Citation:

j.1529-8817.2012.01171.x

Name: Seawater carbonate chemistry and the photophysiology of Thalassiosira pseudonana (Bacillariophyceae) and Emiliania huxleyi (Haptophyta) in a laboratory experiment
Published: 2012
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Bottles or small containers/Aquaria (<20 L); Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; North Atlantic; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Thalassiosira pseudonana

Always quote citation above when using data! You can download the citation in several formats below.

RIS Citation BibTeX Citation Text Citation Facebook Twitter

Abstract:

Increasing anthropogenic carbon dioxide is causing changes to ocean chemistry, which will continue in a predictable manner. Dissolution of additional atmospheric carbon dioxide leads to increased concentrations of dissolved carbon dioxide and bicarbonate and decreased pH in ocean water. The concomitant effects on phytoplankton ecophysiology, leading potentially to changes in community structure, are now a focus of concern. Therefore, we grew the coccolithophore Emiliania huxleyi (Lohmann) W. W. Hay et H. Mohler and the diatom strains Thalassiosira pseudonana (Hust.) Hasle et Heimdal CCMP 1014 and T. pseudonana CCMP 1335 under low light in turbidostat photobioreactors bubbled with air containing 390 ppmv or 750 ppmv CO2. Increased pCO2 led to increased growth rates in all three strains. In addition, protein levels of RUBISCO increased in the coastal strains of both species, showing a larger capacity for CO2 assimilation at 750 ppmv CO2. With increased pCO2, both T. pseudonana strains displayed an increased susceptibility to PSII photoinactivation and, to compensate, an augmented capacity for PSII repair. Consequently, the cost of maintaining PSII function for the diatoms increased at increased pCO2. In E. huxleyi, PSII photoinactivation and the counter-acting repair, while both intrinsically larger than in T. pseudonana, did not change between the current and high-pCO2 treatments. The content of the photosynthetic electron transport intermediary cytochrome b6/f complex increased significantly in the diatoms under elevated pCO2, suggesting changes in electron transport function.

Keyword(s):

Bottles or small containers/Aquaria (<20 L); Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; North Atlantic; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Thalassiosira pseudonana

Further details:

Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-12-16.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Identification	ID		Campbell, Douglas A
2	Species	Species		Campbell, Douglas A
3	Strain	Strain		Campbell, Douglas A
4	Treatment	Treat		Campbell, Douglas A
5	Replicates	Repl	#	Campbell, Douglas A
6	Irradiance	E	µmol/m²/s	Campbell, Douglas A		growth light
7	Irradiance	E	µmol/m²/s	Campbell, Douglas A		high light shift
8	Time in minutes	Time	min	Campbell, Douglas A		light shift duration
9	Cell density	Cells	#/ml	Campbell, Douglas A
10	Cell biovolume	Cell biovol	µm³	Campbell, Douglas A
11	Growth rate	µ	1/day	Campbell, Douglas A
12	Effective absorbance cross-section of photosystem II	sigma PSII	A²/quanta	Campbell, Douglas A		measured in the dark
13	Effective absorbance cross-section of photosystem II	sigma PSII	A²/quanta	Campbell, Douglas A		measured at growth light
14	Effective absorbance cross-section of photosystem II	sigma PSII	A²/quanta	Campbell, Douglas A		measured at light shift intensity
15	Photochemical quenching	qP		Campbell, Douglas A
16	Non photochemical quenching	NPQ		Campbell, Douglas A		releaxed by dark incubation
17	Protein per cell	Protein/cell	pg/#	Campbell, Douglas A
18	Photosynthetic protein PsbA	PsbA	pmol/µg	Campbell, Douglas A
19	Photosynthetic protein PsbD	PsbD	pmol/µg	Campbell, Douglas A
20	Photosynthetic protein Rubisco	RbcL	pmol/mg	Campbell, Douglas A
21	Photosynthetic protein, PsbC	PsaC	pmol/µg	Campbell, Douglas A
22	CF1 subunit of ATP synthase protein	AtpB	pmol/mg	Campbell, Douglas A
23	Cytochrome c1	PetC	pmol/µg	Campbell, Douglas A
24	Chlorophyll a	Chl a	pmol/l	Campbell, Douglas A
25	Chlorophyll c1/chlorophyll a ratio	Chl c1/Chl a		Campbell, Douglas A
26	Chlorophyll c2/chlorophyll a ratio	Chl c2/Chl a		Campbell, Douglas A
27	Chlorophyll c3/chlorophyll a ratio	Chl c3/Chl a		Campbell, Douglas A
28	Fucoxanthin/chlorophyll a ratio	Fuco/Chl a		Campbell, Douglas A
29	Diatoxanthin/chlorophyll a ratio	Diato/Chl a		Campbell, Douglas A
30	Diadinoxanthin/chlorophyll a ratio	Diadino/Chl a		Campbell, Douglas A
31	Carbon, organic, particulate, per cell	POC/cell	pg/#	Campbell, Douglas A
32	Carbon/Nitrogen ratio	C/N		Campbell, Douglas A
33	Temperature, water	Temp	°C	Campbell, Douglas A
34	Salinity	Sal		Campbell, Douglas A
35	Carbon, inorganic, dissolved	DIC	µmol/kg	Campbell, Douglas A
36	pH	pH		Campbell, Douglas A	Potentiometric	NBS scale
37	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	ppmv	Campbell, Douglas A	Calculated using CO2SYS
38	Alkalinity, total	AT	µmol/kg	Campbell, Douglas A	Calculated using CO2SYS
39	Bicarbonate ion	[HCO3]-	µmol/kg	Campbell, Douglas A	Calculated using CO2SYS
40	Carbonate ion	[CO3]2-	µmol/kg	Campbell, Douglas A	Calculated using CO2SYS
41	Carbon dioxide	CO2	µmol/kg	Campbell, Douglas A	Calculated using CO2SYS
42	Phosphate	[PO4]3-	µmol/l	Campbell, Douglas A
43	Silicate	Si(OH)4	µmol/l	Campbell, Douglas A
44	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
46	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
50	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
51	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
52	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
53	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1086 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 ID	2 Species	3 Strain	4 Treat	5 Repl [#]	6 E [µmol/m²/s] (growth light)	7 E [µmol/m²/s] (high light shift)	8 Time [min] (light shift duration)	9 Cells [#/ml]	10 Cell biovol [µm³]	11 µ [1/day]	12 sigma PSII [A²/quanta] (measured in the dark)	13 sigma PSII [A²/quanta] (measured at growth light)	14 sigma PSII [A²/quanta] (measured at light shift inten...)	15 qP	16 NPQ (releaxed by dark incubation)	17 Protein/cell [pg/#]	18 PsbA [pmol/µg]	19 PsbD [pmol/µg]	20 RbcL [pmol/mg]	21 PsaC [pmol/µg]	22 AtpB [pmol/mg]	23 PetC [pmol/µg]	24 Chl a [pmol/l]	25 Chl c1/Chl a	26 Chl c2/Chl a	27 Chl c3/Chl a	28 Fuco/Chl a	29 Diato/Chl a	30 Diadino/Chl a	31 POC/cell [pg/#]	32 C/N	33 Temp [°C]	34 Sal	35 DIC [µmol/kg]	36 pH (NBS scale, Potentiometric)	37 pCO2water_SST_wet [ppmv] (Calculated using CO2SYS)	38 AT [µmol/kg] (Calculated using CO2SYS)	39 [HCO3]- [µmol/kg] (Calculated using CO2SYS)	40 [CO3]2- [µmol/kg] (Calculated using CO2SYS)	41 CO2 [µmol/kg] (Calculated using CO2SYS)	42 [PO4]3- [µmol/l]	43 Si(OH)4 [µmol/l]	44 CSC flag (Calculated using seacarb afte...)	45 pH (total scale, Calculated using...)	46 CO2 [µmol/kg] (Calculated using seacarb afte...)	47 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	48 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	49 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	50 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	51 AT [µmol/kg] (Calculated using seacarb afte...)	52 Omega Arg (Calculated using seacarb afte...)	53 Omega Cal (Calculated using seacarb afte...)
17	Thalassiosira pseudonana (phytoplankton)	CCMP 1014	pCO2=390 ppm	1	30	450	90	680000	17.7	0.581	295	248	-0.000029	0.600	0.991	1.470	0.055	0.143	1.660	0.155	0.162	0.072	0.064	0.018	0.028		0.300	0.000	0.045	3.58	6.00	18	35	1943	8.22	341	2250	1754	178	12	21	53	27	8.10	11	330	329	1748	183	2230	2.83	4.38
17	Thalassiosira pseudonana (phytoplankton)	CCMP 1014	pCO2=390 ppm	2	30	450	90	499000	17.7	0.528	299	295	-0.000020	0.622	1.034	3.410	0.045	0.108	1.459	0.120	0.147	0.069	0.071	0.018	0.028		0.307	0.001	0.049	6.25	5.52	18	35	1975	8.14	416	2243	1806	155	14	21	53	27	8.02	14	410	408	1804	157	2220	2.43	3.76
17	Thalassiosira pseudonana (phytoplankton)	CCMP 1014	pCO2=390 ppm	3	30	450	90	498000	14.3	0.360	280		-0.000026			1.800	0.050	0.086	2.083	0.043	0.058	0.088	0.039	0.009	0.038		0.324	0.000	0.044	4.08	5.94	18	35	2001	8.19	377	2296	1816	172	13	21	53	27	8.07	13	367	366	1811	177	2276	2.74	4.23
18	Thalassiosira pseudonana (phytoplankton)	CCMP 1014	pCO2=750 ppm	1	30	450	90	464000	19.7	0.445	276	267	-0.000039	0.592	1.000	3.070	0.056	0.108	1.288	0.072	0.123	0.208	0.054	0.017	0.028		0.309	0.003	0.048	5.23	5.79	18	35	2098	7.91	779	2265	1973	98	27	21	53	27	7.79	26	761	758	1971	101	2245	1.56	2.42
18	Thalassiosira pseudonana (phytoplankton)	CCMP 1014	pCO2=750 ppm	2	30	450	90	287000	19.7	0.634	288	279	-0.000032	0.599	0.962	4.100	0.044	0.100	1.343	0.069	0.104	0.185	0.040	0.017	0.029		0.312	0.000	0.046	6.74	5.61	18	35	1994	7.91	732	2159	1875	94	25	21	53	27	7.79	25	723	721	1873	96	2137	1.48	2.30
18	Thalassiosira pseudonana (phytoplankton)	CCMP 1014	pCO2=750 ppm	3	30	450	90	332000	19.7	0.856	295	281	-0.000045	0.622	0.605	2.890	0.048	0.105	1.334	0.088	0.129	0.200	0.046	0.017	0.027		0.318	0.001	0.046	5.16	4.22	18	35	1989	7.91	731	2154	1870	94	25	21	53	27	7.79	25	721	719	1868	96	2132	1.48	2.29
19	Thalassiosira pseudonana (phytoplankton)	CCMP 1335	pCO2=390 ppm	1	30	450	90	68500	64.9	0.428	264		-0.000011	0.430	0.703	41.900	0.050	0.085	0.246	0.020	0.111	0.010	0.028	0.024	0.023		0.312	0.004	0.052	20.60	5.36	18	35	1940	8.22	333	2252	1748	181	11	21	53	27	8.10	11	330	329	1746	183	2227	2.83	4.37
19	Thalassiosira pseudonana (phytoplankton)	CCMP 1335	pCO2=390 ppm	2	30	450	90	105000	66.7	0.588	272		-0.000026	0.386	0.676	22.200	0.058	0.085	0.253	0.055	0.086	0.025	0.049	0.032	0.025		0.310	0.000	0.052	17.10	5.46	18	35	1954	8.20	357	2252	1769	173	12	21	53	27	8.08	12	349	348	1765	177	2231	2.73	4.22
19	Thalassiosira pseudonana (phytoplankton)	CCMP 1335	pCO2=390 ppm	3	30	450	90	91000	56.9	0.378	277		-0.000006	0.421	0.496	30.000	0.038	0.058	0.155	0.025	0.056	0.012	0.030	0.022	0.024		0.323	0.016	0.050	19.00	4.99	18	35	1970	8.17	388	2252	1794	163	13	21	53	27	8.05	13	380	378	1790	167	2231	2.58	3.99
19	Thalassiosira pseudonana (phytoplankton)	CCMP 1335	pCO2=390 ppm	4	30	450	90	372000	17.7	0.664	342		-0.000034	0.527	0.488	2.190	0.139	0.222	1.172	0.114	0.417	0.076	0.047	0.019	0.027		0.311	0.001	0.048	4.86	6.42	18	35	1997	8.20	361	2301	1806	178	12	21	53	27	8.08	12	357	356	1804	181	2277	2.79	4.31
20	Thalassiosira pseudonana (phytoplankton)	CCMP 1335	pCO2=750 ppm	1	30	450	90	470000	61.6	0.838	269		-0.000025	0.645	0.538	7.680	0.074	0.173	1.051	0.118	0.317	0.070	0.122	0.029	0.024		0.320	0.002	0.046	10.10	8.31	18	35	2156	7.95	724	2343	2021	111	25	21	53	27	7.83	24	710	708	2018	114	2322	1.75	2.71
20	Thalassiosira pseudonana (phytoplankton)	CCMP 1335	pCO2=750 ppm	2	30	450	90	110000	15.9	0.693	311	305	-0.000031	0.441	0.290	5.910	0.132	0.270	2.480	0.060	0.074	0.129	0.042	0.013	0.037		0.332	0.043	0.031	23.10	4.57	18	35	2005	7.92	713	2176	1883	98	25	21	53	27	7.80	24	710	707	1882	99	2153	1.53	2.36
20	Thalassiosira pseudonana (phytoplankton)	CCMP 1335	pCO2=750 ppm	3	30	450	90	86300	15.5	0.618	316	315	-0.000035	0.446	0.203	7.700	0.136	0.304	2.279	0.032	0.081	0.107	0.042	0.011	0.039		0.334	0.003	0.038	21.20	5.35	18	35	2044	7.94	701	2223	1917	103	24	21	53	27	7.82	24	690	687	1915	105	2201	1.63	2.52
20	Thalassiosira pseudonana (phytoplankton)	CCMP 1335	pCO2=750 ppm	4	30	450	90	120000	15.5	0.705	299	295	-0.000043	0.542	0.487	5.770		0.431	2.210	0.040	0.105	0.108	0.045	0.010	0.039		0.325	0.002	0.043	15.10	5.97	18	35	1972	7.96	650	2154	1847	103	22	21	53	27	7.84	22	634	632	1844	106	2134	1.64	2.54
21	Emiliania huxleyi (phytoplankton)		pCO2=390 ppm	1	30	450	90	429000	15.9	0.430	300		-0.000103	0.256	0.303	8.850	0.009	0.046	0.031	0.013	0.221		0.042		0.128	0.104	0.210	0.002	0.043			18	35	2007	8.09	477	2252	1850	141	16	21	53	27	7.97	16	471	469	1847	144	2229	2.22	3.43
21	Emiliania huxleyi (phytoplankton)		pCO2=390 ppm	2	30	450	90	101000	27.8	0.561	319		-0.000135	0.177	0.325								0.029		0.141	0.091	0.186	0.012	0.054			18	35	1914	8.15	390	2184	1747	154	13	21	53	27	8.03	13	387	386	1745	156	2160	2.40	3.72
21	Emiliania huxleyi (phytoplankton)		pCO2=390 ppm	3	30	450	90	487000	19.7	0.683	367		-0.000103	0.277	0.255								0.031		0.111	0.091	0.203	0.003	0.043			18	35	1973	8.11	446	2226	1813	145	15	21	53	27	7.99	15	441	439	1810	147	2203	2.28	3.52
21	Emiliania huxleyi (phytoplankton)		pCO2=390 ppm	4	30	450	90	276000	19.2	0.605	390	192	-0.000105	0.337	0.260	10.800	0.007	0.052	0.011	0.007	0.247		0.040		0.127	0.106	0.199	0.003	0.048			18	35	2000	8.13	434	2263	1833	152	15	21	53	27	8.01	15	425	424	1829	156	2242	2.41	3.72
22	Emiliania huxleyi (phytoplankton)		pCO2=750 ppm	1	30	450	90	487000	20.8	0.140	430		-0.000109	0.324	0.347	7.110	0.010	0.049	0.035	0.005	0.229		0.033		0.138	0.094	0.210	0.016	0.074			18	35	2082	7.85	888	2226	1966	86	30	21	53	27	7.73	30	871	868	1964	88	2206	1.36	2.10
22	Emiliania huxleyi (phytoplankton)		pCO2=750 ppm	2	30	450	90	367000	16.8	0.735	335	356	-0.000117	0.349	0.441	8.090	0.005	0.037	0.053	0.005	0.215		0.037		0.128	0.100	0.192	0.003	0.050			18	35	2084	7.87	845	2236	1965	90	29	21	53	27	7.75	28	831	828	1964	92	2215	1.42	2.20
22	Emiliania huxleyi (phytoplankton)		pCO2=750 ppm	3	30	450	90	293000	18.7	0.646	354	347	-0.000098	0.360	0.563	9.570	0.004	0.039	0.070	0.005	0.216		0.031		0.136	0.096	0.112	0.003	0.063			18	35	1894	7.89	739	2047	1784	85	25	21	53	27	7.77	25	720	718	1782	87	2026	1.35	2.09
22	Emiliania huxleyi (phytoplankton)		pCO2=750 ppm	4	30	450	90	223000	11.1	0.858	344		-0.000104	0.324	0.377	16.500	0.004	0.059	0.055	0.007	0.202		0.047		0.132	0.108	0.209	0.005	0.048			18	35	1932	7.88	767	2083	1821	85	26	21	53	27	7.76	26	752	750	1819	87	2062	1.35	2.08