Citation:

bg-9-3405-2012

Name: Experiment: Organic matter exudation by Emiliania huxleyi under simulated future ocean conditions
Published: 2012
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Bottles or small containers/Aquaria (<20 L); Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Other metabolic rates; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Temperature

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Abstract:

Emiliania huxleyi (strain B 92/11) was exposed to different nutrient supply, CO2 and temperature conditions in phosphorus controlled chemostats to investigate effects on organic carbon exudation and partitioning between the pools of particulate organic carbon (POC) and dissolved organic carbon (DOC). 14C incubation measurements for primary production (PP) and extracellular release (ER) were performed. Chemical analysis included the amount and composition of high molecular weight (>1 kDa) dissolved combined carbohydrates (HMW-dCCHO), particulate combined carbohydrates (pCCHO) and the carbon content of transparent exopolymer particles (TEP-C). Applied CO2 and temperature conditions were 300, 550 and 900 µatm pCO2 at 14 °C, and additionally 900 µatm pCO2 at 18 °C simulating a greenhouse ocean scenario.

Enhanced nutrient stress by reducing the dilution rate (D) from D = 0.3 /d to D = 0.1 /d (D = µ) induced the strongest response in E. huxleyi. At µ = 0.3 /d, PP was significantly higher at elevated CO2 and temperature and DO14C production correlated to PO14C production in all treatments, resulting in similar percentages of extracellular release (PER; (DO14C production/PP) × 100) averaging 3.74 ± 0.94%. At µ = 0.1 /d, PO14C production decreased significantly, while exudation of DO14C increased. Thus, indicating a stronger partitioning from the particulate to the dissolved pool. Maximum PER of 16.3 ± 2.3% were observed at µ = 0.1 /d at elevated CO2 and temperature.

While cell densities remained constant within each treatment and throughout the experiment, concentrations of HMW-dCCHO, pCCHO and TEP were generally higher under enhanced nutrient stress. At µ= 0.3 /d, pCCHO concentration increased significantly with elevated CO2 and temperature. At µ = 0.1 /d, the contribution (mol % C) of HMW-dCCHO to DOC was lower at elevated CO2 and temperature while pCCHO and TEP concentrations were higher. This was most pronounced under greenhouse conditions. Our findings suggest a stronger transformation of primary produced DOC into POC by coagulation of exudates under nutrient limitation. Our results further imply that elevated CO2 and temperature will increase exudation by E. huxleyi and may affect organic carbon partitioning in the ocean due to an enhanced transfer of HMW-dCCHO to TEP by aggregation processes.

Keyword(s):

Bottles or small containers/Aquaria (<20 L); Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Other metabolic rates; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Temperature

Further details:

Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb

Project(s):

Biological Impacts of Ocean Acidification (BIOACID)

European Project on Ocean Acidification (EPOCA)

Ocean Acidification International Coordination Centre (OA-ICC)

Funding:

Seventh Framework Programme (FP7), grant/award no. 211384 : European Project on Ocean Acidification

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-02-11.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Borchard, Corinna
2	Replicate	Repl		Borchard, Corinna
3	Treatment	Treat		Borchard, Corinna
4	Growth rate	µ	1/day	Borchard, Corinna
5	Exudation as determined by 14C DOC production	Exud 14C DOC	µmol/l/day	Borchard, Corinna
6	Primary production of POC as determined by 14C POC production	PP 14C POC	µmol/l/day	Borchard, Corinna
7	Primary production of carbon per day	PP C	µmol/l/day	Borchard, Corinna
8	Extracellular release	Extrac rel	%	Borchard, Corinna
9	Carbohydrates, dissolved hydrolyzable	DHCHO	µmol/l	Borchard, Corinna
10	Carbohydrates, total combined	TCCHO	µmol/l	Borchard, Corinna
11	Carbohydrates, particulate hydrolyzable	PCCHO	µmol/l	Borchard, Corinna
12	Fucose	Fuc	µmol/l	Borchard, Corinna		dissolved
13	Rhamnose	Rha	µmol/l	Borchard, Corinna		dissolved
14	Arabinose/Galactosamine	Ara/GalN	µmol/l	Borchard, Corinna		dissolved
15	Glucosamine	GlcN	µmol/l	Borchard, Corinna		dissolved
16	Galactose	Gal	µmol/l	Borchard, Corinna		dissolved
17	Glucose	Glc	µmol/l	Borchard, Corinna		dissolved
18	Mannose/Xylose	Man/Xyl	µmol/l	Borchard, Corinna		dissolved
19	Galacturonic acid	GalA	µmol/l	Borchard, Corinna		dissolved
20	Glucuronic acid	GlcA	µmol/l	Borchard, Corinna		dissolved
21	Fucose	Fuc	µmol/l	Borchard, Corinna		particulate
22	Rhamnose	Rha	µmol/l	Borchard, Corinna		particulate
23	Arabinose/Galactosamine	Ara/GalN	µmol/l	Borchard, Corinna		particulate
24	Glucosamine	GlcN	µmol/l	Borchard, Corinna		particulate
25	Galactose	Gal	µmol/l	Borchard, Corinna		particulate
26	Glucose	Glc	µmol/l	Borchard, Corinna		particulate
27	Mannose/Xylose	Man/Xyl	µmol/l	Borchard, Corinna		particulate
28	Galacturonic acid	GalA	µmol/l	Borchard, Corinna		particulate
29	Glucuronic acid	GlcA	µmol/l	Borchard, Corinna		particulate
30	Transparent exopolymer particles	TEP	µmol/l	Borchard, Corinna
31	Carbon, organic, dissolved	DOC	µmol/l	Borchard, Corinna
32	Transparent exopolymer particles/Carbon, organic, particulate ratio	TEP/POC		Borchard, Corinna
33	Salinity	Sal		Borchard, Corinna
34	Temperature, water	Temp	°C	Borchard, Corinna
35	Alkalinity, total	AT	µmol/kg	Borchard, Corinna
36	pH	pH		Borchard, Corinna		NBS scale
37	Carbon, inorganic, dissolved	DIC	µmol/kg	Borchard, Corinna
38	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Borchard, Corinna
39	Carbon dioxide	CO2	µmol/kg	Borchard, Corinna
40	Bicarbonate ion	[HCO3]-	µmol/kg	Borchard, Corinna
41	Carbonate ion	[CO3]2-	µmol/kg	Borchard, Corinna
42	Calcite saturation state	Omega Cal		Borchard, Corinna
43	Revelle factor	R		Borchard, Corinna
44	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
46	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
50	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
51	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
52	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
53	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1269 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Species	2 Repl	3 Treat	4 µ [1/day]	5 Exud 14C DOC [µmol/l/day]	6 PP 14C POC [µmol/l/day]	7 PP C [µmol/l/day]	8 Extrac rel [%]	9 DHCHO [µmol/l]	10 TCCHO [µmol/l]	11 PCCHO [µmol/l]	12 Fuc [µmol/l] (dissolved)	13 Rha [µmol/l] (dissolved)	14 Ara/GalN [µmol/l] (dissolved)	15 GlcN [µmol/l] (dissolved)	16 Gal [µmol/l] (dissolved)	17 Glc [µmol/l] (dissolved)	18 Man/Xyl [µmol/l] (dissolved)	19 GalA [µmol/l] (dissolved)	20 GlcA [µmol/l] (dissolved)	21 Fuc [µmol/l] (particulate)	22 Rha [µmol/l] (particulate)	23 Ara/GalN [µmol/l] (particulate)	24 GlcN [µmol/l] (particulate)	25 Gal [µmol/l] (particulate)	26 Glc [µmol/l] (particulate)	27 Man/Xyl [µmol/l] (particulate)	28 GalA [µmol/l] (particulate)	29 GlcA [µmol/l] (particulate)	30 TEP [µmol/l]	31 DOC [µmol/l]	32 TEP/POC	33 Sal	34 Temp [°C]	35 AT [µmol/kg]	36 pH (NBS scale)	37 DIC [µmol/kg]	38 pCO2water_SST_wet [µatm]	39 CO2 [µmol/kg]	40 [HCO3]- [µmol/kg]	41 [CO3]2- [µmol/kg]	42 Omega Cal	43 R	44 CSC flag	45 pH (total scale)	46 CO2 [µmol/kg]	47 pCO2water_SST_wet [µatm]	48 fCO2water_SST_wet [µatm]	49 [HCO3]- [µmol/kg]	50 [CO3]2- [µmol/kg]	51 DIC [µmol/kg]	52 Omega Arg	53 Omega Cal
Emiliania huxleyi (phytoplankton)	1	300-14	0.3	5.36	135.89	141.25	3.79	9.90	37.05	27.15	0.27	0.60	1.52	0.19	1.16	2.37	1.89	0.71	1.20	0.17	1.61	0.66	0.12	2.53	15.94	1.23	3.46	1.43	10.50	17.60	2.35	31.5	14	1805.192308	8.326	1574.9326600	205.4492647	8.062927029	1415.8658040	151.00392920	3.678861913	9.721401358	26	8.210	8.000000000	205.0000000	204	1416.0000000	151.00000000	1575.0000000	2.336759030	3.672981779
Emiliania huxleyi (phytoplankton)	2	300-14	0.3	6.58	159.64	166.22	3.96	7.36	44.66	37.30	0.19	0.14	1.55	0.10	0.60	2.25	1.24	0.46	0.84	0.26	0.65	0.83	0.06	1.42	30.03	0.08	2.34	1.62	5.16	21.62	0.98	31.5	14	1459.113712	8.211	1299.9211570	225.8756635	8.864568073	1193.4676660	97.58892344	2.377528687	10.343351130	26	8.090	9.000000000	226.0000000	225	1194.0000000	97.00000000	1300.0000000	1.509710898	2.373004896
Emiliania huxleyi (phytoplankton)	3	300-14	0.3	7.95	147.48	155.43	5.12	7.73	40.51	32.78	0.19	0.15	1.67	0.11	0.71	1.58	1.67	0.49	1.16	0.38	0.95	0.82	0.05	1.73	25.17	0.47	2.31	0.89	18.88	26.13	3.90	31.5	14	1420.116279	8.149	1283.3140330	259.3067808	10.176583760	1188.7945730	84.34287634	2.054819348	11.021889550	26	8.030	10.000000000	259.0000000	258	1189.0000000	84.00000000	1284.0000000	1.304702941	2.050767781
Emiliania huxleyi (phytoplankton)	4	300-14	0.1	7.38	76.08	83.46	8.84	25.86	93.39	67.53	0.80	1.74	4.92	0.30	2.24	4.19	6.17	4.48	1.03	0.58	3.31	1.54	0.24	4.04	48.35	5.60	2.91	0.97	47.33	75.45	6.09	31.5	14	1053.837209	7.961	980.6071286	311.5748441	12.227861880	925.8069629	42.57230383	1.037175840	12.290258460	26	7.840	12.000000000	311.0000000	310	926.0000000	42.00000000	981.0000000	0.658203983	1.034583030
Emiliania huxleyi (phytoplankton)	5	300-14	0.1	13.28	77.53	90.81	14.62	27.18	91.29	64.17	0.79	1.84	5.39	0.28	2.41	4.88	5.94	3.02	2.63	0.48	2.43	1.37	0.26	3.08	46.08	5.98	4.49		70.45	135.70	8.89	31.5	14	1020.348837	8.015	936.7214863	261.2853873	10.254234860	880.5437101	45.92354129	1.118820999	11.383343270	26	7.900	10.000000000	261.0000000	260	881.0000000	46.00000000	937.0000000	0.710085814	1.116132311
Emiliania huxleyi (phytoplankton)	6	300-14	0.1	7.59	137.09	144.68	5.25	24.46	102.07	77.61	0.78	1.83	5.54	0.30	2.31	3.35	5.72	2.71	1.91	0.75	3.24	1.77	0.21	4.78	51.42	8.95	6.27	0.20	68.83	77.59	4.91	31.5	14	1019.690722	8.029	917.5791934	247.9421329	9.730574252	861.5219559	46.32666327	1.128642135	11.001156320	26	7.910	10.000000000	252.0000000	251	876.0000000	47.00000000	933.0000000	0.728525480	1.145116283
Emiliania huxleyi (phytoplankton)	1	550-14	0.3	6.56	174.43	180.99	3.63	6.84	35.80	28.96	0.18	0.14	0.82	0.09	0.17	3.37	0.58	0.14	1.36	0.02	0.50	0.33	0.04	1.02	24.82	0.65	1.32	0.26	9.68	21.56	1.69	31.5	14	1815.394737	8.040	1694.7430410	445.7315447	17.492887720	1589.5275690	87.72258344	2.137158104	13.544260100	26	7.920	17.000000000	445.0000000	443	1590.0000000	88.00000000	1695.0000000	1.356936104	2.132869295
Emiliania huxleyi (phytoplankton)	2	550-14	0.3	4.82	170.61	175.43	2.75	9.54	49.95	40.42	0.23	0.22	1.52	0.11	0.63	2.45	2.15	0.63	1.61	0.34	0.42	1.07	0.21	1.80	32.40	0.14	3.08	0.95	16.31	15.85	2.91	31.5	14	1423.885135	7.885	1357.6774160	515.5446347	20.232726440	1287.6718110	49.77287868	1.212601213	14.787738910	26	7.770	20.000000000	515.0000000	513	1288.0000000	50.00000000	1358.0000000	0.769646274	1.209751072
Emiliania huxleyi (phytoplankton)	3	550-14	0.3	8.27	204.75	213.02	3.88	10.42	48.32	37.90	0.21	0.14	1.77	0.05	0.90	2.97	2.33	0.61	1.44	0.44	0.84	1.00	0.16	1.92	28.34	0.70	3.35	1.14	21.95	27.19	3.26	31.5	14	1445.625000	7.882	1379.7074820	528.0600697	20.723898990	1308.7838740	50.19970858	1.222999937	14.903001330	26	7.760	21.000000000	527.0000000	526	1309.0000000	50.00000000	1380.0000000	0.776262180	1.220150134
Emiliania huxleyi (phytoplankton)	4	550-14	0.1	6.86	62.31	69.17	9.92	21.90	85.35	63.44	0.75	1.05	4.44	0.35	2.40	4.77	5.57	1.39	1.18	0.43	3.59	0.90	0.16	2.98	43.35	4.61	6.06	1.37	42.48	70.13	6.67	31.5	14	1045.419463	7.736	1013.6627530	545.4109637	21.404840790	965.7917038	26.46620859	0.644788035	15.046542460	26	7.620	21.000000000	545.0000000	543	966.0000000	26.00000000	1014.0000000	0.409070470	0.642988158
Emiliania huxleyi (phytoplankton)	5	550-14	0.1	8.35	111.44	119.78	6.97	22.21	94.69	72.47	0.61	0.75	3.83	0.20	2.32	8.11	4.09	1.12	1.21	0.64	4.17	2.03	0.30	3.15	48.19	7.04	5.85	1.10	55.75	85.34	4.89	31.5	14	1095.100671	7.760	1058.9558010	539.1094764	21.157536750	1008.5966580	29.20160607	0.711429675	15.044174670	26	7.640	21.000000000	539.0000000	537	1009.0000000	29.00000000	1059.0000000	0.451368285	0.709473019
Emiliania huxleyi (phytoplankton)	6	550-14	0.1	9.63	111.67	121.30	7.94	21.58	83.32	61.74	0.81	0.90	4.55	0.32	2.25	6.33	4.15	1.40	0.88	0.42	3.34	1.57	0.12	2.47	42.02	6.37	4.93	0.51	70.71	98.46	6.19	31.5	14	1145.948276	7.765	1099.2791300	552.8313279	21.696055530	1046.9021670	30.68090779	0.747469445	15.070597600	26	7.650	22.000000000	557.0000000	555	1056.0000000	31.00000000	1109.0000000	0.478234316	0.751701783
Emiliania huxleyi (phytoplankton)	1	900-14	0.3	7.82	204.27	212.10	3.69	8.37	39.48	31.13	0.17	0.25	1.24	0.11	0.38	3.73	1.41	0.27	0.82	0.13	0.44		-0.02	0.91	27.30	0.06	1.62	0.68	8.12	25.71	1.21	31.5	14	1873.303887	7.847	1808.2120320	751.6989613	29.500684180	1717.9502160	60.76113148	1.480304609	16.491343660	26	7.730	29.000000000	751.0000000	748	1718.0000000	61.00000000	1808.0000000	0.939612172	1.476908121
Emiliania huxleyi (phytoplankton)	2	900-14	0.3	6.16	176.40	182.56	3.37	10.83	68.81	57.98	0.26	0.38	2.37	0.11	0.65	4.07	1.24	0.82	0.93	0.35	1.84	0.91	0.07	1.60	49.14	1.27	2.24	0.57	12.37	20.23	2.13	31.5	14	1522.154605	7.715	1492.5029190	842.2263983	33.053464570	1422.2796890	37.16976543	0.905555472	16.925748850	26	7.600	33.000000000	841.0000000	838	1422.0000000	37.00000000	1493.0000000	0.574631860	0.903222079
Emiliania huxleyi (phytoplankton)	3	900-14	0.3	9.30	186.68	195.98	4.74	12.22	71.15	58.92	0.24	0.48	2.42	0.12	0.78	5.48	1.49	0.54	0.68	0.56	2.10	0.90	0.07	2.11	48.30	1.42	2.86	0.61	8.80	30.54	1.43	31.5	14	1512.431507	7.640	1498.7807170	1005.1947720	39.449214430	1427.9395680	31.39193515	0.764791984	17.216058230	26	7.520	39.000000000	1004.0000000	1001	1428.0000000	31.00000000	1499.0000000	0.485235763	0.762706849
Emiliania huxleyi (phytoplankton)	4	900-14	0.1	10.51	81.31	91.82	11.44	19.51	109.06	89.54	0.63	1.57	4.51	0.28	2.25	2.98	4.37	1.66	1.27	0.92	4.57	1.95	0.10	4.97	61.66	7.22	6.57	1.59	69.77	107.40	8.39	31.5	14	1236.838235	7.536	1240.6131070	1055.0890210	41.407331370	1178.8232980	20.38247748	0.496571980	16.356558610	26	7.420	41.000000000	1054.0000000	1051	1179.0000000	20.00000000	1241.0000000	0.314982590	0.495098253
Emiliania huxleyi (phytoplankton)	5	900-14	0.1	5.57	91.64	97.21	5.73	18.63	101.92	83.29	0.59	1.19	4.07	0.17	1.62	3.60	4.45	1.71	1.22	0.91	4.57	2.60	0.26	5.42	53.59	7.94	6.64	1.38	57.32	56.92	6.12	31.5	14	1269.174757	7.544	1272.0035970	1062.1912750	41.686061780	1209.0207790	21.29675595	0.518846263	16.476299870	26	7.430	42.000000000	1061.0000000	1058	1209.0000000	21.00000000	1272.0000000	0.329124144	0.517326335
Emiliania huxleyi (phytoplankton)	6	900-14	0.1	14.93	86.64	101.56	14.70	21.61	103.51	81.89	0.64	1.48	4.54	0.21	2.21	5.66	4.49	1.47	0.91	0.81	3.68	2.45	0.29	4.04	54.97	7.74	6.33	1.60	109.17	152.57	12.33	31.5	14	1274.700000	7.540	1247.0255770	1050.7982050	41.238936840	1185.1023300	20.68430990	0.503925429	16.006176100	26	7.420	42.000000000	1076.0000000	1072	1215.0000000	21.00000000	1278.0000000	0.327694284	0.515078841
Emiliania huxleyi (phytoplankton)	1	900-18	0.3	7.89	263.61	271.51	2.91	9.97	63.95	55.03	0.25	0.32	1.71	0.09	0.49	3.30	1.97	0.55	1.28	0.34	1.29	0.60	0.03	1.18	47.07		3.49	1.03	26.11	25.94	3.01	31.5	18	1384.534884	7.699	1349.0948080	825.6082886	28.756570800	1283.3559510	36.98228552	0.904627352	15.974199040	26	7.570	29.000000000	825.0000000	822	1284.0000000	37.00000000	1349.0000000	0.579167043	0.902274636
Emiliania huxleyi (phytoplankton)	2	900-18	0.3	6.15	204.61	210.76	2.92	11.76	80.31	68.54	0.24	0.55	2.62	0.11	0.86	3.67	1.73	0.62	1.36	0.54	2.82	1.25	0.05	2.94	52.36	3.96	4.30	0.32	34.39	20.20	5.12	31.5	18	1292.768456	7.676	1262.4840140	815.2484511	28.395729710	1201.2736580	32.81462602	0.802681820	15.861245420	26	7.550	28.000000000	814.0000000	811	1201.0000000	33.00000000	1263.0000000	0.513872547	0.800553434
Emiliania huxleyi (phytoplankton)	3	900-18	0.3	11.73	222.79	234.52	5.00	12.69	74.60	61.90	0.34	0.34	2.31	0.19	0.91	4.62	2.45	0.72	0.82	0.46	2.52	1.22	0.05	1.44	50.15	1.70	3.55	0.82	34.79	38.54	4.75	31.5	18	1325.317726	7.608	1308.3451610	988.3134354	34.423716030	1244.8534360	29.06800856	0.711035439	16.322923870	26	7.480	34.000000000	987.0000000	984	1245.0000000	29.00000000	1308.0000000	0.455128904	0.709037696
Emiliania huxleyi (phytoplankton)	4	900-18	0.1	15.41	73.88	89.28	17.25	19.41	89.05	69.64	0.71	0.74	4.60	0.15	1.86	3.55	5.12	1.63	1.05	0.75	2.02	1.64	0.10	2.96	51.20	5.17	4.66	1.13	99.10	157.48	13.12	31.5	18	1133.154362	7.539	1127.4444060	998.1297482	34.765625740	1071.3602340	21.31854593	0.521475065	15.810840230	26	7.410	35.000000000	997.0000000	994	1072.0000000	21.00000000	1128.0000000	0.333752605	0.519947593
Emiliania huxleyi (phytoplankton)	5	900-18	0.1	18.82	85.80	104.62	17.99	23.09	101.95	78.86	0.77	0.89	5.04	0.16	2.12	3.47	6.32	2.77	1.56	0.82	1.60	1.61	0.11	2.54	59.06	4.89	8.05	0.18	84.30	192.40	9.61	31.5	18	1173.322148	7.553	1165.5934330	999.3106372	34.806757020	1108.0115090	22.77516682	0.557105613	15.944020900	26	7.430	35.000000000	998.0000000	995	1108.0000000	23.00000000	1166.0000000	0.356575290	0.555502671
Emiliania huxleyi (phytoplankton)	6	900-18	0.1	16.94	107.37	124.31	13.63	19.60	119.57	99.97	1.34	0.47	4.69	0.13	2.10	3.75	4.87	1.66	0.60	0.77	1.25	2.62	0.25	2.28	70.24	11.09	10.37	1.10	106.77	173.21	9.73	31.5	18	1152.516892	7.549	1139.8950420	985.4026478	34.322331060	1083.4872040	22.08550743	0.540235786	15.789898610	26	7.420	34.000000000	989.0000000	986	1089.0000000	22.00000000	1145.0000000	0.347358076	0.541143328