Not logged in
PANGAEA.
Data Publisher for Earth & Environmental Science

Lohbeck, Kai T; Riebesell, Ulf; Collins, Sinéad; Reusch, Thorsten BH (2012): Seawater carbonate chemistry and growth rate of Emiliania huxleyi in lab experiment. doi:10.1594/PANGAEA.823153,
Supplement to: Lohbeck, KT et al. (2013): Functional genetic divergence in high CO2 adapted Emiliania Huxleyi populations. Evolution, 67(7), 1892-1900, doi:10.1111/j.1558-5646.2012.01812.x

Always quote above citation when using data! You can download the citation in several formats below.

RIS CitationBibTeX Citation

Abstract:
Predicting the impacts of environmental change on marine organisms, food webs, and biogeochemical cycles presently relies almost exclusively on short-term physiological studies, while the possibility of adaptive evolution is often ignored. Here, we assess adaptive evolution in the coccolithophore Emiliania huxleyi, a well-established model species in biological oceanography, in response to ocean acidification. We previously demonstrated that this globally important marine phytoplankton species adapts within 500 generations to elevated CO2. After 750 and 1000 generations, no further fitness increase occurred, and we observed phenotypic convergence between replicate populations. We then exposed adapted populations to two novel environments to investigate whether or not the underlying basis for high CO2-adaptation involves functional genetic divergence, assuming that different novel mutations become apparent via divergent pleiotropic effects. The novel environment "high light" did not reveal such genetic divergence whereas growth in a low-salinity environment revealed strong pleiotropic effects in high CO2 adapted populations, indicating divergent genetic bases for adaptation to high CO2. This suggests that pleiotropy plays an important role in adaptation of natural E. huxleyi populations to ocean acidification. Our study highlights the potential mutual benefits for oceanography and evolutionary biology of using ecologically important marine phytoplankton for microbial evolution experiments.
Further details:
Lavigne, Héloise; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-11-28.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1SpeciesSpeciesLohbeck, Kai T
2TreatmentTreatLohbeck, Kai T
3GenerationGeneration#Lohbeck, Kai T
4Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmLohbeck, Kai Tselection environment
5Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmLohbeck, Kai Tassay environment
6PopulationPopul#Lohbeck, Kai T
7ReplicatesRepl#Lohbeck, Kai T
8Growth rateµ1/dayLohbeck, Kai T
9SalinitySalLohbeck, Kai Tselection environment
10Temperature, waterTemp°CLohbeck, Kai Tselection environment
11Alkalinity, totalATµmol/kgLohbeck, Kai TPotentiometric titrationselection environment
12Carbon, inorganic, dissolvedDICµmol/kgLohbeck, Kai Tselection environment
13pHpHLohbeck, Kai TCalculated using CO2SYSselection environment, total scale
14Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmLohbeck, Kai TCalculated using CO2SYSselection environment
15Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmLohbeck, Kai TCalculated using CO2SYSselection environment
16Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
17pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)selection environment, total scale
18Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)selection environment
19Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)selection environment
20Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)selection environment
21Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)selection environment
22Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)selection environment
23Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)selection environment
24Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)selection environment
25Alkalinity, totalATµmol/kgLohbeck, Kai TPotentiometric titrationassay environment
26Carbon, inorganic, dissolvedDICµmol/kgLohbeck, Kai Tassay environment
27pHpHLohbeck, Kai TCalculated using CO2SYSassay environment, total scale
28Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmLohbeck, Kai TCalculated using CO2SYSassay environment
29Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmLohbeck, Kai TCalculated using CO2SYSassay environment
30Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)assay environment
31pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)assay environment
32Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)assay environment
33Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)assay environment
34Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)assay environment
35Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)assay environment
36Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)assay environment
37Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)assay environment
38Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)assay environment
Size:
4800 data points

Download Data

Download dataset as tab-delimited text (use the following character encoding: )

View dataset as HTML (shows only first 2000 rows)