Citation:

pcs066

Name: Reduced calcification decreases photoprotective capability in the Coccolithophorid Emiliania huxleyi
Published: 2012
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Emiliania huxleyi; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Other; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species

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Abstract:

Intracellular calcification of coccolithophores generates CO2 and consumes additional energy for acquisition of calcium and bicarbonate ions; therefore, it may correlate with photoprotective processes by influencing the energetics. To address this hypothesis, a calcifying Emiliania huxleyi strain (CS-369) was grown semi-continuously at reduced (0.1 mM, LCa) and ambient Ca2+ concentrations (10 mM, HCa) for 150 d (>200 generations). The HCa-grown cells had higher photosynthetic and calcification rates and higher contents of Chl a and carotenoids compared with the naked (bearing no coccoliths) LCa-grown cells. When exposed to stressfull levels of photosynthetically active radiation (PAR), LCa-grown cells displayed lower photochemical yield and less efficient non-photochemical quenching (NPQ). When the LCa- or HCa-grown cells were inversely shifted to their counterpart medium, LCa to HCa transfer increased photosynthetic carbon fixation (P), calcification rate (C), the C/P ratio, NPQ and pigment contents, whereas those shifted from HCa to LCa exhibited the opposite effects. Increased NPQ, carotenoids and quantum yield were clearly linked with increased or sustained calcification in E. huxleyi. The calcification must have played a role in dissipating excessive energy or as an additional drainage of electrons absorbed by the photosynthetic antennae. This phenomenon was further supported by testing two non-calcifying strains, which showed insignificant changes in photosynthetic carbon fixation and NPQ when transferred to LCa conditions

Keyword(s):

Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Emiliania huxleyi; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Other; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species

Further details:

Lavigne, Héloïse; Gattuso, Jean-Pierre (2011): seacarb: seawater carbonate chemistry with R. R package version 2.4. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-11-29.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Species	Species		Xu, Kai
2	Treatment	Treat		Xu, Kai
3	Figure	Fig		Xu, Kai
4	Strain	Strain		Xu, Kai
5	Incubation duration	Inc dur	days	Xu, Kai
6	Calcium	Ca	mmol/l	Xu, Kai		initial
7	Calcium	Ca	mmol/l	Xu, Kai		after transfer
8	Replicate	Repl		Xu, Kai
9	Growth rate	µ	1/day	Xu, Kai
10	Chlorophyll a per cell	Chl a/cell	pg/#	Xu, Kai
11	Carotenoids per cell	Carotenoids/cell	pg/#	Xu, Kai
12	Net photosynthesis rate, per cell	PN/cell	pg/#/h	Xu, Kai
13	Calcification rate of carbon per cell	Calc rate C/cell	pg/#/h	Xu, Kai
14	Net calcification/net photosynthesis ratio	NC/NP		Xu, Kai
15	Non photochemical quenching	NPQ		Xu, Kai
16	Duration	Duration	h	Xu, Kai
17	Maximum photochemical quantum yield of photosystem II	Fv/Fm		Xu, Kai
18	Effective quantum yield	Y		Xu, Kai
19	Photosynthesis rate, ratio	P ratio		Xu, Kai		PAR 800/PAR 150
20	Calcification rate, ratio	Calc rate ratio		Xu, Kai		PAR 800/PAR 150
21	Calcification rate/Photosynthesis rate, ratio	Calc rate/P		Xu, Kai		PAR 800/PAR 150
22	Effective absorbance cross-section of photosystem II	sigma PSII	A²/quanta	Xu, Kai
23	Photochemical efficiency	delta F/Fm	arbitrary units	Xu, Kai		the apparent photochemical efficiency
24	Maximal electron transport rate, relative	rETR max	µmol e/m²/s	Xu, Kai
25	Light saturation point	Ik		Xu, Kai
26	Temperature, water	Temp	°C	Xu, Kai
27	Salinity	Sal		Xu, Kai
28	pH	pH		Xu, Kai		NBS, initial
29	pH, standard deviation	pH std dev	±	Xu, Kai		NBS, initial
30	Alkalinity, total	AT	µmol/kg	Xu, Kai	Potentiometric titration	initial
31	Alkalinity, total, standard deviation	AT std dev	±	Xu, Kai	Potentiometric titration	initial
32	Carbon, inorganic, dissolved	DIC	µmol/kg	Xu, Kai	Calculated using CO2SYS	initial
33	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Xu, Kai	Calculated using CO2SYS	initial
34	Carbon dioxide	CO2	µmol/kg	Xu, Kai	Calculated using CO2SYS	initial
35	Carbon dioxide, standard deviation	CO2 std dev	±	Xu, Kai	Calculated using CO2SYS	initial
36	Bicarbonate ion	[HCO3]-	µmol/kg	Xu, Kai	Calculated using CO2SYS	initial
37	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Xu, Kai	Calculated using CO2SYS	initial
38	Carbonate ion	[CO3]2-	µmol/kg	Xu, Kai	Calculated using CO2SYS	initial
39	Carbonate ion, standard deviation	[CO3]2- std dev	±	Xu, Kai	Calculated using CO2SYS	initial
40	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
41	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale, initial
42	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
43	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
44	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
45	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
46	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
47	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
48	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
49	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	initial
50	pH	pH		Xu, Kai		NBS, after transfer
51	pH, standard deviation	pH std dev	±	Xu, Kai		NBS, after transfer
52	Alkalinity, total	AT	µmol/kg	Xu, Kai	Potentiometric titration	after transfer
53	Alkalinity, total, standard deviation	AT std dev	±	Xu, Kai	Potentiometric titration	after transfer
54	Carbon, inorganic, dissolved	DIC	µmol/kg	Xu, Kai	Calculated using CO2SYS	after transfer
55	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Xu, Kai	Calculated using CO2SYS	after transfer
56	Carbon dioxide	CO2	µmol/kg	Xu, Kai	Calculated using CO2SYS	after transfer
57	Carbon dioxide, standard deviation	CO2 std dev	±	Xu, Kai	Calculated using CO2SYS	after transfer
58	Bicarbonate ion	[HCO3]-	µmol/kg	Xu, Kai	Calculated using CO2SYS	after transfer
59	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Xu, Kai	Calculated using CO2SYS	after transfer
60	Carbonate ion	[CO3]2-	µmol/kg	Xu, Kai	Calculated using CO2SYS	after transfer
61	Carbonate ion, standard deviation	[CO3]2- std dev	±	Xu, Kai	Calculated using CO2SYS	after transfer
62	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	after transfer
63	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale, after transfer
64	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	after transfer
65	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	after transfer
66	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	after transfer
67	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	after transfer
68	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	after transfer
69	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	after transfer
70	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	after transfer
71	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	after transfer

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

6738 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Species	2 Treat	3 Fig	4 Strain	5 Inc dur [days]	6 Ca [mmol/l] (initial)	7 Ca [mmol/l] (after transfer)	8 Repl	9 µ [1/day]	10 Chl a/cell [pg/#]	11 Carotenoids/cell [pg/#]	12 PN/cell [pg/#/h]	13 Calc rate C/cell [pg/#/h]	14 NC/NP	15 NPQ	16 Duration [h]	17 Fv/Fm	18 Y	19 P ratio (PAR 800/PAR 150)	20 Calc rate ratio (PAR 800/PAR 150)	21 Calc rate/P (PAR 800/PAR 150)	22 sigma PSII [A²/quanta]	23 delta F/Fm [arbitrary units] (the apparent photochemical ef...)	24 rETR max [µmol e/m²/s]	25 Ik	26 Temp [°C]	27 Sal	28 pH (NBS, initial)	29 pH std dev [±] (NBS, initial)	30 AT [µmol/kg] (initial, Potentiometric titra...)	31 AT std dev [±] (initial, Potentiometric titra...)	32 DIC [µmol/kg] (initial, Calculated using CO2SYS)	33 DIC std dev [±] (initial, Calculated using CO2SYS)	34 CO2 [µmol/kg] (initial, Calculated using CO2SYS)	35 CO2 std dev [±] (initial, Calculated using CO2SYS)	36 [HCO3]- [µmol/kg] (initial, Calculated using CO2SYS)	37 [HCO3]- std dev [±] (initial, Calculated using CO2SYS)	38 [CO3]2- [µmol/kg] (initial, Calculated using CO2SYS)	39 [CO3]2- std dev [±] (initial, Calculated using CO2SYS)	40 CSC flag (initial, Calculated using sea...)	41 pH (total scale, initial, Calcula...)	42 CO2 [µmol/kg] (initial, Calculated using sea...)	43 pCO2water_SST_wet [µatm] (initial, Calculated using sea...)	44 fCO2water_SST_wet [µatm] (initial, Calculated using sea...)	45 [HCO3]- [µmol/kg] (initial, Calculated using sea...)	46 [CO3]2- [µmol/kg] (initial, Calculated using sea...)	47 DIC [µmol/kg] (initial, Calculated using sea...)	48 Omega Arg (initial, Calculated using sea...)	49 Omega Cal (initial, Calculated using sea...)	50 pH (NBS, after transfer)	51 pH std dev [±] (NBS, after transfer)	52 AT [µmol/kg] (after transfer, Potentiometri...)	53 AT std dev [±] (after transfer, Potentiometri...)	54 DIC [µmol/kg] (after transfer, Calculated us...)	55 DIC std dev [±] (after transfer, Calculated us...)	56 CO2 [µmol/kg] (after transfer, Calculated us...)	57 CO2 std dev [±] (after transfer, Calculated us...)	58 [HCO3]- [µmol/kg] (after transfer, Calculated us...)	59 [HCO3]- std dev [±] (after transfer, Calculated us...)	60 [CO3]2- [µmol/kg] (after transfer, Calculated us...)	61 [CO3]2- std dev [±] (after transfer, Calculated us...)	62 CSC flag (after transfer, Calculated us...)	63 pH (total scale, after transfer, ...)	64 CO2 [µmol/kg] (after transfer, Calculated us...)	65 pCO2water_SST_wet [µatm] (after transfer, Calculated us...)	66 fCO2water_SST_wet [µatm] (after transfer, Calculated us...)	67 [HCO3]- [µmol/kg] (after transfer, Calculated us...)	68 [CO3]2- [µmol/kg] (after transfer, Calculated us...)	69 DIC [µmol/kg] (after transfer, Calculated us...)	70 Omega Arg (after transfer, Calculated us...)	71 Omega Cal (after transfer, Calculated us...)
Emiliania huxleyi (phytoplankton)	High Ca	5		0	10.0	10.0	1							0.276											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	5		0	10.0	10.0	2							0.395											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	5		0	10.0	10.0	3							0.494											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	5		0	10.0	0.1	1							0.570											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	5		0	10.0	0.1	2							0.288											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	5		0	10.0	0.1	3							0.387											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca	5		1	10.0	10.0	1							0.427											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	5		1	10.0	10.0	2							0.425											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	5		1	10.0	10.0	3							0.381											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	5		1	10.0	0.1	1							0.366											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	5		1	10.0	0.1	2							0.351											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	5		1	10.0	0.1	3							0.263											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca	1, 3, 5		3	10.0	10.0	1	1.010	0.142	0.121	0.623	0.254	0.706	0.292		0.583					697				20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	1, 3, 5		3	10.0	10.0	2	1.046	0.145	0.126	0.500	0.440	0.802	0.240		0.584					859				20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	1, 3, 5		3	10.0	10.0	3	1.035	0.162	0.134	0.396	0.401	0.642	0.316		0.580					670				20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	1, 3, 5		3	10.0	0.1	1	0.901	0.086	0.141	0.198	0.016	0.080	0.153		0.587					746				20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	1, 3, 5		3	10.0	0.1	2	0.901	0.093	0.155	0.203	0.045	0.222	0.180		0.576					767				20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	1, 3, 5		3	10.0	0.1	3	0.896	0.088	0.149	0.220	0.022	0.101	0.156		0.578					657				20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca	1, 3, 5		6	10.0	10.0	1	1.112	0.127	0.151	0.454	0.201	0.443	0.353		0.576					759				20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	1, 3, 5		6	10.0	10.0	2	1.111	0.105	0.126	0.507	0.449	0.554	0.334		0.593					722				20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	1, 3, 5		6	10.0	10.0	3	1.059	0.108	0.129	0.535	0.281	0.840	0.350		0.584					677				20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	1, 3, 5		6	10.0	0.1	1	1.017	0.089	0.114	0.199	0.033	0.164	0.169		0.584					773				20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	1, 3, 5		6	10.0	0.1	2	1.000	0.086	0.114	0.195	0.053	0.272	0.186		0.581					746				20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	1, 3, 5		6	10.0	0.1	3	0.994	0.086	0.112	0.234	0.039	0.166	0.187		0.574					674				20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca	1, 3, 5		9	10.0	10.0	1	1.072	0.105	0.173	0.491	0.293	0.597	0.351		0.586					784				20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	1, 3, 5		9	10.0	10.0	2	1.106	0.110	0.171	0.545	0.314	0.577	0.371		0.596					673				20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	1, 3, 5		9	10.0	10.0	3	1.062	0.113	0.194	0.503	0.256	0.509	0.390		0.583					697				20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	1, 3, 5		9	10.0	0.1	1	0.913	0.107	0.122	0.260	0.033	0.128	0.298		0.591					747				20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	1, 3, 5		9	10.0	0.1	2	0.944	0.118	0.110	0.234	0.035	0.149	0.273		0.591					719				20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	1, 3, 5		9	10.0	0.1	3	0.905	0.101	0.114	0.309	0.055	0.177	0.293		0.585					657				20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	Low Ca	5		0	0.1	0.1	1							0.135											20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	5		0	0.1	0.1	2							0.133											20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	5		0	0.1	0.1	3							0.117											20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca-High Ca	5		0	0.1	10.0	1							0.034											20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca-High Ca	5		0	0.1	10.0	2							0.064											20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca-High Ca	5		0	0.1	10.0	3							0.068											20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	5		1	0.1	0.1	1							0.267											20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	5		1	0.1	0.1	2							0.205											20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	5		1	0.1	0.1	3							0.271											20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca-High Ca	5		1	0.1	10.0	1							0.343											20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca-High Ca	5		1	0.1	10.0	2							0.407											20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca-High Ca	5		1	0.1	10.0	3							0.347											20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	1, 3, 5		3	0.1	0.1	1	0.996	0.086	0.116	0.215	0.041	0.190			0.576					615				20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	1, 3, 5		3	0.1	0.1	2	0.992	0.078	0.109	0.210	0.052	0.246			0.598					752				20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	1, 3, 5		3	0.1	0.1	3	0.981	0.110	0.120	0.228	0.054	0.237			0.577					688				20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca-High Ca	1, 3, 5		3	0.1	10.0	1	0.966	0.087	0.112	0.323	0.337	1.044			0.591					507				20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca-High Ca	1, 3, 5		3	0.1	10.0	2	0.990	0.101	0.127	0.302	0.349	1.155			0.594					753				20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca-High Ca	1, 3, 5		3	0.1	10.0	3	0.952	0.120	0.131	0.342	0.382	1.116			0.584					673				20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	1, 3, 5		6	0.1	0.1	1	1.047	0.098	0.133	0.186	0.083	0.336	0.113		0.585					604				20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	1, 3, 5		6	0.1	0.1	2	1.072	0.082	0.132	0.246	0.070	0.299	0.131		0.594					765				20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	1, 3, 5		6	0.1	0.1	3	1.047	0.093	0.130	0.233	0.035	0.187	0.095		0.575					694				20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca-High Ca	1, 3, 5		6	0.1	10.0	1	0.981	0.119	0.157	0.419	0.400	0.953	0.211		0.592					571				20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca-High Ca	1, 3, 5		6	0.1	10.0	2	1.005	0.112	0.147	0.383	0.367	0.958	0.194		0.601					788				20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca-High Ca	1, 3, 5		6	0.1	10.0	3	1.002	0.119	0.153	0.413	0.369	0.894	0.224		0.593					695				20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	1, 3, 5		9	0.1	0.1	1	1.085	0.106	0.112	0.264	0.038	0.145	0.117		0.573					550				20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	1, 3, 5		9	0.1	0.1	2	1.076	0.099	0.114	0.263	0.048	0.183	0.134		0.598					760				20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	1, 3, 5		9	0.1	0.1	3	1.089	0.098	0.136	0.266	0.048	0.179	0.123		0.579					657				20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca-High Ca	1, 3, 5		9	0.1	10.0	1	1.054	0.127	0.172	0.333	0.408	1.226	0.198		0.587					677				20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca-High Ca	1, 3, 5		9	0.1	10.0	2	1.053	0.119	0.166	0.392	0.361	0.921	0.176		0.604					772				20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca-High Ca	1, 3, 5		9	0.1	10.0	3	1.017	0.138	0.162	0.344	0.411	1.195	0.196		0.587					687				20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	2, 4	2090	6	10.0	10.0	1	0.563			0.119	0.012		0.116											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	2, 4	2090	6	10.0	10.0	2	0.594			0.124	0.006		0.153											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	2, 4	2090	6	10.0	10.0	3	0.616			0.109	0.016		0.178											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	2, 4	2090	6	10.0	10.0	4	0.536						0.160											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	2, 4	2090	6	10.0	10.0	5	0.544						0.110											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	2, 4	2090	6	10.0	10.0	6	0.494						0.145											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	2, 4	2090	6	10.0	0.1	1	0.543			0.113	-0.009		0.141											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	2, 4	2090	6	10.0	0.1	2	0.583			0.108	0.003		0.113											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	2, 4	2090	6	10.0	0.1	3	0.577			0.103	-0.001		0.081											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	2, 4	2090	6	10.0	0.1	4	0.555						0.115											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	2, 4	2090	6	10.0	0.1	5	0.569						0.107											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	2, 4	2090	6	10.0	0.1	6	0.565						0.217											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca	2, 4	1516	6	10.0	10.0	1	0.821			0.190	0.012		0.136											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	2, 4	1516	6	10.0	10.0	2	0.747			0.193	0.029		0.189											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	2, 4	1516	6	10.0	10.0	3	0.813			0.223	-0.007		0.201											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	2, 4	1516	6	10.0	10.0	4	0.831						0.179											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	2, 4	1516	6	10.0	10.0	5	0.814						0.133											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	2, 4	1516	6	10.0	10.0	6	0.828						0.109											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	2, 4	1516	6	10.0	0.1	1	0.760			0.237	-0.003		0.126											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	2, 4	1516	6	10.0	0.1	2	0.807			0.293	-0.022		0.148											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	2, 4	1516	6	10.0	0.1	3	0.738			0.168	0.040		0.172											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	2, 4	1516	6	10.0	0.1	4	0.786						0.155											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	2, 4	1516	6	10.0	0.1	5	0.794						0.127											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca	2, 4	1516	6	10.0	0.1	6	0.788						0.140											20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca	7			10.0	10.0	1								0.0	0.552	0.513								20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	7			10.0	10.0	2								0.0	0.564	0.514								20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	7			10.0	10.0	3								0.0	0.559	0.526								20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	7			10.0	10.0	1								0.5	0.433	0.267								20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	7			10.0	10.0	2								0.5	0.439	0.285								20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	7			10.0	10.0	3								0.5	0.455	0.297								20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	7			10.0	10.0	1								1.0	0.412	0.271								20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	7			10.0	10.0	2								1.0	0.411	0.269								20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	7			10.0	10.0	3								1.0	0.430	0.272								20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	7			10.0	10.0	1								1.5	0.400	0.273								20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	7			10.0	10.0	2								1.5	0.395	0.275								20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	7			10.0	10.0	3								1.5	0.403	0.294								20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	7			10.0	10.0	1								2.0	0.406	0.232								20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	7			10.0	10.0	2								2.0	0.409	0.244								20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	7			10.0	10.0	3								2.0	0.358	0.265								20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	7			0.1	0.1	1								0.0	0.553	0.509								20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	7			0.1	0.1	2								0.0	0.562	0.539								20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	7			0.1	0.1	3								0.0	0.559	0.544								20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	7			0.1	0.1	1								0.5	0.416	0.202								20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	7			0.1	0.1	2								0.5	0.412	0.221								20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	7			0.1	0.1	3								0.5	0.421	0.197								20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	7			0.1	0.1	1								1.0	0.251	0.213								20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	7			0.1	0.1	2								1.0	0.297	0.224								20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	7			0.1	0.1	3								1.0	0.295	0.210								20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	7			0.1	0.1	1								1.5	0.319	0.169								20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	7			0.1	0.1	2								1.5	0.294	0.161								20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	7			0.1	0.1	3								1.5	0.278	0.179								20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	7			0.1	0.1	1								2.0	0.313	0.137								20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	7			0.1	0.1	2								2.0	0.273	0.139								20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	7			0.1	0.1	3								2.0	0.241	0.148								20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	7			10.0	10.0	1											2.377	4.867	2.062					20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	7			10.0	10.0	2											2.496	4.276	1.725					20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca	7			10.0	10.0	3											2.429	5.421	2.247					20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	7			0.1	0.1	1											1.435	2.203	1.524					20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	7			0.1	0.1	2											1.415	2.515	1.763					20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca	7			0.1	0.1	3											1.355	3.434	2.514					20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca				10.0	10.0	1															0.198	119.877	606.817	20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca				10.0	10.0	2															0.194	129.622	667.551	20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca				10.0	10.0	3															0.193	125.502	648.595	20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca				10.0	10.0	4															0.186	118.393	636.953	20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca				10.0	10.0	5															0.188	119.139	634.770	20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca				10.0	10.0	6															0.189	123.930	654.349	20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca				10.0	10.0	7															0.191	123.522	646.635	20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca				10.0	10.0	8															0.196	124.479	633.849	20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca				10.0	10.0	9															0.204	136.902	672.052	20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca				10.0	0.1	1															0.237	93.049	392.482	20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca				10.0	0.1	2															0.246	102.023	414.113	20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca				10.0	0.1	3															0.227	107.236	472.268	20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca				10.0	0.1	4															0.248	92.713	373.540	20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca				10.0	0.1	5															0.280	104.958	375.014	20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca				10.0	0.1	6															0.233	104.502	448.316	20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca				10.0	0.1	7															0.210	104.910	499.581	20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca				10.0	0.1	8															0.237	97.603	412.665	20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	High Ca-Low Ca				10.0	0.1	9															0.239	103.516	433.319	20	35	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.07	13	413	412	1993	208	2215	3.24	4.98
Emiliania huxleyi (phytoplankton)	Low Ca				0.1	0.1	1															0.196	106.876	545.600	20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca				0.1	0.1	2															0.213	105.995	498.496	20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca				0.1	0.1	3															0.199	112.711	565.921	20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca				0.1	0.1	4															0.192	110.988	577.139	20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca				0.1	0.1	5															0.197	115.255	586.535	20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca				0.1	0.1	6															0.182	112.167	617.814	20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca				0.1	0.1	7															0.215	97.875	455.023	20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca				0.1	0.1	8															0.183	109.210	595.960	20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca				0.1	0.1	9															0.184	106.643	580.776	20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca-High Ca				0.1	10.0	1															0.189	116.886	618.445	20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca-High Ca				0.1	10.0	2															0.192	123.595	643.064	20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca-High Ca				0.1	10.0	3															0.190	124.027	653.497	20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca-High Ca				0.1	10.0	4															0.186	119.802	644.054	20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca-High Ca				0.1	10.0	5															0.190	122.159	643.653	20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca-High Ca				0.1	10.0	6															0.188	122.047	648.435	20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca-High Ca				0.1	10.0	7															0.215	130.071	604.569	20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca-High Ca				0.1	10.0	8															0.191	118.887	621.425	20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15
Emiliania huxleyi (phytoplankton)	Low Ca-High Ca				0.1	10.0	9															0.193	117.198	606.383	20	35	8.19	0.01	2499	23	2209	24	13	0.5	1989	24	207	4	26	8.08	13	408	407	2015	215	2244	3.35	5.15	8.20	0.01	2537	13	2237	13	13	0.4	2008	14	216	14	26	8.08	13	408	407	2015	215	2244	3.35	5.15