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Borchard, Corinna; Engel, Anja (2012): Experiment: Organic matter exudation by Emiliania huxleyi under simulated future ocean conditions. PANGAEA,, Supplement to: Borchard, C; Engel, A (2012): Organic matter exudation by Emiliania huxleyi under simulated future ocean conditions. Biogeosciences, 9(8), 3405-3423,

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Emiliania huxleyi (strain B 92/11) was exposed to different nutrient supply, CO2 and temperature conditions in phosphorus controlled chemostats to investigate effects on organic carbon exudation and partitioning between the pools of particulate organic carbon (POC) and dissolved organic carbon (DOC). 14C incubation measurements for primary production (PP) and extracellular release (ER) were performed. Chemical analysis included the amount and composition of high molecular weight (>1 kDa) dissolved combined carbohydrates (HMW-dCCHO), particulate combined carbohydrates (pCCHO) and the carbon content of transparent exopolymer particles (TEP-C). Applied CO2 and temperature conditions were 300, 550 and 900 µatm pCO2 at 14 °C, and additionally 900 µatm pCO2 at 18 °C simulating a greenhouse ocean scenario.
Enhanced nutrient stress by reducing the dilution rate (D) from D = 0.3 /d to D = 0.1 /d (D = µ) induced the strongest response in E. huxleyi. At µ = 0.3 /d, PP was significantly higher at elevated CO2 and temperature and DO14C production correlated to PO14C production in all treatments, resulting in similar percentages of extracellular release (PER; (DO14C production/PP) × 100) averaging 3.74 ± 0.94%. At µ = 0.1 /d, PO14C production decreased significantly, while exudation of DO14C increased. Thus, indicating a stronger partitioning from the particulate to the dissolved pool. Maximum PER of 16.3 ± 2.3% were observed at µ = 0.1 /d at elevated CO2 and temperature.
While cell densities remained constant within each treatment and throughout the experiment, concentrations of HMW-dCCHO, pCCHO and TEP were generally higher under enhanced nutrient stress. At µ= 0.3 /d, pCCHO concentration increased significantly with elevated CO2 and temperature. At µ = 0.1 /d, the contribution (mol % C) of HMW-dCCHO to DOC was lower at elevated CO2 and temperature while pCCHO and TEP concentrations were higher. This was most pronounced under greenhouse conditions. Our findings suggest a stronger transformation of primary produced DOC into POC by coagulation of exudates under nutrient limitation. Our results further imply that elevated CO2 and temperature will increase exudation by E. huxleyi and may affect organic carbon partitioning in the ocean due to an enhanced transfer of HMW-dCCHO to TEP by aggregation processes.
Bottles or small containers/Aquaria (<20 L); Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Other metabolic rates; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Temperature
Further details:
Lavigne, Héloise; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4.
Seventh Framework Programme (FP7), grant/award no. 211384: European Project on Ocean Acidification
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-02-11.
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1SpeciesSpeciesBorchard, Corinna
2ReplicateReplicateBorchard, Corinna
3TreatmentTreatBorchard, Corinna
4Growth rateµ1/dayBorchard, Corinna
5Exudation as determined by 14C DOC productionExud 14C DOCµmol/l/dayBorchard, Corinna
6Primary production of POC as determined by 14C POC productionPP 14C POCµmol/l/dayBorchard, Corinna
7Primary production of carbon per dayPP Cµmol/l/dayBorchard, Corinna
8Extracellular releaseExtrac rel%Borchard, Corinna
9Carbohydrates, dissolved hydrolyzableDHCHOµmol/lBorchard, Corinna
10Carbohydrates, total combinedTCCHOµmol/lBorchard, Corinna
11Carbohydrates, particulate hydrolyzablePCCHOµmol/lBorchard, Corinna
12FucoseFucµmol/lBorchard, Corinnadissolved
13RhamnoseRhaµmol/lBorchard, Corinnadissolved
14Arabinose/GalactosamineAra/GalNµmol/lBorchard, Corinnadissolved
15GlucosamineGlcNµmol/lBorchard, Corinnadissolved
16GalactoseGalµmol/lBorchard, Corinnadissolved
17GlucoseGlcµmol/lBorchard, Corinnadissolved
18Mannose/XyloseMan/Xylµmol/lBorchard, Corinnadissolved
19Galacturonic acidGalAµmol/lBorchard, Corinnadissolved
20Glucuronic acidGlcAµmol/lBorchard, Corinnadissolved
21FucoseFucµmol/lBorchard, Corinnaparticulate
22RhamnoseRhaµmol/lBorchard, Corinnaparticulate
23Arabinose/GalactosamineAra/GalNµmol/lBorchard, Corinnaparticulate
24GlucosamineGlcNµmol/lBorchard, Corinnaparticulate
25GalactoseGalµmol/lBorchard, Corinnaparticulate
26GlucoseGlcµmol/lBorchard, Corinnaparticulate
27Mannose/XyloseMan/Xylµmol/lBorchard, Corinnaparticulate
28Galacturonic acidGalAµmol/lBorchard, Corinnaparticulate
29Glucuronic acidGlcAµmol/lBorchard, Corinnaparticulate
30Transparent exopolymer particlesTEPµmol/lBorchard, Corinna
31Carbon, organic, dissolvedDOCµmol/lBorchard, Corinna
32Transparent exopolymer particles/particulate organic carbon ratioTEP/POCBorchard, Corinna
33SalinitySalBorchard, Corinna
34Temperature, waterTemp°CBorchard, Corinna
35Alkalinity, totalATµmol/kgBorchard, Corinna
36pHpHBorchard, CorinnaNBS scale
37Carbon, inorganic, dissolvedDICµmol/kgBorchard, Corinna
38Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmBorchard, Corinna
39Carbon dioxideCO2µmol/kgBorchard, Corinna
40Bicarbonate ion[HCO3]-µmol/kgBorchard, Corinna
41Carbonate ion[CO3]2-µmol/kgBorchard, Corinna
42Calcite saturation stateOmega CalBorchard, Corinna
43Revelle factorRBorchard, Corinna
44Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
45pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
46Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
47Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
48Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
49Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
50Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
51Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
52Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
53Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
1269 data points

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