20.045544950	1.381259254	0.186992191	4.212682212	0.099804655	4.032795865	1.045235284	1.545128158	0.424386422	4.435467267	0.480888751	4.399569855	0.899603052	3.575189467	0.444484834	3.047292353	0.222421058	18.743093090	9.661081847	2.216511459	0.734071455	8.544570249	1.231107544	9	7.660	4.000	96	95	189.000	7.000	200.0	0.10	0.15
Emiliania huxleyi (phytoplankton)	pH=7.7	2	150	16:08	15	35	333.803	44.548	7.7473	0.013	315.944	42.002	11.857	1.927	6.001	0.619	1.040	0.007	0.047	0.019	0.1391	0.003	6.608	0.095	0.313	0.122	6.7039	0.231	4.297058879	0.491365806	1.000000000	1.000000000	1.000000000	0.276373082	6.688091973	2.353775311	1.453109439	0.160805627	2.886769125	0.085297674	4.366253599	0.523368459	1.090781275	0.262149044	3.989192742	0.473445342	3.247002896	0.677999336	3.106366848	0.091670718	2.393976060	0.170102956	2.752832642	0.404590609	1.667032868	0.660245239	11.027161630	1.009495743	9	7.670	6.000	158	157	317.000	11.000	334.0	0.17	0.26
Emiliania huxleyi (phytoplankton)	pH=7.7	3	150	16:08	15	35	911.158	13.669	7.7418	0.009	862.675	13.250	31.817	0.306	16.667	0.613	1.098	0.004	0.452	0.008	0.1496	0.009	9.857	0.223	4.459	0.176	7.5064	0.146	1.907792530	0.447788417	1.610528463	0.519348962	1.208562389	0.149160960	20.281728960		1.137387247	0.140305603	1.000000000	0.150330706	2.822523378	0.511029503	1.342817282	0.069328230	1.758520212	0.110744781	1.640533458	0.425786767	1.241316483	0.307825133	1.557380191	0.127074892	5.586024037	4.701834603	2.349664599	0.728410599	7.821283952	1.164720431	9	7.670	16.000	436	435	865.000	30.000	911.0	0.46	0.71
Emiliania huxleyi (phytoplankton)	pH=7.7	4	150	16:08	15	35	2026.128	81.932	7.7359	0.010	1918.748	78.328	69.783	1.164	37.597	2.441	1.063	0.003	0.723	0.041	0.1681	0.004	10.473	0.152	7.573	0.482	7.7965	0.237	1.824450436	0.384248494	2.563844381	1.154904593	1.877389423	0.732221817	42.142630070	31.040436770	1.255291664	0.011871477	1.628541703	0.574777509	2.061275747	0.858244958	1.176093194	0.034970489	2.069653514	0.385478251	2.569122786	0.452099458	1.323960360	0.292361886	1.713329879	0.171113981	34.379701460	26.712388770	2.407757317	1.210806953	5.065218722	0.595086602	9	7.660	37.000	983	980	1924.000	65.000	2026.0	1.00	1.56
Emiliania huxleyi (phytoplankton)	pH=7.7	5	150	16:08	15	35	4059.080	79.726	7.7485	0.016	3842.015	77.939	143.912	2.393	73.152	4.180	1.069	0.006	0.841	0.040	0.1559	0.007	10.208	0.184	8.593	0.555	7.3962	0.231	1.647035652	0.145955737	1.161940249	0.042997942	1.575259570	0.203780645	1.609818157		1.438398687	0.173362538	1.165450767	0.216481270	2.393276412	0.781493427	1.353728900	0.463768074	1.541918573	0.121768576	1.472973660	0.159283342	1.130295097	0.024605201	1.454044152	0.059102641	1.000000000	0.273470442	2.798492966	1.004545812	5.506848952	0.585597016	9	7.670	71.000	1913	1906	3853.000	135.000	4059.0	2.06	3.21
Emiliania huxleyi (phytoplankton)	pH=7.7	6	150	16:08	15	35	7365.156	69.065	7.7412	0.016	6973.245	69.581	256.948	6.750	134.963	6.233	1.017	0.001	0.904	0.037	0.1568	0.023	9.199	0.302	8.318	0.483	7.0881	0.089	1.766822313	0.087684789	1.359129614	0.167236016	1.644657260	0.101183914	3.609163517		1.358012916	0.023334225	1.521884566	0.189010005	1.000000000	0.085812987	1.229496380	0.387818154	1.685770648	0.071806642	1.693654862	0.114172916	1.159239785	0.077449765	1.505015034	0.014385730	1.448811888	0.973064680	1.910073734	0.685646084	4.657437859	0.116794282	9	7.660	132.000	3530	3518	6993.000	240.000	7365.0	3.67	5.72
Emiliania huxleyi (phytoplankton)	pH=8.3	1	150	16:08	15	35	190.238	16.968	8.3283	0.009	165.777	14.349	23.632	2.566	0.829	0.054	0.404	0.002	0.029	0.007	0.1107	0.004	2.776	0.073	0.080	0.018	5.8913	0.171	4.584675918	0.831406103	10.806834980	7.262174370	4.248546329	2.273173623	453.683539000	308.070209500	1.033919749	0.068168561	9.207867019	0.408402748	5.325236423	1.068599967	1.732976725	0.349813861	4.150193981	0.450127405	7.067258033	1.116339887	4.431143302	0.595801733	4.905657600	0.275486819	189.272769500	152.819337300	2.409292593	1.626121512	6.161358892	2.754932532	9	8.250	1.000	22	22	167.000	22.000	190.0	0.34	0.53
Emiliania huxleyi (phytoplankton)	pH=8.3	2	150	16:08	15	35	896.851	29.603	8.3356	0.002	780.067	25.308	112.947	4.189	3.837	0.106	0.948	0.010	0.660	0.011	0.1273	0.008	6.679	0.065	4.410	0.067	6.4372	0.060	2.624917767	0.551637663	2.597469600	0.500891579	1.810499344	0.274177391	17.774783760	3.751163735	1.128455013	0.091709895	2.772472073	0.181027262	2.298179645	0.334620350	1.182840761	0.402918937	2.199838581	0.105934182	2.321630565	0.308643702	1.972649555	0.220223952	1.898835638	0.500352943	16.403350850	3.112160109	1.885403935	0.794928682	8.758886226	1.101835749	9	8.260	4.000	101	101	787.000	106.000	897.0	1.62	2.53
Emiliania huxleyi (phytoplankton)	pH=8.3	3	150	16:08	15	35	2249.720	16.113	8.3364	0.006	1956.294	10.492	283.820	5.711	9.606	0.090	1.078	0.007	0.678	0.043	0.1249	0.017	10.950	0.383	7.416	0.443	6.6255	0.198	1.955432674	0.112225568	1.202027490	0.004776585	1.402533646	0.414870026	5.095925542		1.197350564	0.217348566	1.463456708	0.262425878	2.428355960	0.541524335	1.718202010	0.212845965	1.565164967	0.141632715	1.339930864	0.048746321	1.320579926	0.031788516	1.354397584	0.074397055	2.113969835	0.907403604	1.875077444	0.666984394	7.123613419	0.758392621	9	8.260	9.000	253	252	1973.000	267.000	2250.0	4.08	6.36
Emiliania huxleyi (phytoplankton)	pH=8.3	4	150	16:08	15	35	3927.304	11.426	8.3405	0.001	3411.235	11.050	499.476	0.496	16.593	0.098	1.093	0.011	0.777	0.085	0.1141	0.008	9.587	0.563	7.417	0.367	7.1696	0.435	1.505631707	0.410690668	1.093088561	0.099708597	1.096468916	0.394254053	1.000000000	0.294881198	1.039379424	0.144871053	1.486869873	0.177587438	1.798943159	0.548587273	1.158487034	0.128655642	1.374526614	0.245130877	1.261803620	0.077259867	1.261317045	0.033300642	1.409719765	0.118906576	1.149005621	0.529139169	1.400006082	0.465791729	4.956030138	0.429526001	9	8.260	16.000	437	435	3441.000	470.000	3927.0	7.19	11.19
Emiliania huxleyi (phytoplankton)	pH=8.3	5	150	16:08	15	35	6679.390	34.212	8.3475	0.003	5790.122	34.509	861.552	0.648	27.716	0.351	1.082	0.006	1.024	0.046	0.1223	0.011	8.040	0.417	8.224	0.132	7.3891	0.190	1.567420381	0.171781623	1.261955326	0.080058169	1.270574686	0.149340519	4.974408113		1.290995650	0.096950758	1.842642079	0.232022612	1.626957223	0.856578328	1.047407848	0.183734438	1.434678010	0.035991571	1.609323779	0.185202389	1.674887455	0.192128028	1.325489872	0.017742728	2.779991760	1.168379288	1.323806534	0.527755312	2.975799288	0.660884741	9	8.270	27.000	730	727	5842.000	810.000	6679.0	12.40	19.31
Emiliania huxleyi (phytoplankton)	pH=8.3	6	150	16:08	15	35	9939.234	160.487	8.3274	0.015	8663.685	103.067	1232.106	58.449	43.443	1.029	1.071	0.013	1.044	0.014	0.1240	0.006	8.134	0.459	8.561	0.774	6.9318	0.428	1.759490542	0.124408281	1.027872714	0.030726046	1.381259975	0.478632896	3.737492524	0.369462461	1.099532943	0.077585583	1.577540196	0.190396903	1.578766956	0.631586805	1.488780766	0.578331032	1.272200753	0.190583292	1.546179210	0.405012648	1.428474242	0.128110725	1.314043046	0.165987314	1.981892124	0.148729194	1.477209671	0.631638460	3.305563456	0.323854614	9	8.250	43.000	1144	1140	8739.000	1157.000	9939.0	17.71	27.58
Emiliania huxleyi (phytoplankton)	constant CO2	1	150	16:08	15	35	533.333	15.122	7.5771	0.123	506.081	14.587	12.840	2.976	14.412	3.512	0.897	0.079	0.043	0.025	0.1627	0.012	7.624	0.441	0.336	0.206	6.4499	0.494	2.201577957	0.560004322	2.817076291	0.287682866	1.699527489	0.289068400	30.536824600	11.925429530	1.009696026	0.093340813	3.319950704	0.449883291	1.886128838	0.525965002	1.660817791	0.292745861	2.379067861	0.343423125	1.849114413	0.027097584	2.649092860	0.395061144	1.331449583	0.029263592	18.038827180	5.070634844	1.308296498	0.410816837	3.089777196	0.393939834	9	7.500	14.000	374	372	507.000	12.000	533.0	0.18	0.28
Emiliania huxleyi (phytoplankton)	constant CO2	2	150	16:08	15	35	826.475	12.599	7.6628	0.029	784.196	12.127	24.118	0.345	18.162	0.705	0.964	0.017	0.442	0.015	0.1393	0.014	9.338	0.200	4.131	0.223	6.6178	0.230	1.262222885	0.124959040	1.504237446	0.258238544	1.477829735	0.510476445	15.508294960	6.925067832	1.244850589	0.151318551	1.642968891	0.436680540	1.122662359	0.171465975	1.692448951	0.592733979	1.014578415	0.067974291	1.253360213	0.132885886	1.191144954	0.262689840	1.062445956	0.073391299	10.066647250	4.007191854	1.529149374	0.491460530	2.860155827	1.573649043	9	7.590	18.000	475	474	786.000	23.000	826.0	0.34	0.54
Emiliania huxleyi (phytoplankton)	constant CO2	3	150	16:08	15	35	1174.453	32.073	7.8284	0.034	1107.088	31.355	49.853	0.796	17.512	1.058	1.072	0.011	0.691	0.014	0.1420	0.002	9.361	0.423	6.469	0.224	6.6176	0.098	1.000000000	0.138419835	1.000000000	0.201903187	1.086179467	0.102885490	3.950179568	1.627391437	1.159436258	0.180679465	1.149538738	0.220932781	2.242951733	0.384719368	1.000000000	0.101638177	1.140842775	0.268396173	1.165753063	0.221732283	1.000000000	0.165260174	1.000000000	0.203226405	3.242350126	0.930404027	1.226211173	0.201243777	4.507291241	0.749627443	9	7.750	17.000	459	457	1111.000	47.000	1174.0	0.71	1.11
Emiliania huxleyi (phytoplankton)	constant CO2	4	150	16:08	15	35	1911.562	17.430	8.0893	0.011	1752.474	13.870	143.886	3.688	15.202	0.127	1.117	0.023	0.924	0.074	0.1208	0.004	8.578	0.525	7.906	0.247	6.6644	0.241	1.505310771	0.191963663	1.025503974	0.053937127	1.000000000	0.195095748	4.664009582	1.810930772	1.000000000	0.091568869	1.915271507	0.150140632	1.381299178	0.296653357	1.033982549	0.212437181	1.000000000	0.028651958	1.724089386	0.536725844	1.329749005	0.080385207	1.072244364	0.028371445	4.838595013	1.494156012	1.000000000	0.395249656	1.283223069	0.265677359	9	8.010	15.000	399	398	1762.000	135.000	1912.0	2.06	3.21
Emiliania huxleyi (phytoplankton)	constant CO2	5	150	16:08	15	35	4112.138	22.047	8.3878	0.009	3521.816	8.938	574.957	14.462	15.365	0.291	1.136	0.069	1.113	0.039	0.1262	0.009	8.618	0.287	9.595	0.550	6.9744	0.230	3.240287291	0.789683420	2.095791331	0.342557854	2.032900688	0.213998116	6.924993719	4.231460396	1.782706194	0.253619869	3.503071804	0.207147476	1.520684295	0.729979490	1.753847790	0.316552247	2.192679309	0.298902805	2.648060887	0.307091801	2.328949749	0.440345295	2.199049538	0.410531857	5.073803593	1.484966838	2.617153570	0.649155337	1.057991001	0.563310387	9	8.310	15.000	405	404	3556.000	541.000	4112.0	8.28	12.89