Citation:

j.1529-8817.2012.01171.x

Name: Seawater carbonate chemistry and the photophysiology of Thalassiosira pseudonana (Bacillariophyceae) and Emiliania huxleyi (Haptophyta) in a laboratory experiment
Published: 2012
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Bottles or small containers/Aquaria (<20 L); Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; North Atlantic; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Thalassiosira pseudonana

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Abstract:

Increasing anthropogenic carbon dioxide is causing changes to ocean chemistry, which will continue in a predictable manner. Dissolution of additional atmospheric carbon dioxide leads to increased concentrations of dissolved carbon dioxide and bicarbonate and decreased pH in ocean water. The concomitant effects on phytoplankton ecophysiology, leading potentially to changes in community structure, are now a focus of concern. Therefore, we grew the coccolithophore Emiliania huxleyi (Lohmann) W. W. Hay et H. Mohler and the diatom strains Thalassiosira pseudonana (Hust.) Hasle et Heimdal CCMP 1014 and T. pseudonana CCMP 1335 under low light in turbidostat photobioreactors bubbled with air containing 390 ppmv or 750 ppmv CO2. Increased pCO2 led to increased growth rates in all three strains. In addition, protein levels of RUBISCO increased in the coastal strains of both species, showing a larger capacity for CO2 assimilation at 750 ppmv CO2. With increased pCO2, both T. pseudonana strains displayed an increased susceptibility to PSII photoinactivation and, to compensate, an augmented capacity for PSII repair. Consequently, the cost of maintaining PSII function for the diatoms increased at increased pCO2. In E. huxleyi, PSII photoinactivation and the counter-acting repair, while both intrinsically larger than in T. pseudonana, did not change between the current and high-pCO2 treatments. The content of the photosynthetic electron transport intermediary cytochrome b6/f complex increased significantly in the diatoms under elevated pCO2, suggesting changes in electron transport function.

Keyword(s):

Bottles or small containers/Aquaria (<20 L); Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; North Atlantic; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Thalassiosira pseudonana

Further details:

Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-12-16.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Identification	ID		Campbell, Douglas A
2	Species	Species		Campbell, Douglas A
3	Strain	Strain		Campbell, Douglas A
4	Treatment	Treat		Campbell, Douglas A
5	Replicates	Repl	#	Campbell, Douglas A
6	Irradiance	E	µmol/m²/s	Campbell, Douglas A		growth light
7	Irradiance	E	µmol/m²/s	Campbell, Douglas A		high light shift
8	Time in minutes	Time	min	Campbell, Douglas A		light shift duration
9	Cell density	Cells	#/ml	Campbell, Douglas A
10	Cell biovolume	Cell biovol	µm³	Campbell, Douglas A
11	Growth rate	µ	1/day	Campbell, Douglas A
12	Effective absorbance cross-section of photosystem II	sigma PSII	A²/quanta	Campbell, Douglas A		measured in the dark
13	Effective absorbance cross-section of photosystem II	sigma PSII	A²/quanta	Campbell, Douglas A		measured at growth light
14	Effective absorbance cross-section of photosystem II	sigma PSII	A²/quanta	Campbell, Douglas A		measured at light shift intensity
15	Photochemical quenching	qP		Campbell, Douglas A
16	Non photochemical quenching	NPQ		Campbell, Douglas A		releaxed by dark incubation
17	Protein per cell	Protein/cell	pg/#	Campbell, Douglas A
18	Photosynthetic protein PsbA	PsbA	pmol/µg	Campbell, Douglas A
19	Photosynthetic protein PsbD	PsbD	pmol/µg	Campbell, Douglas A
20	Photosynthetic protein Rubisco	RbcL	pmol/mg	Campbell, Douglas A
21	Photosynthetic protein, PsbC	PsaC	pmol/µg	Campbell, Douglas A
22	CF1 subunit of ATP synthase protein	AtpB	pmol/mg	Campbell, Douglas A
23	Cytochrome c1	PetC	pmol/µg	Campbell, Douglas A
24	Chlorophyll a	Chl a	pmol/l	Campbell, Douglas A
25	Chlorophyll c1/chlorophyll a ratio	Chl c1/Chl a		Campbell, Douglas A
26	Chlorophyll c2/chlorophyll a ratio	Chl c2/Chl a		Campbell, Douglas A
27	Chlorophyll c3/chlorophyll a ratio	Chl c3/Chl a		Campbell, Douglas A
28	Fucoxanthin/chlorophyll a ratio	Fuco/Chl a		Campbell, Douglas A
29	Diatoxanthin/chlorophyll a ratio	Diato/Chl a		Campbell, Douglas A
30	Diadinoxanthin/chlorophyll a ratio	Diadino/Chl a		Campbell, Douglas A
31	Carbon, organic, particulate, per cell	POC/cell	pg/#	Campbell, Douglas A
32	Carbon/Nitrogen ratio	C/N		Campbell, Douglas A
33	Temperature, water	Temp	°C	Campbell, Douglas A
34	Salinity	Sal		Campbell, Douglas A
35	Carbon, inorganic, dissolved	DIC	µmol/kg	Campbell, Douglas A
36	pH	pH		Campbell, Douglas A	Potentiometric	NBS scale
37	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	ppmv	Campbell, Douglas A	Calculated using CO2SYS
38	Alkalinity, total	AT	µmol/kg	Campbell, Douglas A	Calculated using CO2SYS
39	Bicarbonate ion	[HCO3]-	µmol/kg	Campbell, Douglas A	Calculated using CO2SYS
40	Carbonate ion	[CO3]2-	µmol/kg	Campbell, Douglas A	Calculated using CO2SYS
41	Carbon dioxide	CO2	µmol/kg	Campbell, Douglas A	Calculated using CO2SYS
42	Phosphate	[PO4]3-	µmol/l	Campbell, Douglas A
43	Silicate	Si(OH)4	µmol/l	Campbell, Douglas A
44	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
46	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
50	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
51	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
52	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
53	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1086 data points

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