Citation:

bg-9-4155-2012

Name: Experiment: Influence of CO2 and nitrogen limitation on the coccolith volume of Emiliania huxleyi (Haptophyta)
Creator: Marius N Müller
Published: 2012
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Emiliania huxleyi; Gene expression (incl. proteomics); Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Macro-nutrients; North Atlantic; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species

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Abstract:

Coccolithophores, a key phytoplankton group, are one of the most studied organisms regarding their physiological response to ocean acidification/carbonation. The biogenic production of calcareous coccoliths has made coccolithophores a promising group for paleoceanographic research aiming to reconstruct past environmental conditions. Recently, geochemical and morphological analyses of fossil coccoliths have gained increased interest in regard to changes in seawater carbonate chemistry. The cosmopolitan coccolithophore Emiliania huxleyi (Lohm.) Hay and Mohler was cultured over a range of pCO2 levels in controlled laboratory experiments under nutrient replete and nitrogen limited conditions. Measurements of photosynthesis and calcification revealed, as previously published, an increase in particulate organic carbon production and a moderate decrease in calcification from ambient to elevated pCO2. The enhancement in particulate organic carbon production was accompanied by an increase in cell diameter. Changes in coccolith volume were best correlated with the coccosphere/cell diameter and no significant correlation was found between the coccolith volume and the particulate inorganic carbon production. The conducted experiments revealed that the coccolith volume of E. huxleyi is variable with aquatic CO2 concentration but its sensitivity is rather small in comparison with its sensitivity to nitrogen limitation. Comparing coccolith morphological and geometrical parameters like volume, mass and size to physiological parameters under controlled laboratory conditions is an important step to understand variations in fossil coccolith geometry.

Keyword(s):

Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Emiliania huxleyi; Gene expression (incl. proteomics); Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Macro-nutrients; North Atlantic; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species

Further details:

Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb

Project(s):

Biological Impacts of Ocean Acidification (BIOACID)

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-02-11.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Müller, Marius N
2	Experimental treatment	Exp treat		Müller, Marius N
3	Experiment	Exp		Müller, Marius N
4	Description	Description		Müller, Marius N
5	Code	Code		Müller, Marius N
6	Carbon, inorganic, dissolved	DIC	µmol/kg	Müller, Marius N
7	Standard deviation	Std dev	±	Müller, Marius N
8	Alkalinity, total	AT	µmol/kg	Müller, Marius N
9	Alkalinity, total, standard deviation	AT std dev	±	Müller, Marius N
10	Carbon dioxide, partial pressure	pCO2	µatm	Müller, Marius N
11	Carbon dioxide, partial pressure, standard deviation	pCO2 std dev	±	Müller, Marius N
12	pH	pH		Müller, Marius N		total scale
13	pH, standard deviation	pH std dev	±	Müller, Marius N		total scale
14	Calcite saturation state	Omega Cal		Müller, Marius N
15	Calcite saturation state, standard deviation	Omega Cal std dev	±	Müller, Marius N
16	Carbon dioxide	CO2	µmol/kg	Müller, Marius N
17	Carbon dioxide, standard deviation	CO2 std dev	±	Müller, Marius N
18	Bicarbonate ion	[HCO3]-	µmol/kg	Müller, Marius N
19	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Müller, Marius N
20	Carbonate ion	[CO3]2-	µmol/kg	Müller, Marius N
21	Carbonate ion, standard deviation	[CO3]2- std dev	±	Müller, Marius N
22	Cell density	Cells	#/ml	Müller, Marius N		cells/ml x 10**4
23	Cell density, standard deviation	Cells std dev	±	Müller, Marius N		cells/ml x 10**4
24	Growth rate	µ	1/day	Müller, Marius N
25	Growth rate, standard deviation	µ std dev	±	Müller, Marius N
26	Particulate inorganic carbon production per cell	PIC prod/cell	pg/#/day	Müller, Marius N
27	Particulate inorganic carbon, production, standard deviation	PIC prod std dev	±	Müller, Marius N
28	Particulate organic carbon production per cell	POC prod/cell	pg/#/day	Müller, Marius N
29	Particulate organic carbon, production, standard deviation	POC prod std dev	±	Müller, Marius N
30	-	-		Müller, Marius N		TPN prod/cell
31	-	-		Müller, Marius N		TPN prod std dev
32	Particulate organic phosphorus production per cell	POP prod/cell	pmol/#/day	Müller, Marius N
33	Standard deviation	Std dev	±	Müller, Marius N
34	Particulate inorganic carbon/particulate organic carbon ratio	PIC/POC		Müller, Marius N
35	Particulate inorganic carbon/particulate organic carbon ratio, standard deviation	PIC/POC ratio std dev	±	Müller, Marius N
36	Carbon, organic, particulate/Nitrogen, particulate ratio	POC/PN		Müller, Marius N
37	Standard deviation	Std dev	±	Müller, Marius N
38	Carbon, organic, particulate/Phosphorus, organic, particulate ratio	POC/POP		Müller, Marius N
39	Standard deviation	Std dev	±	Müller, Marius N
40	Nitrogen, total, particulate/Phosphorus, organic, particulate, ratio	TPN/POP		Müller, Marius N
41	Standard deviation	Std dev	±	Müller, Marius N
42	Coccoliths, other, diameter	Cocco diam	µm	Müller, Marius N
43	Standard deviation	Std dev	±	Müller, Marius N
44	Cell size	Cell size	µm	Müller, Marius N
45	Cell size, standard deviation	Cell size std dev	±	Müller, Marius N
46	Cell biovolume	Cell biovol	µm³	Müller, Marius N
47	Standard deviation	Std dev	±	Müller, Marius N
48	Salinity	Sal		Müller, Marius N
49	Temperature, water	Temp	°C	Müller, Marius N
50	Temperature, standard deviation	T std dev	±	Müller, Marius N
51	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
52	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
53	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
54	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
55	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
56	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
57	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
58	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
59	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

397 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Species	2 Exp treat	3 Exp	4 Description	5 Code	6 DIC [µmol/kg]	7 Std dev [±]	8 AT [µmol/kg]	9 AT std dev [±]	10 pCO2 [µatm]	11 pCO2 std dev [±]	12 pH (total scale)	13 pH std dev [±] (total scale)	14 Omega Cal	15 Omega Cal std dev [±]	16 CO2 [µmol/kg]	17 CO2 std dev [±]	18 [HCO3]- [µmol/kg]	19 [HCO3]- std dev [±]	20 [CO3]2- [µmol/kg]	21 [CO3]2- std dev [±]	22 Cells [#/ml] (cells/ml x 10**4)	23 Cells std dev [±] (cells/ml x 10**4)	24 µ [1/day]	25 µ std dev [±]	26 PIC prod/cell [pg/#/day]	27 PIC prod std dev [±]	28 POC prod/cell [pg/#/day]	29 POC prod std dev [±]	30 - (TPN prod/cell)	31 - (TPN prod std dev)	32 POP prod/cell [pmol/#/day]	33 Std dev [±]	34 PIC/POC	35 PIC/POC ratio std dev [±]	36 POC/PN	37 Std dev [±]	38 POC/POP	39 Std dev [±]	40 TPN/POP	41 Std dev [±]	42 Cocco diam [µm]	43 Std dev [±]	44 Cell size [µm]	45 Cell size std dev [±]	46 Cell biovol [µm³]	47 Std dev [±]	48 Sal	49 Temp [°C]	50 T std dev [±]	51 CSC flag	52 pH (total scale)	53 CO2 [µmol/kg]	54 pCO2water_SST_wet [µatm]	55 fCO2water_SST_wet [µatm]	56 [HCO3]- [µmol/kg]	57 [CO3]2- [µmol/kg]	58 Omega Arg	59 Omega Cal
Emiliania huxleyi (phytoplankton)	Temperature=20 degree Celsius; Continuous light with 300umol photons/m s; Salinity=38	Batch experiments, nutrient replete		B1	2098	30	2514	19	282	21	8.20	0.020	6.9	0.2	9	0.6	1792	38	297	10	5.1	1.5	1.02	0.07	15.7	1.8	11.3	1.0	1.36	0.12	0.20	0	1.41	0.27	9.68	1	142.0	5	14.8	1	5.10	0	4.18	0.15	1.50	0	38.5	20	0.6	15	8.22	8	262	261	1801	288	4.37	6.7
Emiliania huxleyi (phytoplankton)	Temperature=20 degree Celsius; Continuous light with 300umol photons/m s; Salinity=38	Batch experiments, nutrient replete		B2	2214	109	2525	123	442	15	8.04	0.010	5.3	0.3	14	0.5	1971	96	228	13	5.4	3.2	1.32	0.09	21.3	3.2	16.3	2.6	2.67	0.93	0.27	0	1.31	0.11	7.55	2	159.0	11	21.9	6	5.31	0	4.37	0.05	2.06	0	38.5	20	0.6	15	8.06	13	414	412	1980	220	3.35	5.1
Emiliania huxleyi (phytoplankton)	Temperature=20 degree Celsius; Continuous light with 300umol photons/m s; Salinity=38	Batch experiments, nutrient replete		B3	2314	56	2447	86	1077	165	7.70	0.080	2.7	0.5	34	5.2	2163	43	117	23	6.1	2.6	0.94	0.12	12.1	2.9	25.6	2.8	3.16	0.11	0.29	0	0.47	0.08	9.45	1	231.0	41	24.4	2	5.72	0	4.92	0.21	2.89	1	38.5	20	0.6	15	7.72	32	995	992	2172	110	1.67	2.6
Emiliania huxleyi (phytoplankton)	Temperature=20 degree Celsius; Continuous light with 300umol photons/m s; Salinity=38	Chemostat experiments, nitrogen limited	supply tanks	C1	2068	10	2577	4	207	6	8.31	0.010	8.4	0.1	7	0.2	1701	14	361	4																											38.5	20	0.6	15	8.33	6	191	190	1707	355	5.39	8.3
Emiliania huxleyi (phytoplankton)	Temperature=20 degree Celsius; Continuous light with 300umol photons/m s; Salinity=38	Chemostat experiments, nitrogen limited	supply tanks	C2	2260	23	2580	2	445	48	8.05	0.040	5.5	0.4	14	1.6	2010	38	236	17																											38.5	20	0.6	15	8.07	13	410	408	2016	231	3.50	5.4
Emiliania huxleyi (phytoplankton)	Temperature=20 degree Celsius; Continuous light with 300umol photons/m s; Salinity=38	Chemostat experiments, nitrogen limited	supply tanks	C3	2431	3	2582	3	1022	33	7.74	0.010	3.0	0.1	32	1.0	2268	5	130	4																											38.5	20	0.6	15	7.76	30	955	952	2275	126	1.91	2.9
Emiliania huxleyi (phytoplankton)	Temperature=20 degree Celsius; Continuous light with 300umol photons/m s; Salinity=38	Chemostat experiments, nitrogen limited	chemostat	C1	1988		2428		235		8.25		7.2		7		1673		308		6.8	0.6	0.49	0.01			4.5				0.16						73.4				3.98	0	3.68	0.03	0.51	0	38.5	20	0.6	15	8.27	7	217	217	1679	302	4.58	7.0
Emiliania huxleyi (phytoplankton)	Temperature=20 degree Celsius; Continuous light with 300umol photons/m s; Salinity=38	Chemostat experiments, nitrogen limited	chemostat	C2	2189		2441		544		7.96		4.4		17		1984		187		6.1	0.6	0.49	0.01	4.0		5.4		0.54		0.17		0.75		11.70		79.6		6.8		4.27	0	3.86	0.03	0.50	0	38.5	20	0.6	15	7.97	16	507	505	1991	182	2.76	4.2
Emiliania huxleyi (phytoplankton)	Temperature=20 degree Celsius; Continuous light with 300umol photons/m s; Salinity=38	Chemostat experiments, nitrogen limited	chemostat	C3	2368		2485		1180		7.67		2.5		37		2222		108		5.8	0.4	0.50	0.01	3.7		7.6		0.82		0.19		0.48		10.80		104.0		9.6		4.45	0	4.10	0.09	0.63	0	38.5	20	0.6	15	7.68	35	1117	1114	2229	103	1.57	2.4