Data Description

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Citation:
Lohbeck, KT et al. (2012): Seawater carbonate chemistry and growth rate of Emiliania huxleyi in lab experiment. doi:10.1594/PANGAEA.823153,
Supplement to: Lohbeck, Kai T; Riebesell, Ulf; Collins, Sinéad; Reusch, Thorsten BH (2013): Functional genetic divergence in high CO2 adapted Emiliania Huxleyi populations. Evolution, 67(7), 1892-1900, doi:10.1111/j.1558-5646.2012.01812.x
Abstract:
Predicting the impacts of environmental change on marine organisms, food webs, and biogeochemical cycles presently relies almost exclusively on short-term physiological studies, while the possibility of adaptive evolution is often ignored. Here, we assess adaptive evolution in the coccolithophore Emiliania huxleyi, a well-established model species in biological oceanography, in response to ocean acidification. We previously demonstrated that this globally important marine phytoplankton species adapts within 500 generations to elevated CO2. After 750 and 1000 generations, no further fitness increase occurred, and we observed phenotypic convergence between replicate populations. We then exposed adapted populations to two novel environments to investigate whether or not the underlying basis for high CO2-adaptation involves functional genetic divergence, assuming that different novel mutations become apparent via divergent pleiotropic effects. The novel environment "high light" did not reveal such genetic divergence whereas growth in a low-salinity environment revealed strong pleiotropic effects in high CO2 adapted populations, indicating divergent genetic bases for adaptation to high CO2. This suggests that pleiotropy plays an important role in adaptation of natural E. huxleyi populations to ocean acidification. Our study highlights the potential mutual benefits for oceanography and evolutionary biology of using ecologically important marine phytoplankton for microbial evolution experiments.
Further details:
Lavigne, Héloise; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb *
Project(s):
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-11-28.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethodComment
1Species *SpeciesLohbeck, Kai T *
2Treatment *TreatmLohbeck, Kai T *
3Generation *Generation#Lohbeck, Kai T *
4Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmLohbeck, Kai T *selection environment
5Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmLohbeck, Kai T *assay environment
6Population *Popul#Lohbeck, Kai T *
7Replicates *Repl#Lohbeck, Kai T *
8Growth rate *µ1/dayLohbeck, Kai T *
9Salinity *SalLohbeck, Kai T *selection environment
10Temperature, water *Temp°CLohbeck, Kai T *selection environment
11Alkalinity, total *ATµmol/kgLohbeck, Kai T *Potentiometric titration *selection environment
12Carbon, inorganic, dissolved *DICµmol/kgLohbeck, Kai T *selection environment
13pH *pHLohbeck, Kai T *Calculated using CO2SYS *selection environment, total scale
14Fugacity of carbon dioxide (water) at sea surface temperature (wet air) *fCO2water_SST_wetµatmLohbeck, Kai T *Calculated using CO2SYS *selection environment
15Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmLohbeck, Kai T *Calculated using CO2SYS *selection environment
16Carbonate system computation flag *CSC flagYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *
17pH *pHYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *selection environment, total scale
18Carbon dioxide *CO2µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *selection environment
19Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *selection environment
20Fugacity of carbon dioxide (water) at sea surface temperature (wet air) *fCO2water_SST_wetµatmYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *selection environment
21Bicarbonate ion *[HCO3]-µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *selection environment
22Carbonate ion *[CO3]2-µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *selection environment
23Aragonite saturation state *Omega ArgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *selection environment
24Calcite saturation state *Omega CalYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *selection environment
25Alkalinity, total *ATµmol/kgLohbeck, Kai T *Potentiometric titration *assay environment
26Carbon, inorganic, dissolved *DICµmol/kgLohbeck, Kai T *assay environment
27pH *pHLohbeck, Kai T *Calculated using CO2SYS *assay environment, total scale
28Fugacity of carbon dioxide (water) at sea surface temperature (wet air) *fCO2water_SST_wetµatmLohbeck, Kai T *Calculated using CO2SYS *assay environment
29Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmLohbeck, Kai T *Calculated using CO2SYS *assay environment
30Carbonate system computation flag *CSC flagYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *assay environment
31pH *pHYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *assay environment
32Carbon dioxide *CO2µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *assay environment
33Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) *pCO2water_SST_wetµatmYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *assay environment
34Fugacity of carbon dioxide (water) at sea surface temperature (wet air) *fCO2water_SST_wetµatmYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *assay environment
35Bicarbonate ion *[HCO3]-µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *assay environment
36Carbonate ion *[CO3]2-µmol/kgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *assay environment
37Aragonite saturation state *Omega ArgYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *assay environment
38Calcite saturation state *Omega CalYang, Yan *Calculated using seacarb after Nisumaa et al. (2010) *assay environment
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4800 data points

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