Citation:

pcs066

Name: Reduced calcification decreases photoprotective capability in the Coccolithophorid Emiliania huxleyi
Published: 2012
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Emiliania huxleyi; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Other; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species

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Abstract:

Intracellular calcification of coccolithophores generates CO2 and consumes additional energy for acquisition of calcium and bicarbonate ions; therefore, it may correlate with photoprotective processes by influencing the energetics. To address this hypothesis, a calcifying Emiliania huxleyi strain (CS-369) was grown semi-continuously at reduced (0.1 mM, LCa) and ambient Ca2+ concentrations (10 mM, HCa) for 150 d (>200 generations). The HCa-grown cells had higher photosynthetic and calcification rates and higher contents of Chl a and carotenoids compared with the naked (bearing no coccoliths) LCa-grown cells. When exposed to stressfull levels of photosynthetically active radiation (PAR), LCa-grown cells displayed lower photochemical yield and less efficient non-photochemical quenching (NPQ). When the LCa- or HCa-grown cells were inversely shifted to their counterpart medium, LCa to HCa transfer increased photosynthetic carbon fixation (P), calcification rate (C), the C/P ratio, NPQ and pigment contents, whereas those shifted from HCa to LCa exhibited the opposite effects. Increased NPQ, carotenoids and quantum yield were clearly linked with increased or sustained calcification in E. huxleyi. The calcification must have played a role in dissipating excessive energy or as an additional drainage of electrons absorbed by the photosynthetic antennae. This phenomenon was further supported by testing two non-calcifying strains, which showed insignificant changes in photosynthetic carbon fixation and NPQ when transferred to LCa conditions

Keyword(s):

Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Emiliania huxleyi; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Other; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species

Further details:

Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-11-29.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Xu, Kai
2	Treatment	Treat		Xu, Kai
3	Figure	Fig		Xu, Kai
4	Strain	Strain		Xu, Kai
5	Incubation duration	Inc dur	days	Xu, Kai
6	Calcium	Ca	mmol/l	Xu, Kai		initial
7	Calcium	Ca	mmol/l	Xu, Kai		after transfer
8	Replicate	Repl		Xu, Kai
9	Growth rate	µ	1/day	Xu, Kai
10	Chlorophyll a per cell	Chl a/cell	pg/#	Xu, Kai
11	Carotenoids per cell	Carotenoids/cell	pg/#	Xu, Kai
12	Net photosynthesis rate, per cell	PN/cell	pg/#/h	Xu, Kai
13	Calcification rate of carbon per cell	Calc rate C/cell	pg/#/h	Xu, Kai
14	Net calcification/net photosynthesis ratio	NC/NP		Xu, Kai
15	Non photochemical quenching	NPQ		Xu, Kai
16	Duration	Duration	h	Xu, Kai
17	Maximum photochemical quantum yield of photosystem II	Fv/Fm		Xu, Kai
18	Effective quantum yield	Y		Xu, Kai
19	Photosynthesis rate, ratio	P ratio		Xu, Kai		PAR 800/PAR 150
20	Calcification rate, ratio	Calc rate ratio		Xu, Kai		PAR 800/PAR 150
21	Calcification rate/Photosynthesis rate, ratio	Calc rate/P		Xu, Kai		PAR 800/PAR 150
22	Effective absorbance cross-section of photosystem II	sigma PSII	A²/quanta	Xu, Kai
23	Photochemical efficiency	delta F/Fm	arbitrary units	Xu, Kai		the apparent photochemical efficiency
24	Maximal electron transport rate, relative	rETR max	µmol e/m²/s	Xu, Kai
25	Light saturation point	Ik		Xu, Kai
26	Temperature, water	Temp	°C	Xu, Kai
27	Salinity	Sal		Xu, Kai
28	pH	pH		Xu, Kai		NBS, initial
29	pH, standard deviation	pH std dev	±	Xu, Kai		NBS, initial
30	Alkalinity, total	AT	µmol/kg	Xu, Kai	Potentiometric titration	initial
31	Alkalinity, total, standard deviation	AT std dev	±	Xu, Kai	Potentiometric titration	initial
32	Carbon, inorganic, dissolved	DIC	µmol/kg	Xu, Kai	Calculated using CO2SYS	initial
33	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Xu, Kai	Calculated using CO2SYS	initial
34	Carbon dioxide	CO2	µmol/kg	Xu, Kai	Calculated using CO2SYS	initial
35	Carbon dioxide, standard deviation	CO2 std dev	±	Xu, Kai	Calculated using CO2SYS	initial
36	Bicarbonate ion	[HCO3]-	µmol/kg	Xu, Kai	Calculated using CO2SYS	initial
37	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Xu, Kai	Calculated using CO2SYS	initial
38	Carbonate ion	[CO3]2-	µmol/kg	Xu, Kai	Calculated using CO2SYS	initial
39	Carbonate ion, standard deviation	[CO3]2- std dev	±	Xu, Kai	Calculated using CO2SYS	initial
40	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
41	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale, initial
42	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
43	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
44	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
45	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
46	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
47	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
48	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
49	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
50	pH	pH		Xu, Kai		NBS, after transfer
51	pH, standard deviation	pH std dev	±	Xu, Kai		NBS, after transfer
52	Alkalinity, total	AT	µmol/kg	Xu, Kai	Potentiometric titration	after transfer
53	Alkalinity, total, standard deviation	AT std dev	±	Xu, Kai	Potentiometric titration	after transfer
54	Carbon, inorganic, dissolved	DIC	µmol/kg	Xu, Kai	Calculated using CO2SYS	after transfer
55	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Xu, Kai	Calculated using CO2SYS	after transfer
56	Carbon dioxide	CO2	µmol/kg	Xu, Kai	Calculated using CO2SYS	after transfer
57	Carbon dioxide, standard deviation	CO2 std dev	±	Xu, Kai	Calculated using CO2SYS	after transfer
58	Bicarbonate ion	[HCO3]-	µmol/kg	Xu, Kai	Calculated using CO2SYS	after transfer
59	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Xu, Kai	Calculated using CO2SYS	after transfer
60	Carbonate ion	[CO3]2-	µmol/kg	Xu, Kai	Calculated using CO2SYS	after transfer
61	Carbonate ion, standard deviation	[CO3]2- std dev	±	Xu, Kai	Calculated using CO2SYS	after transfer
62	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	after transfer
63	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale, after transfer
64	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	after transfer
65	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	after transfer
66	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	after transfer
67	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	after transfer
68	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	after transfer
69	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	after transfer
70	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	after transfer
71	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	after transfer

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

6738 data points

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