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Fehsenfeld, Sandra; Weihrauch, Dirk (2013): Differential acid-base regulation in various gills of the green crab Carcinus maenas: Effects of elevated environmental pCO2 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.823109, Supplement to: Fehsenfeld, S; Weihrauch, D (2013): Differential acid–base regulation in various gills of the green crab Carcinus maenas: Effects of elevated environmental pCO2. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 164(1), 54-65, https://doi.org/10.1016/j.cbpa.2012.09.016

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Abstract:
Euryhaline decapod crustaceans possess an efficient regulation apparatus located in the gill epithelia, providing a high adaptation potential to varying environmental abiotic conditions. Even though many studies focussed on the osmoregulatory capacity of the gills, acid-base regulatory mechanisms have obtained much less attention. In the present study, underlying principles and effects of elevated pCO2 on acid-base regulatory patterns were investigated in the green crab Carcinus maenas acclimated to diluted seawater. In gill perfusion experiments, all investigated gills 4-9 were observed to up-regulate the pH of the hemolymph by 0.1-0.2 units. Anterior gills, especially gill 4, were identified to be most efficient in the equivalent proton excretion rate. Ammonia excretion rates mirrored this pattern among gills, indicating a linkage between both processes. In specimen exposed to elevated pCO2 levels for at least 7 days, mimicking a future ocean scenario as predicted until the year 2300, hemolymph K+ and ammonia concentrations were significantly elevated, and an increased ammonia excretion rate was observed. A detailed quantitative gene expression analysis revealed that upon elevated pCO2 exposure, mRNA levels of transcripts hypothesized to be involved in ammonia and acid-base regulation (Rhesus-like protein, membrane-bound carbonic anhydrase, Na+/K+-ATPase) were affected predominantly in the non-osmoregulating anterior gills.
Keyword(s):
Acid-base regulation; Animalia; Arthropoda; Benthic animals; Benthos; Carcinus maenas; Containers and aquaria (20-1000 L or < 1 m**2); Gene expression (incl. proteomics); Laboratory experiment; Laboratory strains; Not applicable; Other metabolic rates; Single species
Further details:
Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb
Project(s):
Ocean Acidification International Coordination Centre (OA-ICC)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-11-20.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1SpeciesSpeciesFehsenfeld, Sandra
2IdentificationIDFehsenfeld, Sandra
3Sample code/labelSample labelFehsenfeld, Sandra
4TreatmentTreatFehsenfeld, Sandra
5pHpHFehsenfeld, Sandraperfusion pH
6Haemolymph, pHpH (ha)Fehsenfeld, Sandra
7Haemolymph, pH, standard deviationpH (ha) std dev±Fehsenfeld, Sandra
8Decrease of haemolymph proton concentrationDecrease ha H+nmol/lFehsenfeld, Sandra
9Decrease of haemolymph proton concentration, standard deviationDecrease ha H+ std dev±Fehsenfeld, Sandra
10Ammonia excretionNH3/[NH4]+ excnmol/g/hFehsenfeld, Sandra
11Ammonia excretion, standard deviationNH3/[NH4]+ exc std dev±Fehsenfeld, Sandra
12Decrease of haemolymph proton concentration, relativeDecrease ha H+Fehsenfeld, Sandra
13Decrease of haemolymph proton concentration, relative, standard deviationDecrease ha H+ std dev±Fehsenfeld, Sandra
14Ammonia excretion, relativeNH3/[NH4]+ excFehsenfeld, Sandra
15Ammonia excretion, relative, standard deviationNH3/[NH4]+ exc std dev±Fehsenfeld, Sandra
16Protein nameProteinFehsenfeld, Sandra
17Gene expression, fold change, relativeGene expressionFehsenfeld, Sandra
18Gene expression, fold change, relative, standard deviationGene expression std dev±Fehsenfeld, Sandra
19SalinitySalFehsenfeld, Sandra
20Temperature, waterTemp°CFehsenfeld, Sandra
21pHpHFehsenfeld, SandraNBS
22Carbon, inorganic, dissolvedDICµmol/kgFehsenfeld, SandraCoulometric titration
23Carbon, inorganic, dissolved, standard deviationDIC std dev±Fehsenfeld, SandraCoulometric titration
24Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetPaFehsenfeld, Sandra
25Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Fehsenfeld, SandraCalculated using CO2SYS
26Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
27pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
28Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
29Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
30Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
31Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
32Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
33Alkalinity, totalATµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
34Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
35Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
3407 data points

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