/* DATA DESCRIPTION: Citation: Van de Waal, Dedmer B; John, Uwe; Ziveri, Patrizia; Reichart, Gert-Jan; Hoins, Mirja; Sluijs, Appy; Rost, Björn (2013): Seawater carbonate chemistry and growth, calcification of Thoracosphaera heimii in a laboratory experiment. PANGAEA, https://doi.org/10.1594/PANGAEA.824705, Supplement to: Van de Waal, DB et al. (2013): Ocean Acidification Reduces Growth and Calcification in a Marine Dinoflagellate. PLoS ONE, 8(6), e65987, https://doi.org/10.1371/journal.pone.0065987 Abstract: Ocean acidification is considered a major threat to marine ecosystems and may particularly affect calcifying organisms such as corals, foraminifera and coccolithophores. Here we investigate the impact of elevated pCO2 and lowered pH on growth and calcification in the common calcareous dinoflagellate Thoracosphaera heimii. We observe a substantial reduction in growth rate, calcification and cyst stability of T. heimii under elevated pCO2. Furthermore, transcriptomic analyses reveal CO2 sensitive regulation of many genes, particularly those being associated to inorganic carbon acquisition and calcification. Stable carbon isotope fractionation for organic carbon production increased with increasing pCO2 whereas it decreased for calcification, which suggests interdependence between both processes. We also found a strong effect of pCO2 on the stable oxygen isotopic composition of calcite, in line with earlier observations concerning another T. heimii strain. The observed changes in stable oxygen and carbon isotope composition of T. heimii cysts may provide an ideal tool for reconstructing past seawater carbonate chemistry, and ultimately past pCO2. Although the function of calcification in T. heimii remains unresolved, this trait likely plays an important role in the ecological and evolutionary success of this species. Acting on calcification as well as growth, ocean acidification may therefore impose a great threat for T. heimii. Keyword(s): Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Myzozoa; Not applicable; Pelagos; Phytoplankton; Single species; Thoracosphaera heimii Original version: Van de Waal, Dedmer B; John, Uwe; Ziveri, Patrizia; Reichart, Gert-Jan; Hoins, Mirja; Sluijs, Appy; Rost, Björn (2013): General responses of Thoracosphaera heimii grown under a range of pCO2. PANGAEA, https://doi.org/10.1594/PANGAEA.816429 Further details: Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb Project(s): Mediterranean Sea Acidification in a Changing Climate (MedSeA) (URI: http://medsea-project.eu/) Ocean Acidification International Coordination Centre (OA-ICC) (URI: https://www.iaea.org/services/oa-icc/science-and-collaboration/data-access-and-management) Funding: Seventh Framework Programme (FP7), grant/award no. 265103: Mediterranean Sea Acidification in a Changing Climate (URI: https://cordis.europa.eu/project/id/265103) Comment: In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-12-16. Parameter(s): Identification (ID) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) Species (Species) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) Treatment (Treat) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) Replicates [#] (Repl) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) Carbon dioxide [µmol/l] (CO2) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) Growth rate [1/day] (µ) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) Particulate organic carbon production per cell [pg/#] (POC prod/cell) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) Particulate inorganic carbon production per cell [pg/#] (PIC prod/cell) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) Particulate inorganic carbon/particulate organic carbon ratio (PIC/POC) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) * METHOD/DEVICE: Calculated Cysts [%] (Cysts) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) * COMMENT: completed Fractionation of calcite [‰] (εk) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) Fractionation of organic carbon [‰] (εp Corg) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) δ18O, calcite [‰ PDB] (δ18O cal) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) δ18O, dissolved inorganic carbon [‰] (δ18O DIC) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) δ18O, calcite [‰ SMOW] (δ18O cal) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) * COMMENT: calcite-water Carbonate ion [µmol/l] ([CO3]2-) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) Protein name (Protein) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) Gene expression (Gene expression) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) * COMMENT: treatment/present day Incubation duration [days] (Inc dur) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) Abundance per volume [#/ml] (Abund v) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) * COMMENT: vegetative cysts/mL Category (Cat) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) * COMMENT: KOG Gene expression (Gene expression) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) * COMMENT: distribution Salinity (Sal) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) Temperature, water [°C] (Temp) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) Partial pressure of carbon dioxide (water) at equilibrator temperature (wet air) [µatm] (pCO2water_equ_wet) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) * METHOD/DEVICE: Calculated using CO2SYS (URI: http://cdiac.ornl.gov/oceans/co2rprt.html) * COMMENT: start pH (pH) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) * METHOD/DEVICE: Potentiometric * COMMENT: start, NBS scale Carbon, inorganic, dissolved [µmol/kg] (DIC) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) * METHOD/DEVICE: Calculated using CO2SYS (URI: http://cdiac.ornl.gov/oceans/co2rprt.html) * COMMENT: start Carbon dioxide [µmol/kg] (CO2) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) * METHOD/DEVICE: Calculated using CO2SYS (URI: http://cdiac.ornl.gov/oceans/co2rprt.html) * COMMENT: start Alkalinity, total [µmol/kg] (AT) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) * METHOD/DEVICE: Potentiometric titration * COMMENT: start Calcite saturation state (Omega Cal) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) * METHOD/DEVICE: Calculated using CO2SYS (URI: http://cdiac.ornl.gov/oceans/co2rprt.html) * COMMENT: start Carbonate system computation flag (CSC flag) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: start pH (pH) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: start, total scale Carbon dioxide [µmol/kg] (CO2) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: start Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) [µatm] (pCO2water_SST_wet) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: start Fugacity of carbon dioxide (water) at sea surface temperature (wet air) [µatm] (fCO2water_SST_wet) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: start Bicarbonate ion [µmol/kg] ([HCO3]-) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: start Carbonate ion [µmol/kg] ([CO3]2-) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: start Carbon, inorganic, dissolved [µmol/kg] (DIC) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: start Aragonite saturation state (Omega Arg) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: start Calcite saturation state (Omega Cal) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: start Partial pressure of carbon dioxide (water) at equilibrator temperature (wet air) [µatm] (pCO2water_equ_wet) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) * METHOD/DEVICE: Calculated using CO2SYS (URI: http://cdiac.ornl.gov/oceans/co2rprt.html) * COMMENT: end pH (pH) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) * METHOD/DEVICE: Potentiometric * COMMENT: end, NBS scale Carbon, inorganic, dissolved [µmol/kg] (DIC) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) * METHOD/DEVICE: Calculated using CO2SYS (URI: http://cdiac.ornl.gov/oceans/co2rprt.html) * COMMENT: end Carbon dioxide [µmol/kg] (CO2) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) * METHOD/DEVICE: Calculated using CO2SYS (URI: http://cdiac.ornl.gov/oceans/co2rprt.html) * COMMENT: end Alkalinity, total [µmol/kg] (AT) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) * METHOD/DEVICE: Potentiometric titration * COMMENT: end Calcite saturation state (Omega Cal) * PI: Van de Waal, Dedmer B (https://orcid.org/0000-0001-8803-1247, d.vandewaal@nioo.knaw.nl) * METHOD/DEVICE: Calculated using CO2SYS (URI: http://cdiac.ornl.gov/oceans/co2rprt.html) * COMMENT: end Carbonate system computation flag (CSC flag) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: end pH (pH) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: end, total scale Carbon dioxide [µmol/kg] (CO2) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: end Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) [µatm] (pCO2water_SST_wet) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: end Fugacity of carbon dioxide (water) at sea surface temperature (wet air) [µatm] (fCO2water_SST_wet) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: end Bicarbonate ion [µmol/kg] ([HCO3]-) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: end Carbonate ion [µmol/kg] ([CO3]2-) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: end Carbon, inorganic, dissolved [µmol/kg] (DIC) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: end Aragonite saturation state (Omega Arg) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: end Calcite saturation state (Omega Cal) * PI: Yang, Yan (https://orcid.org/0000-0003-4900-5606, yangyan@xmu.edu.cn, http://mel.xmu.edu.cn/en/) * METHOD/DEVICE: Calculated using seacarb after Nisumaa et al. (2010) (URI: https://doi.org/10.5194/essd-2-167-2010) * COMMENT: end License: Creative Commons Attribution 3.0 Unported (CC-BY-3.0) (URI: https://creativecommons.org/licenses/by/3.0/) Status: Curation Level: Enhanced curation (URI: https://wiki.pangaea.de/wiki/Curation_levels) Size: 8222 data points */ ID Species Treat Repl [#] CO2 [µmol/l] µ [1/day] POC prod/cell [pg/#] PIC prod/cell [pg/#] PIC/POC (Calculated) Cysts [%] (completed) εk [‰] εp Corg [‰] δ18O cal [‰ PDB] δ18O DIC [‰] δ18O cal [‰ SMOW] (calcite-water) [CO3]2- [µmol/l] Protein Gene expression (treatment/present day) Inc dur [days] Abund v [#/ml] (vegetative cysts/mL) Cat (KOG) Gene expression (distribution) Sal Temp [°C] pCO2water_equ_wet [µatm] (start, Calculated using CO2SYS) pH (start, NBS scale, Potentiometric) DIC [µmol/kg] (start, Calculated using CO2SYS) CO2 [µmol/kg] (start, Calculated using CO2SYS) AT [µmol/kg] (start, Potentiometric titration) Omega Cal (start, Calculated using CO2SYS) CSC flag (start, Calculated using seaca...) pH (start, total scale, Calculate...) CO2 [µmol/kg] (start, Calculated using seaca...) pCO2water_SST_wet [µatm] (start, Calculated using seaca...) fCO2water_SST_wet [µatm] (start, Calculated using seaca...) [HCO3]- [µmol/kg] (start, Calculated using seaca...) [CO3]2- [µmol/kg] (start, Calculated using seaca...) DIC [µmol/kg] (start, Calculated using seaca...) Omega Arg (start, Calculated using seaca...) Omega Cal (start, Calculated using seaca...) pCO2water_equ_wet [µatm] (end, Calculated using CO2SYS) pH (end, NBS scale, Potentiometric) DIC [µmol/kg] (end, Calculated using CO2SYS) CO2 [µmol/kg] (end, Calculated using CO2SYS) AT [µmol/kg] (end, Potentiometric titration) Omega Cal (end, Calculated using CO2SYS) CSC flag (end, Calculated using seacarb...) pH (end, total scale, Calculated ...) CO2 [µmol/kg] (end, Calculated using seacarb...) pCO2water_SST_wet [µatm] (end, Calculated using seacarb...) fCO2water_SST_wet [µatm] (end, Calculated using seacarb...) [HCO3]- [µmol/kg] (end, Calculated using seacarb...) [CO3]2- [µmol/kg] (end, Calculated using seacarb...) DIC [µmol/kg] (end, Calculated using seacarb...) Omega Arg (end, Calculated using seacarb...) Omega Cal (end, Calculated using seacarb...) Figure 1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 1 9 0.35 297 58 0.19 98 34 15 150 8.52 1990 5.8 2443 7.27 26 8.41 6 154 154 1683 311 2000 4.79 7.47 251 8.34 2068 9.6 2392 5.24 26 8.22 10 257 256 1843 224 2076 3.45 5.37 Figure 1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 2 9 0.42 282 77 0.27 96 34 15 150 8.52 1979 5.8 2429 7.22 26 8.41 6 154 153 1674 309 1988 4.75 7.41 238 8.35 2023 9.1 2351 5.25 26 8.24 9 244 243 1798 225 2032 3.46 5.39 Figure 1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 3 9 0.29 301 79 0.26 98 34 15 152 8.52 1977 5.8 2422 7.14 26 8.40 6 156 155 1674 306 1986 4.70 7.33 241 8.35 2031 9.3 2357 5.23 26 8.23 9 248 247 1807 224 2040 3.44 5.37 Figure 1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 1 19 0.29 316 88 0.28 97 34 15 326 8.25 2125 12.5 2396 4.43 26 8.13 13 335 334 1932 189 2134 2.91 4.54 496 8.08 2166 19.1 2354 3.18 26 7.97 19 508 507 2019 136 2174 2.09 3.27 Figure 1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 2 19 0.32 302 99 0.33 90 34 15 332 8.24 2129 12.8 2396 4.37 26 8.12 13 341 340 1938 187 2137 2.88 4.49 508 8.07 2173 19.5 2357 3.13 26 7.96 20 520 518 2027 134 2181 2.06 3.22 Figure 1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 3 19 0.31 279 89 0.32 97 34 15 332 8.24 2126 12.8 2393 4.36 26 8.12 13 340 339 1935 187 2134 2.87 4.48 513 8.08 2203 19.7 2390 3.19 26 7.96 20 526 524 2055 137 2211 2.10 3.27 Figure 1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 1 35 0.24 378 69 0.18 64 34 15 627 8.00 2251 24.1 2406 2.77 26 7.89 24 643 641 2116 118 2259 1.82 2.84 933 7.84 2287 35.9 2380 1.96 26 7.73 36 958 955 2174 84 2294 1.29 2.01 Figure 1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 2 34 0.27 320 51 0.16 66 34 15 624 8.00 2218 24.0 2371 2.70 26 7.88 24 638 636 2086 116 2226 1.78 2.78 915 7.85 2269 35.2 2364 1.97 26 7.73 35 936 933 2157 84 2276 1.30 2.02 Figure 1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 3 34 0.30 262 58 0.22 73 34 15 619 8.00 2226 23.8 2380 2.74 26 7.89 24 636 634 2093 117 2234 1.80 2.81 895 7.86 2303 34.4 2404 2.07 26 7.75 34 918 915 2187 88 2310 1.36 2.12 Figure 1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 1 64 0.14 363 36 0.10 17 34 15 1171 7.74 2284 45.0 2345 1.57 26 7.63 45 1200 1195 2179 67 2292 1.03 1.61 1711 7.59 2363 65.8 2376 1.15 26 7.48 66 1749 1743 2255 49 2370 0.76 1.18 Figure 1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 2 65 0.18 337 39 0.11 21 34 15 1224 7.73 2291 47.0 2346 1.51 26 7.61 47 1252 1247 2186 65 2298 1.00 1.56 1721 7.59 2382 66.1 2395 1.16 26 7.48 66 1765 1759 2273 50 2389 0.76 1.19 Figure 1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 3 65 0.15 327 35 0.11 23 34 15 1214 7.73 2298 46.7 2354 1.54 26 7.61 47 1247 1243 2193 65 2305 1.01 1.57 1727 7.59 2369 66.4 2381 1.15 26 7.48 66 1771 1765 2261 49 2376 0.75 1.18 Figure 3 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 1 7 8 11 -5.95 1.13 -5.35 260 34 15 150 8.52 1990 5.8 2443 7.27 26 8.41 6 154 154 1683 311 2000 4.79 7.47 251 8.34 2068 9.6 2392 5.24 26 8.22 10 257 256 1843 224 2076 3.45 5.37 Figure 3 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 2 7 10 9 -5.67 1.25 -5.15 255 34 15 150 8.52 1979 5.8 2429 7.22 26 8.41 6 154 153 1674 309 1988 4.75 7.41 238 8.35 2023 9.1 2351 5.25 26 8.24 9 244 243 1798 225 2032 3.46 5.39 Figure 3 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 3 7 12 9 -5.82 1.23 -5.31 263 34 15 152 8.52 1977 5.8 2422 7.14 26 8.40 6 156 155 1674 306 1986 4.70 7.33 241 8.35 2031 9.3 2357 5.23 26 8.23 9 248 247 1807 224 2040 3.44 5.37 Figure 3 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 1 18 11 8 -3.00 1.66 -2.32 141 34 15 326 8.25 2125 12.5 2396 4.43 26 8.13 13 335 334 1932 189 2134 2.91 4.54 496 8.08 2166 19.1 2354 3.18 26 7.97 19 508 507 2019 136 2174 2.09 3.27 Figure 3 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 2 18 12 9 -3.11 1.89 -2.65 142 34 15 332 8.24 2129 12.8 2396 4.37 26 8.12 13 341 340 1938 187 2137 2.88 4.49 508 8.07 2173 19.5 2357 3.13 26 7.96 20 520 518 2027 134 2181 2.06 3.22 Figure 3 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 3 18 12 9 -2.96 1.94 -2.56 144 34 15 332 8.24 2126 12.8 2393 4.36 26 8.12 13 340 339 1935 187 2134 2.87 4.48 513 8.08 2203 19.7 2390 3.19 26 7.96 20 526 524 2055 137 2211 2.10 3.27 Figure 3 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 1 33 12 4 -1.56 2.04 -0.98 87 34 15 627 8.00 2251 24.1 2406 2.77 26 7.89 24 643 641 2116 118 2259 1.82 2.84 933 7.84 2287 35.9 2380 1.96 26 7.73 36 958 955 2174 84 2294 1.29 2.01 Figure 3 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 2 33 14 2 -1.18 2.14 -0.70 86 34 15 624 8.00 2218 24.0 2371 2.70 26 7.88 24 638 636 2086 116 2226 1.78 2.78 915 7.85 2269 35.2 2364 1.97 26 7.73 35 936 933 2157 84 2276 1.30 2.02 Figure 3 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 3 33 15 3 -1.52 2.12 -1.02 86 34 15 619 8.00 2226 23.8 2380 2.74 26 7.89 24 636 634 2093 117 2234 1.80 2.81 895 7.86 2303 34.4 2404 2.07 26 7.75 34 918 915 2187 88 2310 1.36 2.12 Figure 3 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 1 70 17 0 -0.43 2.34 0.10 46 34 15 1171 7.74 2284 45.0 2345 1.57 26 7.63 45 1200 1195 2179 67 2292 1.03 1.61 1711 7.59 2363 65.8 2376 1.15 26 7.48 66 1749 1743 2255 49 2370 0.76 1.18 Figure 3 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 2 71 17 1 -0.49 2.37 0.02 45 34 15 1224 7.73 2291 47.0 2346 1.51 26 7.61 47 1252 1247 2186 65 2298 1.00 1.56 1721 7.59 2382 66.1 2395 1.16 26 7.48 66 1765 1759 2273 50 2389 0.76 1.19 Figure 3 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 5 72 15 0 -0.47 2.40 0.02 44 34 15 1214 7.73 2298 46.7 2354 1.54 26 7.61 47 1247 1243 2193 65 2305 1.01 1.57 1727 7.59 2369 66.4 2381 1.15 26 7.48 66 1771 1765 2261 49 2376 0.75 1.18 Figure 4 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm P-ATPase 0.06 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure 4 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm NCX 0.15 34 15 151 8.52 1980 5.8 2429 7.20 26 8.40 6 155 154 1676 308 1990 4.74 7.39 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure 4 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm VCX 0.63 34 15 151 8.52 1981 5.8 2429 7.20 26 8.40 6 155 154 1676 308 1990 4.73 7.39 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure 4 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm V-ATPase 1.09 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure 4 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm CA 0.25 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure 4 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm Aquaporin 0.67 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure 4 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm AE 0.00 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure 4 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm SAT-1 0.00 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure 4 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm P-ATPase 0.12 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure 4 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm NCX 2.31 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure 4 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm VCX 1.11 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure 4 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm V-ATPase 2.86 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure 4 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm CA 2.20 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure 4 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm Aquaporin 5.33 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure 4 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm AE 0.00 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure 4 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm SAT-1 0.00 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 1 2 129 34 15 150 8.52 1990 5.8 2443 7.27 26 8.41 6 154 154 1683 311 2000 4.79 7.47 251 8.34 2068 9.6 2392 5.24 26 8.22 10 257 256 1843 224 2076 3.45 5.37 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 1 2 122 34 15 150 8.52 1990 5.8 2443 7.27 26 8.41 6 154 154 1683 311 2000 4.79 7.47 251 8.34 2068 9.6 2392 5.24 26 8.22 10 257 256 1843 224 2076 3.45 5.37 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 1 4 239 34 15 150 8.52 1990 5.8 2443 7.27 26 8.41 6 154 154 1683 311 2000 4.79 7.47 251 8.34 2068 9.6 2392 5.24 26 8.22 10 257 256 1843 224 2076 3.45 5.37 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 1 4 280 34 15 150 8.52 1990 5.8 2443 7.27 26 8.41 6 154 154 1683 311 2000 4.79 7.47 251 8.34 2068 9.6 2392 5.24 26 8.22 10 257 256 1843 224 2076 3.45 5.37 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 1 7 515 34 15 150 8.52 1990 5.8 2443 7.27 26 8.41 6 154 154 1683 311 2000 4.79 7.47 251 8.34 2068 9.6 2392 5.24 26 8.22 10 257 256 1843 224 2076 3.45 5.37 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 1 8 905 34 15 150 8.52 1990 5.8 2443 7.27 26 8.41 6 154 154 1683 311 2000 4.79 7.47 251 8.34 2068 9.6 2392 5.24 26 8.22 10 257 256 1843 224 2076 3.45 5.37 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 1 8 950 34 15 150 8.52 1990 5.8 2443 7.27 26 8.41 6 154 154 1683 311 2000 4.79 7.47 251 8.34 2068 9.6 2392 5.24 26 8.22 10 257 256 1843 224 2076 3.45 5.37 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 1 8 940 34 15 150 8.52 1990 5.8 2443 7.27 26 8.41 6 154 154 1683 311 2000 4.79 7.47 251 8.34 2068 9.6 2392 5.24 26 8.22 10 257 256 1843 224 2076 3.45 5.37 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 2 2 98 34 15 150 8.52 1979 5.8 2429 7.22 26 8.41 6 154 153 1674 309 1988 4.75 7.41 238 8.35 2023 9.1 2351 5.25 26 8.24 9 244 243 1798 225 2032 3.46 5.39 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 2 2 94 34 15 150 8.52 1979 5.8 2429 7.22 26 8.41 6 154 153 1674 309 1988 4.75 7.41 238 8.35 2023 9.1 2351 5.25 26 8.24 9 244 243 1798 225 2032 3.46 5.39 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 2 4 189 34 15 150 8.52 1979 5.8 2429 7.22 26 8.41 6 154 153 1674 309 1988 4.75 7.41 238 8.35 2023 9.1 2351 5.25 26 8.24 9 244 243 1798 225 2032 3.46 5.39 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 2 4 245 34 15 150 8.52 1979 5.8 2429 7.22 26 8.41 6 154 153 1674 309 1988 4.75 7.41 238 8.35 2023 9.1 2351 5.25 26 8.24 9 244 243 1798 225 2032 3.46 5.39 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 2 7 585 34 15 150 8.52 1979 5.8 2429 7.22 26 8.41 6 154 153 1674 309 1988 4.75 7.41 238 8.35 2023 9.1 2351 5.25 26 8.24 9 244 243 1798 225 2032 3.46 5.39 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 2 8 855 34 15 150 8.52 1979 5.8 2429 7.22 26 8.41 6 154 153 1674 309 1988 4.75 7.41 238 8.35 2023 9.1 2351 5.25 26 8.24 9 244 243 1798 225 2032 3.46 5.39 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 2 8 1070 34 15 150 8.52 1979 5.8 2429 7.22 26 8.41 6 154 153 1674 309 1988 4.75 7.41 238 8.35 2023 9.1 2351 5.25 26 8.24 9 244 243 1798 225 2032 3.46 5.39 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 2 8 1185 34 15 150 8.52 1979 5.8 2429 7.22 26 8.41 6 154 153 1674 309 1988 4.75 7.41 238 8.35 2023 9.1 2351 5.25 26 8.24 9 244 243 1798 225 2032 3.46 5.39 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 3 2 122 34 15 152 8.52 1977 5.8 2422 7.14 26 8.40 6 156 155 1674 306 1986 4.70 7.33 241 8.35 2031 9.3 2357 5.23 26 8.23 9 248 247 1807 224 2040 3.44 5.37 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 3 2 122 34 15 152 8.52 1977 5.8 2422 7.14 26 8.40 6 156 155 1674 306 1986 4.70 7.33 241 8.35 2031 9.3 2357 5.23 26 8.23 9 248 247 1807 224 2040 3.44 5.37 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 3 4 232 34 15 152 8.52 1977 5.8 2422 7.14 26 8.40 6 156 155 1674 306 1986 4.70 7.33 241 8.35 2031 9.3 2357 5.23 26 8.23 9 248 247 1807 224 2040 3.44 5.37 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 3 4 305 34 15 152 8.52 1977 5.8 2422 7.14 26 8.40 6 156 155 1674 306 1986 4.70 7.33 241 8.35 2031 9.3 2357 5.23 26 8.23 9 248 247 1807 224 2040 3.44 5.37 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 3 7 675 34 15 152 8.52 1977 5.8 2422 7.14 26 8.40 6 156 155 1674 306 1986 4.70 7.33 241 8.35 2031 9.3 2357 5.23 26 8.23 9 248 247 1807 224 2040 3.44 5.37 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 3 8 847 34 15 152 8.52 1977 5.8 2422 7.14 26 8.40 6 156 155 1674 306 1986 4.70 7.33 241 8.35 2031 9.3 2357 5.23 26 8.23 9 248 247 1807 224 2040 3.44 5.37 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 3 8 805 34 15 152 8.52 1977 5.8 2422 7.14 26 8.40 6 156 155 1674 306 1986 4.70 7.33 241 8.35 2031 9.3 2357 5.23 26 8.23 9 248 247 1807 224 2040 3.44 5.37 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm 3 8 835 34 15 152 8.52 1977 5.8 2422 7.14 26 8.40 6 156 155 1674 306 1986 4.70 7.33 241 8.35 2031 9.3 2357 5.23 26 8.23 9 248 247 1807 224 2040 3.44 5.37 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 1 2 151 34 15 326 8.25 2125 12.5 2396 4.43 26 8.13 13 335 334 1932 189 2134 2.91 4.54 496 8.08 2166 19.1 2354 3.18 26 7.97 19 508 507 2019 136 2174 2.09 3.27 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 1 2 180 34 15 326 8.25 2125 12.5 2396 4.43 26 8.13 13 335 334 1932 189 2134 2.91 4.54 496 8.08 2166 19.1 2354 3.18 26 7.97 19 508 507 2019 136 2174 2.09 3.27 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 1 4 256 34 15 326 8.25 2125 12.5 2396 4.43 26 8.13 13 335 334 1932 189 2134 2.91 4.54 496 8.08 2166 19.1 2354 3.18 26 7.97 19 508 507 2019 136 2174 2.09 3.27 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 1 4 325 34 15 326 8.25 2125 12.5 2396 4.43 26 8.13 13 335 334 1932 189 2134 2.91 4.54 496 8.08 2166 19.1 2354 3.18 26 7.97 19 508 507 2019 136 2174 2.09 3.27 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 1 7 610 34 15 326 8.25 2125 12.5 2396 4.43 26 8.13 13 335 334 1932 189 2134 2.91 4.54 496 8.08 2166 19.1 2354 3.18 26 7.97 19 508 507 2019 136 2174 2.09 3.27 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 1 8 1010 34 15 326 8.25 2125 12.5 2396 4.43 26 8.13 13 335 334 1932 189 2134 2.91 4.54 496 8.08 2166 19.1 2354 3.18 26 7.97 19 508 507 2019 136 2174 2.09 3.27 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 1 8 875 34 15 326 8.25 2125 12.5 2396 4.43 26 8.13 13 335 334 1932 189 2134 2.91 4.54 496 8.08 2166 19.1 2354 3.18 26 7.97 19 508 507 2019 136 2174 2.09 3.27 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 1 8 880 34 15 326 8.25 2125 12.5 2396 4.43 26 8.13 13 335 334 1932 189 2134 2.91 4.54 496 8.08 2166 19.1 2354 3.18 26 7.97 19 508 507 2019 136 2174 2.09 3.27 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 2 2 146 34 15 332 8.24 2129 12.8 2396 4.37 26 8.12 13 341 340 1938 187 2137 2.88 4.49 508 8.07 2173 19.5 2357 3.13 26 7.96 20 520 518 2027 134 2181 2.06 3.22 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 2 2 105 34 15 332 8.24 2129 12.8 2396 4.37 26 8.12 13 341 340 1938 187 2137 2.88 4.49 508 8.07 2173 19.5 2357 3.13 26 7.96 20 520 518 2027 134 2181 2.06 3.22 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 2 4 242 34 15 332 8.24 2129 12.8 2396 4.37 26 8.12 13 341 340 1938 187 2137 2.88 4.49 508 8.07 2173 19.5 2357 3.13 26 7.96 20 520 518 2027 134 2181 2.06 3.22 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 2 4 250 34 15 332 8.24 2129 12.8 2396 4.37 26 8.12 13 341 340 1938 187 2137 2.88 4.49 508 8.07 2173 19.5 2357 3.13 26 7.96 20 520 518 2027 134 2181 2.06 3.22 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 2 7 790 34 15 332 8.24 2129 12.8 2396 4.37 26 8.12 13 341 340 1938 187 2137 2.88 4.49 508 8.07 2173 19.5 2357 3.13 26 7.96 20 520 518 2027 134 2181 2.06 3.22 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 2 8 1040 34 15 332 8.24 2129 12.8 2396 4.37 26 8.12 13 341 340 1938 187 2137 2.88 4.49 508 8.07 2173 19.5 2357 3.13 26 7.96 20 520 518 2027 134 2181 2.06 3.22 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 2 8 910 34 15 332 8.24 2129 12.8 2396 4.37 26 8.12 13 341 340 1938 187 2137 2.88 4.49 508 8.07 2173 19.5 2357 3.13 26 7.96 20 520 518 2027 134 2181 2.06 3.22 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 2 8 890 34 15 332 8.24 2129 12.8 2396 4.37 26 8.12 13 341 340 1938 187 2137 2.88 4.49 508 8.07 2173 19.5 2357 3.13 26 7.96 20 520 518 2027 134 2181 2.06 3.22 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 3 2 132 34 15 332 8.24 2126 12.8 2393 4.36 26 8.12 13 340 339 1935 187 2134 2.87 4.48 513 8.08 2203 19.7 2390 3.19 26 7.96 20 526 524 2055 137 2211 2.10 3.27 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 3 2 210 34 15 332 8.24 2126 12.8 2393 4.36 26 8.12 13 340 339 1935 187 2134 2.87 4.48 513 8.08 2203 19.7 2390 3.19 26 7.96 20 526 524 2055 137 2211 2.10 3.27 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 3 4 244 34 15 332 8.24 2126 12.8 2393 4.36 26 8.12 13 340 339 1935 187 2134 2.87 4.48 513 8.08 2203 19.7 2390 3.19 26 7.96 20 526 524 2055 137 2211 2.10 3.27 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 3 4 270 34 15 332 8.24 2126 12.8 2393 4.36 26 8.12 13 340 339 1935 187 2134 2.87 4.48 513 8.08 2203 19.7 2390 3.19 26 7.96 20 526 524 2055 137 2211 2.10 3.27 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 3 7 710 34 15 332 8.24 2126 12.8 2393 4.36 26 8.12 13 340 339 1935 187 2134 2.87 4.48 513 8.08 2203 19.7 2390 3.19 26 7.96 20 526 524 2055 137 2211 2.10 3.27 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 3 8 1107 34 15 332 8.24 2126 12.8 2393 4.36 26 8.12 13 340 339 1935 187 2134 2.87 4.48 513 8.08 2203 19.7 2390 3.19 26 7.96 20 526 524 2055 137 2211 2.10 3.27 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 3 8 1000 34 15 332 8.24 2126 12.8 2393 4.36 26 8.12 13 340 339 1935 187 2134 2.87 4.48 513 8.08 2203 19.7 2390 3.19 26 7.96 20 526 524 2055 137 2211 2.10 3.27 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm 3 8 770 34 15 332 8.24 2126 12.8 2393 4.36 26 8.12 13 340 339 1935 187 2134 2.87 4.48 513 8.08 2203 19.7 2390 3.19 26 7.96 20 526 524 2055 137 2211 2.10 3.27 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 1 2 170 34 15 627 8.00 2251 24.1 2406 2.77 26 7.89 24 643 641 2116 118 2259 1.82 2.84 933 7.84 2287 35.9 2380 1.96 26 7.73 36 958 955 2174 84 2294 1.29 2.01 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 1 2 210 34 15 627 8.00 2251 24.1 2406 2.77 26 7.89 24 643 641 2116 118 2259 1.82 2.84 933 7.84 2287 35.9 2380 1.96 26 7.73 36 958 955 2174 84 2294 1.29 2.01 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 1 4 324 34 15 627 8.00 2251 24.1 2406 2.77 26 7.89 24 643 641 2116 118 2259 1.82 2.84 933 7.84 2287 35.9 2380 1.96 26 7.73 36 958 955 2174 84 2294 1.29 2.01 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 1 4 285 34 15 627 8.00 2251 24.1 2406 2.77 26 7.89 24 643 641 2116 118 2259 1.82 2.84 933 7.84 2287 35.9 2380 1.96 26 7.73 36 958 955 2174 84 2294 1.29 2.01 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 1 7 925 34 15 627 8.00 2251 24.1 2406 2.77 26 7.89 24 643 641 2116 118 2259 1.82 2.84 933 7.84 2287 35.9 2380 1.96 26 7.73 36 958 955 2174 84 2294 1.29 2.01 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 1 8 34 15 627 8.00 2251 24.1 2406 2.77 26 7.89 24 643 641 2116 118 2259 1.82 2.84 933 7.84 2287 35.9 2380 1.96 26 7.73 36 958 955 2174 84 2294 1.29 2.01 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 1 8 765 34 15 627 8.00 2251 24.1 2406 2.77 26 7.89 24 643 641 2116 118 2259 1.82 2.84 933 7.84 2287 35.9 2380 1.96 26 7.73 36 958 955 2174 84 2294 1.29 2.01 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 1 8 845 34 15 627 8.00 2251 24.1 2406 2.77 26 7.89 24 643 641 2116 118 2259 1.82 2.84 933 7.84 2287 35.9 2380 1.96 26 7.73 36 958 955 2174 84 2294 1.29 2.01 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 2 2 153 34 15 624 8.00 2218 24.0 2371 2.70 26 7.88 24 638 636 2086 116 2226 1.78 2.78 915 7.85 2269 35.2 2364 1.97 26 7.73 35 936 933 2157 84 2276 1.30 2.02 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 2 2 210 34 15 624 8.00 2218 24.0 2371 2.70 26 7.88 24 638 636 2086 116 2226 1.78 2.78 915 7.85 2269 35.2 2364 1.97 26 7.73 35 936 933 2157 84 2276 1.30 2.02 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 2 4 286 34 15 624 8.00 2218 24.0 2371 2.70 26 7.88 24 638 636 2086 116 2226 1.78 2.78 915 7.85 2269 35.2 2364 1.97 26 7.73 35 936 933 2157 84 2276 1.30 2.02 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 2 4 375 34 15 624 8.00 2218 24.0 2371 2.70 26 7.88 24 638 636 2086 116 2226 1.78 2.78 915 7.85 2269 35.2 2364 1.97 26 7.73 35 936 933 2157 84 2276 1.30 2.02 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 2 7 850 34 15 624 8.00 2218 24.0 2371 2.70 26 7.88 24 638 636 2086 116 2226 1.78 2.78 915 7.85 2269 35.2 2364 1.97 26 7.73 35 936 933 2157 84 2276 1.30 2.02 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 2 8 907 34 15 624 8.00 2218 24.0 2371 2.70 26 7.88 24 638 636 2086 116 2226 1.78 2.78 915 7.85 2269 35.2 2364 1.97 26 7.73 35 936 933 2157 84 2276 1.30 2.02 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 2 8 985 34 15 624 8.00 2218 24.0 2371 2.70 26 7.88 24 638 636 2086 116 2226 1.78 2.78 915 7.85 2269 35.2 2364 1.97 26 7.73 35 936 933 2157 84 2276 1.30 2.02 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 2 8 1115 34 15 624 8.00 2218 24.0 2371 2.70 26 7.88 24 638 636 2086 116 2226 1.78 2.78 915 7.85 2269 35.2 2364 1.97 26 7.73 35 936 933 2157 84 2276 1.30 2.02 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 3 2 159 34 15 619 8.00 2226 23.8 2380 2.74 26 7.89 24 636 634 2093 117 2234 1.80 2.81 895 7.86 2303 34.4 2404 2.07 26 7.75 34 918 915 2187 88 2310 1.36 2.12 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 3 2 220 34 15 619 8.00 2226 23.8 2380 2.74 26 7.89 24 636 634 2093 117 2234 1.80 2.81 895 7.86 2303 34.4 2404 2.07 26 7.75 34 918 915 2187 88 2310 1.36 2.12 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 3 4 306 34 15 619 8.00 2226 23.8 2380 2.74 26 7.89 24 636 634 2093 117 2234 1.80 2.81 895 7.86 2303 34.4 2404 2.07 26 7.75 34 918 915 2187 88 2310 1.36 2.12 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 3 4 375 34 15 619 8.00 2226 23.8 2380 2.74 26 7.89 24 636 634 2093 117 2234 1.80 2.81 895 7.86 2303 34.4 2404 2.07 26 7.75 34 918 915 2187 88 2310 1.36 2.12 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 3 7 815 34 15 619 8.00 2226 23.8 2380 2.74 26 7.89 24 636 634 2093 117 2234 1.80 2.81 895 7.86 2303 34.4 2404 2.07 26 7.75 34 918 915 2187 88 2310 1.36 2.12 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 3 8 1107 34 15 619 8.00 2226 23.8 2380 2.74 26 7.89 24 636 634 2093 117 2234 1.80 2.81 895 7.86 2303 34.4 2404 2.07 26 7.75 34 918 915 2187 88 2310 1.36 2.12 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 3 8 1110 34 15 619 8.00 2226 23.8 2380 2.74 26 7.89 24 636 634 2093 117 2234 1.80 2.81 895 7.86 2303 34.4 2404 2.07 26 7.75 34 918 915 2187 88 2310 1.36 2.12 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=750 micro atm 3 8 1165 34 15 619 8.00 2226 23.8 2380 2.74 26 7.89 24 636 634 2093 117 2234 1.80 2.81 895 7.86 2303 34.4 2404 2.07 26 7.75 34 918 915 2187 88 2310 1.36 2.12 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 1 2 251 34 15 1171 7.74 2284 45.0 2345 1.57 26 7.63 45 1200 1195 2179 67 2292 1.03 1.61 1711 7.59 2363 65.8 2376 1.15 26 7.48 66 1749 1743 2255 49 2370 0.76 1.18 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 1 2 265 34 15 1171 7.74 2284 45.0 2345 1.57 26 7.63 45 1200 1195 2179 67 2292 1.03 1.61 1711 7.59 2363 65.8 2376 1.15 26 7.48 66 1749 1743 2255 49 2370 0.76 1.18 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 1 4 394 34 15 1171 7.74 2284 45.0 2345 1.57 26 7.63 45 1200 1195 2179 67 2292 1.03 1.61 1711 7.59 2363 65.8 2376 1.15 26 7.48 66 1749 1743 2255 49 2370 0.76 1.18 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 1 4 375 34 15 1171 7.74 2284 45.0 2345 1.57 26 7.63 45 1200 1195 2179 67 2292 1.03 1.61 1711 7.59 2363 65.8 2376 1.15 26 7.48 66 1749 1743 2255 49 2370 0.76 1.18 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 1 7 580 34 15 1171 7.74 2284 45.0 2345 1.57 26 7.63 45 1200 1195 2179 67 2292 1.03 1.61 1711 7.59 2363 65.8 2376 1.15 26 7.48 66 1749 1743 2255 49 2370 0.76 1.18 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 1 8 565 34 15 1171 7.74 2284 45.0 2345 1.57 26 7.63 45 1200 1195 2179 67 2292 1.03 1.61 1711 7.59 2363 65.8 2376 1.15 26 7.48 66 1749 1743 2255 49 2370 0.76 1.18 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 1 8 660 34 15 1171 7.74 2284 45.0 2345 1.57 26 7.63 45 1200 1195 2179 67 2292 1.03 1.61 1711 7.59 2363 65.8 2376 1.15 26 7.48 66 1749 1743 2255 49 2370 0.76 1.18 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 1 8 710 34 15 1171 7.74 2284 45.0 2345 1.57 26 7.63 45 1200 1195 2179 67 2292 1.03 1.61 1711 7.59 2363 65.8 2376 1.15 26 7.48 66 1749 1743 2255 49 2370 0.76 1.18 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 2 2 223 34 15 1224 7.73 2291 47.0 2346 1.51 26 7.61 47 1252 1247 2186 65 2298 1.00 1.56 1721 7.59 2382 66.1 2395 1.16 26 7.48 66 1765 1759 2273 50 2389 0.76 1.19 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 2 2 250 34 15 1224 7.73 2291 47.0 2346 1.51 26 7.61 47 1252 1247 2186 65 2298 1.00 1.56 1721 7.59 2382 66.1 2395 1.16 26 7.48 66 1765 1759 2273 50 2389 0.76 1.19 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 2 4 334 34 15 1224 7.73 2291 47.0 2346 1.51 26 7.61 47 1252 1247 2186 65 2298 1.00 1.56 1721 7.59 2382 66.1 2395 1.16 26 7.48 66 1765 1759 2273 50 2389 0.76 1.19 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 2 4 440 34 15 1224 7.73 2291 47.0 2346 1.51 26 7.61 47 1252 1247 2186 65 2298 1.00 1.56 1721 7.59 2382 66.1 2395 1.16 26 7.48 66 1765 1759 2273 50 2389 0.76 1.19 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 2 7 595 34 15 1224 7.73 2291 47.0 2346 1.51 26 7.61 47 1252 1247 2186 65 2298 1.00 1.56 1721 7.59 2382 66.1 2395 1.16 26 7.48 66 1765 1759 2273 50 2389 0.76 1.19 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 2 8 720 34 15 1224 7.73 2291 47.0 2346 1.51 26 7.61 47 1252 1247 2186 65 2298 1.00 1.56 1721 7.59 2382 66.1 2395 1.16 26 7.48 66 1765 1759 2273 50 2389 0.76 1.19 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 2 8 745 34 15 1224 7.73 2291 47.0 2346 1.51 26 7.61 47 1252 1247 2186 65 2298 1.00 1.56 1721 7.59 2382 66.1 2395 1.16 26 7.48 66 1765 1759 2273 50 2389 0.76 1.19 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 2 8 745 34 15 1224 7.73 2291 47.0 2346 1.51 26 7.61 47 1252 1247 2186 65 2298 1.00 1.56 1721 7.59 2382 66.1 2395 1.16 26 7.48 66 1765 1759 2273 50 2389 0.76 1.19 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 3 2 255 34 15 1214 7.73 2298 46.7 2354 1.54 26 7.61 47 1247 1243 2193 65 2305 1.01 1.57 1727 7.59 2369 66.4 2381 1.15 26 7.48 66 1771 1765 2261 49 2376 0.75 1.18 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 3 2 275 34 15 1214 7.73 2298 46.7 2354 1.54 26 7.61 47 1247 1243 2193 65 2305 1.01 1.57 1727 7.59 2369 66.4 2381 1.15 26 7.48 66 1771 1765 2261 49 2376 0.75 1.18 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 3 4 352 34 15 1214 7.73 2298 46.7 2354 1.54 26 7.61 47 1247 1243 2193 65 2305 1.01 1.57 1727 7.59 2369 66.4 2381 1.15 26 7.48 66 1771 1765 2261 49 2376 0.75 1.18 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 3 4 490 34 15 1214 7.73 2298 46.7 2354 1.54 26 7.61 47 1247 1243 2193 65 2305 1.01 1.57 1727 7.59 2369 66.4 2381 1.15 26 7.48 66 1771 1765 2261 49 2376 0.75 1.18 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 3 7 640 34 15 1214 7.73 2298 46.7 2354 1.54 26 7.61 47 1247 1243 2193 65 2305 1.01 1.57 1727 7.59 2369 66.4 2381 1.15 26 7.48 66 1771 1765 2261 49 2376 0.75 1.18 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 3 8 800 34 15 1214 7.73 2298 46.7 2354 1.54 26 7.61 47 1247 1243 2193 65 2305 1.01 1.57 1727 7.59 2369 66.4 2381 1.15 26 7.48 66 1771 1765 2261 49 2376 0.75 1.18 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 3 8 710 34 15 1214 7.73 2298 46.7 2354 1.54 26 7.61 47 1247 1243 2193 65 2305 1.01 1.57 1727 7.59 2369 66.4 2381 1.15 26 7.48 66 1771 1765 2261 49 2376 0.75 1.18 Figure S1 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm 3 8 645 34 15 1214 7.73 2298 46.7 2354 1.54 26 7.61 47 1247 1243 2193 65 2305 1.01 1.57 1727 7.59 2369 66.4 2381 1.15 26 7.48 66 1771 1765 2261 49 2376 0.75 1.18 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm Chromatin structure and dynamics 0.8 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm Replication, recombination and repair 2.3 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm Transcription 2.3 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm RNA processing and modification 4.1 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm Translation, ribosomal structure and biogenesis 14.2 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm Posttranslational modification, protein turnover and chaperones 14.8 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm Intracellular trafficking, secretion and vesicular transport 7.9 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm Extracellular structures 0.4 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm Cytoskeleton 3.4 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm Cell motility 0.0 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm Cell wall/membrane/envelope biogenesis 0.8 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm Signal transduction mechanisms 10.7 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm Defense mechanisms 0.3 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm Nuclear structure 0.3 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm Cell cycle control, cell division and chromosome partitioning 2.3 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm Secondary metabolites biosynthesis, transport and catabolism 2.3 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm Inorganic ion transport and metabolism 3.7 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm Lipid transport and metabolism 5.1 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm Coenzyme transport and metabolism 1.8 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm Nucleotide transport and metabolism 2.4 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm Amino acid transport and metabolism 4.9 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm Carbohydrate transport and metabolism 6.8 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=150 micro atm Energy production and conversion 8.7 34 15 151 8.52 1982 5.8 2432 7.21 26 8.40 6 154 154 1677 309 1991 4.75 7.41 243 8.35 2041 9.4 2367 5.24 26 8.23 9 249 249 1816 224 2049 3.45 5.38 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm Chromatin structure and dynamics 0.9 34 15 330 8.24 2127 12.7 2395 4.39 26 8.13 13 339 338 1935 188 2135 2.89 4.50 506 8.08 2181 19.4 2367 3.17 26 7.96 19 518 516 2034 136 2189 2.09 3.25 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm Replication, recombination and repair 1.8 34 15 330 8.24 2127 12.7 2395 4.39 26 8.13 13 339 338 1935 188 2135 2.89 4.50 506 8.08 2181 19.4 2367 3.17 26 7.96 19 518 516 2034 136 2189 2.09 3.25 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm Transcription 2.7 34 15 330 8.24 2127 12.7 2395 4.39 26 8.13 13 339 338 1935 188 2135 2.89 4.50 506 8.08 2181 19.4 2367 3.17 26 7.96 19 518 516 2034 136 2189 2.09 3.25 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm RNA processing and modification 5.4 34 15 330 8.24 2127 12.7 2395 4.39 26 8.13 13 339 338 1935 188 2135 2.89 4.50 506 8.08 2181 19.4 2367 3.17 26 7.96 19 518 516 2034 136 2189 2.09 3.25 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm Translation, ribosomal structure and biogenesis 13.4 34 15 330 8.24 2127 12.7 2395 4.39 26 8.13 13 339 338 1935 188 2135 2.89 4.50 506 8.08 2181 19.4 2367 3.17 26 7.96 19 518 516 2034 136 2189 2.09 3.25 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm Posttranslational modification, protein turnover and chaperones 14.0 34 15 330 8.24 2127 12.7 2395 4.39 26 8.13 13 339 338 1935 188 2135 2.89 4.50 506 8.08 2181 19.4 2367 3.17 26 7.96 19 518 516 2034 136 2189 2.09 3.25 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm Intracellular trafficking, secretion and vesicular transport 8.5 34 15 330 8.24 2127 12.7 2395 4.39 26 8.13 13 339 338 1935 188 2135 2.89 4.50 506 8.08 2181 19.4 2367 3.17 26 7.96 19 518 516 2034 136 2189 2.09 3.25 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm Extracellular structures 0.3 34 15 330 8.24 2127 12.7 2395 4.39 26 8.13 13 339 338 1935 188 2135 2.89 4.50 506 8.08 2181 19.4 2367 3.17 26 7.96 19 518 516 2034 136 2189 2.09 3.25 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm Cytoskeleton 3.7 34 15 330 8.24 2127 12.7 2395 4.39 26 8.13 13 339 338 1935 188 2135 2.89 4.50 506 8.08 2181 19.4 2367 3.17 26 7.96 19 518 516 2034 136 2189 2.09 3.25 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm Cell motility 0.4 34 15 330 8.24 2127 12.7 2395 4.39 26 8.13 13 339 338 1935 188 2135 2.89 4.50 506 8.08 2181 19.4 2367 3.17 26 7.96 19 518 516 2034 136 2189 2.09 3.25 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm Cell wall/membrane/envelope biogenesis 1.0 34 15 330 8.24 2127 12.7 2395 4.39 26 8.13 13 339 338 1935 188 2135 2.89 4.50 506 8.08 2181 19.4 2367 3.17 26 7.96 19 518 516 2034 136 2189 2.09 3.25 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm Signal transduction mechanisms 9.5 34 15 330 8.24 2127 12.7 2395 4.39 26 8.13 13 339 338 1935 188 2135 2.89 4.50 506 8.08 2181 19.4 2367 3.17 26 7.96 19 518 516 2034 136 2189 2.09 3.25 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm Defense mechanisms 0.5 34 15 330 8.24 2127 12.7 2395 4.39 26 8.13 13 339 338 1935 188 2135 2.89 4.50 506 8.08 2181 19.4 2367 3.17 26 7.96 19 518 516 2034 136 2189 2.09 3.25 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm Nuclear structure 0.3 34 15 330 8.24 2127 12.7 2395 4.39 26 8.13 13 339 338 1935 188 2135 2.89 4.50 506 8.08 2181 19.4 2367 3.17 26 7.96 19 518 516 2034 136 2189 2.09 3.25 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm Cell cycle control, cell division and chromosome partitioning 2.6 34 15 330 8.24 2127 12.7 2395 4.39 26 8.13 13 339 338 1935 188 2135 2.89 4.50 506 8.08 2181 19.4 2367 3.17 26 7.96 19 518 516 2034 136 2189 2.09 3.25 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm Secondary metabolites biosynthesis, transport and catabolism 2.0 34 15 330 8.24 2127 12.7 2395 4.39 26 8.13 13 339 338 1935 188 2135 2.89 4.50 506 8.08 2181 19.4 2367 3.17 26 7.96 19 518 516 2034 136 2189 2.09 3.25 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm Inorganic ion transport and metabolism 3.6 34 15 330 8.24 2127 12.7 2395 4.39 26 8.13 13 339 338 1935 188 2135 2.89 4.50 506 8.08 2181 19.4 2367 3.17 26 7.96 19 518 516 2034 136 2189 2.09 3.25 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm Lipid transport and metabolism 4.4 34 15 330 8.24 2127 12.7 2395 4.39 26 8.13 13 339 338 1935 188 2135 2.89 4.50 506 8.08 2181 19.4 2367 3.17 26 7.96 19 518 516 2034 136 2189 2.09 3.25 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm Coenzyme transport and metabolism 2.0 34 15 330 8.24 2127 12.7 2395 4.39 26 8.13 13 339 338 1935 188 2135 2.89 4.50 506 8.08 2181 19.4 2367 3.17 26 7.96 19 518 516 2034 136 2189 2.09 3.25 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm Nucleotide transport and metabolism 2.7 34 15 330 8.24 2127 12.7 2395 4.39 26 8.13 13 339 338 1935 188 2135 2.89 4.50 506 8.08 2181 19.4 2367 3.17 26 7.96 19 518 516 2034 136 2189 2.09 3.25 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm Amino acid transport and metabolism 5.0 34 15 330 8.24 2127 12.7 2395 4.39 26 8.13 13 339 338 1935 188 2135 2.89 4.50 506 8.08 2181 19.4 2367 3.17 26 7.96 19 518 516 2034 136 2189 2.09 3.25 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm Carbohydrate transport and metabolism 6.4 34 15 330 8.24 2127 12.7 2395 4.39 26 8.13 13 339 338 1935 188 2135 2.89 4.50 506 8.08 2181 19.4 2367 3.17 26 7.96 19 518 516 2034 136 2189 2.09 3.25 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=380 micro atm Energy production and conversion 9.1 34 15 330 8.24 2127 12.7 2395 4.39 26 8.13 13 339 338 1935 188 2135 2.89 4.50 506 8.08 2181 19.4 2367 3.17 26 7.96 19 518 516 2034 136 2189 2.09 3.25 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm Chromatin structure and dynamics 0.6 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm Replication, recombination and repair 1.9 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm Transcription 1.9 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm RNA processing and modification 6.5 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm Translation, ribosomal structure and biogenesis 11.9 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm Posttranslational modification, protein turnover and chaperones 15.4 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm Intracellular trafficking, secretion and vesicular transport 7.8 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm Extracellular structures 0.4 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm Cytoskeleton 3.7 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm Cell motility 0.0 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm Cell wall/membrane/envelope biogenesis 0.6 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm Signal transduction mechanisms 11.9 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm Defense mechanisms 1.1 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm Nuclear structure 0.4 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm Cell cycle control, cell division and chromosome partitioning 2.6 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm Secondary metabolites biosynthesis, transport and catabolism 1.7 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm Inorganic ion transport and metabolism 2.2 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm Lipid transport and metabolism 3.7 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm Coenzyme transport and metabolism 1.5 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm Nucleotide transport and metabolism 2.4 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm Amino acid transport and metabolism 5.0 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm Carbohydrate transport and metabolism 6.3 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18 Figure S3 Thoracosphaera heimii (phytoplankton) pCO2=1400 micro atm Energy production and conversion 10.4 34 15 1203 7.73 2291 46.2 2348 1.54 26 7.62 46 1235 1231 2186 66 2298 1.01 1.58 1719 7.59 2371 66.1 2384 1.15 26 7.48 66 1762 1756 2263 49 2378 0.76 1.18