Citation:

j.1558-5646.2012.01812.x

Name: Seawater carbonate chemistry and growth rate of Emiliania huxleyi in lab experiment
Published: 2013-11-28
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Bottles or small containers/Aquaria (<20 L); Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Light; North Atlantic; Pelagos; Phytoplankton; Salinity; Single species

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Abstract:

Predicting the impacts of environmental change on marine organisms, food webs, and biogeochemical cycles presently relies almost exclusively on short-term physiological studies, while the possibility of adaptive evolution is often ignored. Here, we assess adaptive evolution in the coccolithophore Emiliania huxleyi, a well-established model species in biological oceanography, in response to ocean acidification. We previously demonstrated that this globally important marine phytoplankton species adapts within 500 generations to elevated CO2. After 750 and 1000 generations, no further fitness increase occurred, and we observed phenotypic convergence between replicate populations. We then exposed adapted populations to two novel environments to investigate whether or not the underlying basis for high CO2-adaptation involves functional genetic divergence, assuming that different novel mutations become apparent via divergent pleiotropic effects. The novel environment "high light" did not reveal such genetic divergence whereas growth in a low-salinity environment revealed strong pleiotropic effects in high CO2 adapted populations, indicating divergent genetic bases for adaptation to high CO2. This suggests that pleiotropy plays an important role in adaptation of natural E. huxleyi populations to ocean acidification. Our study highlights the potential mutual benefits for oceanography and evolutionary biology of using ecologically important marine phytoplankton for microbial evolution experiments.

Keyword(s):

Bottles or small containers/Aquaria (<20 L); Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Light; North Atlantic; Pelagos; Phytoplankton; Salinity; Single species

Further details:

Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb

Project(s):

Biological Impacts of Ocean Acidification (BIOACID)

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-11-28.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Lohbeck, Kai T
2	Treatment	Treat		Lohbeck, Kai T
3	Generation	Generation	#	Lohbeck, Kai T
4	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Lohbeck, Kai T		selection environment
5	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Lohbeck, Kai T		assay environment
6	Population	Popul	#	Lohbeck, Kai T
7	Replicates	Repl	#	Lohbeck, Kai T
8	Growth rate	µ	1/day	Lohbeck, Kai T
9	Salinity	Sal		Lohbeck, Kai T		selection environment
10	Temperature, water	Temp	°C	Lohbeck, Kai T		selection environment
11	Alkalinity, total	AT	µmol/kg	Lohbeck, Kai T	Potentiometric titration	selection environment
12	Carbon, inorganic, dissolved	DIC	µmol/kg	Lohbeck, Kai T		selection environment
13	pH	pH		Lohbeck, Kai T	Calculated using CO2SYS	selection environment, total scale
14	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Lohbeck, Kai T	Calculated using CO2SYS	selection environment
15	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Lohbeck, Kai T	Calculated using CO2SYS	selection environment
16	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
17	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	selection environment, total scale
18	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	selection environment
19	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	selection environment
20	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	selection environment
21	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	selection environment
22	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	selection environment
23	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	selection environment
24	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	selection environment
25	Alkalinity, total	AT	µmol/kg	Lohbeck, Kai T	Potentiometric titration	assay environment
26	Carbon, inorganic, dissolved	DIC	µmol/kg	Lohbeck, Kai T		assay environment
27	pH	pH		Lohbeck, Kai T	Calculated using CO2SYS	assay environment, total scale
28	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Lohbeck, Kai T	Calculated using CO2SYS	assay environment
29	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Lohbeck, Kai T	Calculated using CO2SYS	assay environment
30	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	assay environment
31	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	assay environment
32	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	assay environment
33	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	assay environment
34	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	assay environment
35	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	assay environment
36	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	assay environment
37	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	assay environment
38	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	assay environment

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

4800 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Species	2 Treat	3 Generation [#]	4 pCO2water_SST_wet [µatm] (selection environment)	5 pCO2water_SST_wet [µatm] (assay environment)	6 Popul [#]	7 Repl [#]	8 µ [1/day]	9 Sal (selection environment)	10 Temp [°C] (selection environment)	11 AT [µmol/kg] (selection environment, Potent...)	12 DIC [µmol/kg] (selection environment)	13 pH (selection environment, total ...)	14 fCO2water_SST_wet [µatm] (selection environment, Calcul...)	15 pCO2water_SST_wet [µatm] (selection environment, Calcul...)	16 CSC flag (Calculated using seacarb afte...)	17 pH (selection environment, total ...)	18 CO2 [µmol/kg] (selection environment, Calcul...)	19 pCO2water_SST_wet [µatm] (selection environment, Calcul...)	20 fCO2water_SST_wet [µatm] (selection environment, Calcul...)	21 [HCO3]- [µmol/kg] (selection environment, Calcul...)	22 [CO3]2- [µmol/kg] (selection environment, Calcul...)	23 Omega Arg (selection environment, Calcul...)	24 Omega Cal (selection environment, Calcul...)	25 AT [µmol/kg] (assay environment, Potentiome...)	26 DIC [µmol/kg] (assay environment)	27 pH (assay environment, total scal...)	28 fCO2water_SST_wet [µatm] (assay environment, Calculated...)	29 pCO2water_SST_wet [µatm] (assay environment, Calculated...)	30 CSC flag (assay environment, Calculated...)	31 pH (assay environment, Calculated...)	32 CO2 [µmol/kg] (assay environment, Calculated...)	33 pCO2water_SST_wet [µatm] (assay environment, Calculated...)	34 fCO2water_SST_wet [µatm] (assay environment, Calculated...)	35 [HCO3]- [µmol/kg] (assay environment, Calculated...)	36 [CO3]2- [µmol/kg] (assay environment, Calculated...)	37 Omega Arg (assay environment, Calculated...)	38 Omega Cal (assay environment, Calculated...)
Emiliania huxleyi (phytoplankton)		500	400	400	1		1.102	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		500	400	400	2		1.097	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		500	400	400	3		1.081	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		500	400	400	4		1.099	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		500	400	400	5		1.083	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		500	2200	400	1		1.108	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		500	2200	400	2		1.122	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		500	2200	400	3		1.106	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		500	2200	400	4		1.119	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		500	2200	400	5		1.116	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		500	2200	2200	1		0.910	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		500	2200	2200	2		0.955	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		500	2200	2200	3		0.943	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		500	2200	2200	4		0.936	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		500	2200	2200	5		0.931	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		500	400	2200	1		0.908	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		500	400	2200	2		0.897	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		500	400	2200	3		0.923	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		500	400	2200	4		0.890	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		500	400	2200	5		0.852	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		750	400	400	1		1.168	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		750	400	400	2		1.134	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		750	400	400	3		1.130	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		750	400	400	4		1.147	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		750	400	400	5		1.136	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		750	2200	400	1		1.096	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		750	2200	400	2		1.109	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		750	2200	400	3		1.091	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		750	2200	400	4		1.115	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		750	2200	400	5		1.098	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		750	2200	2200	1		0.949	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		750	2200	2200	2		0.993	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		750	2200	2200	3		0.988	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		750	2200	2200	4		0.983	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		750	2200	2200	5		0.966	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		750	400	2200	1		0.952	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		750	400	2200	2		0.932	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		750	400	2200	3		0.957	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		750	400	2200	4		0.953	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		750	400	2200	5		0.934	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		1000	400	400	1		1.172	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		1000	400	400	2		1.179	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		1000	400	400	3		1.170	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		1000	400	400	4		1.202	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		1000	400	400	5		1.228	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		1000	2200	400	1		1.181	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		1000	2200	400	2		1.157	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		1000	2200	400	3		1.152	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		1000	2200	400	4		1.140	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		1000	2200	400	5		1.118	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)		1000	2200	2200	1		1.009	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		1000	2200	2200	2		0.975	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		1000	2200	2200	3		1.025	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		1000	2200	2200	4		0.968	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		1000	2200	2200	5		0.972	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		1000	400	2200	1		0.978	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		1000	400	2200	2		0.947	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		1000	400	2200	3		0.983	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		1000	400	2200	4		0.958	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)		1000	400	2200	5		0.950	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control light		400		1	1	1.165	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control light		400		1	2	1.162	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control light		400		1	3	1.173	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control light		400		2	1	1.155	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control light		400		2	2	1.159	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control light		400		2	3	1.161	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control light		400		3	1	1.155	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control light		400		3	2	1.164	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control light		400		3	3	1.151	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control light		400		4	1	1.152	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control light		400		4	2	1.156	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control light		400		4	3	1.169	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control light		400		5	1	1.159	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control light		400		5	2	1.177	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control light		400		5	3	1.163	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	High light		400		1	1	1.225	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	High light		400		1	2	1.200	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	High light		400		1	3	1.179	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	High light		400		2	1	1.194	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	High light		400		2	2	1.211	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	High light		400		2	3	1.204	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	High light		400		3	1	1.205	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	High light		400		3	2	1.183	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	High light		400		3	3	1.206	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	High light		400		4	1	1.211	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	High light		400		4	2	1.199	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	High light		400		4	3	1.210	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	High light		400		5	1	1.188	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	High light		400		5	2	1.201	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	High light		400		5	3	1.190	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control light		2200		1	1	0.966	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control light		2200		1	2	0.972	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control light		2200		1	3	0.968	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control light		2200		2	1	0.972	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control light		2200		2	2	0.968	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control light		2200		2	3	0.951	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control light		2200		3	1	0.977	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control light		2200		3	2	0.974	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control light		2200		3	3	0.976	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control light		2200		4	1	0.953	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control light		2200		4	2	0.946	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control light		2200		4	3	0.938	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control light		2200		5	1	0.982	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control light		2200		5	2	0.972	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control light		2200		5	3	0.959	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	High light		2200		1	1	1.041	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	High light		2200		1	2	1.038	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	High light		2200		1	3	1.026	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	High light		2200		2	1	1.028	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	High light		2200		2	2	1.039	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	High light		2200		2	3	1.030	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	High light		2200		3	1	1.050	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	High light		2200		3	2	1.060	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	High light		2200		3	3	1.037	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	High light		2200		4	1	1.023	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	High light		2200		4	2	1.043	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	High light		2200		4	3	1.019	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	High light		2200		5	1	1.041	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	High light		2200		5	2	1.053	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	High light		2200		5	3	1.026	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control salinity		400		1	1	1.137	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control salinity		400		1	2	1.147	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control salinity		400		1	3	1.156	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control salinity		400		2	1	1.135	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control salinity		400		2	2	1.142	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control salinity		400		2	3	1.140	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control salinity		400		3	1	1.137	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control salinity		400		3	2	1.126	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control salinity		400		3	3	1.124	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control salinity		400		4	1	1.123	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control salinity		400		4	2	1.135	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control salinity		400		4	3	1.120	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control salinity		400		5	1	1.124	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control salinity		400		5	2	1.111	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Control salinity		400		5	3	1.110	35	15	2426.8	2141.1	8.13	341.7	342.9	15	8.15	11.9	319.8	318.6	1927.7	201.5	3.08	4.80
Emiliania huxleyi (phytoplankton)	Low salinity		400		1	1	0.846	15	15	2356.9	2194.8	8.19	358.6	359.9	15	8.20	14.5	347.8	346.6	2041.9	138.4	2.20	3.74
Emiliania huxleyi (phytoplankton)	Low salinity		400		1	2	0.863	15	15	2356.9	2194.8	8.19	358.6	359.9	15	8.20	14.5	347.8	346.6	2041.9	138.4	2.20	3.74
Emiliania huxleyi (phytoplankton)	Low salinity		400		1	3	0.852	15	15	2356.9	2194.8	8.19	358.6	359.9	15	8.20	14.5	347.8	346.6	2041.9	138.4	2.20	3.74
Emiliania huxleyi (phytoplankton)	Low salinity		400		2	1	0.855	15	15	2356.9	2194.8	8.19	358.6	359.9	15	8.20	14.5	347.8	346.6	2041.9	138.4	2.20	3.74
Emiliania huxleyi (phytoplankton)	Low salinity		400		2	2	0.841	15	15	2356.9	2194.8	8.19	358.6	359.9	15	8.20	14.5	347.8	346.6	2041.9	138.4	2.20	3.74
Emiliania huxleyi (phytoplankton)	Low salinity		400		2	3	0.850	15	15	2356.9	2194.8	8.19	358.6	359.9	15	8.20	14.5	347.8	346.6	2041.9	138.4	2.20	3.74
Emiliania huxleyi (phytoplankton)	Low salinity		400		3	1	0.859	15	15	2356.9	2194.8	8.19	358.6	359.9	15	8.20	14.5	347.8	346.6	2041.9	138.4	2.20	3.74
Emiliania huxleyi (phytoplankton)	Low salinity		400		3	2	0.857	15	15	2356.9	2194.8	8.19	358.6	359.9	15	8.20	14.5	347.8	346.6	2041.9	138.4	2.20	3.74
Emiliania huxleyi (phytoplankton)	Low salinity		400		3	3	0.858	15	15	2356.9	2194.8	8.19	358.6	359.9	15	8.20	14.5	347.8	346.6	2041.9	138.4	2.20	3.74
Emiliania huxleyi (phytoplankton)	Low salinity		400		4	1	0.869	15	15	2356.9	2194.8	8.19	358.6	359.9	15	8.20	14.5	347.8	346.6	2041.9	138.4	2.20	3.74
Emiliania huxleyi (phytoplankton)	Low salinity		400		4	2	0.868	15	15	2356.9	2194.8	8.19	358.6	359.9	15	8.20	14.5	347.8	346.6	2041.9	138.4	2.20	3.74
Emiliania huxleyi (phytoplankton)	Low salinity		400		4	3	0.874	15	15	2356.9	2194.8	8.19	358.6	359.9	15	8.20	14.5	347.8	346.6	2041.9	138.4	2.20	3.74
Emiliania huxleyi (phytoplankton)	Low salinity		400		5	1	0.846	15	15	2356.9	2194.8	8.19	358.6	359.9	15	8.20	14.5	347.8	346.6	2041.9	138.4	2.20	3.74
Emiliania huxleyi (phytoplankton)	Low salinity		400		5	2	0.842	15	15	2356.9	2194.8	8.19	358.6	359.9	15	8.20	14.5	347.8	346.6	2041.9	138.4	2.20	3.74
Emiliania huxleyi (phytoplankton)	Low salinity		400		5	3	0.844	15	15	2356.9	2194.8	8.19	358.6	359.9	15	8.20	14.5	347.8	346.6	2041.9	138.4	2.20	3.74
Emiliania huxleyi (phytoplankton)	Control salinity		2200		1	1	0.978	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control salinity		2200		1	2	0.990	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control salinity		2200		1	3	0.969	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control salinity		2200		2	1	0.985	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control salinity		2200		2	2	0.986	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control salinity		2200		2	3	0.968	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control salinity		2200		3	1	0.987	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control salinity		2200		3	2	0.980	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control salinity		2200		3	3	0.986	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control salinity		2200		4	1	0.977	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control salinity		2200		4	2	0.966	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control salinity		2200		4	3	0.972	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control salinity		2200		5	1	0.976	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control salinity		2200		5	2	0.984	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Control salinity		2200		5	3	0.976	35	15	2438.1	2435.7	7.44	1985.2	1992.4	15	7.45	71.8	1923.3	1916.4	2315.6	48.3	0.74	1.15
Emiliania huxleyi (phytoplankton)	Low salinity		2200		1	1	0.000	15	15	2364.9	2413.4	7.47	2130.1	2137.8	15	7.47	88.3	2116.0	2108.4	2296.3	28.8	0.46	0.78
Emiliania huxleyi (phytoplankton)	Low salinity		2200		1	2	0.000	15	15	2364.9	2413.4	7.47	2130.1	2137.8	15	7.47	88.3	2116.0	2108.4	2296.3	28.8	0.46	0.78
Emiliania huxleyi (phytoplankton)	Low salinity		2200		1	3	0.000	15	15	2364.9	2413.4	7.47	2130.1	2137.8	15	7.47	88.3	2116.0	2108.4	2296.3	28.8	0.46	0.78
Emiliania huxleyi (phytoplankton)	Low salinity		2200		2	1	0.318	15	15	2364.9	2413.4	7.47	2130.1	2137.8	15	7.47	88.3	2116.0	2108.4	2296.3	28.8	0.46	0.78
Emiliania huxleyi (phytoplankton)	Low salinity		2200		2	2	0.280	15	15	2364.9	2413.4	7.47	2130.1	2137.8	15	7.47	88.3	2116.0	2108.4	2296.3	28.8	0.46	0.78
Emiliania huxleyi (phytoplankton)	Low salinity		2200		2	3	0.331	15	15	2364.9	2413.4	7.47	2130.1	2137.8	15	7.47	88.3	2116.0	2108.4	2296.3	28.8	0.46	0.78
Emiliania huxleyi (phytoplankton)	Low salinity		2200		3	1	0.452	15	15	2364.9	2413.4	7.47	2130.1	2137.8	15	7.47	88.3	2116.0	2108.4	2296.3	28.8	0.46	0.78
Emiliania huxleyi (phytoplankton)	Low salinity		2200		3	2	0.429	15	15	2364.9	2413.4	7.47	2130.1	2137.8	15	7.47	88.3	2116.0	2108.4	2296.3	28.8	0.46	0.78
Emiliania huxleyi (phytoplankton)	Low salinity		2200		3	3	0.418	15	15	2364.9	2413.4	7.47	2130.1	2137.8	15	7.47	88.3	2116.0	2108.4	2296.3	28.8	0.46	0.78
Emiliania huxleyi (phytoplankton)	Low salinity		2200		4	1	0.308	15	15	2364.9	2413.4	7.47	2130.1	2137.8	15	7.47	88.3	2116.0	2108.4	2296.3	28.8	0.46	0.78
Emiliania huxleyi (phytoplankton)	Low salinity		2200		4	2	0.338	15	15	2364.9	2413.4	7.47	2130.1	2137.8	15	7.47	88.3	2116.0	2108.4	2296.3	28.8	0.46	0.78
Emiliania huxleyi (phytoplankton)	Low salinity		2200		4	3	0.357	15	15	2364.9	2413.4	7.47	2130.1	2137.8	15	7.47	88.3	2116.0	2108.4	2296.3	28.8	0.46	0.78
Emiliania huxleyi (phytoplankton)	Low salinity		2200		5	1	0.259	15	15	2364.9	2413.4	7.47	2130.1	2137.8	15	7.47	88.3	2116.0	2108.4	2296.3	28.8	0.46	0.78
Emiliania huxleyi (phytoplankton)	Low salinity		2200		5	2	0.265	15	15	2364.9	2413.4	7.47	2130.1	2137.8	15	7.47	88.3	2116.0	2108.4	2296.3	28.8	0.46	0.78
Emiliania huxleyi (phytoplankton)	Low salinity		2200		5	3	0.271	15	15	2364.9	2413.4	7.47	2130.1	2137.8	15	7.47	88.3	2116.0	2108.4	2296.3	28.8	0.46	0.78