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Van de Waal, Dedmer B; John, Uwe; Ziveri, Patrizia; Reichart, Gert-Jan; Hoins, Mirja; Sluijs, Appy; Rost, Bjoern (2013): Seawater carbonate chemistry and growth, calcification of Thoracosphaera heimii in a laboratory experiment. doi:10.1594/PANGAEA.824705, Supplement to: Van de Waal, DB et al. (2013): Ocean Acidification Reduces Growth and Calcification in a Marine Dinoflagellate. PLoS ONE, 8(6), e65987, doi:10.1371/journal.pone.0065987

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Abstract:
Ocean acidification is considered a major threat to marine ecosystems and may particularly affect calcifying organisms such as corals, foraminifera and coccolithophores. Here we investigate the impact of elevated pCO2 and lowered pH on growth and calcification in the common calcareous dinoflagellate Thoracosphaera heimii. We observe a substantial reduction in growth rate, calcification and cyst stability of T. heimii under elevated pCO2. Furthermore, transcriptomic analyses reveal CO2 sensitive regulation of many genes, particularly those being associated to inorganic carbon acquisition and calcification. Stable carbon isotope fractionation for organic carbon production increased with increasing pCO2 whereas it decreased for calcification, which suggests interdependence between both processes. We also found a strong effect of pCO2 on the stable oxygen isotopic composition of calcite, in line with earlier observations concerning another T. heimii strain. The observed changes in stable oxygen and carbon isotope composition of T. heimii cysts may provide an ideal tool for reconstructing past seawater carbonate chemistry, and ultimately past pCO2. Although the function of calcification in T. heimii remains unresolved, this trait likely plays an important role in the ecological and evolutionary success of this species. Acting on calcification as well as growth, ocean acidification may therefore impose a great threat for T. heimii.
Original version:
Van de Waal, Dedmer B; John, Uwe; Ziveri, Patrizia; Reichart, Gert-Jan; Hoins, Mirja; Sluijs, Appy; Rost, Bjoern (2013): General responses of Thoracosphaera heimii grown under a range of pCO2. doi:10.1594/PANGAEA.816429
Further details:
Lavigne, Héloise; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-12-16.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1IdentificationIDVan de Waal, Dedmer B
2SpeciesSpeciesVan de Waal, Dedmer B
3TreatmentTreatVan de Waal, Dedmer B
4ReplicatesRepl#Van de Waal, Dedmer B
5Carbon dioxideCO2µmol/lVan de Waal, Dedmer B
6Growth rateµ1/dayVan de Waal, Dedmer B
7Particulate organic carbon production per cellPOC prodpg/#Van de Waal, Dedmer B
8Particulate inorganic carbon production per cellPIC prodpg/#Van de Waal, Dedmer B
9Particulate inorganic carbon/particulate organic carbon ratioPIC/POC ratioVan de Waal, Dedmer BCalculated
10CystsCysts%Van de Waal, Dedmer Bcompleted
11Fractionation of calciteepsilon kper milVan de Waal, Dedmer B
12Fractionation of organic carbonepsilon pper milVan de Waal, Dedmer B
13delta 18O, calcited18O calper mil PDBVan de Waal, Dedmer B
14delta 18O, dissolved inorganic carbond18O DICper milVan de Waal, Dedmer B
15delta 18O, calcited18O calper mil SMOWVan de Waal, Dedmer Bcalcite-water
16Carbonate ion[CO3]2-µmol/lVan de Waal, Dedmer B
17Protein nameProteinVan de Waal, Dedmer B
18Gene expressionGene expressionVan de Waal, Dedmer Btreatment/present day
19Incubation durationInc durdaysVan de Waal, Dedmer B
20Abundance per volumeAbund v#/mlVan de Waal, Dedmer Bvegetative cysts/mL
21CategoryCatVan de Waal, Dedmer BKOG
22Gene expressionGene expressionVan de Waal, Dedmer Bdistribution
23SalinitySalVan de Waal, Dedmer B
24Temperature, waterTemp°CVan de Waal, Dedmer B
25Partial pressure of carbon dioxide (water) at equilibrator temperature (wet air)pCO2water_equ_wetµatmVan de Waal, Dedmer BCalculated using CO2SYSstart
26pHpHVan de Waal, Dedmer BPotentiometricstart, NBS scale
27Carbon, inorganic, dissolvedDICµmol/kgVan de Waal, Dedmer BCalculated using CO2SYSstart
28Carbon dioxideCO2µmol/kgVan de Waal, Dedmer BCalculated using CO2SYSstart
29Alkalinity, totalATµmol/kgVan de Waal, Dedmer BPotentiometric titrationstart
30Calcite saturation stateOmega CalVan de Waal, Dedmer BCalculated using CO2SYSstart
31Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)start
32pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)start, total scale
33Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)start
34Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)start
35Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)start
36Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)start
37Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)start
38Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)start
39Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)start
40Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)start
41Partial pressure of carbon dioxide (water) at equilibrator temperature (wet air)pCO2water_equ_wetµatmVan de Waal, Dedmer BCalculated using CO2SYSend
42pHpHVan de Waal, Dedmer BPotentiometricend, NBS scale
43Carbon, inorganic, dissolvedDICµmol/kgVan de Waal, Dedmer BCalculated using CO2SYSend
44Carbon dioxideCO2µmol/kgVan de Waal, Dedmer BCalculated using CO2SYSend
45Alkalinity, totalATµmol/kgVan de Waal, Dedmer BPotentiometric titrationend
46Calcite saturation stateOmega CalVan de Waal, Dedmer BCalculated using CO2SYSend
47Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)end
48pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)end, total scale
49Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)end
50Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)end
51Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)end
52Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)end
53Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)end
54Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)end
55Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)end
56Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)end
Size:
8222 data points

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