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Müller, Marius N (2014): Experiment: Influence of CO2 and nitrogen limitation on the coccolith volume of Emiliania huxleyi (Haptophyta). PANGAEA, https://doi.org/10.1594/PANGAEA.829376, Supplement to: Müller, Marius N; Beaufort, Luc; Bernard, O; Pedrotti, Maria-Luiza; Talec, A; Sciandra, Antoine (2012): Influence of CO2 and nitrogen limitation on the coccolith volume of Emiliania huxleyi (Haptophyta). Biogeosciences, 9(10), 4155-4167, https://doi.org/10.5194/bg-9-4155-2012

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Abstract:
Coccolithophores, a key phytoplankton group, are one of the most studied organisms regarding their physiological response to ocean acidification/carbonation. The biogenic production of calcareous coccoliths has made coccolithophores a promising group for paleoceanographic research aiming to reconstruct past environmental conditions. Recently, geochemical and morphological analyses of fossil coccoliths have gained increased interest in regard to changes in seawater carbonate chemistry. The cosmopolitan coccolithophore Emiliania huxleyi (Lohm.) Hay and Mohler was cultured over a range of pCO2 levels in controlled laboratory experiments under nutrient replete and nitrogen limited conditions. Measurements of photosynthesis and calcification revealed, as previously published, an increase in particulate organic carbon production and a moderate decrease in calcification from ambient to elevated pCO2. The enhancement in particulate organic carbon production was accompanied by an increase in cell diameter. Changes in coccolith volume were best correlated with the coccosphere/cell diameter and no significant correlation was found between the coccolith volume and the particulate inorganic carbon production. The conducted experiments revealed that the coccolith volume of E. huxleyi is variable with aquatic CO2 concentration but its sensitivity is rather small in comparison with its sensitivity to nitrogen limitation. Comparing coccolith morphological and geometrical parameters like volume, mass and size to physiological parameters under controlled laboratory conditions is an important step to understand variations in fossil coccolith geometry.
Keyword(s):
Bottles or small containers/Aquaria ( 20 L); Calcification/Dissolution; Chromista; Emiliania huxleyi; Gene expression (incl. proteomics); Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Macro-nutrients; North Atlantic; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species
Further details:
Lavigne, Héloise; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-02-11.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1SpeciesSpeciesMüller, Marius N
2Experimental treatmentExp treatMüller, Marius N
3ExperimentExpMüller, Marius N
4DescriptionDescriptionMüller, Marius N
5CodeCodeMüller, Marius N
6Carbon, inorganic, dissolvedDICµmol/kgMüller, Marius N
7Standard deviationStd dev±Müller, Marius N
8Alkalinity, totalATµmol/kgMüller, Marius N
9Alkalinity, total, standard deviationAT std dev±Müller, Marius N
10Carbon dioxide, partial pressurepCO2µatmMüller, Marius N
11Carbon dioxide, partial pressure, standard deviationpCO2 std dev±Müller, Marius N
12pHpHMüller, Marius Ntotal scale
13pH, standard deviationpH std dev±Müller, Marius Ntotal scale
14Calcite saturation stateOmega CalMüller, Marius N
15Calcite saturation state, standard deviationOmega Cal std dev±Müller, Marius N
16Carbon dioxideCO2µmol/kgMüller, Marius N
17Carbon dioxide, standard deviationCO2 std dev±Müller, Marius N
18Bicarbonate ion[HCO3]-µmol/kgMüller, Marius N
19Bicarbonate ion, standard deviation[HCO3]- std dev±Müller, Marius N
20Carbonate ion[CO3]2-µmol/kgMüller, Marius N
21Carbonate ion, standard deviation[CO3]2- std dev±Müller, Marius N
22Cell densityCells#/mlMüller, Marius Ncells/ml x 10**4
23Cell density, standard deviationCells std dev±Müller, Marius Ncells/ml x 10**4
24Growth rateµ1/dayMüller, Marius N
25Growth rate, standard deviationµ std dev±Müller, Marius N
26Particulate inorganic carbon production per cellPIC prodpg/#/dayMüller, Marius N
27Particulate inorganic carbon, production, standard deviationPIC prod std dev±Müller, Marius N
28Production of particulate organic carbon per cellPOC prodpg/#/dayMüller, Marius N
29Particulate organic carbon, production, standard deviationPOC prod std dev±Müller, Marius N
30Nitrogen, total particulate production per cellTPN prodpg/#/dayMüller, Marius N
31Nitrogen, total particulate production standard deviationTPN prod std dev±Müller, Marius N
32Production of particulate organic phosphorus, per cellPOP prodpmol/#/dayMüller, Marius N
33Standard deviationStd dev±Müller, Marius N
34Particulate inorganic carbon/particulate organic carbon ratioPIC/POCMüller, Marius N
35Particulate inorganic carbon/particulate organic carbon ratio, standard deviationPIC/POC ratio std dev±Müller, Marius N
36Carbon, organic, particulate/Nitrogen, particulate ratioPOC/PNMüller, Marius N
37Standard deviationStd dev±Müller, Marius N
38Particulate organic carbon/particulate organic phosphorus ratioPOC/POPMüller, Marius N
39Standard deviationStd dev±Müller, Marius N
40Nitrogen, total particulate/Phosphorus, organic particulate ratioTPN/POPMüller, Marius N
41Standard deviationStd dev±Müller, Marius N
42Coccoliths, other, diameterCocco diamµmMüller, Marius N
43Standard deviationStd dev±Müller, Marius N
44Cell sizeCell sizeµmMüller, Marius N
45Cell size, standard deviationCell size std dev±Müller, Marius N
46Cell biovolumeCell biovolµm3Müller, Marius N
47Standard deviationStd dev±Müller, Marius N
48SalinitySalMüller, Marius N
49Temperature, waterTemp°CMüller, Marius N
50Temperature, standard deviationT std dev±Müller, Marius N
51Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
52pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
53Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
54Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
55Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
56Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
57Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
58Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
59Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Size:
397 data points

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