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Endres, Sonja; Unger, Juliane; Wannicke, Nicola; Nausch, Monika; Voss, Maren; Engel, Anja (2013): Response of Nodularia spumigena to pCO2. PANGAEA,, Supplement to: Endres, S et al. (2013): Response of Nodularia spumigena to pCO2; Part 2: Exudation and extracellular enzyme activities. Biogeosciences, 10(1), 567-582,

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The filamentous and diazotrophic cyanobacterium Nodularia spumigena plays a major role in the productivity of the Baltic Sea as it forms extensive blooms regularly. Under phosphorus limiting conditions Nodularia spumigena has a high enzyme affinity for dissolved organic phosphorus (DOP) by production and release of alkaline phosphatase. Additionally, it is able to degrade proteinaceous compounds by expressing the extracellular enzyme leucine aminopeptidase. As atmospheric CO2 concentrations are increasing, we expect marine phytoplankton to experience changes in several environmental parameters including pH, temperature, and nutrient availability. The aim of this study was to investigate the combined effect of CO2-induced changes in seawater carbonate chemistry and of phosphate deficiency on the exudation of organic matter, and its subsequent recycling by extracellular enzymes in a Nodularia spumigena culture. Batch cultures of Nodularia spumigena were grown for 15 days aerated with three different pCO2 levels corresponding to values from glacial periods to future values projected for the year 2100. Extracellular enzyme activities as well as changes in organic and inorganic compound concentrations were monitored. CO2 treatment-related effects were identified for cyanobacterial growth, which in turn was influencing exudation and recycling of organic matter by extracellular enzymes. Biomass production was increased by 56.5% and 90.7% in the medium and high pCO2 treatment, respectively, compared to the low pCO2 treatment and simultaneously increasing exudation. During the growth phase significantly more mucinous substances accumulated in the high pCO2 treatment reaching 363 µg Gum Xanthan eq /l compared to 269 µg Gum Xanthan eq /l in the low pCO2 treatment. However, cell-specific rates did not change. After phosphate depletion, the acquisition of P from DOP by alkaline phosphatase was significantly enhanced. Alkaline phosphatase activities were increased by factor 1.64 and 2.25, respectively, in the medium and high compared to the low pCO2 treatment. In conclusion, our results suggest that Nodularia spumigena can grow faster under elevated pCO2 by enhancing the recycling of organic matter to acquire nutrients.
Nodularia spumigena; Not applicable
Further details:
Lavigne, Héloise; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4.
Date/Time Start: 2010-03-29T00:00:00 * Date/Time End: 2010-04-13T00:00:00
Values are means and standard deviations of three replicates (except one replicate bottle of the 180 ppm treatment at day 9).
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-02-11.
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1SpeciesSpeciesEndres, Sonja
2DATE/TIMEDate/TimeEndres, SonjaGeocode
3Incubation durationInc durdaysEndres, Sonja
4TreatmentTreatEndres, Sonja
5Phosphate[PO4]3-µmol/lWannicke, NicolaSpectrophotometer Hitachi U-2000
6Phosphate, standard deviationPO4 std dev±Wannicke, NicolaSpectrophotometer Hitachi U-2000
7Nitrogen, inorganic, dissolvedDINµmol/lWannicke, NicolaSpectrophotometer Hitachi U-2000
8Nitrogen, inorganic, dissolved, standard deviationDIN std dev±Wannicke, NicolaSpectrophotometer Hitachi U-2000
9Chlorophyll aChl aµg/lWannicke, NicolaFluorometry
10Chlorophyll a, standard deviationChl a std dev±Wannicke, NicolaFluorometry
11Bacteria, abundanceBact abund109 #/cm3Wannicke, NicolaFlow cytometry
12Bacteria, abundance, standard deviationBact abund std dev±Wannicke, NicolaFlow cytometry
13Carbon, organic, dissolvedDOCµmol/lEndres, SonjaTOC analyzer (Shimadzu)
14Carbon, organic, dissolved, standard deviationDOC std dev±Endres, SonjaTOC analyzer (Shimadzu)
15Nitrogen, organic, dissolvedDONµmol/lEndres, SonjaTOC analyzer (Shimadzu)
16Standard deviationStd dev±Endres, SonjaTOC analyzer (Shimadzu)
17Phosphate, organic, dissolvedDOPµmol/lUnger, JulianeSpectrophotometer (SPEC)
18Standard deviationStd dev±Unger, JulianeSpectrophotometer (SPEC)
19Mucinous substancesMucinous subsµg Xeq/lEndres, SonjaUV/VIS Spectrometer
20Standard deviationStd dev±Endres, SonjaUV/VIS Spectrometer
21Coomassie stainable particlesCSPµm2/lEndres, SonjaMicroscopy
22Standard deviationStd dev±Endres, SonjaMicroscopy
23Coomassie stainable particles, abundanceCSP abund1/lEndres, SonjaMicroscopy
24Standard deviationStd dev±Endres, SonjaMicroscopy
25Nodularia spumigenaN. spumigena#/lWannicke, NicolaMicroscopy
26Standard deviationStd dev±Wannicke, NicolaMicroscopy
27Coomassie stainable particles, equivalent spherical diameterCSP ESDµmEndres, SonjaMicroscopy
28Standard deviationStd dev±Endres, SonjaMicroscopy
29alpha-glucosidase activityMUF-agluµmol/l/hEndres, SonjaFluorometry
30Standard deviationStd dev±Endres, SonjaFluorometry
31beta-glucosidase activityMUF-bgluµmol/l/hEndres, SonjaFluorometry
32Standard deviationStd dev±Endres, SonjaFluorometry
33Leucine aminopeptidase activityLeu aminopepnmol/l/hEndres, SonjaFluorometry
34Standard deviationStd dev±Endres, SonjaFluorometry
35Alkaline phosphatase activityALKP Anmol/l/hEndres, SonjaFluorometry
36Standard deviationStd dev±Endres, SonjaFluorometry
37Alkaline phosphatase, Km valueALKP Kmµmol/lEndres, SonjaFluorometry
38Standard deviationStd dev±Endres, SonjaFluorometry
39Temperature, waterTemp°CWannicke, Nicola
40SalinitySalWannicke, Nicola
41pHpHWannicke, Nicola
42pH, standard deviationpH std dev±Wannicke, Nicola
43Carbon, inorganic, dissolvedDICµmol/kgWannicke, Nicola
44Carbon, inorganic, dissolved, standard deviationDIC std dev±Wannicke, Nicola
45Alkalinity, totalATµmol/kgWannicke, Nicolatotal scale
46Alkalinity, total, standard deviationAT std dev±Wannicke, Nicolatotal scale
47Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmWannicke, Nicola
48Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Wannicke, Nicola
49Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
50Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
51Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
52Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
53Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
54Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
55Alkalinity, total, standard deviationAT std dev±Yang, YanCalculated using seacarb after Nisumaa et al. (2010)
56Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
57Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
660 data points

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